Response of Bacterial Community Compositions to Different Sources of Pollutants in Sediments of a Tributary of Taihu Lake, China

Jing Wang, Yi Li*, Peifang Wang, Lihua Niu*, Wenlong Zhang, Chao Wang

Key Laboratory of Integrated Regulation and Resource Development on Shallow Lakes, Ministry of Education, College of Environment, Hohai University, Xikang Road #1, Nanjing 210098, P.R. China

* Corresponding author:

Dr. Yi Li

Tel: +86 013776417768

Fax: 86-25-83786251

E-mail: .

Address: Ministry of Education Key laboratory of integrated regulation and resource development on shallow lakes, College of Environment, Hohai University, Nanjing 210098, P.R. China

Dr. Lihua Niu

E-mail address: .

Address: Ministry of Education Key laboratory of integrated regulation and resource development on shallow lakes, College of Environment, Hohai University, Nanjing 210098, P.R. China

Table and Figure Captions

Table S1 The top 10 abundant tRFs in each mixed sample revealed by the T-RFLP profiles with Hae III enzymes. The phylogenetic taxa at the phylum level were determined based on the combination of T-RFLP and clone library.

Fig. S1 Relative abundances of bacterialphyla and subphyla reflected by the 16S rRNA clone library.

Fig. S2 Clustering analysis of the ten mixed samples at the T-RFLP profiles level based on the Bray-Curtis similarity.

Fig. S3 T-RFLP profiles of ten mixed samples digested by Hae III enzymes.

Table S1 The top 10 abundant tRFs of each sample’s sediment bacterial communities revealed by T-RFLP profiles with Hae III enzymes. The relative abundance of bacteria communities in each sampling site were calculated by their average percentages in the total sequence of the triplicate samples. The phylogenetic taxa at the phylum level were determined based on the combination of T-RFLP and clone library.

T-RF(bp) / Bacterial
Phylum / Bacterial
Class / Bacterial
genus / U1 / U2 / W3 / W4 / W5 / W6 / S7 / S8 / A9 / A10
50 / Firmicutes / Bacilli / Paenibacillus
Geobacillus / 1.89 / 0.00 / 3.67 / 2.54 / 1.72 / 0.99 / 0.00 / 0.00 / 2.70 / 2.46
64 / Firmicutes / Clostridia / Caldanaerobacter
Carboxydibrachium
Thermoanaerobacter / 2.50 / 2.38 / 0.00 / 1.38 / 1.63 / 2.19 / 3.25 / 0.00 / 3.05 / 3.62
68 / Bacteroidetes / Flavobacteria / Flavobacterium
Chryseobacterium / 0.00 / 0.00 / 0.00 / 1.19 / 0.00 / 0.00 / 2.80 / 0.00 / 4.98 / 4.56
69 / Proteobacteria / Alphaproteobacteria / Azospirillum
Bifidobacterium
Methylobacteriaceae / 3.89 / 3.70 / 6.25 / 5.39 / 5.46 / 5.09 / 2.50 / 3.36 / 4.63 / 3.88
70 / Bacteroidetes / Sphingobacteria / Dyadobacter
Leadbetterella / 3.45 / 3.27 / 5.46 / 3.77 / 4.79 / 4.48 / 0.00 / 3.01 / 0.00 / 0.00
71 / Proteobacteria / Alphaproteobacteria / Azospirillum
Blastomonas
Sphingomonas / 3.22 / 3.22 / 3.32 / 3.74 / 3.76 / 3.86 / 3.42 / 3.59 / 3.79 / 3.29
192 / Firmicutes / Clostridia / Eubacterium
Selenomonas / 2.55 / 2.46 / 0.00 / 1.10 / 1.69 / 2.27 / 2.60 / 3.37 / 3.13 / 2.88
194 / Actinobacteria / Actinobacteria / Actinomyces
Patulibacter
Prauserella / 3.47 / 3.50 / 4.41 / 4.55 / 4.46 / 4.41 / 3.53 / 3.81 / 3.98 / 3.61
198 / Proteobacteria / Betaproteobacteria / Acidovorax
Burkholderiaceae
Comamonadaceae
Janthinobacterium / 4.06 / 3.82 / 3.75 / 4.34 / 4.37 / 4.52 / 4.11 / 4.07 / 4.37 / 3.89
199 / Proteobacteria / Betaproteobacteria / Bordetella
Burkholderiales
Chromobacterium
Cupriavidus / 3.26 / 3.31 / 5.16 / 4.89 / 4.68 / 4.43 / 3.10 / 3.25 / 3.80 / 4.27
206 / Proteobacteria / Alphaproteobacteria / Erythrobacter
Nitratireductor
Rhodobaca / 3.31 / 3.57 / 3.95 / 4.27 / 4.29 / 4.35 / 3.63 / 3.25 / 4.07 / 3.97
210 / Actinobacteria / Actinobacteria / Rubrobacter
Frankia / 2.89 / 2.93 / 4.20 / 4.25 / 3.97 / 3.83 / 3.16 / 3.17 / 3.42 / 2.91
216 / Actinobacteria / Actinobacteria / Collinsella
Leucobacter
Mobiluncus
Mycobacterium / 0.00 / 2.58 / 0.00 / 0.00 / 1.77 / 2.38 / 0.00 / 2.20 / 0.92 / 3.78
219 / Proteobacteria / Betaproteobacteria / Achromobacter
Burkholderia
Janthinobacterium
Ralstonia / 3.70 / 3.79 / 5.77 / 5.69 / 5.29 / 5.04 / 4.02 / 3.59 / 3.97 / 4.49
220 / Proteobacteria / Betaproteobacteria / Variovorax
Ramlibacter / 3.21 / 0.00 / 0.00 / 1.42 / 0.00 / 0.00 / 3.33 / 3.47 / 3.61 / 0.00
221 / Nitrospira / Nitrospira / Leptospirillum
Thermodesulfovibrio / 0.00 / 3.41 / 4.07 / 2.81 / 4.24 / 4.23 / 0.00 / 0.00 / 0.00 / 0.00
233 / Actinobacteria / Actinobacteria / Arcanobacterium
Mycobacterium / 3.10 / 3.16 / 4.83 / 4.62 / 4.42 / 4.21 / 3.02 / 3.20 / 3.67 / 3.26
240 / Firmicutes / Clostridia / Halobacteroides
Clostridium
Thermoanaerobacterium / 2.35 / 2.40 / 0.00 / 1.19 / 1.65 / 2.21 / 2.81 / 3.33 / 2.85 / 0.00
241 / Actidobacteria / Acidobacteria / Acidobacterium / 0.00 / 1.48 / 0.00 / 1.03 / 1.02 / 1.37 / 2.41 / 2.88 / 2.55 / 2.75
253 / Proteobacteria / Deltaproteobacteria / Geobacter / 3.40 / 3.27 / 0.00 / 1.46 / 2.24 / 3.01 / 3.43 / 3.36 / 3.58 / 2.97
260 / Firmicutes / Bacilli / Alicyclobacillus
Staphylococcus / 2.98 / 3.26 / 0.00 / 1.38 / 2.23 / 3.00 / 3.25 / 2.75 / 3.31 / 2.86
307 / Bacteroidetes / Flavobacteria / Psychromonas
Flavobacterium / 0.00 / 0.00 / 4.66 / 3.22 / 2.18 / 1.25 / 0.00 / 0.00 / 0.00 / 0.00
308 / Actinobacteria / Actinobacteria / Actinosynnema
Frankia
Rubrobacter / 3.29 / 3.15 / 5.90 / 5.09 / 4.91 / 4.49 / 2.38 / 3.28 / 4.36 / 3.19
309 / Firmicutes / Bacilli / Staphylococcus
Paenibacillus / 0.00 / 0.00 / 0.00 / 0.00 / 0.00 / 0.00 / 0.00 / 0.00 / 2.88 / 0.00
310 / Bacteroidetes / Flavobacteria / Empedobacter
Flavobacterium / 2.41 / 0.00 / 4.97 / 3.44 / 2.32 / 1.34 / 0.00 / 0.00 / 2.87 / 0.00
314 / Firmicutes / Clostridia / Thermoanaerobacter
Desulfotomaculum / 2.51 / 2.39 / 4.75 / 3.28 / 3.86 / 3.48 / 0.00 / 0.00 / 2.68 / 0.00
316 / Firmicutes / Clostridia / Clostridiales
Desulfotomaculum
Ruminococcus / 0.00 / 2.74 / 4.35 / 3.01 / 3.91 / 3.70 / 0.00 / 2.75 / 3.46 / 0.00
331 / Nitrospira / Nitrospira / Gallionella
Leptospirillum / 2.37 / 2.49 / 4.59 / 3.17 / 3.85 / 3.53 / 0.00 / 2.29 / 3.54 / 0.00
402 / Proteobacteria / Alphaproteobacteria / Ehrlichia
Magnetospirillum / 3.58 / 3.73 / 4.95 / 5.08 / 4.87 / 4.77 / 3.89 / 3.43 / 3.84 / 4.42
409 / Firmicutes / Clostridia / Clostridium
Desulfotomaculum / 2.64 / 0.00 / 0.00 / 0.98 / 0.00 / 0.00 / 2.30 / 2.55 / 0.00 / 0.00

Fig. S1Relative abundances of bacterial phyla and subphyla reflected by the 16S rRNA clone library.

Fig. S2 Clustering analysis of the ten samples at the T-RFLP profiles level based on the Bray-Curtis similarity.



Fig. S3 T-RFLP profiles of ten mixed samples digested by Hae III enzymes.