Rasgrf2 controls noradrenergic involvement in the acute and subchronic effects of alcohol in the brain

Alanna C. Easton1*, Andrea Rotter2*, Anbarasu Lourdusamy1*, Sylvane Desrivières1, Alberto Fernández-Medarde3, Teresa Biermann2, Cathy Fernandes1, Eugenio Santos3, Johannes Kornhuber2, Gunter Schumann1, Christian P. Müller1,2

Supplement material


Supplement Tab. 1 Co-expression of Rasgrf2 and serotonergic pathway mRNA in the ventral striatum of wild type mice.

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Gene R p

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Htr1a / 0,6829 / 0,7675
Bdnf / 0,6768 / 0,8147
Htr1f / 0,5445 / 1,0000
Maoa / -0,5066 / 1,0000
Htr2a / 0,4892 / 1,0000
Htr3b / -0,4094 / 1,0000
Htr2c / 0,4023 / 1,0000
Htr1b / -0,3950 / 1,0000
Htr3a / 0,3476 / 1,0000
Htr4 / 0,3133 / 1,0000
Htr1d / 0,2982 / 1,0000
Tph2 / 0,2886 / 1,0000
Htr6 / -0,2323 / 1,0000
Slc6a4 / 0,2313 / 1,0000
Htr7 / -0,1423 / 1,0000
Htr5a / 0,1259 / 1,0000
Tph1 / 0,0855 / 1,0000
Htr2b / -0,0396 / 1,0000

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Supplement Tab. 2 The role of Rasgrf2 in acute alcohol effects on locomotor activity. Raw values (mean of 20 min interval) of locomotor activity before (time intervals 1-3) and after an alcohol (2 g/kg, i.p.) injection in Rasgrf2-/- and WT mice.

Time
Interval / WT +/+
Mean / SEM / Ras -/-
Mean / SEM
1 / 165.90 / 72.64 / 438.62 / 182.07
2 / 71.73 / 47.18 / 345.55 / 184.55
3 / 70.75 / 24.23 / 462.37 / 241.00
4 / 28.10 / 16.02 / 115.82 / 42.78
5 / 35.32 / 26.28 / 26.65 / 22.28
6 / 36.83 / 24.83 / 41.97 / 38.44
7 / 22.93 / 14.27 / 141.20 / 80.32
8 / 194.32 / 148.93 / 268.77 / 125.41
9 / 238.88 / 195.07 / 242.67 / 122.28
10 / 360.78 / 211.21 / 349.33 / 202.54
11 / 427.55 / 255.58 / 304.45 / 106.16
12 / 340.27 / 200.60 / 205.02 / 130.73
13 / 520.45 / 281.18 / 447.52 / 260.93