Re: Submission of Manuscript, Entitled Effect of Ni (II) on Properties of Bulking Activated

Re: Submission of Manuscript, Entitled Effect of Ni (II) on Properties of Bulking Activated

Re: Submission of manuscript, entitled “The effect of continuous exposure of copper on the properties and extracellular polymeric substances (EPS) of bulking activated sludge”, forEnvironmental Science and pollution Research

Jia Lia, Xiang Liua*, Yan Liua*,Juliana Ramsayb, Chenchao Yaoa, Ruihua Daia

aDepartment of Environmental Science and Engineering, FudanUniversity, 220 Handan Road, Shanghai 200433, China

bChemical Engineering, Queen’s University, Kingston, Ontario, CanadaK7L 3N6

Corresponding author phone:86-21-6564-2018; fax: 86-21-6564-3597;

Email: .

Dear Sir or Madam:

Attached please find the original manuscript, entitled“The effect of continuous exposure of copper on the properties and extracellular polymeric substances (EPS) of bulking activated sludge”, for your consideration to be published in Environmental Science and pollution Research.All the authors mutually agree that it should be submitted to Water Research. We promise that it is the original work of the authors.

This research work presents the change on the properties and extracellular polymeric substances (EPS) of bulking activated sludge whencontinuously exposed to 20mg/L copper.The recovery of an activated sludge system to a single exposure to a toxic concentration of Cu (II) has been previously studied.However, this is the first study to investigate the recovery of an activated sludge system after 160 days of continuous exposure to Cu (II). Initially, the continuous addition of 20 mg/L Cu (II) seriously inhibited the removal of sodium benzoate (provided as a model organic pollutant)by activated sludgein a sequencing batch reactor (SBR). After 40 days of acclimation, the removal efficiency showed a slight but unsteady recovery and the settling and dewatering properties improved, indicating that sludge bulking had been inhibited. The proportion of loosely bound extracellular polymeric substances (EPS) in the total EPS increased with time andthe relative composition of the total organics waspolysaccharide > humic acid> protein> DNA. The effects of copper onthe composition of EPS, the settling and dewatering properties of bulking activated sludge were also discussedfor the first time in this paper.

This article had 3985 words including3758words in title, abstract, text, acknowledgement and referencewords contributed by6figures and 1 table.

We’d like to recommend three referees to review this paper according to requirements. Their current e-mail addresses and mailing addresses are as follows:

  1. Prof. J.H. Tay

Division of Environmental & Water Resources Engineering, Institute of Environmental Science & Engineering, NanyangTechnologyUniversity, Nanyang Avenue, Singapore639798. Tel.: 65-6790-5308; Fax: 65-6791-0676

E-mail:

  1. Jeff Kuo

Department of Civil & Engineering, CaliforniaStateUniversity, Fullerton. CA92834, USA

Tel: 657-278-3995, Fax: 657-278-3995

Email:

  1. Yin-guang Chen

State Key Laboratory of Pollution Control and Resources Reuse, School of Environmental Science and Engineering, Tongji University, 1239 Siping Road, Shanghai 200092, PR China

Tel.: +86 21 65981263; fax: +86 21 65986313.

E-mail:

We hope that you will find this manuscript worthy of publication, and look forward to hearing from you soon.

Sincerely yours,

Dr.XIANG LIU

Prof. YANLIU

CC: Miss Jia Li

Prof. Juliana A. Ramsay

Mr. Chenchao Yao

Dr. Ruihua Dai

Title:The effect of continuous exposure of copper on the properties and extracellular polymeric substances (EPS) of bulking activated sludge

Orders of Authors: Jia Lia, Xiang Liua*, Yan Liua*, Juliana Ramsayb, Chenchao Yaoa, Ruihua Daia,

Corresponding Author:Xiang Liu, Yan Liu

Contact information:

Jia Li

Department of Environmental Science and Engineering, FudanUniversity, Shanghai, 200433, China

Telephone: +86-55664403

Email address:

Xiang Liu

Department of Environmental Science and Engineering, FudanUniversity, Shanghai, 200433, China

Telephone: +86-21-65642018

Email address:

Yan Liu

Department of Environmental Science and Engineering, FudanUniversity, Shanghai, 200433, China

Telephone: 86-21-6564-3348; fax: 86-21-6564-3597

Email address:

J. A.Ramsay

Chemical Engineering, Queen’s University, Kingston, Ontario, CanadaK7L 3N6

Fax: +613-533-6637;

Email address:

Chenchao Yao

Department of Environmental Science and Engineering, FudanUniversity, Shanghai, 200433, China

Telephone: +86-55664403

Email address:

Ruihua Dai

Department of Environmental Science and Engineering, FudanUniversity, Shanghai, 200433, China

Telephone: 86-21-6564-4244; fax: 86-21-6564-3597

Email address: