Roman Fischercurriculum Vitae

Roman FischerCurriculum Vitae

Roman Fischer

TDI Mass Spectrometry Laboratory
Target Discovery Institute
Nuffield Department of Clinical Medicine
Oxford University
Roosevelt Drive
Oxford OX3 7FZ, UK

CURRENT POSITION

Oct.-2013 – nowHead of Proteomics Research Facility

(Target Discovery Institute, NDM, Oxford)

PREVIOUS POSITIONS

April 2009 – Sept 2013Postdoctoral Researcher

(Benedikt Kessler, TDI, University of Oxford)

Jan. 2008 – March 2009MRC Career Development Fellow

MRC Phosphorylation Unit, Dundee

Laboratory of Professor Sir Philip Cohen

EDUCATION

Nov. 2004 – Dec. 2007Dr. rer. nat.: “Characterisation of the Internalin A mediated signal transduction in epithelial cells” (Thesis in German), supervised by Prof. Lothar Jänsch

Helmholtz Centre for Infection Research

Division of Cell Biology

Cellular Proteomics Group

Oct. 2004Diploma in Biotechnology

Technische Universität Braunschweig, Germany

March 2004 – Sept. 2004Diploma thesis at the GBF Braunschweig, Proteomics Group: “Enrichment and Identification of Phosphopeptides after Induction of eukaryotic host cells by putative and known virulence factors of Listeria monocytogenes”

Oct. 2000 Bachelor (Vordiplom) in Biotechnology

Technische Universität Braunschweig, Germany

Bachelor thesis: “Gel-less quantification of Proteins using MCAT and ICAT™“

PUBLICATIONS (*(shared) first author publications), 215.14 impact points

see also

Welker F, Fischer R, et al., “Ancient Proteins Demonstrate South American Ungulates are Laurasiatheres, not Afrotheres.“ Nature, 2015 Mar 18. doi: 10.1038/nature14249.

Markkanen E, Fischer R, Ledentcova M, Kessler BM, Dianov GL, “Cells deficient in base-excision repair reveal cancer hallmarks originating from adjustments to genetic instability.“ Nucleic Acids Research 03/2015; DOI: 10.1093/nar/gkv222

Lugli EB, Correia RE, Fischer R, Lundberg K, Bracke KR, Montgomery AB, Kessle BMr, Brusselle GG, Venables PJ “Expression of citrulline and homocitrulline residues in the lungs of non-smokers and smokers: implications for autoimmunity in rheumatoid arthritis“ Arthritis Research & Therapy. 01/2015; 17(1):9. DOI: 10.1186/s13075-015-0520-x

Valli A, Fischer R, et al., “Hypoxia Induces a Lipogenic Cancer Cell Phenotype via HIF1α-Dependent and -Independent Pathways.“ Oncotarget, 2015 Feb 10;6(4):1920-41.

*Fischer R, *Tomlinson P, *Zheng Y, Heidasch R, Gardiner C, Evetts S, Hu M; Wade-Martins R, Turner M, Morris J, Talbot K, Kessler B, Tofaris G (2015). "Identification of distinct circulating microvesicles/exosomes in Parkinson’s disease." Annals of Clinical and Translational Neurology. 2015, 02/2015; 2(a):353. DOI: 10.1002/acn3.175

*Fischer R, Kessler BM (2014). “Gel-aided sample preparation (GASP) - a simplified method for gel-assisted proteomic sample generation from protein extracts and intact cells.“ Proteomics. 2014 Dec 17. doi: 10.1002/pmic.201400436. [Epub ahead of print]

Warinner C, Hendy J, Speller C, Cappellini E, Fischer R, Trachsel C, Arneborg J, Lynnerup N, Craig OE, Swallow DM, Fotakis A, Christensen RJ, Olsen JV, Liebert A, Montalva N, Fiddyment S, Charlton S, Mackie M, Canci A, Bouwman A, Rühli F, Gilbert MT, Collins MJ (2014). “Direct evidence of milk consumption from ancient human dental calculus.“ Sci Rep. 2014 Nov 27;4:7104

Colak E, Leslie A, Zausmer K, Khatamzas E, Kubarenko AV, Pichulik T, Klimosch SN, Mayer A, Siggs O, Hector A, Fischer R, Klesser B, Rautanen A, Frank M, Hill AV, Manoury B, Beutler B, Hartl D, Simmons A, Weber AN (2014). “RNA and imidazoquinolines are sensed by distinct TLR7/8 ectodomain sites resulting in functionally disparate signaling events.“ J Immunol. 2014 Jun 15;192(12):5963-73.

*Fischer R, *Trudgian DC, Guo X, Kessler BM, Mirzaei H. (2014). “GOAT - A simple LC-MS/MS gradient optimization tool.“ Proteomics. 2014 Jun;14(12):1467-71.

Singleton RS, Liu-Yi P, Formenti F, Ge W, Sekirnik R, Fischer R, Adam J, Pollard PJ, Wolf A, Thalhammer A, Loenarz C, Flashman E, Yamamoto A, Coleman ML, Kessler BM, Wappner P, Schofield CJ, Ratcliffe PJ, Cockman ME. (2014). “OGFOD1 catalyzes prolyl hydroxylation of RPS23 and is involved in translation control and stress granule formation.” Proc Natl Acad Sci U S A. 2014 Feb 18.

Feng T, Yamamoto A, Wilkins SE, Sokolova E, Yates LA, Münzel M, Singh P, Hopkinson RJ, Fischer R, Cockman ME, Shelley J, Trudgian DC, Schödel J, McCullagh JS, Ge W, Kessler BM, Gilbert RJ, Frolova LY, Alkalaeva E, Ratcliffe PJ, Schofield CJ, Coleman ML (2014). “Optimal Translational Termination Requires C4 Lysyl Hydroxylation of eRF1.” Mol Cell. 2014 Feb 20;53(4):645-54.

Chen L, Fischer R, Peng Y, Reeves E, McHugh K, Ternette N, Hanke T, Dong T, Elliott T, Shastri N, Kollnberger S, James E, Kessler B, Bowness P (2014). “Critical Role of Endoplasmic Reticulum Aminopeptidase 1 in Determining the Length and Sequence of Peptides Bound and Presented by HLA-B27.” Arthritis Rheumatol. 2014 Feb;66(2):284-94.

*Fischer, R., P. Bowness and B. M. Kessler (2013). "Two birds with one stone: Doing metabolomics with your proteomics kit." Proteomics 13(23-24): 3371-3386.

Ternette, N., M. Yang, M. Laroyia, M. Kitagawa, L. O'Flaherty, K. Wolhulter, K. Igarashi, K. Saito, K. Kato, R. Fischer, A. Berquand, B. M. Kessler, T. Lappin, N. Frizzell, T. Soga, J. Adam and P. J. Pollard (2013). "Inhibition of mitochondrial aconitase by succination in fumarate hydratase deficiency." Cell Rep 3(3): 689-700.

Thezenas, M. L., H. Huang, M. Njie, A. Ramaprasad, D. C. Nwakanma, R. Fischer, K. Digleria, M. Walther, D. J. Conway, B. M. Kessler and C. Casals-Pascual (2013). "PfHPRT: a new biomarker candidate of acute Plasmodium falciparum infection." J Proteome Res 12(3): 1211-1222.

*Fischer, R., D. C. Trudgian, C. Wright, G. Thomas, L. A. Bradbury, M. A. Brown, P. Bowness and B. M. Kessler (2012). "Discovery of candidate serum proteomic and metabolomic biomarkers in ankylosing spondylitis." Mol Cell Proteomics 11(2): M111 013904.

Wright, C., S. Sibani, D. Trudgian, R. Fischer, B. Kessler, J. LaBaer and P. Bowness (2012). "Detection of multiple autoantibodies in patients with ankylosing spondylitis using nucleic acid programmable protein arrays." Mol Cell Proteomics 11(2): M9 00384.

Konietzny, R., R. Fischer, N. Ternette, C. A. Wright, B. W. Turney, A. Chakera, D. Hughes, B. M. Kessler and C. W. Pugh (2012). "Detection of BK virus in urine from renal transplant subjects by mass spectrometry." Clin Proteomics 9(1): 4.

Rotili, D., M. Altun, A. Kawamura, A. Wolf, R. Fischer, I. K. Leung, M. M. Mackeen, Y. M. Tian, P. J. Ratcliffe, A. Mai, B. M. Kessler and C. J. Schofield (2011). "A photoreactive small-molecule probe for 2-oxoglutarate oxygenases." Chem Biol 18(5): 642-654.

Adam, J., E. Hatipoglu, L. O'Flaherty, N. Ternette, N. Sahgal, H. Lockstone, D. Baban, E. Nye, G. W. Stamp, K. Wolhuter, M. Stevens, R. Fischer, P. Carmeliet, P. H. Maxwell, C. W. Pugh, N. Frizzell, T. Soga, B. M. Kessler, M. El-Bahrawy, P. J. Ratcliffe and P. J. Pollard (2011). "Renal cyst formation in Fh1-deficient mice is independent of the Hif/Phd pathway: roles for fumarate in KEAP1 succination and Nrf2 signaling." Cancer Cell 20(4): 524-537.

Singleton, R. S., D. C. Trudgian, R. Fischer, B. M. Kessler, P. J. Ratcliffe and M. E. Cockman (2011). "Quantitative mass spectrometry reveals dynamics of factor-inhibiting hypoxia-inducible factor-catalyzed hydroxylation." J Biol Chem 286(39): 33784-33794.

Trudgian, D. C., G. Ridlova, R. Fischer, M. M. Mackeen, N. Ternette, O. Acuto, B. M. Kessler and B. Thomas (2011). "Comparative evaluation of label-free SINQ normalized spectral index quantitation in the central proteomics facilities pipeline." Proteomics 11(14): 2790-2797.

Kochan, G., T. Krojer, D. Harvey, R. Fischer, L. Chen, M. Vollmar, F. von Delft, K. L. Kavanagh, M. A. Brown, P. Bowness, P. Wordsworth, B. M. Kessler and U. Oppermann (2011). "Crystal structures of the endoplasmic reticulum aminopeptidase-1 (ERAP1) reveal the molecular basis for N-terminal peptide trimming." Proc Natl Acad Sci U S A 108(19): 7745-7750.

Altun, M., H. B. Kramer, L. I. Willems, J. L. McDermott, C. A. Leach, S. J. Goldenberg, K. G. Kumar, R. Konietzny, R. Fischer, E. Kogan, M. M. Mackeen, J. McGouran, S. V. Khoronenkova, J. L. Parsons, G. L. Dianov, B. Nicholson and B. M. Kessler (2011). "Activity-based chemical proteomics accelerates inhibitor development for deubiquitylating enzymes." Chem Biol 18(11): 1401-1412.

*R. Fischer, *Hundertmark, C., T. Reinl, S. May, F. Klawonn and L. Jansch (2009). "MS-specific noise model reveals the potential of iTRAQ in quantitative proteomics." Bioinformatics 25(8): 1004-1011.

Abraham, W. R., A. J. Macedo, H. Lunsdorf, R. Fischer, S. Pawelczyk, J. Smit and M. Vancanneyt (2008). "Phylogeny by a polyphasic approach of the order Caulobacterales, proposal of Caulobacter mirabilis sp. nov., Phenylobacterium haematophilum sp. nov. and Phenylobacterium conjunctum sp. nov., and emendation of the genus Phenylobacterium." Int J Syst Evol Microbiol 58(Pt 8): 1939-1949.