PROFESSOR: DEAN JOHNSTON OFFICE: E027
OFFICE HOURS: T 1:00 - 3:00W 12:00-1:30, PHONE: 481-4356 or 263-5618
Th 11:00 - 1:00
email:
DATE LABORATORY
Aug 28 Introduction to the lab and Contamination of Cultures
Sept 4 Preparation of tissue culture media
Sept 11 Quantitative cellular measures
Sept 25 Passage, freezing, and maintaining cells
Oct 2 Thawing cells and serum requirements
Oct 16 Cell fractionation and enzymatic activity
Oct 23 Growth factors
Oct 30 Cell cycle studies
Nov 6 in vivo passage of tumor cells part I: inoculation of mice
Nov 13 Primary murine fibroblasts
Nov 20 Monoclonal antibodies
Nov 27 in vivo passage of tumor cells part II: isolation of cells
Dec 4 Chromosomal analysis
Dec 11 ELISA
ATTENDANCE POLICY:
Students are responsible for all work in this course
No unexcused absence or tardiness will be permitted
Attendance will be taken at the beginning of the class
In this course the labs cannot be made up. For any excused absence you must contact me before the next class to arrange for an alternate assignment.
Course Web Page
Cell & Tissue Culture will have an informational file on the Hunter College computer system. To access this file go to the Hunter home page (hunter.cuny.edu). At the home page click on Student Services then on Course Web Pages. From here search by course number for 450. Click on MLS 450. This course is code protected. The code is mls450.