Supplementary Table 1. Functional domain analysis and comparison to protein domains in eight other fungi.

Ashgo, Ashbya gossypii; Canal, Candida albicans; Cangl = Candid glabrata; Debha, Debaryomyces hansenii;Klua, Klyveromyces lactis; Picst, Pichia stipitis; Sacce, Saccharomyces cerevisiae; Schpo, Schizosaccharomyces pombe; Yarli, Yarrowia lipolytica

Total number of distinct pfam domains for different fungi

AshgoCanalCanglDebhaKlulaPicstSacceSchpoYarli

169316731696175417431712182318201749

Number of pfam domains shared by P. stipitis with other fungi

AshgoCanalCanglDebhaKlulaPicstSacceSchpoYarli

155515961551163915901712160215341589

4 pfam domains present in P. stipitis, but absent in other 8 fungi

AshgoCanalCanglDebhaKlulaPicstSacceSchpoYarliDomain

PF02129000001000Peptidase_S15

PF03702000001000UPF0075

PF00331000001000Glyco_hydro_10

PF08129000001000Antimicrobial17

21 Conserved fungal domains absent in P. stipitis

AshgoCanalCanglDebhaKlulaPicstSacceSchpoYarliDomain

PF03159222220222XRN_N

PF04098222220221Rad52_Rad22

PF00536111110143SAM_1

PF08226111110131DUF1720

PF01020111110221Ribosomal_L40e

PF03556111110111DUF298

PF05206111110111DUF715

PF03997111110111VPS28

PF02268111110111TFIIA_gamma_N

PF08228111110111RNase_P_pop3

PF05492111110111NAF1

PF02517111110111Abi

PF02970111110111TBCA

PF07572111110111BCNT

PF00475111110111IGPD

PF07957111110111Ribosomal_MRP8

PF02270111110111TFIIF_beta

PF07541111110111EIF_2_alpha

PF03801111110111Ndc80_HEC

PF05916111110111Sld5

List of top Pfam domains in P. stipitis:

DescriptionOccurrences

PF00069Protein kinase domain89

PF00400WD domain, G-beta repeat82

PF00172Fungal Zn(2)-Cys(6) binuclear cluster domain74

PF07690Major Facilitator Superfamily61

PF00271Helicase conserved C-terminal domain53

PF00083Sugar (and other) transporter45

PF04082Fungal specific transcription factor domain44

PF00076RNA recognition motif. (a.k.a. RRM, RBD, or RNP domain)41

PF00270DEAD/DEAH box helicase35

PF00096Zinc finger, C2H2 type34

PF00153Mitochondrial carrier protein34

PF00005ABC transporter30

PF02985HEAT repeat30

PF00004ATPase family associated with various cellular activities (AAA)28

PF00560Leucine Rich Repeat27

PF00324Amino acid permease25

PF00106short chain dehydrogenase24

PF00097Zinc finger, C3HC4 type (RING finger)24

PF00226DnaJ domain23

PF00018SH3 domain21

PF00071Ras family21

PF08240Alcohol dehydrogenase GroES-like domain21

PF00702haloacid dehalogenase-like hydrolase17

PF08242Methyltransferase domain16

PF01370NAD dependent epimerase/dehydratase family16

PF00107Zinc-binding dehydrogenase16

PF00248Aldo/keto reductase family16

PF00176SNF2 family N-terminal domain16

PF00561alpha/beta hydrolase fold16

PF00646F-box domain15

PF08241Methyltransferase domain15

PF07719Tetratricopeptide repeat15

PF00227Proteasome A-type and B-type14

PF00173Cytochrome b5-like Heme/Steroid binding domain14

PF00009Elongation factor Tu GTP binding domain14

PF07728ATPase family associated with various cellular activities (AAA)14

PF00149Calcineurin-like phosphoesterase14

PF07653Variant SH3 domain14

PF00300Phosphoglycerate mutase family13

PF00023Ankyrin repeat13

PF07714Protein tyrosine kinase13

PF00724NADH:flavin oxidoreductase / NADH oxidase family13

PF01926GTPase of unknown function13

PF00179Ubiquitin-conjugating enzyme12

PF00628PHD-finger12

PF01423LSM domain12

PF00125Core histone H2A/H2B/H3/H412

PF00515Tetratricopeptide repeat11

PF00249Myb-like DNA-binding domain11

PF00664ABC transporter transmembrane region11

PF00169PH domain11

PF00155Aminotransferase class I and II11

PF03144Elongation factor Tu domain 211

PF00583Acetyltransferase (GNAT) family11

74 Pfam Domains absent in D. hansenii but present in P. stipitis

AshgoCanalCanglDebhaKlulaPicstSacceSchpoYarliDomain

PF07691012036610PA14

PF07723210052121LRR_2

PF06814112011221Lung_7-TM_R

PF07529102001222HSA

PF01168011011141Ala_racemase_N

PF03835111011121Rad4

PF07544102011211CSE2

PF02811111011112PHP

PF04506111011111Rft-1

PF03986111011111Autophagy_N

PF03850111011111Tfb4

PF04840111011111Vps16_C

PF04190111011111DUF410

PF05176111011111ATP-synt_10

PF06423111011111GWT1

PF03870111011111RNA_pol_Rpb8

PF02469011011112Fasciclin

PF03839111011111Sec62

PF01974111021101tRNA_int_endo

PF02861111011111Clp_N

PF02185101011121HR1

PF03980111011111Nnf1

PF01213111011111CAP

PF07522101011111DRMBL

PF04090111011110RNA_pol_I_TF

PF02330111011110MAM33

PF05285101011111SDA1

PF05620011011111DUF788

PF03051111001102Peptidase_C1_2

PF00658111001111PABP

PF07885011011102Ion_trans_2

PF04841101011111Vps16_N

PF03982101011111DAGAT

PF07962111011101Swi3

PF05843101011111Suf

PF08286101011111Spc24

PF05832101011111DUF846

PF05022111011110SRP40_C

PF04428001011211Choline_kin_N

PF05967011011111DUF887

PF04182101011110B-block_TFIIIC

PF05141111011100DIT1_PvcA

PF06432110001111GPI2

PF01608011001111I_LWEQ

PF00994101011101MoCF_biosynth

PF05132101001111RNA_pol_Rpc4

PF05093100011111DUF689

PF01627011011110Hpt

PF04603001011111Mog1

PF06516020002010NUP

PF07923100011110N1221

PF04547100001111DUF590

PF00576010011011Transthyretin

PF01590010001021GAF

PF07904100011110CT20

PF01480010001011PWI

PF07061100001110DUF1337

PF05345110001100He_PIG

PF06427010001011UDP-g_GGTase

PF02875100011100Mur_ligase_C

PF07928000001011Vps54

PF02453011001000Reticulon

PF04106000001011APG5

PF06831010001001H2TH

PF03069010001010FmdA_AmdA

PF06032100011000DUF917

PF01661010001000A1pp

PF05699000011000hATC

PF06807000001010Clp1

PF02129000001000Peptidase_S15

PF03702000001000UPF0075

PF00331000001000Glyco_hydro_10

PF08129000001000Antimicrobial17

116 Pfam Domains present in D. hansenii but absent in P. stipitis

AshgoCanalCanglDebhaKlulaPicstSacceSchpoYarliDomain

PF00665051400441112rve

PF077270303104200RVT_2

PF03159222220222XRN_N

PF00230204120313MIP

PF04098222220221Rad52_Rad22

PF03595011210171C4dic_mal_tran

PF00536111110143SAM_1

PF00633022310131HHH

PF03731201210221Ku_N

PF05131051110111Pep3_Vps18

PF08226111110131DUF1720

PF04185100110007Phosphoesterase

PF01020111110221Ribosomal_L40e

PF03556111110111DUF298

PF05206111110111DUF715

PF03997111110111VPS28

PF01465111100121GRIP

PF05172102110210MPPN

PF02268111110111TFIIA_gamma_N

PF08228111110111RNase_P_pop3

PF04667011110211Endosulfine

PF05492111110111NAF1

PF02517111110111Abi

PF02970111110111TBCA

PF07572111110111BCNT

PF00475111110111IGPD

PF07957111110111Ribosomal_MRP8

PF02270111110111TFIIF_beta

PF04425102110300Bul1_N

PF00595111210200PDZ

PF07541111110111EIF_2_alpha

PF03801111110111Ndc80_HEC

PF05916111110111Sld5

PF00251100110121Glyco_hydro_32N

PF08058101110111NPCC

PF05365101110111UCR_UQCRX_QCR9

PF06331101110111REX1

PF00041110100112fn3

PF07558101110120Shugoshin_N

PF04032110110111Rpr2

PF07743101110111HSCB_C

PF03657111110101UPF0113

PF03126101110111Plus-3

PF04119011110111HSP9_HSP12

PF04869101110111Uso1_p115_head

PF07574111110110SMC_Nse1

PF04627021110110ATP-synt_Eps

PF04112011110111Mak10

PF00135000110014COesterase

PF05185101110111PRMT5

PF07683011120011CobW_C

PF06127011110110DUF962

PF04147101110110Nop14

PF03215101110110Rad17

PF0209610110011160KD_IMP

PF00444110110110Ribosomal_L36

PF03941101100111INCENP_ARK-bind

PF07106101110110TBPIP

PF03656101110110Pam16

PF04882101110101Peroxin-3

PF05238101110110CHL4

PF00077101110101RVP

PF01997010100022Translin

PF08296101110110SNM1

PF05712000110111MRG

PF02935100110110COX7C

PF02179001100111BAG

PF01027000110111UPF0005

PF08209101110100Sgf11

PF07971110110000Glyco_hydro_92

PF02320101110000UCR_hinge

PF03732100200001Retrotrans_gag

PF01244000110020Peptidase_M19

PF02617101100100ClpS

PF06957110100010COPI_C

PF00116100100110COX2

PF07189100100011SF3b10

PF04420010100110CHD5

PF07297010100011DPM2

PF03879100100110Cgr1

PF08244100100101Glyco_hydro_32C

PF03441000110100FAD_binding_7

PF04057010100010Rep-A_N

PF044190001100104F5

PF00165010100001HTH_AraC

PF08227010100010DUF1721

PF05368000300000NmrA

PF01977010100100UbiD

PF00875000110100DNA_photolyase

PF06172000110100Cupin_5

PF05388000100100Carbpep_Y_N

PF08193000100010DUF1711

PF06244010100000DUF1014

PF01476010100000LysM

PF02464000100001CinA

PF07393000100010Sec10

PF07519000200000Tannase

PF07665000100010MpPF2

PF02065000100010Melibiase

PF06645000100100SPC12

PF07632000100000DUF1593

PF07470000100000Glyco_hydro_88

PF00061000100000Lipocalin

PF01391000100000Collagen

PF08212000100000Lipocalin_2

PF01581000100000FARP

PF04892000100000VanZ

PF03328000100000HpcH_HpaI

PF02123000100000RdRP_4

PF00187000100000Chitin_bind_1

PF04908000100000SH3BGR

PF04324000100000Fer2_BFD

PF06964000100000Alpha-L-AF_C

PF04982000100000HPP

PF00891000100000Methyltransf_2

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