SCREENING THE FRUIT JUICE OF Morinda citrifolia FOR

ANTIDIABETICACTIVITY

SYNOPSIS FOR

M.PHARM DISSERTATION

SUBMITTED TO

RAJIVGANDHIUNIVERSITY OF HEALTH SCIENCES,

BANGALORE, KARNATAKA.

BY

MOHAMMED FARAZ,

DEPARTMENT OF PHARMACOLOGY,

NARGUNDCOLLEGE OF PHARMACY,

BANGALORE-560085.

(2008-2010)

RAJIVGANDHIUNIVERSITY OF HEALTH SCIENCES

KARNATAKA, BANGALORE.

ANNEXURE-II

PROFORMA FOR REGISTRATION OF SUBJECT FOR P.G DISSERTATION

1. / Name of the candidate and address(in block letters) /

MOHAMMED FARAZ

NARGUND COLLEGE OF PHARMACY

M.PHARM (2008-10)PHARMACOLOGY
DATTATREYA NAGAR,II MAIN,
100 FT RING ROAD,BSK III STAGE,
BANGLORE-85
2. /

Name of the institution

/

NARGUNDCOLLEGE OF PHARMACY

NARGUND COLLEGE OF PHARMACY

DATTATREYA NAGAR,II MAIN,
100 FT RING ROAD,BSK III STAGE,
BANGLORE-85
3. /

Course of study and subject

/

MASTER OF PHARMACY IN

PHARMACOLOGY

4. /

Date of the admission

/ 11 June 2008
5.
6. /

Title of the topic:

SCREENING THE FRUIT JUICE OF Morinda citrifolia FOR ANTIDIABETIC ACTIVITY
BRIEF RESUME OF THE INTENDED WORK
6.1 NEED FOR THE STUDY
Diabetes mellitus isa group of metabolic disorders characterized by hyperglycemia; is associated with abnormalities in carbohydrates, fat and protein metabolism that results in chronic complications including micro vascular, macro vascular, and neuropatic disorders.
Research and drug development efforts over past several decades have provided valuable information that applies directly to improving outcomes in patients with Diabetes mellitus and have expanded the therapeutic armamentarium.1
The currently available oral antihyperglycemic agents for clinical use have characteristic profile of side effects.2Management of diabetes with agents devoid of any side effects is still a challenge to the medical system. This has led to an increase in the demand for natural products with Antihyperglycemic activity having fewer side effects.In accordance with the recommendations of the WHO3 expert committee on diabetes mellitus, an investigation of Antihyperglycemic agents of plant origin used in traditional medicine seems important. Many herbs and plant products have been screened for Antihyperglycemic activity. 4,5,6,
Morinda citrifolia L. (Rubiaceae) known as “Noni”or ‘Indian Mulberry (in India)’, is a small tree that grows widely across Polynesia. Noni juice which is made from the fruits of Morinda citrifoliaplant is today widely used for its purported ability to prevent lifestyle related diseases such as Diabeties, Hypertension, cardiopathy and cerebral apoplexy caused by arteriosclerosis. 7.
Hawaiians commonly prepare tonics using Morinda citrifolia L. (Rubiaceae) designed to treat diabetes, stings, burns and fish poisoning.8In this research work an attempt is being made to discover newer drug of plant origin with antihyperglycemic which will benefit patients suffering from Diabetes mellitus. For this its nessesary to evaluate the hypoglycemic activity in naturally occurring substances. In this project AntiDiabetic activity of Morinda citrifolia fruits will be evaluated .
7.
8 / 6.2REVIEW OF LITERATURE:
The wound healing activity of Morinda citrifolia fruit juice on diabetes induced rats is studied and is demonstrated that the juice of Morinda citrifolia fruits significantly reduces blood sugar levels and hastens wound healing in diabetic rats.9
Morinda citrifolia may be used as Antiobesity agent in body weight management.10
Chemical Constituents of Morinda citrifolia Roots Exhibit Hypoglycemic
Effects in Streptozotocin-Induced Diabetic Mice11
Noni-ppt may suppress tumour growth through activation of the host immune system12
The lyophilized aqueous extract of roots of Morinda citrifolia was evaluated for analgesic and behavior effects in mice. 13
The crude ethanol extract and hexane fraction from Morinda citrifolia Linn. (Rubiaceae) show antitubercular activity.14
Use of Noni juice to combat fatigue, improve endurance and increase overall physical performace.15
Noni juice is effective in protecting the liver from extrinsic toxin exposure.16
6.3 OBJECTIVE OF THE STUDY
The main objective of the proposed work is to evaluate the beneficial effects of Morinda citrifolia for its Anti hyperglycemic effect
MATERIAL AND METHODS
7.1Source Of Data :
Whole work is planned to generate data from laboratory studies i.e. experiments are performed as described in references. Experimental studies in journals and in text books available with college and various institutions.
7.2METHOD OF COLLECTION OF DATA:
The data collected will be based on animal experimentation as per the parameters studied.
METHODOLOGY :
I. ALLOXAN-INDUCED diabetes in Rats.
Animal:Albino Wister Rats
( 6 groups of 10 animals each)
Group I: Normal control
Group II: Diabetes induced control
Group III: Diabetes induced + Standard drug treated.
Group IV: Diabetes induced +Test drug treated Dose I.
Group V: Diabetes induced +Test drug treated Dose II.
. Group VI: Test drug treated Normal group.
Parameters to be evaluated
1. Plasma glucose levels
2. HDL, LDL, Totalcholesterol.
II.Corticosteroids induced insulin resistance in rats.
Animal: Albino Wister Rats
( 6 groups of 10 animals each)
Group I: Normal control
Group II: Diabetes induced control
Group III: Diabetes induced + Standard drug treated.
Group IV: Diabetes induced +Test drug treated Dose I.
Group V: Diabetes induced + Test drug treated Dose II.
. Group VI: Test drug treated Normal group.
Parameters to be evaluated
1. Blood glucose level.
2. Plasma triglycerides.
3. Body weight.
III. EFFECT OF Morinda citrifolia ON α - GLUCOSIDASE ENZYME.
Animal: Albino Wister Rats
( 2 groups of 6 animals each)
Group I: Normal
Group II: Drug treated
Parameter to be evaluated
1.Enzyme activity in the presence of the drug will be evaluated
STATISTICAL ANALYSIS:
Data will be analyzed using one way analysis of variance (ANOVA)
7.3 Does the study require any investigation to be conducted on patients
Or other humans or animals? If so please describe briefly
Yes, The above study requires investigation on animals. The effect of drug will be studied on
Various parameters using rat as animal model.
7.4 Has ethical clearance been obtained from your institution in case of 7.3?
Application has been submitted to “Institutional animal ethics committee” of Nargund
College of Pharmacy
REFERENCES:
1. Triplitt L, Reasner A, Isley L. Diabetes mellitus. In:Dipiro T, Talbert L, Yee C, Matzke R, Wells G,Posey L,editors. Pharmacotherapy a pathophysiologic approach. 6thed.United states of America: Mc Graw Hill publications; 2005. p. 1334.
2.Holman RR, Turner RC. Oral agents and insulin in the treatment of NIDDM. In: Pickup J, Willians G, editors. Textbook of Diabetes.Oxford: Blackwell; 1991.
3. The WHO Expert Committee on Diabetes mellitus. Technical Reports series 646. Geneva: World Health Organization; 1980.
4. Bailey CJ, Day C. Traditional plants medicines as treatment for diabetes. Diabetes Care. 1989;12:553-64.
5. Mukherjee SK. Indigenous drugs in diabetes mellitus. J Diab Assoc India.1981;XXI:97-106.
6. Ajgaonkar SS. Herbal drugs in the treatment of diabetes a review. IDF Bulletin. 1979;24:10-7.
7 Kohei, Wakako, Sachiko, Rie I, Shogo, Toshiko. Chemical constituents of Morinda citrifolia Roots Exhibit hypoglycemic effects in Streptozotocin-induced diabetic mice. Biol Pharm Bull.2008 May;31(5):935-8.
8. Freepatentsonline. [homepage on the internet].USA: Freepatentsonline; [cited 2008 Nov 26].search Morida citrifolia;[about 2 screens]. Available from:
9. Nayak S,Evaluation of the wound-healing activity of the medicinal plants from Trinidad
on streptozotocin-induced diabetic rats
10.Pak-Dek MS, Abdul-Hamid A, Osman A, Soh CS.Inhibitory effect of Morinda citrifolia L. On lipoprotein lipase activity.J Food Sci. 2008 Oct;73(8):C595-8.
11. Kohei, Wakako, Sachiko, Rie I, Shogo, and Toshiko. Chemical constituents of Morinda citrifolia Roots Exhibit Hypoglycemic Effects in Streptozotocin-Induced DiabeticMice. Biol Pharm Bull.2008 May;31(5):935-8
12. Hirazumi A, Furusawa E. An immunomodulatory polysaccharide-rich substance from the fruit juice of Morinda citrifolia (noni) with antitumour activity.Phytother Res. 1999 Aug;13(5):380-7
13.Younos C, Rolland A, Fleurentin J, Lanhers MC, Misslin R, Mortier F. Analgesic and behavioural effects of Morinda citrifolia.Planta Med. 1990 Oct;56(5):430-4.
14.Saludes JP, Garson MJ, Franzblau SG, Aguinaldo AM. Antitubercular constituents from the hexane fraction of Morinda citrifolia Linn. (Rubiaceae).Phytother Res. 2002 Nov;16(7):683-5.
15.Ma DL, West BJ, Su CX, Gao JH, Liu TZ, Liu YW. Evaluation of the ergogenic potential of noni juice.Phytother Res. 2007 Nov;21(11):1100-1
16.Wang MY, Nowicki D, Anderson G, Jensen J, West B. Liver protective effects of Morinda citrifolia (Noni).Plant Foods Hum Nutr. 2008 Jun;63(2):59-63. Epub 2008 Mar 4.
17. Vogel H, editor. Drug Discovery and Evaluation Pharmacological Assays. 2nd edition.New yor: Springer-VerlagBerlinHeidelberg. [CD-ROM] pp, 950.
18.ShalamMd., HarishMS, FarhanaSA. Prevention of dexamethasone- and fructose-induced insulin resistance in rats by SH-01D, a herbal preparation. Indian Journal of pharmacology.2006; 36(6),419-422
09. / SIGNATURE OF THE CANDIDATE
10. / REMARKS OF THE GUIDE / RECOMMENDED
11. / NAME AND DESIGNATION
11.1 NAME OF THE GUIDE / PROF. D.S PURANIK
PROFESSOR
DEPARTMENT OF PHARMACOLOGY
NARGUNDCOLLEGE OF PHARMACY, BANGALORE-85.
11.2 SIGNATURE
11.3 CO-GUIDE (IF ANY)
11.4SIGNATURE / .
11.5HEAD OF THE
DEPARTMENT / Dr. HRISHI KESHAVAN
PROFESSOR
DEPARTMENT OF PHARMACOLOGY
NARGUNDCOLLEGE OF PHARMACY, BANGALORE-85.
11.6 SIGNATURE
12. / 12.1 REMARKS OF THE
CHAIRMAN & PRINCIPAL
12.2 SIGNATURE