Rev. No: 0391Sworksheet Can Be Downloaded From

January 2015HLA-A-B-DR Combi TrayPage 1 of 1

Rev. No: 0391SWorksheet can be downloaded from

Worksheet

PHOTO DOCUMENT

Olerup SSP®

Olerup SSP®HLA-A-B-DR Combi Tray

Prod. No:101.701-24/24u

101.701-06/06u

Lot No: 91S

Expiry Date: 2016-May-01

Name:______Sample ID:______

DNA Extract Date:______Conc.(ng/ul):______

Test Date: ______Review Date:______

Tested By: ______Reviewed By:______

Interpretation:______

Specificities and sizes of the PCR products of the 24 primer mixes used for HLA-A low resolution SSP typing.

Primer Mix / Size of spec. PCR product1 / Size of control band2 / HLA-A
serology3 / Amplified HLA-Aalleles4,5
16,7 / 120 bp,
145 bp,
225 bp / 800 bp / A1, A36 / *01:01:01:01-01:04N, 01:06-01:33, 01:35-01:138, 03:18, 03:135, 11:94, 11:112, 36:01-36:05
27 / 175 bp,
215 bp,
255 bp,
365 bp,
415 bp / 800 bp / A2, A19,
A28, A203, A210 / *02:01:01:01-02:01:15, 02:01:17-02:01:19, 02:01:21-02:01:81, 02:01:83-02:22:02, 02:24:01-02:35:01, 02:35:03-02:47, 02:49-02:77, 02:78w, 02:79:01-02:97:02, 02:99, 02:101:01-02:128, 02:130-02:414, 02:416-02:436, 02:438
38 / 205 bp,
235 bp / 1070 bp / A1, A3, A11, A32, A34, A36 / *01:12, 01:19, 01:21, 01:126, 02:338, 03:01:01:01-03:17:02, 03:19-03:74, 03:76-03:94, 03:96-03:134, 03:136-03:174, 11:03, 11:20, 11:25, 11:60, 11:130, 24:92, 32:04, 34:02:01-34:04, 34:07-34:10N, 36:02
4 / 190 bp / 800 bp / A1, A3, A11, A30, A36, A68 / *01:01:01:01-01:01:22, 01:01:24-01:01:47, 01:01:49-01:04N, 01:06-01:07, 01:09-01:11N, 01:13, 01:16N-01:18N, 01:20-01:29, 01:31N-01:33, 01:35-01:78, 01:80-01:98, 01:100-01:138, 02:78, 02:169, 03:12, 03:18, 03:88, 03:135, 11:01:01-11:01:48, 11:01:50-11:27, 11:29-11:52Q, 11:54-11:150, 26:19, 26:72, 30:08, 36:04, 68:13:01, 68:66
5 / 160 bp,
335 bp,
505 bp / 1070 bp / A3, A9, A23, A24, A2403, A29, A31, A32 / *03:15, 03:19, 03:30, 03:152, 11:139, 23:01:01-23:60, 24:02:01:01-24:11N, 24:13:01-24:15, 24:17-24:64, 24:66-24:210, 24:212-24:240N, 24:242-24:246, 29:07, 29:49, 31:08, 31:29, 32:05, 33:19, 33:53, C*04:01:03
69,12,13 / 135 bp,
200 bp / 800 bp / A9, A23, A24, A29, A80 / *23:01:01-23:56, 23:58-23:60, 24:24, 24:71, 29:07, 29:49, 31:29, 80:01:01:01-80:03, B*18:27
7 / 175 bp,
205 bp / 1070 bp / A2, A3, A9, A23, A24, A2403, A26 / *02:17:01w-02:17:02w, 11:139, 23:14:01-23:14:02, 24:02:01:01-24:11N, 24:13:01-24:13:02, 24:17-24:50, 24:54-24:56, 24:58-24:63, 24:66-24:91, 24:93, 24:95-24:113, 24:115-24:137, 24:139-24:187, 24:189-24:210, 24:212-24:221, 24:223-24:227, 24:229-24:240N, 24:242-24:246, 26:16, 33:19, 68:45
8 / 165 bp,
200 bp / 800 bp / A2, A3, A10, A11, A25, A26, A28, A32, A34, A66, A68, A69 / *01:51, 02:55, 03:24, 03:50, 11:10, 25:01:01-25:16, 25:18-25:23, 26:01:01-26:06, 26:08-26:15, 26:17-26:18, 26:20-26:43:02, 26:45-26:63, 26:65-26:71N, 26:73-26:88, 26:90-26:91, 26:93-26:94, 29:28, 32:15, 33:51, 34:01:01-34:11, 66:01:01-66:19, 68:01:01:01-68:102, 69:01-69:02
96,10 / 75 bp / 800 bp / A3, A25, A32 / *25:01:01-25:23, 32:01:01-32:01:13, 32:01:15-32:02, 32:04, 32:06-32:37, 32:39-32:58, B*07:81, B*08:52, B*18:67, B*38:41, B*53:05, B*53:16
106,12 / 80 bp,
240 bp / 800 bp / A10, A26, A43 / *01:43, 01:51, 11:17, 11:40, 26:01:01-26:02:01, 26:04, 26:07:01-26:20, 26:22-26:29, 26:31-26:43:02, 26:45-26:77, 26:79-26:91, 26:93-26:94, 33:13, 33:48, 43:01, 68:84
116,7 / 80 bp,
175 bp,
500 bp / 1070 bp / A1, A9, A10, A11, A26, A31, A34, A66 / *01:13, 01:28, 01:136, 03:63, 03:88, 11:01:01-11:01:48, 11:01:50-11:27, 11:29-11:52Q, 11:54-11:150, 24:19, 24:44, 26:03:01-26:03:02, 26:06, 26:21, 26:78, 26:92, 34:01:01-34:08, 34:10N-34:11, 66:01:01, 66:04-66:11, 66:13-66:14, 66:17-66:19, 69:02, 80:02
126 / 125 bp,
190 bp / 800 bp / A3, A10, A25, A26, A31, A34, A43, A66 / *02:309, 03:01:19, 11:11, 25:05-25:06, 26:09, 26:54, 26:91, 31:03-31:04, 34:01:01-34:11, 43:01, 66:02-66:03, 66:16
13 / 175 bp,
225 bp / 1070 bp / A1, A2, A3, A10, A25, A26, A34, A43, A66 / *01:01:56w, 01:13, 02:34-02:35:03, 02:56:01-02:56:02, 02:62, 02:103, 02:135, 03:01:01:01-03:01:22, 03:01:24-03:07, 03:09-03:11N, 03:13-03:31, 03:33-03:35, 03:37-03:40, 03:42-03:56, 03:58, 03:60-03:71, 03:73-03:87, 03:90-03:106, 03:109-03:110, 03:112-03:141, 03:143-03:151, 03:153-03:171, 03:174, 25:01:01-25:05, 25:07-25:23, 26:01:01-26:01:20, 26:01:22-26:01:29, 26:02:01w, 26:03:01-26:03:02, 26:05-26:08, 26:10-26:28, 26:29w, 26:30-26:33, 26:35-26:43:02, 26:45-26:48, 26:49w, 26:50-26:72, 26:74-26:77, 26:79-26:90, 26:92-26:94, 30:55, 34:08, 43:01, 66:01:01, 66:04-66:09, 66:10w, 66:11-66:15, 66:17-66:19, 68:71, 74:13
146,7,12 / 80 bp, 115 bp, 200 bp, 240 bp,
470 bp / 1070 bp / A26, A29, A31, A34, / *02:237, 02:309, 03:95, 26:19, 26:22, 29:01:01:01-29:55, 31:03-31:04, 32:42, 34:04, 66:09
156,7,13,14 / 90 bp,
135 bp,
205 bp / 1070 bp / A1, A30 / *01:13, 01:28, 03:43, 03:82, 11:113, 30:01:01-30:04:02, 30:06-30:20, 30:22-30:78N, 31:35
16 / 240 bp,
380 bp / 1070 bp / A2, A24, A31, A32 / *02:237, 03:95, 29:14, 31:01:02-31:77, 32:05, 33:53
17 / 140 bp,
180 bp / 1070 bp / A32 / *01:95, 03:43, 03:82, 24:243, 29:13, 29:39, 31:35, 32:01:01-32:03, 32:05-32:58, 74:07
18 / 200 bp,
390 bp / 1070 bp / A33, A68 / *02:243:01, 29:48, 32:15, 33:01:01-33:01:07, 33:03:01-33:37, 33:39-33:52, 33:54-33:71, 68:29, 74:04
19 / 340 bp,
375 bp / 800 bp / A2, A19, A68, A74 / *01:121, 02:65, 02:407, 68:25, 74:01-74:06, 74:08-74:17
2012 / 210 bp,
240 bp / 800 bp / A2, A210, A25, A68 / *02:34-02:35:03, 02:46, 02:48, 02:56:01-02:56:02, 02:62, 02:70, 02:78, 02:103, 02:129, 25:05, 26:54, 68:01:01:01-68:102
216 / 240 bp,
375 bp / 800 bp / A2, A26, A28, A32, A66, A68, A69 / *02:55, 02:149, 02:243:01, 02:309, 23:57, 24:82, 26:22, 31:41, 32:06, 33:22, 66:06, 66:09, 68:29, 69:01-69:02
226,11 / 85 bp,
240 bp / 800 bp / A2, A36 / *02:34-02:35:03, 02:46, 02:48, 02:56:01-02:56:02, 02:62, 02:70, 02:78, 02:103, 02:129, 31:62, 36:01-36:05
236,12 / 75 bp,
160 bp,
240 bp,
495 bp / 800 bp / A2, A26, A28, A36, A68, A80 / *02:55, 03:41, 03:63, 03:75, 03:88, 11:130, 24:18, 24:204, 24:213, 26:03:01-26:03:02, 26:05-26:06, 26:21, 26:30, 26:78, 33:24, 36:02, 68:05, 68:15, 68:20, 80:01:01:01-80:01:01:02, 80:03
2413 / 360 bp / 1070 bp / A10, A26, A31, A66 / *02:135, 02:309, 03:01:19, 25:13, 26:30, 26:65, 31:04, 34:09, 66:02-66:03, 66:12, 66:16

1Alleles are assigned by the presence of specific PCR product(s). However, the sizes of the specific PCR products may be helpful in the interpretation of HLA-A low resolution SSP typings.

When the primers in a primer mix can give rise to HLA-specific PCR products of more than one length this is indicated if the size difference is more than 20 base pairs. Size differences of 20 base pairs or less are not given. For high resolution SSP kits the respective lengths of the HLA-specific PCR product(s) are given for the alleles amplified by these primer mixes.

Nonspecific amplifications, i.e. a ladder or a smear of bands, may sometimes be seen. GC-rich primers have a higher tendency of giving rise to nonspecific amplifications than other primers.

PCR fragments longer than the control bands may sometimes be observed. Such bands should be disregarded and do not influence the interpretation of the SSP typings.

PCR fragments migrating faster than the control bands, but slower than a 400 bp fragment may be seen in some gel read-outs. Such bands can be disregarded and do not influence the interpretation of the SSP typings.

Some primers may give rise to primer oligomer artifacts. Sometimes this phenomenon is an inherit feature of the primer pair(s) of a primer mix. More often it is due to other factors such as too low amount of DNA in the PCR reactions, taking too long time in setting up the PCR reactions, working at elevated room temperature or using thermal cyclers that are not pre-heated.

2The internal positive control primer pairs amplify segments of the human growth hormone gene. The two different control primer pairs give rise to either an internal positive control band of 1070 base pairs, for most wells, or a band of 800 base pairs, for some wells.

Well number 1 contains the primer pair giving rise to the shorter, 800 bp, internal positive control band in order to help in the correct orientation of the HLA-A low resolution typing.

In addition, wells 2, 4, 6, 8 to 10, 12 and 19 to 23 contain the primer pair giving rise to the shorter, 800 bp, internal positive control band in order to allow kit identification.

In the presence of a specific amplification the intensity of the control band often decreases.

3The serological reactivity of all HLA-A alleles is not known. In this table we use the expert-assigned serological grouping in Tissue Antigens (2009)73:95-170 and the serological grouping of the sequence-defined allele.

4For several HLA Class I alleles 1st and/or 4th exon(s) and beyond, as well as intron nucleotide sequences, are not available. In these instances it is not known whether some of the primers of the SSP sets are completely matched with the target sequences or not. We assume that unknown sequences in these regions are conserved within allelic groups.

5Due to the sharing of sequence motifs between HLA-Class I alleles, primer mix 5 will amplify the C*04:01:03 allele, primer mix 6 will amplify the B*18:27 allele and primer mix 9 will amplify the B*07:81, B*08:52, B*18:67, B*38:41, B*53:05 and B*53:16 alleles

6HLA-specific PCR products shorter than 125 base pairs have a lower intensity and are less sharp than longer PCR products.

7The primer pairs in wells 1, 2, 11, 14 and 15 will in many samples give rise to two or three HLA-specific PCR fragments.

8Primer mix 3 may faintly amplify the A*30:04:01-30:04:02, 30:06, 30:17, 30:29, 30:46 and 30:77 alleles.

9Primer mix 6 may give rise to a lower yield of A*23 alleles than the other A low primer mixes.

10Primer mix 9 may weakly amplify the A*34 alleles.

11Primer mix 22 might faintly amplify most A*11 alleles.

12Primer mixes 6, 10, 14, 20 and 23 may have a tendency of giving rise to primer oligomer formation.

13Primer mixes 6, 15 and 24 may give rise to a lower yield of HLA-specific PCR product than the other HLA-A low primer mixes.

14Primer mix 15 may have tendencies of unspecific amplifications.

‘w’, might be weakly amplified.

1The sequence of the A*0105N has been shown to be identical to A*01:04N.

The A*01:34N allele has been renamed A*01:01:38L

The A*020116 allele has been renamed to A*02:134.

The A*020120 has been renamed to A*02:01:18.

The sequence of the A*02:01:82 allele has been shown to be identical to A*02:01:84.

The sequence of the A*0223 allele has been shown to be identical to A*02:22:01.

The sequence of the A*0298 allele has been shown to be identical to A*02:96.

The A*02:100 allele has never been assigned.

The A*1128 allele has been renamed to A*11:15:02.

The A*11:53 allele has been shown to be identical to the corrected A*11:02:01.

The sequence of the A*2401 allele has been shown to be in error.

The sequence of the A*2412 allele has been shown to be identical to A*24:08.

The A*2416 allele has been renamed to A*31:08.

The A*2465 allele has been renamed to A*24:13:02.

The A*24:211 allele has been renamed to A*24:135:02.

The A*26:44 allele has been renamed to A*26:43:02.

The sequence of the A*3005 allele has been shown to be identical to A*30:04.

The A*3021 allele has been renamed to A*30:11:02.

The sequence of the A*31011 allele has been shown to be identical to A*31:01:02.

The sequence of the A*3302 allele has been shown to be identical to A*33:03:01.

The sequence of the A*33:38 allele has been shown to be identical to A*33:44.

2The serological reactivity of all HLA-A alleles is not known. In this table we use the expert-assigned serological grouping in Tissue Antigens (2009)73:95-170 and the serological grouping of the sequence-defined allele.

3The primer pairs in wells 1, 2, 11, 14 and 15 will in many samples give rise to two or three HLA-specific PCR fragments.

4The A*01:136 and the A*11:94 and A*11:1127 alleles may be distinguished by the different sizes of the specific PCR products generated by primer mixes 1 and 11.

5The A*03:88 and the A*11:130 alleles will give rise to identical amplification patterns. These two alleles can be separated by the respective high resolution SSP primer sets.

6The A*03:152 and the A*24:92 alleles will give rise to identical amplification patterns. These two alleles can be separated by the respective high resolution SSP primer sets.

7The A*23:14:01-23:14:02 and the A*24:24 and 24:71 alleles will give rise to identical amplification patterns. These alleles can be separated by the respective high resolution SSP primer sets.

8The 24:14-24:15, 24:51-24:53, 24:57, 24:64, 24:94, 24:114, 24:138, 24:188, 24:222N, 24:228 and the C*04:01:03 alleles may be distinguished by the different sizes of the specific PCR products generated by primer mix 5.

9The A*31:08 and the A*33:53 alleles will give rise to identical amplification patterns. These alleles can be separated by the respective high resolution SSP primer sets.

10The A*32:01:14, 32:03, 32:38 and the A*74:07 alleles give rise to identical amplification patterns with this lot of the HLA-A low resolution primer set. These alleles can be separated by the respective high resolution primer sets.

‘w’, may be weakly amplified.

Specificities and sizes of the PCR products of the 48 primer mixes used for HLA-B low resolution SSP typing.

Primer Mix / Size of spec. PCR product1 / Size of control band2 / HLA-B
serol.3 / Amplified HLA-B alleles4,5
256,10 / 110 bp / 800 bp / 7, 703, 40, 41, 42, 48, 61 / *07:02:01-07:18:02, 07:20-07:32, 07:34-07:39, 07:41-07:47, 07:49N-07:50, 07:52, 07:54-07:59, 07:61-07:99, 07:101-07:121, 07:123-07:138, 07:140-07:154, 07:156-07:163, 07:166-07:198, 08:79, 15:138, 15:230, 15:241, 37:07, 40:15-40:16, 40:23, 40:32, 40:98, 40:136, 40:158, 40:183, 40:198, 40:231, 41:08, 42:05:01-42:05:02, 44:150, 48:05, 48:08, 48:15, C*02:23, C*04:77
26 / 215 bp / 1070 bp / 8, 44 / *08:01:01-08:05, 08:07-08:25, 08:27-08:49, 08:51-08:61, 08:63-08:64, 08:66-08:84, 08:86N-08:93, 08:95-08:111, 15:142, 15:180, 35:218, 44:49, 44:156, 49:25, 51:68
27 / 140 bp,
235 bp / 1070 bp / 7, 8, 13, 15, 35, 4005, 44, 49, 61, 62, 77 / *07:20, 07:24, 07:60, 07:100, 07:131, 08:21, 08:25, 13:01:01-13:04, 13:06-13:08, 13:10-13:23, 13:25-13:38, 13:40-13:57, 13:59-13:66, 13:68, 13:70, 15:07:01-15:07:02, 15:36w, 15:45, 15:55, 15:68, 15:89w, 15:126, 15:207, 35:05:01-35:05:03, 35:16-35:17, 35:22, 35:30, 35:51, 35:58, 35:72, 35:89, 35:97, 35:113-35:114, 35:125, 35:164, 35:199, 35:232, 40:05, 40:71, 40:174, 44:08w, 44:54, 44:57w, 44:60, 44:106, 44:110, 44:135, 44:158, 46:12, 51:64, 51:148, 53:14, 55:51, 58:18, C*03:05, C*03:25, C*03:27, C*03:143, C*03:167
287,8,16 / 130 bp,
265 bp / 800 bp / 12, 13, 14, 17, 21, 35, 40, 41, 44, 45, 47, 49, 50, 60, 61, 64, 65 / *13:01:01-13:04, 13:06-13:13, 13:15-13:23, 13:25-13:57, 13:59-13:68, 13:70, 14:01:01-14:02:08, 14:03-14:04, 14:07N, 14:09, 14:11-14:12, 14:14-14:36, 15:46, 15:53, 15:106, 15:212, 18:44:01-18:44:02, 35:46-35:47, 35:63, 35:154, 35:207, 35:217, 40:01:01-40:01:29, 40:07, 40:10:01-40:10:02, 40:14:01-40:16, 40:22N-40:23, 40:25, 40:30-40:34, 40:36, 40:38, 40:42-40:43, 40:45, 40:47-40:49, 40:51-40:55, 40:58-40:63, 40:65-40:67, 40:69, 40:72:01-40:73, 40:76-40:77, 40:79-40:81, 40:84, 40:87:01-40:88, 40:92, 40:100-40:102, 40:106, 40:108, 40:110, 40:112-40:114, 40:116-40:118N, 40:121, 40:123-40:126, 40:128-40:130, 40:132, 40:134-40:136, 40:139-40:141, 40:146-40:147, 40:150-40:156, 40:158, 40:160:01-40:160:02, 40:163-40:164, 40:166, 40:168, 40:171-40:172, 40:175, 40:178-40:179, 40:182-40:183, 40:186-40:188, 40:191-40:199, 40:204, 40:207-40:208, 40:212-40:213, 40:215-40:219, 40:221-40:223, 40:227-40:228, 40:231, 40:233-40:240, 40:245, 41:01-41:28, 44:02:01:01-44:03:06, 44:03:08-44:03:15, 44:03:17-44:03:18, 44:04-44:05:04, 44:09-44:39,
44:41:01-44:43:02, 44:45-44:56N, 44:58N-44:80, 44:82, 44:84:01-44:102, 44:104-44:110, 44:112-44:129, 44:132-44:133, 44:135-44:144, 44:146-44:155, 44:157-44:181, 45:01-45:14, 47:01:01:01-47:08, 49:01:01-49:02, 49:04-49:24, 50:01:01-50:02, 50:04-50:06, 50:08-50:20, 50:31-50:33, 53:30, 57:45, 57:51, C*01:30, C*12:87
298 / 185 bp,
235 bp / 800 bp / 7, 16, 17, 27, 2708, 37, 38, 3901, 3902, 39, 64, 67 / *07:02:32, 07:197, 14:01:01-14:01:03, 14:07N-14:08, 14:10, 14:12, 14:14, 14:19, 14:26, 14:32, 27:01-27:05:15, 27:05:17-27:05:19, 27:05:21-27:06, 27:08-27:10, 27:12-27:13, 27:16-27:18, 27:20, 27:23, 27:26-27:27, 27:29, 27:31, 27:35-27:37, 27:39-27:42, 27:44-27:46, 27:48-27:61, 27:64N-27:69, 27:72-27:75, 27:77-27:80, 27:82-27:106, 37:02, 38:01:01-38:02:02, 38:02:04-38:03, 38:07-38:24, 38:26-38:32, 38:34N-38:43, 39:01:01:01-39:01:01:03, 39:01:03-39:01:08, 39:01:10-39:02:01, 39:03, 39:05:01-39:06:02, 39:07-39:09, 39:11, 39:14-39:15, 39:18, 39:19:02, 39:22, 39:24:01-39:39:01, 39:40:01N-39:44, 39:46-39:48, 39:50-39:62, 39:64-39:71, 39:73-39:75, 39:77, 39:79-39:82, 47:04-47:05, 48:21, 48:26, 67:01:01, 67:03
30 / 180 bp / 800 bp / 14, 35, 38, 39, 65 / *07:28, 08:87, 14:02:01-14:02:02, 14:02:04-14:02:05, 14:02:07-14:06:02, 14:09, 14:11, 14:13, 14:15-14:18, 14:20, 14:22-14:23, 14:25, 14:27, 14:29-14:31, 14:33-14:37, 15:77, 15:189, 15:233, 35:26, 38:05, 38:33, 39:04, 44:16, 44:37:01-44:37:02, 44:64:01-44:64:02, 44:91, 44:132, 44:150, 52:26, 57:04,A*23:31, A*24:106, C*07:231, C*16:10
31 / 290 bp / 1070 bp / 15, 22, 62, 63, 71, 72, 75, 76, 77, 46 / *15:01:01:01-15:01:03, 15:01:06-15:02:06, 15:03:03, 15:04-15:08, 15:10:02-15:11:03, 15:11:05-15:15, 15:17:01:01-15:17:02, 15:19-15:21, 15:24:01-15:28, 15:30-15:36, 15:38:01-15:40, 15:42-15:46, 15:48, 15:50, 15:55-15:58, 15:60, 15:63, 15:65-15:66, 15:70-15:71, 15:73, 15:75-15:79N, 15:81-15:89, 15:92, 15:94N, 15:96-15:97, 15:101-15:102, 15:104-15:107, 15:109-15:113, 15:116-15:118, 15:120-15:122, 15:125-15:126, 15:128-15:129, 15:135-15:150, 15:152, 15:154-15:155, 15:157, 15:159-15:160, 15:162-15:172, 15:174-15:175, 15:177-15:179, 15:181N-15:185, 15:187-15:196, 15:199, 15:201-15:209N, 15:211, 15:213-15:219, 15:223-15:225, 15:227-15:228, 15:230-15:234, 15:236-15:237, 15:239-15:241, 15:244-15:251, 15:256-15:262N, 15:264-15:265, 15:267-15:273, 15:276-15:280, 46:01:01-46:40, 46:42, C*01:73, C*07:02:30, C*08:16:02
32 / 165 bp, 220 bp,
285 bp,
330 bp / 1070 bp / 5, 8, 12, 21, 22, 37, 41, 42, 44, 45, 48, 51, 56, 57, 60, 62, 70, 71, 72, 82 / *08:01:01-08:05, 08:08N-08:13, 08:15-08:27, 08:29-08:36, 08:38-08:48, 08:50-08:73, 08:75-08:76, 08:78, 08:80-08:86N, 08:88, 08:90-08:106, 08:109-08:110, 13:46, 15:03:01-15:03:04, 15:09-15:10:02, 15:18:01-15:18:05, 15:23, 15:29, 15:37, 15:46-15:47:02, 15:49, 15:51-15:54, 15:61-15:62, 15:64:01-15:64:02, 15:69, 15:72, 15:74, 15:80, 15:83, 15:90-15:91, 15:93,
15:98-15:99, 15:103, 15:106, 15:108, 15:114-15:115, 15:119, 15:123-15:124, 15:127, 15:131-15:134, 15:143, 15:151, 15:153, 15:156, 15:158, 15:161, 15:173, 15:176, 15:186, 15:197-15:198, 15:200, 15:210, 15:212, 15:220-15:221, 15:226N, 15:229, 15:235, 15:238, 15:242-15:243, 15:252-15:253, 15:255, 15:259, 15:263, 15:266, 15:274-15:275, 15:281-15:282, 35:87, 37:12, 38:30, 40:12, 40:136, 40:231, 41:01-41:03:02, 41:05-41:09, 41:11-41:17, 41:19-41:28, 42:01:01-42:02, 42:04-42:08, 42:10-42:15, 42:17-42:19, 44:14-44:15, 44:18, 44:20, 44:62, 44:100, 44:166, 45:01-45:14, 51:08, 51:20, 51:36, 51:44N, 51:97, 51:141, 51:153, 52:19, 55:20, 55:56, 56:13, 57:09, 82:01-82:03, C*07:46
338,9,11 / 165 bp, 190 bp, 390 bp / 1070 bp / 5, 17, 21, 35, 51, 5102, 5103, 52, 56, 62, 63, 70, 78 / *13:62, 15:01:02, 15:09, 15:16:01-15:17:02, 15:67, 15:95, 15:162, 15:168, 15:177, 15:196, 15:208, 15:216, 15:222, 15:230, 15:243, 15:254, 15:268, 15:273, 15:277-15:278, 35:01:10, 35:04:02, 40:26, 40:28, 44:62, 49:18, 50:14, 51:01:01-51:01:03, 51:01:05-51:02:03, 51:02:05-51:09:02, 51:11N-51:12, 51:13:02-51:14, 51:16-51:23, 51:24:03-51:24:04, 51:26-51:33, 51:37-51:41N, 51:43-51:44N, 51:46, 51:48-51:61:02, 51:63-51:80, 51:82-51:91, 51:94-51:98N, 51:100-51:130, 51:132-51:142, 51:145-51:147, 51:149N-51:154, 52:01:01:01-52:01:04, 52:01:06-52:01:14, 52:01:16-52:13, 52:15-52:24, 52:26-52:31, 53:28, 56:05:01-56:06, 56:21, 58:08:01-58:08:02, 78:01:01-78:07
34 / 180 bp / 800 bp / 13, 22, 55, 60 / *07:78, 13:01:01-13:02:15, 13:07N-13:09, 13:11, 13:14-13:20, 13:22:01-13:23, 13:25, 13:27-13:34, 13:36-13:45, 13:47, 13:49N-13:50, 13:52, 13:54-13:58, 13:60-13:61, 13:63N-13:68, 13:70, 40:48, 45:10, 49:07, 50:31, 54:26, 55:09, 55:22, 55:24
356 / 100 bp,
195 bp / 1070 bp / 8, 12, 27, 38, 39, 3902, 40, 44, 45, 48, 60, 70, 71, 72, 82 / *07:27, 07:50, 08:04, 08:17, 08:54, 08:110, 15:03:01-15:03:04, 15:47:01-15:47:02, 15:49, 15:54, 15:61-15:62, 15:64:01-15:64:02, 15:68-15:69, 15:91, 15:98, 15:103, 15:123, 15:127, 15:131-15:132, 15:151, 15:156, 15:158, 15:173, 15:210, 15:220, 15:235, 15:242, 15:253, 15:259, 15:266, 15:274, 15:281-15:282, 18:37, 27:18, 27:29, 37:28, 38:03, 39:02:01-39:02:02, 39:08, 39:13:01-39:13:02, 39:23, 39:39:01-39:39:02, 39:49, 40:12, 40:46, 40:93, 42:11, 44:10, 44:15, 44:18, 44:40, 44:44, 44:130, 44:140, 44:156, 45:01, 45:05-45:07, 45:11-45:14, 48:01:01-48:05, 48:07-48:30, 49:20, 50:02, 52:16, 55:18, 58:44, 82:01-82:03
36 / 280 bp / 1070 bp / 5, 7, 8, 13, 15, 16, 17, 18, 22, 35, 40, 42, 44, 46, 48, 49, 51, 53, 55, 56, 57, 58, 60, 61, 62, 63, 70, 71, 72, 75, 76, 77, 78 / *07:09, 07:11, 07:17, 07:162, 08:28, 08:35, 08:37, 08:89, 08:107, 13:04, 13:10, 13:26, 15:01:01:01-15:01:04, 15:01:06-15:08, 15:11:01-15:16:03, 15:18:01-15:21, 15:23-15:29, 15:31-15:36, 15:38:01-15:40, 15:43-15:44, 15:46-15:47:02, 15:49-15:57, 15:60-15:62, 15:64:01-15:72, 15:74-15:76, 15:78:01-15:82, 15:84-15:85,
15:87-15:89, 15:91-15:98, 15:101-15:129, 15:131-15:132, 15:134-15:136, 15:138-15:149N, 15:151-15:161, 15:163-15:167, 15:169-15:176, 15:178-15:187, 15:189-15:195, 15:197-15:207, 15:209N-15:215, 15:217-15:223, 15:225-15:228, 15:231-15:232, 15:234-15:242, 15:244-15:247, 15:249-15:251, 15:254-15:268, 15:270-15:272N, 15:274-15:282, 18:01:01:01-18:15, 18:17N-18:25, 18:27-18:40, 18:42-18:60, 18:62-18:91, 27:41, 35:01:01:01-35:01:38, 35:05:01-35:05:03, 35:07-35:08:06, 35:10-35:11:03, 35:14:01-35:17, 35:19-35:21, 35:23-35:30, 35:32:01-35:32:02, 35:35, 35:37, 35:40N-35:43:01, 35:45-35:54, 35:57-35:58, 35:61:01-35:64:02, 35:66-35:69, 35:71-35:72, 35:76-35:80, 35:82, 35:86, 35:89-35:94, 35:97, 35:99-35:105, 35:107-35:108:02, 35:110-35:126, 35:130N-35:135, 35:137-35:148, 35:158-35:159, 35:161, 35:165N-35:166, 35:168, 35:170-35:171, 35:173N-35:178, 35:180, 35:185-35:192, 35:194, 35:196-35:197, 35:200, 35:202-35:203, 35:206-35:210, 35:212, 35:215-35:219, 35:222, 35:224-35:229, 35:232, 37:35, 39:07, 39:43, 40:03, 40:20, 40:38, 40:52, 40:59-40:60, 40:105, 40:158-40:159, 40:222, 41:24, 42:09, 44:17, 44:43:01-44:43:02, 44:144, 45:09, 46:01:01-46:10, 46:12-46:17, 46:19-46:40, 46:42, 48:02:01-48:02:03, 48:14, 48:23, 48:25, 49:04-49:05, 50:33, 51:37, 51:45, 51:63, 51:97, 53:01:01-53:03, 53:05-53:06, 53:08:01-53:18, 53:20-53:30, 54:06, 54:09, 54:14, 55:14, 55:23, 55:32, 56:03, 56:09, 56:18, 56:31-56:32, 57:01:01-57:01:15, 57:06, 57:08, 57:10-57:11, 57:13-57:16, 57:18-57:27, 57:29-57:31, 57:33-57:38, 57:40-57:41, 57:43-57:45, 57:47-57:56, 57:58-57:62, 57:64-57:65, 58:01:01-58:02, 58:04-58:07, 58:09-58:19, 58:21-58:26, 58:29, 58:31N-58:33, 58:35-58:46
37 / 195 bp / 1070 bp / 27, 35, 37, 44, 58, 76 / *15:12, 15:14, 15:19, 15:91, 15:131, 15:161, 15:270, 18:54, 18:56, 35:45, 35:71, 37:01:01-37:09, 37:12-37:13, 37:15-37:21, 37:23-37:28, 37:30N-37:33N, 37:35-37:36, 38:17, 44:17, 44:43:01-44:43:02, 44:144, 45:09, 46:17, 53:22, 58:07, A*24:174
386 / 105 bp, 395 bp, 435 bp / 1070 bp / 5, 7, 15, 41, 42, 62, 63, 70, 71, 75, 77 / *07:04, 07:25, 07:146, 15:09-15:10:02, 15:13:01-15:13:02, 15:16:01-15:18:05, 15:21, 15:23-15:24:02, 15:37, 15:44, 15:51-15:52, 15:66-15:67, 15:72, 15:80, 15:87, 15:90, 15:93, 15:95, 15:99, 15:108, 15:114-15:115, 15:119, 15:124, 15:133-15:134, 15:153, 15:157, 15:161-15:162, 15:168, 15:176-15:177, 15:186, 15:189, 15:196-15:198, 15:200, 15:208, 15:216, 15:221-15:222, 15:226N, 15:229-15:230, 15:238, 15:243, 15:252, 15:254, 15:263, 15:268, 15:273, 15:275, 40:136, 40:231, 41:08, 42:05:01-42:05:02
396 / 115 bp,
150 bp / 1070 bp / 18 / *18:01:01:01-18:15, 18:17N-18:28, 18:30-18:71, 18:73-18:91, 48:28
406 / 80 bp / 1070 bp / 7, 27, 2708, 44, 60 / *07:73, 08:88, 08:102, 27:01-27:21, 27:23-27:51,
27:53-27:66N, 27:68-27:74, 27:76-27:106, 38:22, 40:46, 40:93, 44:40, 44:44, 44:130, 49:26
41 / 150 bp / 800 bp / 12, 13, 17, 18, 22, 27, 35, 37, 39, 44, 45, 47, 48, 51, 53, 56, 57, 58, 62, 70, 75, 77, 78 / *08:49, 13:01:01-13:01:06, 13:06-13:07N, 13:12:01-13:13, 13:17, 13:20, 13:22:01-13:23, 13:25-13:26, 13:28-13:29, 13:36, 13:39, 13:43, 13:50-13:52, 13:57, 13:60-13:61, 13:63N, 14:10, 15:02:01-15:02:06, 15:13:01-15:13:02, 15:20-15:21, 15:25:01-15:25:03, 15:36, 15:44, 15:62, 15:77, 15:80, 15:85, 15:88-15:89, 15:106, 15:112, 15:121, 15:139, 15:144, 15:154, 15:165, 15:170, 15:194, 15:204, 15:213-15:214, 15:223, 15:240, 15:250, 15:265, 15:271, 18:22, 18:69, 27:19, 27:30, 35:01:01:01-35:04:03, 35:06-35:08:06, 35:10-35:17, 35:19-35:21, 35:23-35:30, 35:33-35:36, 35:38-35:42:02, 35:45-35:50, 35:52, 35:54-35:57, 35:59, 35:61:01-35:63, 35:65Q, 35:69-35:71, 35:74, 35:76-35:78, 35:80-35:85, 35:90-35:96, 35:98, 35:100-35:101:02, 35:103-35:113, 35:115-35:116, 35:120-35:126, 35:128-35:134N, 35:136-35:150, 35:152-35:173N, 35:175-35:184, 35:186-35:198, 35:200-35:204, 35:206-35:229, 35:231, 35:233, 37:01:01-37:01:07, 37:01:09, 37:03N-37:06, 37:08, 37:10-37:11, 37:13-37:37, 38:20, 39:42, 40:28, 44:02:01:01-44:14, 44:16-44:17, 44:19N, 44:21-44:30, 44:32-44:40, 44:42-44:46, 44:48-44:52N, 44:55-44:64:02, 44:66-44:98, 44:101-44:105, 44:107-44:134, 44:136-44:137, 44:139-44:157, 44:159-44:165, 44:167-44:181, 46:33, 48:02:01-48:02:03, 48:25, 50:17, 51:04, 51:42, 51:46, 51:56:01-51:56:02, 51:139, 53:01:01-53:13, 53:15-53:31, 55:14, 56:09, 56:11-56:12, 57:01:01-57:01:04, 57:01:06-57:10, 57:12, 57:14-57:20, 57:22-57:30, 57:32-57:46, 57:48-57:65, 58:01:01-58:01:02, 58:01:04-58:01:12, 58:04-58:05, 58:09-58:15, 58:17N, 58:19, 58:21-58:24, 58:28-58:29, 58:31N-58:37, 58:39N-58:42, 58:44-58:45, 83:01, C*03:102
42 / 140 bp / 1070 bp / 8, 18, 22, 35, 39, 78 / *07:65, 07:134, 08:32, 15:202, 15:239, 18:01:01:01-18:08, 18:10-18:15, 18:17N-18:36, 18:38-18:47, 18:50-18:53, 18:55-18:65, 18:68-18:78, 18:80-18:91, 35:01:01:01-35:18, 35:20:01-35:24:02, 35:28-35:45, 35:48, 35:50-35:55, 35:57-35:62, 35:64:01-35:72, 35:74-35:153, 35:155-35:197, 35:199-35:202, 35:204-35:206, 35:208-35:209, 35:211-35:216N, 35:218-35:229, 35:231-35:233, 37:11, 37:37, 39:19:01-39:19:02, 40:185, 40:209, 46:40, 56:06, 78:01:01-78:05, 78:07
436,10 / 60 bp,
245 bp, 400 bp / 1070 bp / 5, 15, 44, 48, 60, 62, 63, 70, 71, 72, 75, 76, 77, 82 / *15:01:01:01-15:01:04, 15:01:06-15:21, 15:23-15:40, 15:42-15:58, 15:60-15:67, 15:69-15:99, 15:102-15:129, 15:131-15:179, 15:181N-15:235, 15:237-15:254, 15:256-15:282, 40:12, 82:01-82:03,C*03:179
4410 / 210 bp / 800 bp / 27, 37 / *07:27, 08:49, 18:54, 37:01:01-37:37, 38:17,C*07:302
45 / 170 bp / 800 bp / 16, 35, 38, 39, 3901, 3902, 67, 72 / *08:55, 15:69, 15:186, 35:35, 38:01:01-38:09, 38:11-38:21, 38:23-38:29, 38:31-38:35, 38:38-38:43, 39:01:01:01-39:01:01:03, 39:01:03-39:20, 39:22-39:24:02, 39:26-39:29, 39:31, 39:35, 39:37-39:42, 39:44-39:46, 39:48-39:49, 39:51-39:73, 39:75-39:81, 39:83, 40:184, 51:101, 58:20, 67:01:01-67:03, C*15:51
466 / 110 bp / 1070 bp / 38 / *38:01:01-38:02:02, 38:02:04-38:03, 38:05, 38:08-38:24, 38:26-38:34N, 38:36-38:43, 49:26, 52:01:10
47 / 395 bp / 1070 bp / 14, 16, 38, 39, 3901, 64, 65 / *07:69, 07:85, 07:180, 08:65, 14:01:01-14:20, 14:22-14:37, 38:01:01-38:02:05, 38:04-38:05, 38:08-38:25, 38:27-38:43, 39:01:01:01-39:01:01:03, 39:01:03-39:01:16, 39:03-39:07, 39:09, 39:11-39:12, 39:14-39:15, 39:18-39:19:02, 39:22, 39:24:01-39:38Q, 39:40:01N-39:48, 39:50-39:57, 39:59-39:62, 39:64-39:82, 49:26
48 / 160 bp,
425 bp / 1070 bp / 7, 703, 8, 14, 15, 16, 21, 39, 3901, 3902, 40, 41, 42, 45, 48, 50, 60, 61, 64, 65, 67, 70, 73, 81 / *07:02:01-07:26, 07:28-07:35, 07:37, 07:39-07:64, 07:66-07:80, 07:82-07:148, 07:150-07:178, 07:181N-07:191, 07:193-07:194, 07:196-07:198, 08:01:01-08:01:21, 08:04-08:05, 08:07-08:35, 08:37-08:51, 08:53:01-08:77, 08:79-08:111, 14:01:01-14:02:04, 14:02:06-14:02:07, 14:02:09-14:29, 14:31-14:37, 27:14, 27:81, 39:01:01:01-39:01:01:03, 39:01:03-39:20, 39:22-39:24:01, 39:25N-39:83, 40:01:01-40:01:29, 40:06:01:01-40:06:04, 40:06:06-40:07, 40:09?, 40:11:01?-40:11:02?, 40:14:01?-40:15?, 40:16, 40:18?, 40:21?-40:22N?, 40:23, 40:24?-40:38?, 40:42?-40:43?, 40:44, 40:45?-40:46?, 40:48?-40:50?, 40:51, 40:52?, 40:53, 40:54?-40:58?, 40:59-40:60, 40:61?-40:65?, 40:66, 40:67?-40:69?, 40:70, 40:71?, 40:72:01-40:73, 40:74?, 40:75, 40:77, 40:78?, 40:79, 40:80?-40:82?, 40:83, 40:84?-40:85?, 40:86, 40:87:01?-40:88?, 40:90?-40:92?, 40:93, 40:95?, 40:96, 40:98?-40:102?, 40:103, 40:104?-40:108?, 40:109-40:110, 40:111?-40:116?, 40:118N?-40:121?, 40:123?, 40:124:01, 40:124:02?-40:126?, 40:127, 40:128?-40:130?, 40:131, 40:132?-40:134?, 40:136?-40:137?, 40:138-40:140, 40:141?, 40:145?, 40:146-40:148, 40:149?, 40:150, 40:151?, 40:152-40:153, 40:154?, 40:155N, 40:156?, 40:158?, 40:159, 40:160:01?-40:160:02?, 40:161-40:162, 40:163?-40:164?, 40:165, 40:166?, 40:167, 40:168?-40:171?, 40:172, 40:173?-40:176?, 40:177, 40:178?-40:185?, 40:186, 40:187?, 40:189?, 40:190, 40:191?-40:200?, 40:202?-40:213?, 40:215?-40:228?, 40:230, 40:231?-40:240?, 40:244, 40:245?-40:246?, 41:01-41:28, 42:01:01-42:02, 42:04-42:20, 45:01-45:14, 48:01:01-48:17, 48:19-48:31, 50:01:01-50:02, 50:04-50:20, 50:31-50:33, 51:01:09, 51:01:21, 51:01:25, 51:10, 51:24:02-51:24:04, 54:11, 56:20:02, 67:01:01-67:03, 73:01-73:02, 81:02
4912 / 180 bp / 1070 bp / 15, 40, 44, 48, 60, 62, 71 / *15:116, 15:124, 40:01:01-40:01:06, 40:01:08-40:01:17, 40:01:19-40:01:29, 40:07, 40:10:01-40:10:02, 40:12, 40:21-40:23, 40:25, 40:30, 40:33-40:34, 40:36, 40:38, 40:42-40:43, 40:46-40:49, 40:51-40:52, 40:54-40:55, 40:59-40:63, 40:65-40:67, 40:69, 40:73-40:74, 40:76, 40:79, 40:81, 40:84, 40:87:01-40:88, 40:92, 40:100-40:102, 40:106, 40:108, 40:112-40:114, 40:116-40:118N, 40:123-40:126, 40:128, 40:130, 40:132, 40:134-40:135, 40:137-40:141, 40:146-40:147, 40:149-40:156, 40:160:01-40:160:02, 40:163, 40:166, 40:168, 40:170-40:172, 40:175, 40:178-40:179, 40:182-40:183, 40:185-40:188, 40:191, 40:193-40:196, 40:198-40:199, 40:204, 40:207-40:208, 40:210, 40:212-40:213, 40:215-40:218, 40:221-40:223, 40:227-40:228, 40:231, 40:234-40:236, 40:238-40:240, 40:245, 44:31, 44:169, 46:06, 48:03:01-48:03:02, 48:17, 48:23, 57:59, C*03:129, C*17:16
5010,17 / 290 bp, 465 bp / 1070 bp / 21, 4005, 41, 50, 60, 61 / *13:02:06, 13:26, 15:16:03, 15:20, 15:85, 15:194, 18:48, 35:46, 35:207, 40:02:01-40:06:07, 40:08, 40:09?, 40:10:02?-40:11:02?, 40:13, 40:14:01?-40:15?, 40:18?-40:19?, 40:20, 40:22N?, 40:24?-40:38?, 40:39, 40:42?-40:45?, 40:47?-40:50?, 40:52?-40:58?, 40:61?-40:65?, 40:67?-40:69?, 40:70, 40:71?, 40:72:02?, 40:74?-40:76?, 40:78?, 40:80?-40:82?, 40:84?-40:88?, 40:89, 40:90?-40:92?, 40:94, 40:95?-40:96?, 40:97, 40:98?-40:121?, 40:122, 40:123?, 40:124:02?-40:136?, 40:141?, 40:142N-40:144N, 40:145?, 40:148?-40:149?, 40:151?, 40:154?, 40:156?-40:169?, 40:171?, 40:173?-40:176?, 40:178?-40:184?, 40:187?-40:208?, 40:210?-40:213?, 40:214, 40:216N?-40:228?, 40:229, 40:230?-40:240?, 40:244?-40:246?, 46:01:07, 46:33, 47:01:01:01-47:08, 57:05, 58:36, C*01:73
516 / 105 bp / 1070 bp / 7, 8, 22, 41, 42 / *07:04, 07:25, 07:146, 08:01:01-08:05, 08:07-08:12:03, 08:14-08:19N, 08:21-08:24, 08:26-08:39, 08:41-08:54, 08:56:01-08:69, 08:71-08:78, 08:80-08:102, 08:104-08:111, 35:87, 40:136, 40:231, 41:01-41:08, 41:10-41:28, 42:01:01-42:02, 42:04-42:07, 42:09-42:18, 42:20, 44:166, 55:20
52 / 325 bp / 800 bp / 7, 22, 27, 2708, 35, 42, 45, 46, 54, 55, 56, 67, 73, 78, 81, 82 / *07:02:01-07:02:39, 07:04-07:07, 07:09-07:15, 07:17-07:26, 07:28-07:31, 07:33:01-07:36, 07:39-07:46, 07:47w, 07:48-07:49N, 07:51-07:68:03, 07:70-07:84, 07:86-07:179, 07:181N-07:198, 14:21, 15:76, 15:101, 15:255, 27:01-27:11, 27:13-27:15, 27:17, 27:19-27:21, 27:24-27:28, 27:30-27:38, 27:40-27:58, 27:60-27:76, 27:78-27:91, 27:93-27:106, 35:76, 35:186, 35:229, 38:26, 40:166, 42:01:01-42:02, 42:04-42:06, 42:08-42:10, 42:12-42:20, 44:90, 44:97, 45:06, 46:01:01-46:01:09, 46:02w, 46:03-46:40, 46:42, 54:01:01-54:29, 55:01:01-55:05, 55:07-55:17, 55:19-55:59, 56:01:01-56:16, 56:18-56:22, 56:24-56:32, 56:34-56:39, 56:41, 67:01:01-67:03, 73:01-73:02, 81:01-81:06, 82:01-82:03, 83:01
536,8,10 / 80 bp, 115 bp, 160 bp, 195 bp, 225 bp, 260 bp / 1070 bp / 35, 37, 44, 47, 57 / *08:49, 08:60, 08:76, 08:87, 35:38, 35:45, 35:71, 35:115, 35:169, 37:01:01-37:01:07, 37:01:09, 37:03N-37:06, 37:08, 37:10-37:37, 40:132, 40:178, 40:231, 42:13, 44:02:01:01-44:14, 44:16-44:17, 44:19N-44:36,
44:38-44:63, 44:65-44:134, 44:136-44:149N, 44:151-44:157, 44:159-44:165, 44:167-44:181, 51:42, 53:22, 57:07, 57:09, 57:24, 83:01, C*15:25
54 / 215 bp / 800 bp / 5, 8, 12, 17, 21, 22, 41, 42, 44, 45, 51, 5102, 5103, 52, 53, 56, 62 / *08:09, 08:84, 13:46, 15:83, 41:01, 41:05-41:07, 41:09, 41:12, 41:14, 41:16-41:17, 41:20-41:22, 41:25-41:26, 41:28, 42:04, 44:06, 44:15, 44:18, 44:20, 44:100, 45:01-45:14, 51:01:01, 51:01:03-51:01:08, 51:01:10-51:01:39, 51:01:41-51:02:01, 51:02:03-51:04, 51:06:01-51:07:01, 51:08-51:09:01, 51:10-51:14, 51:16-51:24:04, 51:26-51:46, 51:48-51:53, 51:55-51:77, 51:79-51:88, 51:90-51:92, 51:94-51:102, 51:104-51:115, 51:117-51:118N, 51:120-51:131, 51:133-51:143, 51:145-51:147, 51:149N-51:154, 52:01:02, 52:01:04, 52:01:09, 52:02:01-52:03, 52:06:01-52:06:02, 52:09, 52:19, 52:21, 53:06, 55:20, 55:56, 56:13, 58:08:01
55 / 130 bp,
270 bp / 800 bp / 17, 18, 22, 35, 37, 46, 51, 53, 62, 78 / *14:10, 15:57w, 18:22, 18:69, 35:21, 35:24:01-35:24:02, 35:81, 35:96, 35:109, 35:157, 35:188, 35:190, 35:233, 37:04:01-37:04:02, 40:28, 46:01:01-46:39, 46:42, 51:04, 51:46, 51:56:01-51:56:02, 51:139, 53:02, 53:06, 53:28, 57:14, 58:09, C*01:73, C*15:39
566,13 / 90 bp,
410 bp / 1070 bp / 15, 22, 27, 35, 47, 54, 55, 56, 78, 81, 82 / *07:65, 27:01w, 27:02:01-27:11, 27:13-27:15, 27:17, 27:19-27:21, 27:24-27:28, 27:30-27:38, 27:40-27:58, 27:60-27:76, 27:78-27:91, 27:93-27:106, 35:76, 35:186, 35:229, 44:90, 44:97, 47:01:01:01-47:03, 47:06-47:08, 54:01:01-54:29, 55:01:01-55:05, 55:07-55:17, 55:19-55:59, 56:01:01-56:16, 56:18-56:22, 56:24-56:32, 56:34-56:39, 56:41, 81:01, 82:01-82:03, 83:01
576 / 90 bp,
175 bp / 1070 bp / 15, 27, 35, 48, 60, 62, 75, 77, 81 / *07:174, 13:36, 15:02:01-15:02:06, 15:08, 15:11:01-15:11:05, 15:13:01-15:13:02, 15:15, 15:21, 15:31, 15:44, 15:55, 15:76w, 15:88-15:89, 15:112, 15:121, 15:139, 15:144, 15:148, 15:170, 15:189, 15:191, 15:194, 15:209N, 15:213-15:215, 15:223, 15:265, 27:24, 35:46, 40:31, 40:45, 40:80, 48:01:01-48:01:04, 48:04, 48:06-48:07, 48:09, 48:11, 48:15-48:16, 48:18-48:20, 48:22, 48:24, 48:27-48:31, 56:39w, 81:01-81:06
58 / 145 bp,
430 bp / 1070 bp / 44, 49, 59, 61 / *40:13, 40:19, 40:109, 40:117, 44:18, 44:25, 44:50, 44:95, 49:01:01-49:01:04, 49:04-49:25, 51:112, 53:30, 54:12, 56:21, 57:45, 57:51, 59:01:01:01-59:06
596 / 120 bp,
210 bp / 800 bp / 5, 13, 15, 17, 22, 35, 45, 49, 50, 51, 5102, 5103, 52, 55, 56, 62, 63, 78 / *07:78, 07:84, 13:16, 13:31w, 13:48, 13:62, 14:37, 15:04w, 15:16:01-15:16:03, 15:42, 15:67, 15:95, 15:137w, 15:254, 40:95, 40:148, 40:161, 46:32w, 49:01:01, 49:01:03-49:10, 49:12-49:26, 50:01:01-50:02, 50:04-50:11, 50:13-50:16, 50:18-50:20, 50:31-50:33, 51:01:01-51:03, 51:05, 51:07:01-51:09:02, 51:11N-51:14, 51:16-51:24:04, 51:26-51:41N, 51:43-51:44N, 51:48-51:55, 51:57-51:58, 51:60-51:61:02, 51:63, 51:65-51:80, 51:82-51:134, 51:136-51:138, 51:140-51:147, 51:149N-51:154, 52:01:01:01-52:31, 54:20, 55:01:01-55:01:06, 55:01:08-55:01:11, 55:03, 55:05, 55:09, 55:11, 55:15, 55:17, 55:21w,
55:24-55:25, 55:28-55:29, 55:31, 55:33, 55:36, 55:38, 55:40, 55:44-55:45, 55:52-55:55N, 55:58-55:59, 56:05:01-56:06, 56:21, 56:25, 56:37, 58:08:01-58:08:02, 78:01:01-78:07
60 / 430 bp / 1070 bp / 5, 17, 27, 44, 51, 5102, 5103, 52, 53, 57, 58, 61 / *18:67, 27:02:01-27:02:02, 27:30, 27:53, 27:57, 27:62, 27:65N, 27:75, 27:77, 27:83, 27:95, 27:102, 37:34, 40:13, 40:19, 40:109, 40:117, 44:06, 44:25, 44:50, 44:95, 51:01:01-51:01:39, 51:01:41-51:24:04, 51:26-51:46, 51:48-51:53, 51:55-51:77, 51:79-51:154, 52:01:01:01-52:19, 52:21-52:31, 53:01:01-53:02, 53:04-53:08:02, 53:10, 53:14-53:30, 57:01:01-57:11, 57:13-57:65, 58:01:01-58:02, 58:04-58:16:02, 58:18-58:29, 58:31N-58:46
61 / 145 bp / 1070 bp / 12, 21, 35, 40, 4005, 41, 45, 47, 50, 60, 61 / *07:133, 15:46, 15:53, 15:106, 15:143, 15:212, 18:48, 35:19, 35:47, 35:63, 35:154, 35:217, 39:69, 40:01:01-40:11:02, 40:14:01-40:16, 40:18, 40:20, 40:22N-40:40, 40:42-40:45, 40:48-40:75, 40:77-40:92, 40:94-40:95, 40:97-40:108, 40:111-40:116, 40:118N-40:136, 40:138-40:156, 40:158-40:184, 40:186-40:187, 40:189-40:200, 40:202-40:207, 40:210-40:240, 40:244-40:246, 41:01-41:04, 41:05?, 41:06-41:25, 41:27-41:28, 44:09, 44:46, 44:75, 44:90, 44:129, 44:131, 45:01-45:14, 47:02, 47:03w, 50:01:01-50:02, 50:04-50:05, 50:07-50:20, 50:31-50:33
62 / 300 bp / 1070 bp / 7, 12, 13, 15, 17, 18, 21, 27, 2708, 35, 37, 38, 39, 3902, 40, 4005, 41, 44, 45, 47, 48, 49, 50, 52, 60, 61, 62, 70, 72, 76 / *07:54, 07:123, 08:17, 08:38, 08:54, 08:101, 13:01:01-13:04, 13:06, 13:08-13:23, 13:25-13:35, 13:37-13:68, 13:70, 15:01:01:01-15:01:04, 15:01:06-15:01:18, 15:01:20-15:01:33, 15:03:01-15:07:02, 15:12, 15:14, 15:19-15:20, 15:24:01-15:27:03, 15:30, 15:32-15:36, 15:38:01-15:40, 15:42-15:43, 15:45-15:50, 15:53-15:54, 15:56-15:58, 15:60-15:63, 15:65-15:66, 15:68-15:71, 15:73-15:75, 15:77-15:79N, 15:81-15:87, 15:91-15:92, 15:94N, 15:96-15:98, 15:101-15:107, 15:109-15:111N, 15:113, 15:116-15:118, 15:122-15:123, 15:125-15:129, 15:131-15:132, 15:135-15:138, 15:140-15:142, 15:145-15:147, 15:150-15:152, 15:154-15:160, 15:163-15:167, 15:169, 15:171-15:175, 15:178-15:179, 15:181N-15:185, 15:187-15:188, 15:190N, 15:192-15:193, 15:195, 15:199, 15:201-15:207, 15:210-15:212, 15:217-15:220, 15:224-15:225, 15:227-15:228, 15:231-15:237, 15:239-15:245Q, 15:247-15:251, 15:253, 15:255-15:262N, 15:264, 15:266-15:267, 15:269-15:272N, 15:274, 15:276-15:282, 18:12, 27:01-27:21, 27:24-27:91, 27:93-27:106, 35:10, 35:13, 35:16, 35:28, 35:63, 35:69, 35:80, 35:207, 35:226, 37:01:01-37:07, 37:09-37:37, 38:03-38:04, 38:25, 39:02:01-39:02:02, 39:08, 39:13:01-39:13:02, 39:22-39:23, 39:49, 40:01:01-40:07, 40:09-40:16, 40:18-40:24, 40:26-40:40, 40:42-40:67, 40:70-40:105, 40:107-40:165, 40:167-40:231, 40:233-40:240, 40:244-40:246, 41:01-41:28, 42:11, 44:02:01:01-44:02:21, 44:02:23-44:05:04, 44:07-44:11, 44:13-44:170, 44:172-44:181, 45:01-45:14, 47:01:01:01-47:08, 48:01:01-48:05, 48:07-48:15, 48:17-48:31, 49:01:01-49:25, 50:01:01-50:02, 50:04-50:14, 50:16-50:20, 50:31-50:33, 52:01:01:01-52:31, 53:17:01-53:17:02, 53:28, 55:18, 55:34, 55:40, 78:05-78:06
638,14 / 160 bp,
330 bp / 1070 bp / 22, 44, 45, 47, 54 / *08:87, 44:02:01:01-44:02:29, 44:06, 44:08-44:09, 44:11-44:12, 44:16, 44:19N-44:24, 44:27:01-44:27:02, 44:33-44:34:02, 44:41:01-44:41:02, 44:44, 44:48-44:49, 44:52N-44:53, 44:55, 44:59:01-44:59:02, 44:63, 44:66-44:68, 44:71-44:74, 44:80, 44:83-44:84:01, 44:86-44:87, 44:89-44:91, 44:93, 44:95, 44:97, 44:99-44:102, 44:104, 44:106, 44:112-44:113, 44:116, 44:118-44:119, 44:121, 44:126:01-44:127, 44:131-44:132, 44:137-44:138Q, 44:142, 44:145, 44:148-44:149N, 44:151-44:152, 44:162, 44:168-44:173, 44:176-44:177, 44:179, 54:01:01, 54:01:03-54:30, 83:01
64 / 180 bp,
210 bp / 1070 bp / 13, 15, 18, 35, 39, 40, 54, 55, 56, 59, 78 / *07:65, 13:06, 13:53, 14:37, 15:42, 15:86, 15:224, 18:04, 35:08:05, 35:42:02, 35:60, 35:185, 39:17, 39:63, 40:01:11, 40:58, 45:08, 46:18, 46:40, 54:01:01-54:02, 54:05N, 54:07-54:08N, 54:10, 54:12-54:13, 54:16-54:25, 54:27-54:30, 55:01:01-55:03, 55:05, 55:07, 55:10-55:12, 55:15-55:16, 55:18-55:19, 55:21, 55:25-55:26, 55:29-55:31, 55:33-55:45, 55:47-55:48, 55:50, 55:52-55:57, 55:59, 56:05:01, 56:06, 56:10, 56:23, 59:01:01:01-59:01:01:02, 59:05-59:06, 78:01:01-78:01:02, 78:02:02-78:03, 78:07, C*15:02:04
65 / 180 bp / 1070 bp / 12, 15, 21, 22, 44, 45, 49, 50, 51, 56, 61, 62, 82 / *13:03, 13:48, 15:73, 40:71, 44:10, 44:15, 44:18, 44:140, 45:01, 45:04-45:07, 45:11-45:14, 46:11, 49:01:01-49:03, 49:06, 49:08-49:17, 49:19N-49:26, 50:01:01-50:02, 50:04-50:08, 50:10-50:13, 50:15-50:19, 50:32, 51:15, 51:62, 51:106, 52:25, 54:03, 56:01:01-56:01:04, 56:01:06-56:02, 56:04, 56:07-56:08, 56:13-56:14, 56:16-56:17, 56:20:01-56:20:02, 56:24-56:30, 56:33-56:39, 56:41-56:42, 59:04, 82:01-82:03, C*03:19, C*03:102
666 / 90 bp,
240 bp / 800 bp / 57 / *55:14, 57:01:01-57:44, 57:46-57:50, 57:52-57:65, 58:36, C*06:72
676,13 / 90 bp / 1070 bp / 5, 13, 15, 17, 39, 55, 56, 58, 60, 61, 62, 71 / *07:182N, 13:11, 15:18:03, 15:73, 15:224, 39:17, 40:48, 40:71, 51:62, 56:02, 56:04, 56:10, 58:01:01-58:02, 58:04-58:29, 58:31N-58:35, 58:37-58:43, 58:45-58:46, C*02:06w, C*02:47w
686 / 95 bp / 1070 bp / 8, 18, 22, 35, 37, 38, 39, 44, 51, 5102, 5103, 53, 78 / *07:65w, 07:134w, 08:32, 18:01:01:01-18:11, 18:13-18:15, 18:17N-18:36, 18:38-18:47, 18:49-18:65, 18:67-18:91, 35:01:01:01-35:08:04, 35:08:06-35:09:03, 35:11:01-35:12:03, 35:14:01-35:15, 35:17-35:18, 35:20:01-35:24:02, 35:27, 35:29:01-35:45, 35:48, 35:50-35:62, 35:64:01-35:68:02, 35:70-35:72, 35:74-35:75, 35:76w, 35:77-35:79, 35:81-35:153, 35:155-35:185, 35:187-35:190, 35:192-35:197, 35:199-35:206, 35:208-35:209, 35:211-35:216N, 35:218-35:225, 35:227-35:229, 35:231-35:233, 37:08, 38:06-38:07, 39:19:01-39:19:02, 44:06, 51:01:01-51:24:04, 51:26-51:46, 51:48-51:103,
51:105-51:111, 51:113-51:117, 51:119-51:146, 51:148-51:154, 53:01:01-53:16, 53:18-53:27, 53:29, 53:31, 56:06w, 78:01:01-78:04, 78:07
696,10 / 115 bp,
150 bp / 1070 bp / 15, 18,22, 27, 35, 39, 46, 55, 62, 72, 73, 75, 76 / *07:100, 13:31, 13:41, 15:01:01:01-15:01:04, 15:01:06-15:01:16, 15:01:18-15:01:30, 15:01:32-15:01:33, 15:04, 15:07:01-15:08, 15:11:01-15:12, 15:14-15:15, 15:19, 15:24:01-15:24:02, 15:26N-15:28, 15:30, 15:32, 15:34-15:35, 15:38:01-15:38:02, 15:43, 15:45-15:46, 15:50, 15:53-15:54, 15:56-15:58, 15:60, 15:63, 15:66, 15:68, 15:70-15:71, 15:73, 15:75-15:77, 15:79N, 15:81-15:82, 15:85, 15:87, 15:92, 15:94N, 15:96-15:97, 15:101-15:102, 15:104-15:105, 15:109-15:111N, 15:113, 15:117-15:118, 15:120, 15:122, 15:125-15:126, 15:128-15:129, 15:135, 15:137, 15:140, 15:142-15:149N, 15:152, 15:154, 15:157, 15:159-15:160, 15:163-15:167, 15:169, 15:171-15:172, 15:174-15:175, 15:178, 15:180-15:184, 15:187, 15:189-15:193, 15:201-15:203, 15:205-15:207, 15:209N, 15:211-15:212, 15:215, 15:217, 15:225, 15:227-15:228, 15:231-15:234, 15:236, 15:239, 15:241, 15:244-15:247, 15:251, 15:256-15:262N, 15:264, 15:267, 15:269-15:270, 15:272N, 15:276-15:280, 18:19, 27:25, 27:75, 35:14:01-35:14:02, 35:43:01-35:44, 35:62, 35:67, 35:79, 35:86, 35:102, 35:117, 35:135, 35:163, 35:185, 35:213, 39:18, 39:36, 44:146, 46:01:01-46:02, 46:04-46:05, 46:07N, 46:09-46:10, 46:12, 46:14-46:17, 46:20, 46:22-46:24, 46:26-46:32, 46:34-46:40, 46:42, 51:61:01-51:61:02, 52:21, 54:06, 55:21, 56:03, 73:01-73:02, A*26:68, A*68:56, C*02:56, C*06:20, C*12:50
7015 / 360 bp / 1070 bp / Bw4
71 / 350 bp / 1070 bp / Bw6
72 / 285 bp / 800 bp / 7, 703, 8, 35, 40, 41, 42, 48, 53, 60, 61, 81 / *07:02:01-07:02:38, 07:03-07:06, 07:08-07:18:02, 07:20-07:32, 07:34-07:39, 07:41-07:52, 07:54-07:59, 07:61-07:76, 07:79-07:83, 07:85-07:99, 07:101-07:121, 07:123-07:138, 07:140-07:164, 07:166-07:192, 07:194-07:195, 08:01:01-08:01:20, 08:02-08:05, 08:07-08:08N, 08:10-08:11, 08:13-08:15, 08:17-08:83, 08:85-08:88, 08:90-08:102, 08:104-08:106, 08:108-08:111, 35:66, 35:87, 37:07, 40:15-40:16, 40:30-40:32, 40:34, 40:45, 40:59, 40:80, 40:98, 40:137, 40:160:01-40:160:02, 41:02:01-41:02:05, 41:04, 41:10-41:11, 41:13, 41:18-41:19, 41:23-41:24, 41:27, 42:01:01-42:02, 42:05:01-42:07, 42:09-42:13, 42:15-42:20, 44:150, 44:166, 48:01:01-48:01:04, 48:05-48:12, 48:14-48:20, 48:22, 48:27-48:31, 53:15, 81:01-81:06

1Alleles are assigned by the presence of specific PCR product(s). However, the sizes of the specific PCR products may be helpful in the interpretation of HLA-B low resolution SSP typings.

Nonspecific amplifications, i.e. a ladder or a smear of bands, may sometimes be seen. GC-rich primers have a higher tendency of giving rise to nonspecific amplifications than other primers.

PCR fragments longer than the control bands may sometimes be observed. Such bands should be disregarded and do not influence the interpretation of the SSP typings.

Well number 25 contains the primer pair giving rise to the shorter, 800 bp, internal positive control band. In addition, wells number 28 to 30, 34, 41, 44, 45, 52, 54, 55, 59, 66 and 72 contain the primer pair giving rise to the shorter, 800 bp, internal positive control band.

In the presence of a specific amplification the intensity of the control band often decreases.

3The serological reactivity of all HLA-B alleles is not known. In this table we use the expert-assigned serological grouping in Tissue Antigens (2009)73:95-170 and the serological grouping of the sequence-defined allele.

5Due to the sharing of sequence motifs between HLA-Class I alleles, the C*02:23 and C*04:77 alleles will be amplified by primer mix 25, the C*03:05, C*03:25, C*03:27, C*03:143 and C*03:167 alleles will be amplified by primer mix 27, the C*01:30 and C*12:87 alleles will be amplified by primer mix 28, the A*23:31, A*24:106, C*07:231 and C*16:10 alleles will be amplified by primer mix 30, the C*01:73 allele will be amplified by primer mixes 31, 50 and 55, the C*07:02:30 and C*08:16:02 alleles will be amplified by primer mix 31, the C*07:46 allele will be amplified by primer mix 32, the A*24:174 allele will be amplified by primer mix 37, the C*03:102 allele will be amplified by primer mixes 41 and 65, the C*03:179 allele will be amplified by primer mix 43, the C*07:302 allele will be amplified by primer mix 44, the C*15:51 allele will amplified by primer mix 45, the C*03:129 and C*17:16 alleles will be amplified by primer mix 49, the C*15:25 allele will be amplified by primer mix 53, the C*15:39 allele will be amplified by primer mix 55, the C*15:02:04 allele will amplified by primer mix 64, the C*03:19 allele will be amplified by primer mix 65, the C*06:72 allele will be amplified by primer mix 66, the C*02:06 and 02:47 alleles will weakly be weakly amplified by primer mix 67 and the A*26:68, A*68:56, C*02:56, C*06:20 and C*12:50 alleles will be amplified by primer mix 69.

6HLA-specific PCR products shorter than 125 base pairs have a lower intensity and are less sharp than longer PCR products.

7Primer mix 28 may give rise to a lower yield of HLA-specific PCR product than the other HLA-B low resolution primer mixes in B*40, B*41, B*45, B*49 and B*50 alleles.

8Primer mixes 28, 29, 33, 53 and 63 may give rise to a lower yield of HLA-specific PCR product than the other HLA-B low resolution primer mixes.

9Primer mix 33 has pronounced tendencies of unspecific amplifications.

10Primer mixes 25, 43, 44, 50, 53 and 69 have a tendency to giving rise to primer oligomer formation, most pronounced in primer mix 53

11The B*57 and B*58 alleles might be faintly amplified by primer mix 33.

12The C*17:01 to C*17:04 alleles might be faintly amplified by primer mix 49.

13Primer mixes 56 and 67 may generate a false positive band of about 800 base pairs. This band should be disregarded when interpreting HLA-B low resolution typings.

14Primer mix 63 may give rise to a lower yield of B*54 alleles than the other B low primer mixes.

15The Bw4-associated HLA-A specificities A9, A23, A24, A2403, A25 and A32 are not amplified by the primer pair in primer mix 70.

16Primer mix 28 amplifies the B*15:143, 35:19, 40:170, 44:07 and 50:07 alleles in addition to the alleles specified in the table.

17Primer mix 50 amplifies the B*37:06:01 allele in addition to the alleles specified in the table.

‘w’, might be weakly amplified.

‘?’, nucleotide sequence information not available for the primer matching sequence.

Olerup SSP ABFor In Vitro Diagnostic Use

Franzengatan 5

SE-11251 Stockholm

Sweden

January 2015HLA-A-B-DR Combi TrayPage 1 of 1

Rev. No: 0391SWorksheet can be downloaded from

Worksheet

Olerup SSP ABFor In Vitro Diagnostic Use

Franzengatan 5

SE-11251 Stockholm

Sweden

January 2015HLA-A-B-DR Combi TrayPage 1 of 1

Rev. No: 0391SWorksheet can be downloaded from

Worksheet

Olerup SSP ABFor In Vitro Diagnostic Use

Franzengatan 5

SE-11251 Stockholm

Sweden

January 2015HLA-A-B-DR Combi TrayPage 1 of 1

Rev. No: 0391SWorksheet can be downloaded from

Worksheet

1The sequence of the B*0701 allele has been shown to be in error.

The sequence of the B*08:06 allele has been shown to be identical to B*08:20.

The sequence of the B*1305 allele has been shown to be identical to B*13:04.

The B*13:08Q allele has been renamed B*13:08.

The B*1324 allele has been renamed B*13:22:02

The B*150105 allele has been corrected and renamed B*15:120

The B*1522 allele has been renamed B*35:43.

The sequence of the B*1541 allele has been shown to be identical to B*15:39.

The B*1559 allele has been renamed B*35:44.

The B*15:100 allele has never been assigned.

The sequence of the B*1816 allele has been shown to be identical to B*18:14.

The sequence of the B*27051 allele has been shown to be identical to B*27:05:02.

The sequence of the B*2722 allele has been shown to be identical to the corrected B*27:06 sequence.

The B*3573 allele has been renamed B*35:08:03.

The B*35:43:02 allele has been renamed B*35:185.

The sequence of the B*39012 allele has been shown to be identical to B*39:01:01:01.

The sequence of the B*3921 allele has been shown to be identical to B*39:24.

The sequence of the B*4017 allele has been shown to be identical to B*40:16.

The sequence of the B*4041 allele has been shown to be identical to B*40:40.

The sequence of the B*4203 allele has never been assigned.

The sequence of the B*4401 allele has been shown to be identical to B*44:02:01:01.

The B*44:142Q allele has been renamed B*44:142.

The sequence of the B*5003 allele has been shown to be identical to B*50:02.

The sequence of the B*5125 allele has been shown to be identical to B*51:22.

The B*5147 allele has been renamed B*51:09:02.

The sequence of the B*5506 allele has been shown to be identical to B*55:04.

The sequence of the B*5803 allele has never been assigned.

The sequence of the B*58:30 allele has been shown to be identical to B*58:01:01.

The B*7901 allele has been renamed B*15:18:01.

The B*9530 allele has been renamed B*15:27:02.

2The serological reactivity of all HLA-B alleles is not known. In this table we use the expert-assigned serological grouping in Tissue Antigens (2009)73:95-170 and the serological grouping of the sequence-defined allele.

3The B*08:26, 08:50, 08:62, 08:85, 08:94 and 42:07 alleles give rise to identical amplification patterns with the HLA-B low resolution primer set. These alleles can be separated by the respective high resolution primer sets.

4The B*13:21, 13:35 and 13:59 and the B*44:135 and 44:158 alleles give rise to identical amplification patterns with the HLA-B low resolution primer set. These alleles can be separated by the respective high resolution primer sets.

5The B*14:08 and the B*39:25N, 39:30, 39:32-39:34, 39:47, 39:50, 39:74 and 39:82 alleles give rise to identical amplification patterns with the HLA-B low resolution primer set. These alleles can be separated by the respective high resolution primer sets.

6The B*18:29 and 18:72 and the B*35:32:01-35:32:02, 35:37, 35:53N, 35:64:01-35:64:02, 35:68:01-35:68:02, 35:99, 35:118-35:119 and 35:174 alleles give rise to identical amplification patterns with the HLA-B low resolution primer set. These alleles can be separated by the respective high resolution primer sets.

7The B*41:09 and the B*45:02-45:03 alleles give rise to identical amplification patterns with the HLA-B low resolution primer set. These alleles can be separated by the respective high resolution primer sets.

8The B*54:01:02 and the B*55:01:07, 55:02:01-55:02:06, 55:07, 55:10, 55:12, 55:16, 55:19, 55:26, 55:30, 55:35, 55:37, 55:39, 55:41-55:43, 55:47-55:48, 55:50 and 55:57 alleles give rise to identical amplification patterns with the HLA-B low resolution primer set. These alleles can be separated by the respective high resolution primer sets.

9The B*55:04, 55:08, 55:13, 55:27, 55:46 and 55:49 and the B*56:01:05, 56:15, 56:19N, 56:22 alleles give rise to identical amplification patterns with the HLA-B low resolution primer set. These alleles can be separated by the respective high resolution primer sets.

10The B*55:23 and 55:32 and the B*56:18 and 56:31-56:32 alleles give rise to identical amplification patterns with the HLA-B low resolution primer set. These alleles can be separated by the respective high resolution primer sets.

11Primer mix 28 amplifies the B*15:143, 35:19, 40:170, 44:07 and 50:07 alleles, and primer mix 50 amplifies the B*37:06:01 allele in addition to the alleles specified in the tables.

‘ser’, serological HLA specificity.

‘w’, might be weakly amplified.

‘?’, nucleotide sequence information not available for the primer matching sequence.

Olerup SSP ABFor In Vitro Diagnostic Use

Franzengatan 5

SE-11251 Stockholm

Sweden

January 2015HLA-A-B-DR Combi TrayPage 1 of 1

Rev. No: 0391SWorksheet can be downloaded from

Worksheet

Specificities and sizes of the PCR products of the 23+1 primer mixes used for DR low resolution SSP typing.

Primer Mix / Size of spec. PCR product1 / Size of control band2 / DR
serology3 / Amplified HLA-DRB alleles4,5
737,8 / 205 bp,
255 bp / 515 bp / 1 / *01:01:01-01:02:08, 01:04-01:38, 01:40N-01:59
74 / 200 bp / 430 bp / 103 / *01:03, 01:39N, 01:42
757 / 200 bp,
220 bp / 430 bp / 15 / *15:01:01:01-15:98
76 / 210 bp / 430 bp / 16 / *16:01:01-16:05:02, 16:07-16:24
775,6,7 / 120 bp,
220 bp / 430 bp / 3, 17, 18, 11 / *03:01:01:01-03:75, 03:77-03:84, 03:86-03:93, 11:07, 11:53, 11:103, 11:105, 11:107, 11:125, 15:25
785,6,7 / 80 bp,
210 bp / 430 bp / 3, 17, 11, 13, 14, 6 / *03:01:01:01-03:01:22, 03:04:01-03:06, 03:08-03:16, 03:18-03:20, 03:22-03:23, 03:25-03:26, 03:28, 03:30-03:31, 03:33-03:34, 03:36-03:37, 03:43-03:48, 03:50-03:52, 03:54-03:68N, 03:70-03:73, 03:75-03:84, 03:86, 03:89, 03:91-03:93, 08:40, 11:02:01-11:03, 11:11:01-11:11:02, 11:14:01-11:14:02, 11:16, 11:20-11:21, 11:36, 11:40-11:41, 11:48, 11:59, 11:63, 11:65:01-11:65:02, 11:68, 11:70, 11:73, 11:76, 11:79-11:80, 11:83, 11:85-11:87, 11:93, 11:118, 11:122, 11:124, 11:127, 11:131-11:132, 11:135, 11:138-11:139, 11:142, 13:01:01-13:04, 13:08, 13:10, 13:15-13:17, 13:19-13:20, 13:22-13:24, 13:27-13:29, 13:31-13:41, 13:43, 13:45, 13:48, 13:51-13:54, 13:57, 13:59, 13:61:01-13:61:02, 13:63-13:66:02, 13:68-13:76, 13:78-13:81, 13:83-13:85, 13:87-13:99, 13:101-13:102, 13:104-13:107, 13:109, 13:111-13:117, 13:120-13:131, 13:133, 13:135, 13:137N-13:145, 13:147-13:149, 13:151-13:153, 13:155, 13:159, 13:162, 14:16, 14:19, 14:21, 14:82, 14:95, 14:109, 14:132, 14:137N
796,7 / 85 bp,
210 bp / 430 bp / 3, 18, 11, 13, 14, 6 / *03:02:01-03:03, 03:27, 03:29, 03:38, 03:53, 03:74, 03:88, 03:90, 11:13:01w-11:13:02w, 11:26, 11:34, 13:15, 13:19, 13:26:01-13:26:02, 13:44, 13:53, 13:57, 13:85-13:86, 13:104, 14:02-14:03:02, 14:06:01-14:06:02, 14:09, 14:12:01-14:13, 14:17-14:21, 14:24, 14:27, 14:29-14:30, 14:32:01w-14:32:02w, 14:33, 14:40-14:41, 14:47-14:49, 14:51, 14:63, 14:65w, 14:67, 14:77-14:78, 14:80-14:81, 14:83, 14:85, 14:89, 14:94, 14:98, 14:102, 14:106, 14:108-14:109, 14:115, 14:119, 14:121, 14:135
806,7,8 / 100 bp,
175 bp / 430 bp / 3,4 / *04:01:01-04:05:11, 04:05:13-04:163
817 / 210 bp,
235 bp / 430 bp / 7, 13, 14 / *07:01:01:01-07:01:07, 07:03-07:26N, 12:22, 13:17, 13:116, 14:50
827 / 170 bp,
215 bp,
250 bp / 515 bp / 8, 12, 14 / *08:01:01-08:19, 08:21-08:54, 11:67, 12:04, 12:16:01-12:16:02, 12:22, 12:39, 14:11, 14:15, 14:68, 14:93
836,7 / 90 bp,
135 bp,
180 bp / 430 bp / 3, 9, 11 / *03:08, 03:65, 09:01:02-09:20, 11:07, 11:53, 11:103, 11:105, 11:107, 11:125
84 / 175 bp / 430 bp / 10,11,13 / *03:76, 10:01:01-10:06, 11:59, 11:80, 11:83, 11:87, 11:135, 11:142, 13:27, 13:41, 13:71, 13:129
856,7 / 100 bp,
170 bp / 430 bp / 11, 3, 8 / *03:08, 03:65, 08:31, 08:41, 11:01:01-11:70, 11:72-11:147
866,7 / 85 bp,
105 bp / 430 bp / 12, 8 / *08:32, 08:53, 12:01:01-12:40
878 / 215 bp / 430 bp / 6, 8, 11, 13, 14, 1403 / *03:76, 08:20-08:21, 11:01:01-11:04:11, 11:06:01-11:06:03, 11:08:01-11:12:02, 11:14:01-11:16, 11:18-11:21, 11:23:01-11:25, 11:27:01-11:33, 11:35-11:51, 11:54:01-11:54:02, 11:56-11:66, 11:68, 11:70, 11:72-11:81, 11:83-11:88, 11:90-11:97, 11:99-11:102:02, 11:106, 11:108-11:124, 11:126-11:135, 11:137-11:142, 11:144-11:147, 13:01:01-13:02:01, 13:02:03-13:08, 13:10-13:16, 13:18-13:43, 13:45-13:85, 13:87-13:115, 13:117-13:128, 13:130-13:145, 13:147-13:164, 14:03:01-14:03:02, 14:12:01-14:12:02, 14:16, 14:19, 14:21-14:22, 14:25, 14:27, 14:40, 14:53, 14:63, 14:67, 14:69, 14:74, 14:77-14:78, 14:84-14:85, 14:98, 14:102, 14:105, 14:109, 14:115-14:116, 14:128, 14:135, 14:137N, DRB3*02:27
887,8 / 195 bp,
225 bp / 430 bp / 13, 8, 11, 12, 14, 6 / *08:01:01-08:02:04, 08:04:01-08:09, 08:11, 08:16-08:17, 08:20-08:22, 08:24, 08:26, 08:28, 08:31, 08:39, 08:41-08:44, 08:50, 08:52, 08:54, 11:01:01-11:01:17, 11:02:01-11:06:03, 11:09-11:12:02, 11:14:01-11:16, 11:20-11:21, 11:23:01-11:25, 11:27:01-11:30, 11:32-11:33, 11:35-11:41, 11:43-11:44, 11:46:01-11:51, 11:54:01-11:56, 11:58:01-11:63, 11:65:01-11:70, 11:72, 11:74:01-11:78, 11:80-11:88, 11:90-11:97, 11:99-11:102:02, 11:106, 11:108-11:118, 11:120-11:124, 11:126-11:129, 11:133-11:135, 11:137-11:142, 11:144-11:147, 12:02:01-12:02:05, 12:13, 12:15-12:16:02, 12:18-12:21, 12:23, 12:26-12:27, 12:31N-12:33, 12:37, 13:01:01-13:02:01, 13:02:03-13:02:08, 13:04-13:05:02, 13:07:01-13:09, 13:11:01-13:11:02, 13:14:01-13:24, 13:26:01-13:29, 13:31-13:32, 13:34-13:36, 13:38-13:43, 13:45-13:55, 13:57, 13:59, 13:61:01-13:65, 13:67-13:76, 13:78-13:80, 13:83-13:84, 13:87, 13:91-13:93, 13:96:01-13:100, 13:102-13:109, 13:111-13:114, 13:116-13:117, 13:121, 13:123-13:132, 13:135-13:136, 13:138-13:150, 13:153, 13:155, 13:158-13:160, 13:162, 13:164, 14:15-14:16, 14:22, 14:24-14:25, 14:27, 14:37, 14:53, 14:73, 14:105, 14:128
895 / 175 bp,
240 bp / 430 bp / 3, 13, 14, 17, 6, 11 / *03:01:01:01-03:01:05, 03:01:07-03:01:08, 03:01:10-03:07, 03:09, 03:11:01-03:41, 03:43-03:45, 03:47-03:63, 03:66-03:68N, 03:70-03:84, 03:86, 03:88-03:91, 03:93, 08:20, 11:13:01-11:13:02, 13:01:01-13:16, 13:18-13:42, 13:44, 13:46-13:66:02, 13:68-13:102, 13:104-13:115, 13:117-13:121, 13:123-13:158, 13:161-13:164, 14:01:01-14:07:02, 14:08?, 14:09-14:14, 14:15?-14:16?, 14:17-14:21, 14:22?, 14:23:01, 14:23:02?, 14:23:03-14:24, 14:25?-14:26?, 14:27, 14:28?, 14:29-14:30, 14:31?-14:32:03?, 14:33, 14:34?-14:35?, 14:36-14:37, 14:38?-14:39?, 14:40-14:45, 14:47-14:48, 14:49?-14:50?, 14:51, 14:52?-14:53?, 14:54:01-14:54:02, 14:55?, 14:56-14:57, 14:58?, 14:59, 14:60?-14:62?, 14:63-14:65, 14:67, 14:68?-14:76?, 14:77-14:78, 14:79?, 14:80-14:85, 14:86?-14:88?, 14:89, 14:90?, 14:91, 14:92N?-14:93?, 14:94-14:96, 14:97?, 14:98, 14:99?, 14:100, 14:101?, 14:102-14:103, 14:104?-14:105?, 14:106, 14:107?, 14:108-14:109, 14:110?-14:114?, 14:115-14:116, 14:117?-14:120?, 14:121, 14:122?, 14:123, 14:124?-14:126?, 14:127:01-14:127:02, 14:128?-14:133?, 14:134-14:137N, 14:138?-14:140?, 14:141, 14:142?
906,7 / 100 bp,
140 bp,
155 bp / 430 bp / 14, 4, 8,13, 6 / *04:62, 04:69, 04:73, 04:105:01-04:105:02, 04:122, 04:146, 08:08, 11:69, 11:82, 13:45, 14:01:01-14:01:02, 14:01:04, 14:04, 14:07:01-14:07:02, 14:10, 14:16, 14:22, 14:25-14:26, 14:28, 14:31-14:32:03, 14:35, 14:37-14:39, 14:49-14:50, 14:53-14:54:01, 14:55,
14:57-14:58, 14:60-14:62, 14:68-14:71, 14:73-14:76, 14:79, 14:82, 14:86-14:88, 14:90, 14:93, 14:99, 14:101, 14:104-14:105, 14:107, 14:110-14:114, 14:117-14:120, 14:122, 14:124-14:125, 14:128-14:129, 14:131, 14:137N-14:140, 14:142, DRB4*01:03:01:02N
916,7,9 / 110 bp,
135 bp,
170 bp / 430 bp / 14, 3, 9, 11, 12, 13, 15, 6, 4 / *03:10, 09:01:02-09:01:05, 09:01:07-09:02:02, 09:04-09:20, 11:13:01-11:13:02, 11:17, 11:52, 13:43, 13:159, 14:01:01-14:02, 14:04-14:11, 14:13-14:14, 14:16-14:18, 14:19w, 14:20, 14:21w, 14:22-14:23:04, 14:26, 14:28-14:36, 14:38-14:39, 14:41, 14:43-14:52, 14:54:01-14:57, 14:59-14:62, 14:64-14:65, 14:68, 14:70-14:76, 14:79-14:83, 14:86-14:88, 14:90-14:97, 14:99-14:101, 14:103-14:108, 14:109w, 14:110-14:114, 14:117-14:127:02, 14:129-14:134, 14:137N-14:140, 14:142, 15:27, 15:34, 15:66
926,7,8 / 110 bp,
175 bp,
225 bp / 430 bp / 14, 3, 8, 11, 13, 15, 16, 6, 2 / *03:10, 08:09, 08:20-08:21, 08:32, 08:35, 08:36:02, 08:53, 11:13:01-11:13:02, 11:17, 11:23:01-11:23:02, 11:25, 11:31, 11:45, 11:52, 11:55, 11:64, 11:89, 11:96, 11:119, 13:13, 13:18, 13:43, 13:45, 13:47, 13:55, 13:119, 13:144, 13:146, 13:154, 13:156, 13:158-13:159, 13:164, 14:01:01-14:01:04, 14:03:01-14:05:04, 14:07:01-14:08, 14:10-14:12:02, 14:14-14:16, 14:18, 14:22-14:23:04, 14:25-14:28, 14:31-14:32:03, 14:34-14:36, 14:38-14:40, 14:42-14:45, 14:49-14:50, 14:53-14:65, 14:67-14:79, 14:81-14:82, 14:84-14:93, 14:95-14:97, 14:99-14:105, 14:107, 14:110-14:120, 14:122-14:140, 14:142, 15:21w, 16:04w, 16:18w
937,8 / 160 bp,
240 bp / 430 bp / 52 / *14:141, DRB3*01:01:02:01-01:15, DRB3*02:01-02:29N, DRB3*03:01:01-03:03
948,10 / 215 bp / 430 bp / 53 / DRB4*01:01:01:01-01:08
95 / 175 bp / 430 bp / 51 / DRB5*01:01:01-01:14, DRB5*02:02-02:06
9611 / Negative control

1Alleles are assigned by the presence of specific PCR product(s). However, the sizes of the specific PCR products may be helpful in the interpretation of DR low resolution SSP subtypings.

Nonspecific amplifications, i.e. a ladder or a smear of bands, may sometimes be seen. GC-rich primers have a higher tendency of giving rise to nonspecific amplifications than other primers, e.g. the primers in wells 75 and 90 to 92.

PCR fragments longer than the control bands may sometimes be observed. Such bands should be disregarded and do not influence the interpretation of the SSP typings.