Record of Abbreviated Incoming Rodent Quarantine
University of California, San Francisco
Laboratory animal resource center
RECORD OF ABBREVIATED INCOMING RODENT QUARANTINE
Facility:______Room:______Rack:______Start Rec.: ______Stop Rec.:______
Please date & initial all entries
Week 0
_____ Start logging shipments by Investigator and shipping institution (Use attached form)
Place Fenbendazole feed in all shipment cages, (NOT sentinel cages)
_____ The business office will order sentinels before rack closes. (4-5 weeks of age): 1 cage (2 mice) per shipment. Inform the business office if you have more than 6 Investigators.
Week 1 Start Date: ______
_____ Close Quarantine rack
_____ Place Fenbendazole feed in all shipment cages, (NOT sentinel cages)
_____ Make sure every shipment has sentinels; begin dirty bedding placement in sentinel cages
_____ Treat only shipment cages, (NOT sentinel cages) with (1st dose) of a 1% solution of Ivermectin, (See dilutions, prepare fresh batch at every start) If for any reason there are pups, treat adult mice ONLY between the shoulder blades with 3uls of Moxidectin. DO NOT TREAT PUPS OR NUDE MICE!
_____ Email Rodent Health Team to give start date and list of the PI’s in the room
Week 2 ______
_____ Continue dirty bedding placement in sentinel cages
_____ Place Fenbendazole feed in all shipment cages, (NOT sentinel cages)
_____ Collect from colony mice only - fecal by PCR and body swab by PCR. Pool up to 10 samples and submit to rodent team.
Week 3 ______
_____ Continue dirty bedding placement in sentinel cages
_____ Place Fenbendazole feed in all shipment cages, (NOT sentinel cages)
_____ Treat only colony animals, (NOT sentinel cages) with (2nd dose) of a 1% solution of Ivermectin, (See dilutions, prepare fresh batch at every start) If for any reason there are pups, treat adult mice ONLY between the shoulder blades with 3uls of Moxidectin. DO NOT TREAT PUPS OR NUDE MICE!
Week 4 ______
_____ Continue dirty bedding placement in sentinel cages
_____ Place Fenbendazole feed in all shipment cages, (NOT sentinel cages)
_____ Rodent Team to perform 1st Sentinel Bleed, this will be the final bleed
Week 5 ______
_____ Place Fenbendazole feed in all shipment cages, (NOT sentinel cages)
Week 6 ______
_____ Place Fenbendazole feed in all shipment cages, (NOT sentinel cages)
RECORD OF ABBREVIATED INCOMING RODENT QUARANTINE
Facility:______Room:______Rack:______Open Date: ______End Date:______
Week 7 ______
_____ Place Fenbendazole feed in all shipment cages, (NOT sentinel cages)
Week 8 ______
_____ Place Fenbendazole feed in all shipment cages, (NOT sentinel cages)
If there is an abnormality detected, continue recording below:
Week ______
_____ Place Fenbendazole feed in all shipment cages, (NOT sentinel cages)
Week ______
_____ Place Fenbendazole feed in all shipment cages, (NOT sentinel cages)
Week ______
_____ Place Fenbendazole feed in all shipment cages, (NOT sentinel cages)
Comments (Date & Initial):Facility Supervisor: When this form has been completed, fax to the Rodent Health Team at 502-8252
DO NOT TREAT PUPS OR NUDE MICE!
IVERMECTIN FOR RODENT TREATMENT SPRAY:
- This comes in a 5% Ivermectin bottle and must be diluted to a 1.0% solution
- 200 ml 5% Ivermectin + 800 ml water
MOXIDECTIN TREATMENT FOR CAGES WITH PUPS
1. Do not treat any cages with pups under the age of 3 weeks.
2. Treat adults in these cages with topical Moxidectin - 3ul.
RECORD OF ABBREVIATED INCOMING RODENT QUARANTINE
Facility: ______Room:______Rack:______Open Date: ______End Date:______
BREEDING OR OTHER MANIPULATIONS ARE BY SPECIAL ARRANGEMENT ONLY.
MALE AND FEMALE MICE SHOULD ARRIVE & BE MAINTAINED SEPARATELY.
To: PI / From: Institution / # Males(# of Cages) / # Females (# of cages) / # Add’l Cages / # Sentinel Cages
(Note ID#)
Facility Supervisor: When IRQ is closed, fax this form to the business office at: 476-2090
Business Office: When this form has been reconciled, fax to the Rodent Health Team at 502-8252
Rev: F