农学院2009年共发表SCI论文130篇，详细情况如下：
1.
【篇名】Purification of a bioactive recombinant human Reg IV expressed in Escherichia coli
【作者】Hu, G. Y.,Shen, J. Q.,Cheng, L.,Xiang, D.,Zhang, Z. H.,He, M.,Lu, H. L.,Zhu, S. Y.,Wu, M. Y.,Yu, Y.,Wang, X. P.,Han, W.
【期刊】Protein Expr. Purif.,69(2)
【摘要】Regenerating gene (Reg) IV is a newly discovered member of the regenerating gene family belonging to the calcium (C-type) dependent lectin superfamily. Reg IV is highly expressed in the gastrointestinal tract and markedly up-regulated in colon adenocarcinoma, pancreatic cancer, gastric adenocarcinoma, and inflammatory bowel disease. However, the physiological and pathological functions of Reg IV are largely unknown, partly due to the limited access of the bioactive protein. We report here the first expression and purification of Reg IV proteins using a prokaryotic system. Human Reg IV was expressed in Escherichia coli as an insoluble protein which was identified in the fraction of inclusion body after ultrasonication of the bacteria. After the protein aggregate was solubilized by guanidine-HCl, it was refolded by sucrose and arginine-assisted procedures and purified using cation-exchange chromatography. The protein identity and purity of the final preparation were confirmed by analysis of the protein mass and immune specificity in SDS-PAGE, Western blotting, and HPLC assay. The biological activity of the protein was determined by the HCT116 and HT29 cell proliferation assays. The highly purified bioactive human Reg IV should aid in further characterization of its physiological and pathological functions. (C) 2009 Elsevier Inc. AM rights reserved.
2.
【篇名】YIELD, FRUIT QUALITY AND NITROGEN UPTAKE OF ORGANICALLY AND CONVENTIONALLY GROWN MUSKMELON WITH DIFFERENT INPUTS OF NITROGEN, PHOSPHORUS, AND POTASSIUM
【作者】Song, S. W.,Lehne, P.,Le, J. G.,Ge, T. D.,Huang, D. F.
【期刊】J. Plant Nutr.,33(1)
【摘要】The effects of varied amounts of fertilization on yield, fruit quality, and nitrogen (N) uptake of muskmelons (Cucumis melo L. var reticulatus Naud) grown under both organic and conventional farming conditions were evaluated. Organic fertilizer (0.0, 0.55, 1.1, and 2.2 kg m-2) and mineral fertilizers containing the same amounts of estimated plant available nutrients [N, phosphorus (P), and potassium (K)] were applied to organic and conventional farming plots, respectively, in both the spring and autumn seasons of 2005. In comparison to conventional farming conditions, muskmelons grown under organic farming conditions had the same yield, total soluble solids (TSS) and soluble sugar contents in both growing seasons, and fruit pulp nitrate content was significantly reduced by 12% on average in spring and 16% on average in autumn. At harvest maturity the aboveground plant N concentration was significantly higher in the conventional treatments than in the organic treatments. At the vine growth stage, the plant N concentrations were similar in all treatments in both seasons. The ratios of nitrate N to total N amount in aboveground biomass were higher in conventional and high fertilized organic treatments than in low or not fertilized organic treatments under limited N supply from the soil. Muskmelon plants absorbed mainly inorganic N, and the protein N fraction in the xylem sap was larger than the amino acid N fraction. Plants grown in the organic system had a higher proportion of organic N in their xylem sap, especially when manure input was low.
3.
【篇名】Expressions and purification of a mature form of recombinant human Chemerin in Escherichia coli
【作者】Xiang, D.,Zhang, J.,Chen, Y. Z.,Guo, Y. P.,Schalow, A.,Zhang, Z. H.,Hu, X. J.,Yu, H. J.,Zhao, M.,Zhu, S. Y.,Lu, H. L.,Wu, M. Y.,Yu, Y.,Moldenhauer, A.,Han, W.
【期刊】Protein Expr. Purif.,69(2)
【摘要】Chemerin is a novel chemokine that binds to the G protein-coupled receptor (GPCR) ChemR23, also known as chemokine-like receptor 1 (CMKLR1). It is secreted as a precursor and executes pro-inflammatory functions when the last six amino acids are removed from its C-terminus by serine proteases. After maturation, Chemerin attracts dendritic cells and macrophages through binding to ChemR23. We report a new method for expression and purification of mature recombinant human Chemerin (rhChemerin) using a prokaryotic system. After being expressed in bacteria, rhChemerin in inclusion bodies was denatured using 6 M guanidine chloride. Soluble rhChemerin was prepared by the protein-specific renaturation solution under defined conditions. It was subsequently purified using ion-exchange columns to more than 95% purity with endotoxin level <1.0 EU/mu g. We further demonstrated its biological activities for attracting migration of human dendritic cells and murine macrophages in vitro using established chemotaxis assays. (C) 2009 Elsevier Inc. All rights reserved.
4.
【篇名】Study on structural changes of microwave heat-moisture treated resistant Canna edulis Ker starch during digestion in vitro
【作者】Zhang, J.,Chen, F.,Liu, F.,Wang, Z. W.
【期刊】Food Hydrocolloids,24(1)
【摘要】Microwave heat-moisture treated Canna edulis Ker starch has been applied to study structural changes in vitro using porcine a-amylase, pancreatin and amyloglucosidase. The structures at different digestion stages were characterized using scanning electron microscopy (SEM), polarized optical microscopy (POM), small angle X-ray scattering (SAXS), X-ray diffraction (XRD), Fourier transforms infrared (FT-IR) spectroscopy and solid state C-13 nuclear magnetic resonance (NMR) spectroscopy. The increase in molecular order was observed with increasing digestion time and reflected in higher scattering intensity measured by SAXS, higher crystallinity observed by XRD, the increase of the double helix order obtained by solid state C-13 NMR spectroscopy, and corresponding changes in the measurement of FT-IR, in favour of increasing resistant starch content. SAXS reveals a single peak around 0.6335 nm(-1), suggesting that the enzyme may erode the special site of granule surface and catalyze whole hydrolysis reaction through pitted canals. In the process of digestion, amorphous region of starch granule is susceptible to the attack of enzyme. Moreover, the result also demonstrates that the resistance to enzymatic digestion may mainly depend on specific structure in the treated starches. (C) 2009 Elsevier Ltd. All rights reserved.
5.
【篇名】State of art of biomass fast pyrolysis for bio-oil in China: a review
【作者】Deng, C. J.,Liu, R. H.,Cai, J. M.
【期刊】J. Energy Inst.,2008, 81(4)
【摘要】This paper presents a review of biomass fast pyrolysis for the production of bio-oil in Mainland China. The main contents are as follows. The feedstock for fast pyrolysis and main pyrolysis reactor developed in Mainland China are introduced. The process of fast pyrolysis for each pyrolysis reactor mentioned in this paper is described. The effects of key parameters of fast pyrolysis on fluidised bed reactor are illustrated. Finally, the properties, upgrading and application of bio-oil are discussed.
6.
【篇名】Monitoring NH3, N2O, CO2 and CH4 Emissions During Pig Solid Manure Storage - Effect of Turning
【作者】Hassouna, M.,Espagnol, S.,Robin, P.,Paillat, J. M.,Levasseur, P.,Li, Y.
【期刊】Compost Sci. Util.,2008, 16(4)
【摘要】In France, as in other countries, there is a need to improve knowledge of greenhouse gas and ammonia emissions from livestock production with various management options. The aim of this study was to quantify the NH3, N2O, CO2 and CH4 emissions from two pig solid manure heaps, turned and not turned, in order to examine the effect of turning on gaseous emissions and to obtain additional information about emissions during composting in commercial conditions. The manure produced from the fattening of 72 pigs on straw bedding was divided between two identical heaps and stored outside on a concrete area for three months during autumn 2004. One heap was turned at day 0, 11 and 27. Gaseous emissions (NH3, N2O, CH4, CO2, H2O) of both heaps, covered for the purpose by ventilated greenhouses, were continuously measured over four periods (57 days in total). The tracer method, using SF6, was used to measure the ventilation rate in the greenhouses. Concentrations inside and outside the greenhouses (to have a differential) were measured by photoacoustic infrared absorption spectrometry with a gas analyser coupled to a multipoint sampler and closer. Emissions were calculated by combining various flows and differential concentrations and interpolated between the different measuring periods. Results show that emission kinetics were very similar for the two heaps, except after turning, when emissions are given a new impetus. On average, the measured gas emissions for unturned and turned heaps represent a nitrogen loss (mainly in the form of NH3) of 10.5% and 9% of the initial nitrogen, and a carbon loss (mainly in the form of CO2) of 24 et 28% of the initial carbon. For both heaps, N2O is the primary greenhouse gas emitted (3% of the initial nitrogen) and not CO2 or CH4. Considering the uncertainty on the measurements and the representativity of the experiment, these results did not show any clear difference between the two heaps. They underlined the relationship between gaseous emissions and the initial product (dry matter > 30%) and the diversity of the litters, which will be studied on the farm.
7.
【篇名】Optimization of Induction and Culture Conditions and Tropane Alkaloid Production in Hairy Roots of Anisodus acutangulus
【作者】Li, L.,Wang, J.,Wang, W.,Lu, Y.,Wang, Y. L.,Zhou, G. Y.,Kai, G. Y.
【期刊】Biotechnol. BioprocessEng.,2008, 13(5)
【摘要】In this study, an efficient transformation system for the medicinal plant Anisodus acutangulus was successfully developed and optimized using Agrobacterium rhizogenes. Three bacterial strains, A4, R1601, and modified C58C1 and three explant types, leaf blade, petiole, and stem, were examined. The highest transformation efficiency of 94.44% was achieved using strain C58C1 with stem explants. Over 20 independent hairy root lines were successfully established with strain C58C1 using stem explants, all of which contained the ro/B and ro/C genes as confirmed by polymerase chain reaction (PCR). Out of four media compositions, the liquid 1/2 MS medium was found the most suitable for hairy root growth. The maximum bio-mass of one hairy root line increased up to 80 times in liquid 1/2 MS medium after a 30 day culture period. Different hairy root lines displayed a varied capacity for tropane alkaloid production and the best hairy root line (T4) from the C58C1-stem combination produced up to 10.21 mg/g (dw) of hyoscyamine, which was about 1.5-fold higher than in the wild type plants. To our knowledge, this is the first report to demonstrate the production of tropane alkaloids in hairy roots of A. acutangulus. (C) KSBB
8.
【篇名】Detecting QTLs for plant architecture traits in cucumber (Cucumis sativus L.)
【作者】Li, X. Z.,Yuan, X. J.,Jiang, S.,Pan, J. S.,Deng, S. L.,Wang, G.,Le He, H.,Wu, A. Z.,Zhu, L. H.,Koba, T.,Cai, R.
【期刊】Breed. Sci.,2008, 58(4)
【摘要】To improve the efficiency of breeding Cucumber ill China, we previously mapped QTL for most fruit- and flower-related traits of this species. Here, we mapped QTLs for six plant architecture traits including lateral branch number (LBN), lateral branch total length (LBTL), main-stern length (MSL), internode length (TNL), main-stem diameter (MSD), and petiole length (PL) were detected in greenhouse environments. In total, 14 QTLs were identified for the six traits (LBN, 3; LBTL, 2; MSL, 3; INL, 2; MSD, 2; and PL, 2) with additive heritability of individual QTL, ranging from 1.6% to 29.5%. Five QTLs for four traits (LBN, LBTL, MSL, and INL) were observed to have significant (P <= 0.05) QTL x environment interaction effects. The broad-sense heritability for the six traits ranged from 8.5% to 47.0%. The QTL information presented in this research, together with the data in Our previous study on the fruit- and flower-related traits, will facilitate the breeding of elite cucumber cultivars by marker-assisted selection in China.
9.
【篇名】A Preliminary Study of Eperythrozoonsis in Shanghai
【作者】Liang, A. B.,Yao, C. B.,Hua, X. G.,Yuan, C. L.
【期刊】Blood,2008, 112(11)
【摘要】
10.
【篇名】Isolation and molecular characterization of a cax gene from Capsella bursa-pastoris
【作者】Lin, J.,Zhang, W.,Shi, M. Z.,Wang, X. L.,Sun, X. F.,Tang, K. X.
【期刊】Biocell,2008, 32(3)
【摘要】A new cation exchangers (CAXs) gene was cloned and characterized from Capsella bursa-pastoris by rapid amplification of cDNA ends (RACE). The full-length cDNA sequence of cax from C. bursa-pastoris (designated as Cbenx51) was 1754 by containing a 1398 by open reading frame encoding a polypeptide of 466 amino-acid residues with a calculated molecular mass of 50.5 kDa and an isoelectric point of 5.69. The predicted CbCAX51 contained an IMP dehydrogenase/GMP reductase domain, two Na+/Ca+ exchanger protein domains. Comparative and bioinformatics analyses revealed that CbCAX51 showed extensive homology with CAX from other plant species. The expression analysis by different treatments indicated that Cbcax51 could be activated by cold triggering and was related to the cold acclimation process, but its expression is regulated negatively by drought and not affected by ABA or salt.
11.
【篇名】Molecular cloning, characterization and expression of a jasmonate biosynthetic pathway gene encoding allene oxide cyclase from Camptotheca acuminata
【作者】Pi, Y.,Liao, Z. H.,Jiang, K. J.,Huang, B. B.,Deng, Z. X.,Zhao, D. L.,Zeng, H. N.,Sun, X. F.,Tang, K. X.
【期刊】Biosci. Rep.,2008, 28(6)
【摘要】AOC (allene oxide cyclase; EC 5.3.99.6), an essential enzyme in jasmonic acid and its methyl ester biosynthesis, was cloned from Camptotheca acuminata (named as CaAOC), a native medicinal plant species in China. CaAOC had significant similarity at the amino-acid level with AOCs from other plant species. Comparison between the sequences of the full-length cDNA and genomic DNA of CaAOC revealed that the genomic DNA of CaAOC contained an 89-bp intron and a 240-bp intron. Southern-blot analysis indicated that CaAOC was a multiple-copy gene, and real-time quantitative PCR analysis showed that CaAOC was expressed constitutively in all organs tested, with the highest expression level in leaves. The results from treatment experiments using different signalling components, including methyl jasmonate, abscisic acid, salicylic acid and H2O2, revealed that expression of CaAOC had a prominent diversity. Heavy metal (copper) significantly enhanced CaAOC expression, whereas wounding (induced by UV-B) was not so effective.
12.
【篇名】Influence of dehydration on the desert moss in molecular mobility and membrane fluidity monitored by spin label
【作者】Shu, S. J.,Wang, Y.,Chen, W. H.,Wang, Z. W.
【期刊】Biochem. Syst. Ecol.,2008, 36(12)
【摘要】
13.
【篇名】Epidemiology of hepatitis E
【作者】Zhu, J. G.,Hua, X. G.,Zhang, Y. L.,Yang, Z. B.
【期刊】Rev. Med. Microbiol.,2008, 19(4)
【摘要】Hepatitis, caused by hepatitis E virus (HEV) infection, has been regarded a serious public health problem in developing countries. However, recent studies indicate that HEV also circulates in industrialized countries. This review summarizes the current knowledge of epidemiology of hepatitis E. Several studies have shown that there exists a possibility of interspecies transmission between the human and other reservoirs; hepatitis E is becoming a concern in countries where HEV is not, traditionally, believed to be endemic. Progress has also been made to analyze genotype and genetic diversity of HEV isolates from different geographic regions to trace the ancestors of HEV, to indicate the environmental sources and routes of transmission of HEV, and to investigate epidemiology of hepatitis E infections by demographic features. This might provide the essential understanding of the epidemiology of infectious diseases. (C) 2009 Wolters Kluwer Health vertical bar Lippincott Williams & Wilkins
14.
【篇名】Kinetic Analysis of Wheat Straw Oxidative Pyrolysis Using Thermogravimetric Analysis: Statistical Description and Isoconversional Kinetic Analysis
【作者】Cai, J. M.,Alimujiang, S.
【期刊】Ind. Eng. Chem. Res.,2009, 48(2)
【摘要】The oxidative pyrolysis kinetics of wheat straw was studied by thermogravimetric analysis (TGA) under oxygen dynamic atmosphere. Nonisothermal TGA data at three heating rates of 5, 10, and 20 K min(-1) were analyzed. The kinetic conversion data calculated from the TGA data were fitted by the Weibull and logistic mixture models, and the corresponding statistical analyses were performed. The statistical results showed that the Weibull mixture model fitted the experimental data better than the logistic mixture model and can accurately reproduce the kinetic conversion data. Making use of the data predicted by the Weibull mixture model, some data required in the isoconversional kinetic analysis, such as alpha vs T-alpha, can be easily obtained. An iterative linear integral isoconversional method was developed and applied to evaluate the activation energy of the oxidative pyrolysis process of wheat straw. The Vyazovkin-Dollimore nonlinear integral isoconversional method was also used to calculate the activation energy. The results have shown that two isoconversional methods are equivalent for the estimation of the activation energy and the obtained activation energy is significantly dependent upon conversion for the oxidative pyrolysis process of wheat straw.
15.
【篇名】Kinetic analysis of wheat straw pyrolysis using isoconversional methods
【作者】Cai, J. M.,Bi, L. S.
【期刊】J. Therm. Anal. Calorim.,2009, 98(1)
【摘要】The pyrolysis of wheat straw has been carried out by means of thermogravimetric analysis in inert atmosphere. The samples were heated over a range of temperatures that includes the entire range of pyrolysis with three different heating rates of 5, 10 and 20 K min(-1). The activation energy values as a function of the extent of conversion for the pyrolysis process of wheat straw have been calculated by means of the Flynn-Wall-Ozawa isoconversional method, the Vyazovkin-Sbirrazzuoli isoconversional method and an iterative isoconversional method presented in this article. The results have showed that there are small differences between the activation energy values obtained from the three methods, and the pyrolysis process reveals a dependence of the activation energy on conversion and have indicated the validity of the iterative integral isoconversional method. The effective activation energy for the pyrolysis of wheat straw is 130-175 kJ mol(-1) in the conversion range of 0.15-0.85. Furthermore, the prediction of the pyrolysis process under isothermal conditions from the dependence of the activation energy on the extent of conversion has been presented.
16.
【篇名】A New Iterative Linear Integral Isoconversional Method for the Determination of the Activation Energy Varying with the Conversion Degree
【作者】Cai, J. M.,Chen, S. Y.
【期刊】J. Comput. Chem.,2009, 30(13)
【摘要】The conventional linear integral isoconversional methods may lead to important errors in the determination of the activation energy when the significant variation of the activation energy with the conversion degree occurs. Vyazovkin proposed all advanced nonlinear isoconversional method, which allows the activation energy to be accurately determined [Vyazovkin, J Comput Client 2001. 22, 178]. However, the use of the Vyazovkin method raises the problem of the time-consuming minimization without derivatives. A new iterative linear integral isoconversional method for the determination of the activation energy as a function of the conversion degree has been proposed, which is capable of providing valid values of the activation energy even if the latter strongly varies with the conversion degree. Also, the new method leads to the correct values of the activation energy in much less time than the Vyazovkin method. The application of the new Method is illustrated by processing of theoretically simulated data of a strongly varying activation energy process. (C) 2009 Wiley Periodicals, Inc. J Comput Client 30: 1986-1991, 2009