Curriculum Vitae
Personal Information
First Name: Dzmitry Gender: male
Last Name: Mukha Nationality: Belarus
Date of Birth: 27/01/1986 Email:
Contact Number: +375 29 5629979 Contact Address: Russiyanov Street, 50 - 405, 220141, Minsk, Belarus
Professional Profile
Current Position: Research Fellow
Current Research: Current research is devoted to developing pipelines for in silico and in vitro screening of new potential drug compounds. The work comprises creation of genetic constructions, expression, and chromatographic purification of recombinant proteins from different sources including humans. Computational biology tasks include construction of precise spatial models of protein using sophisticated molecular dynamics technics and performing screening procedures based on both numerical descriptors and spatial properties of chemical compounds. Few original algorithms of protein properties modeling were developed. Results of computational procedures are validated by different spectroscopy experiments with recombinant proteins obtained. The main focus of current studies is investigation of human cytochromes performing xenobiotics conversion.
Affiliated Institution: Tauber Bioinformatics Research Center, Haifa University
Education
2012/09 Sofia School of Protein Science: Structure and dynamics of biological macromolecules, FEBS Lecture Course, Sofia, Bulgaria
2010/10 BioCrys2010, FEBS Practical and Lecture Course, Fundamentals of Modern Methods in BioCrystallography, Oeiras, Portugal
2008/11 – 2011/11 PhD student in Biochemistry, Institute of Bioorganic Chemistry, NAS of Belarus
2008/06 Graduated from Department of Genetics, Biological Faculty of Belarusian State University, Minsk, Republic of Belarus (‘Heterological expression of human cytochrome P450 3A4 and its physical-chemical characterization’)
Employment history
2012/06 – present Consultant, Tauber Bioinformatics Research Center, Haifa University
2011/11 – present Research Fellow, Laboratory of Protein Engineering, Institute of Bioorganic Chemistry, National Academy of Sciences, Minsk, Republic of Belarus.
2008/08 – 2011/11 Junior Research Fellow, Laboratory of Protein Engineering, Institute of Bioorganic Chemistry, National Academy of Sciences, Minsk, Republic of Belarus.
Academic Awards
2010/12 Gratitude from President of National Academy of Sciences
2011/01 ‘Best first publication’ prize of National Academy of Sciences
Professional Qualifications
Computer skills: Experienced in using/administration of rpm-based Linux (Fedora 12 -17, Red Hat v.5.x, v.6.x, CentOS 5.x, 6.x), programming on Java, bash scripting, advanced user of PBS/Torque cluster management middleware, GRID Unicore middleware, UCSF AMBER 10/11/12, GAMESS, UCSF Dock v.6.5, user of MOSFLM, CCP4.
Experimental methods: Electron spectroscopy, cloning, recombinant protein expression, protein chromatographic purification, biochemical assays, mass spectroscopy.
Languages: English, Russian, Belarusian.
Selected publications
Articles:
· Ershov P, Mezentsev Y, Gnedenko O, Mukha D, Yantsevich A, Britikov V, Kaluzhskiy L, Yablokov E, Molnar A, Ivanov A, Lisitsa A, Gilep A, Usanov S, Archakov A. Protein interactomics based on direct molecular fishing on paramagnetic particles: Experimental simulation and SPR validation. Proteomics. 2012 Sep 24. doi: 10.1002/pmic.201200135. [Epub ahead of print]
· Mukha D. V., Feranchuk S. I., Gilep A. A., Usanov S. A. Molecular Modeling of Human Lanosterol 14α-Demethylase Complexes with Substrates and Their Derivatives. Biochemistry (Moscow), 2011, V. 76, I. 2, pp. 175-85.
Conference abstracts:
· Mukha, D. V., S. A. Usanov. A novel method of protein-solvent binding energy evaluation via analysis of molecular dynamics trajectories. The 4th International IMBG Conference for Young Scientists «Molecular biology: advances and perspectives», Kyiv, Ukraine, September 14-17, 2011. — P. 199.
· Mukha, D. V., Usanov, S. A. Elucidation of the functional role of amino acids forming the substrate-binding site of human CYP51A1 by molecular modeling and sequence analysis. 17th International Conference on Cytochrome P450, Manchester, UK, 26th-30th of June 2011. – Manchester, UK, 2011. – P. 23.
· Gilep A. A., Shpak S. V., Dvornikov A. C., Mukha D. V., Usanov S. A. Insights into the specificity of vitamin D binding proteins. 17th International Conference on Cytochrome P450, Manchester, UK, 26th-30th of June 2011. – Manchester, UK, 2011. – P. 74.
· Strushkevich N. V., Mukha D. V., Gilep A. A., Park H.-W., Usanov S. A. Structural basis of human CYP51 inhibition by antifungal azoles. 45th Meeting of the Polish Biochemical Society, Wisla, Poland, September 20 – 23, 2010, Slaski Medical University in Katowichi, p. 55.