Supplemental Table S2:
Primer sequences and amplicon characteristics.
Gene / Primer sequences (5´-3´) / Amplicon length (bp) ofcDNA and gDNA / PCR efficiency (%) / R2 / Tm (Cº)
VcCRYa / F: CCAATTTCCACCGCATGGATG
R: AGTCATGATGGCGTCGATCC / 125 / 387 / 99.8 / 0.991 / 87
VcCRYp / F: CAGACTGCAGTGGTTTGGTTC
R: GAACTGTCCTTCTTCCTCAGG / 129 / 435 / 99.8 / 0.993 / 85.5
VcCRYd1 / F: GTCCACATACTGGGTCATATTCG
R: ATCTGGATCCGGATTCCAAACC / 133 / 390 / 99.8 / 0.986 / 84
VcCRYd2 / F: TCACCGGCTGGATGAGCAAC
R: TGCAATAGTTAACCGCCACATC / 131 / 326 / 99.8 / 0.997 / 86.5
VcPhot / F: CGTGCGAACAGTTTCGTGG
R: GTAGTGCCGTACAGCAGCTC / 128 / 500 / 99.7 / 0.992 / 87
VR1 / F: CTGAAGAACTTGGGAGCTGAG
R: CCATCGACCTCCACGTTAAG / 137 / 208 / 99.8 / 0.990 / 84
VChR1 / F: CAATTGTTTTCTCAATGCTGGTACC
R: TCCACTATGGATTATCCCGTTGC / 145 / 291 / 99.8 / 0.994 / 85.5
VChR2 / F: AAGAATGGACCACCCAGTTGC
R: GTGAACCCTTGGACTTAAGCC / 125 / 199 / 99.7 / 0.988 / 83
VcHKR1 / F: CACGGTTATGTGGTTGCTCAC
R: TACCGAAGGAGATCATCAACACG / 122 / 213 / 99.7 / 0.994 / 83
VcHKR2 / F: GGATCCAACGACTTTGTACGG
R: GTCCACATTGTTAACCAACTCAGC / 120 / 448 / 99.8 / 0.994 / 85
VcHKR3 / F: CTTCCTGAAGGATGCCATGAC
R: GGATACATCGCTGGAGAGG / 103 / 252 / 99.6 / 0.989 / 80.5
VcHKR4 / F: CACCACTGAACGGAATCATCG
R: CGCTTACCAGACAGTTTGACC / 112 / 443 / 99.8 / 0.995 / 84
18S / F: GATACCGTCGTAGTCTCAACC
R:TTCAGCCTTGCGACCATACTC / 131 / 131 / 99.7 / 0.987 / 82.5
eef1 / F: CAAGGTGCATCTCTCCATCG
R:TTCTCCAGCTCACGCTCAG / 111 / 284 / 99.8 / 0.988 / 87
rlp23 / F: CGAAGACCTTGAAGCGGAC
R:TGTCCTCAATCTTCTTCATTGCC / 132 / 668 / 99.8 / 0.993 / 86
tbpA / F: GTAGTGGCTACTGTGAATCTGG
R:GCTCTCTAATACGCATAATGACG / 118 / 208 / 99.8 / 0.986 / 83.5
F: forward primer, R: reverse primer, gDNA: genomic DNA, cDNA: complementary DNA, R2: regression coefficient, Tm: melting temperature.