SE2B Workshop on “Visualising the dynamic landscape of thylakoid membranes”
QMUL, 10-14 July 2017
Talks will take place in the Graduate Centre, QMUL Mile End Campus
(Room GC205 Monday - Thursday and GC203 on Friday).
Note the Graduate Centre is building no. 18 on the map below, and the first talk (welcome to the course) is at 9.30 am on Monday. There is a nice coffee bar in the ground floor of the Graduate Centre!
In case of problems please contact Prof Conrad Mullineaux: e-mail , mobile +44 7903187604 or come to reception in the first floor of the GE Fogg building (no. 13 on the map) and ask for him there.
Schedule:
SE2B Workshop on “Visualising the dynamic landscape of thylakoid membranes”
QMUL, 10-14 July 2017
Contributors:
Conrad Mullineaux (QMUL) - Lectures and fluorescence microscopypracticals
Giulia Mastroianni(QMUL) - electron microscopy practicals
Helder Carmen (QMUL) – fluorescence microscopy practical
Roman Kouril(Olomouc) - lectures
Egbert Boekema(Groningen) - lectures
Matt Johnson (Sheffield) – lectures
Participants:
Alba Blazquez (Frankfurt)
Claudio Calvaruso(Frankfurt)
Steffen Grebe (Turku)
MoontahaMahbub(QMUL)
Vincenzo Mascoli (Amsterdam)
Fernando Muzzopappa(Saclay)
Alexandros Polyzois(Paris)
BabooNarottamsingRamdour(Amsterdam)
Francesco Saccon(QMUL)
Christo Schiphorst(Verona)
Simona Streckaite(Saclay)
Overall structure: We will have 2 lectures and 1 practical session per day, Monday- Friday. Lectures will be 9:30 – 10:30 and 11-12. Practicals will be either 1-3 pm (Group A) or 3-5 pm (Group B).
Monday 10 July
Lecture 1(GC205) – Welcome, Introduction to the course and reminder about the MOOC! – Conrad Mullineaux
Lecture 2(GC205) – Overview of microscopic techniques – Conrad Mullineaux PLUS Preparation of samples for thin-section electron microscopy – Giulia Mastroianni
Practical– Sample preparation for thin-section electron microscopy (Giulia Mastroianni)
Group A – 1-3 pm
Group B - 3-5 pm
Tuesday 11 July
Lecture 3(GC205) – Thin-section and immunogold EM (Egbert Boekema)
Lecture 4(GC205) – Negative staining and single particle EM (Roman Kouril/Egbert Boekema)
Practical– freeze-fracture electron microscopy on chloroplasts (Giulia Mastroianni)
Group A – 1-3 pm
Group B – 3 -5 pm
Wednesday 12 July
Lecture 5 (GC205) - Cryo-electron tomography (RomanKouril/EgbertBoekema)
Lecture 6 (GC205) – Freeze-fracture electron microscopy and data analysis (Matt Johnson)
Practical- Confocal and 2-photon microscopy (Conrad Mullineaux/Helder Carmen)
Group A 1-3 pm
Group B 3-5 pm
Thursday 13 July
Lecture 7(GC205) – Fluorescence microscopy – confocal, TIRF and super-resolution (ConradMullineaux)
Lecture 8(GC205) – Atomic force microscopy (Matt Johnson)
Practical- Confocal microscopy and FRAP (Conrad Mullineaux)
Group A 1-3 pm
Group B 3-5 pm
Friday 14 July
Lecture 9(GC203) Dynamic fluorescence microscopy – single-particle tracking and FRAP(ConradMullineaux)
Lecture 10(GC203) FLIM (Conrad Mullineaux)
Practical– visualising samples under the EM (Giulia Mastroianni)
Group A 1-3 pm
Group B 3-5 pm
From 5 pm: Drinks and snacks in the Hub, GE Fogg Building