Protein Request Form

I agree that pursuant to MJFF's agreement with Proteos, the supplier of Parkinson’s disease-relevant proteins, these proteins may be used only for research purposes, and any commercial use of these proteins requires separate written agreement with the supplier and MJFF. I agree that the proteins will not be used in humansfor the treatment or diagnosis of human disease.

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Signature
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This form must be signed and submitted to Proteos, Inc. () in order to receive the requested materials.

MJFF DJ-1 Protein Library Request Form

Please complete the request form below and return to Proteos, Inc. (). By requesting these materials, you agree to use them for research purposes only.

Investigators from non-profit and for-profit entities may request aliquots of any of the Parkinson’s disease-relevant proteins, generated with support from The Michael J. Fox Foundation for Parkinson’s Research:

PRINCIPAL INVESTIGATOR
INSTITUTE/COMPANY
REQUESTOR AFFILIATION
(Non-Profit/For-Profit)
EMAIL
PHONE NUMBER
LAB CONTACT FOR SHIPPING
(If different from PI)
LAB CONTACT -- EMAIL
LAB CONTACT –
PHONE NUMBER
SHIPPING ADDRESS
Preferred FREIGHT CARRIER and Account #
MJFF PROTEINS AVAILABLE / Protein / Description
human DJ-1 (E. coli) / Recombinant human DJ-1 was expressed in E. coli. The protein was purified by size exclusion chromatography and ion exchange chromatography.
His tagged human DJ-1 (E. coli) / Recombinant His-tagged human DJ-1 was expressed in E. coli. The protein was purified by affinity and size exclusion chromatography
His-tagged human DJ-1 (HEK) / Recombinant human DJ-1 was expressed in 293-6E cells with an N-terminal His-tag. The protein was purified by affinity chromatography and size exclusion chromatography.
TEV cleaved (untagged) human DJ-1 (HEK) / Recombinant human DJ-1 was expressed in 293-6E cells with an N-terminal His-tag. The protein was purified by affinity chromatography, His-tag removed via TEV protease and cleaved protein was further purified by size exclusion chromatography.
Oxidized DJ-1 (E. coli) / Recombinant human DJ-1 was expressed in E. coli with50 mMTris, 150mM NaCl, 20 mM reduced glutathione, pH 8.0 to produce an oxidized DJ-1 peptide.
QUANTITIES OF MJFF PROTEINS BEING REQUESTED / Please indicate the number of aliquotsrequested in the table below (limit 2 aliquots per protein).
Requests above 2aliquots total per protein in a single order will not be considered. Questions about this limit can be addressed to
Protein / aliquot size / # of aliquots requested
human DJ-1 (E. coli) / 1 mg
His tagged human DJ-1 (E. coli) / 1 mg
His-tagged human DJ-1 (HEK) / 1.6 mg
TEV cleaved (untagged) human DJ-1 (HEK) / 1.05 mg
Oxidized DJ-1 (E. coli) / 1 mg

This form must be submitted to Proteos, Inc ()in order to receive the requested materials.

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