Saladin's 02/14/02Ch. 4 TermsPost-copyedit

1)mendelian genetics

2)cytogenetics

3)molecular genetics

4)genomic medicine

5)deoxyribonucleic acid (DNA)

Insight 4.1 Medical History

Miescher and the Discovery of DNA

Swiss biochemist Johann Friedrich Miescher (1844–95) was one of the first scientists intent on identifying the hereditary material in nuclei. In order to isolate nuclei with minimal contamination, Miescher chose to work with cells that have large nuclei and very little cytoplasm. At first he chose white blood cells extracted from the pus in used bandages from a hospital; later, he used the sperm of salmon—probably more agreeable to work with than used bandages! Miescher isolated an acidic substance rich in phosphorus, which he named nuclein. His student, Richard Altmann, later called it nucleic acid—a term we now use for both DNA and RNA. Miescher correctly guessed that “nuclein” (DNA) was the hereditary matter of the cell, but he was unable to provide strong evidence for this conjecture, and his work was harshly criticized. He died of tuberculosis at the age of 51.

6)nucleoli

7)chromatin

8)chromosomes

9)nucleosomes

10)histones

11)nucleotides

12)nitrogenous base

13)cytosine (C)

14)thymine (T)

15)uracil (U)

16)adenine (A)

17)guanine (G)

18)base pairs

19)law of complementary base pairing

20)gene

The essential function of DNA is to serve as a code for the structure of polypeptides synthesized by a cell. A gene is a DNA nucleotide sequence that codes for one polypeptide. The next section of this chapter explains in detail how the genes direct polypeptide synthesis. All the genes of one person are called the genome (JEE-nome); geneticists estimate that a human has about 35,000 genes. These account for only 3% of our DNA; the other 97% does not code for anything. Some of the noncoding DNA serves important organizing roles in the chromatin, and some of it is useless “junk DNA” that has accumulated over the course of human evolution. The latest triumph of molecular genetics is the human genome project, an enormous multinational effort that led to the mapping of the base sequence of the entire human genome. Its completion (in all but some fine details) in June 2000 was hailed as a scientific achievement comparable to putting the first man on the moon.

21)messenger RNA (mRNA)

22)ribosomal RNA (rRNA)

23)transfer RNA (tRNA)

24)genetic code

25)base triplet

26)codon

27)stop codons

28)start codon

29)transcription

30)RNA polymerase

31)translation

32)anticodon

33)polyribosome

34)signal peptide

35)chaperones

36)posttranslational modification

37)transport vesicles

38)Golgi vesicles

39)secretory vesicles

40)DNA helicase

41)DNA polymerase

42)semiconservative replication

43)mutations

44)cell cycle

45)G1 first gap phase

46)S synthesis phase

47)G2 second gap phase

48)M mitotic phase

49)interphase

50)G0 (G-zero) phase

51)mitosis

52)prophase

53)chromatids

54)centromere

55)spindle fibers

56)kinetochore

57)metaphase

58)mitotic spindlewith

59)anaphase

60)telophase

61)cytokinesis

62)growth factors

63)contact inhibition

64)heredity

65)karyotype

66)homologouschromosomes

67)sex chromosomes

68)autosomes

69)germ cells

70)somatic cells

71)diploid

72)haploid

73)genome

74)Human Genome Project (HGP)

75)genomics

76)single-nucleotide polymorphisms

77)genomic medicine

78)locus

79)alleles

80)dominant

81)recessive

82)homozygous

83)heterozygous

84)genotype

85)phenotype

86)carriers

87)multiple alleles

88)gene pool

89)codominant

90)incomplete dominance

91)polygenic (multiple-gene) inheritance

92)pleiotropy

93)sex-linked traits

94)penetrance