Lining Guo, Ph.D.

Work Address

Metabolon Inc

800 Capitola Drive, Suite 1

Durham, NC 27713

Email:

Phone: 919-287-3356

Fax: 919-572-1721

Professional Experience

2006 – present Head of Commercial Operation/Senior Director, Metabolon, Inc., Durham, NC

  • Responsible for contract research operation in Metabolon, a world leader in metabolomics technology. Successfully completed over 400 projects for pharmaceutical, biotech and consumer product companies in the last three years.
  • Oversee ~ 100 client accounts consisting of top 15 pharmaceutical companies in the world, all the major biotech companies in US and Europe, all the major pharmaceutical companies in Japan, consumer product companies such as Nestle and Colgate, and many research foundations.
  • Establish organizational and project management policies and guidance. Prioritize projects and ensure consistency of projects with corporate strategy and profitability.
  • Established and assembled a team of appropriate functional experts and staff (analytical chemists, biochemists and statisticians), and coaching to achieve success.
  • Participated business development that directly contributed to > US$ 12 million contracts.
  • Provided guidance to continued metabolomic technology platform improvement.

2000 –2005Director of Biochemistry and HTS Assay Development, Icoria/Paradigm Genetics, Inc., RTP, North Carolina

  • Responsible foraUS $35 million research contract focused on functional genomics and HTS assay developmentbetween Paradigm Genetics and Bayer Cropsciences.
  • Responsible for development of over 45 fully integrated novel HTS assays (enzymatic, receptor binding and cell based)and implemented the assay in Bayer’s HTS screening facility in Monheim, Germany.
  • Established a chemical synthesis and protein production facility to supply all the reagents necessary for Bayer’s compound library screening activities in Monheim, Germany.
  • Assembled and managed a team of experts of biochemists, chemists and molecular biologists to consistently met project milestones and financial projections.
  • Developed various immunoassays for external collaborators.

1995 – 2000Biochemistry Technical Leader (02/97 - 05/00); Research Biochemist (02/95 – 01/97), Dow AgroSciences LLC (a Dow Chemical Company), Indianapolis, Indiana

  • Supervising and providing leadership to a staff of 15 scientists and research associates and responsible for recruiting, training and motivating of the same.
  • Communicates effectively with upper management and with potential collaborators, and identifies new opportunities.
  • Leading the project team to develop objectives, strategy, budget and tactical plans for the implementation of various research projects.
  • Discovered, purified, and characterized a new class of insecticidal toxins from Photorhabdus and Xenorhabdus bacteria.
  • Purified and characterized over 20 different enzymes and proteins for various project teams.
  • Among inventors for six issued patents.

1993 – 1994Postdoctoral scientist, The Samuel Roberts Noble Foundation, Ardmore, Oklahoma

  • Discovered that two new enzymes, named vestitione reductase and DMI dehydratase, catalyzed two essential steps in secondary metabolism;
  • Purified and characterized vestitione reductase and DMI dehydratase and presented evidences that the protein-protein interactions between the two enzymes were important for efficient biosynthesis.
  • Provided key evidences on how different pterocarpan compounds with different stereochemistry were synthesized in plants.
  • Cloned the vestitone reductase gene and studied the gene regulation under different conditions.

Education

Ph. D in Plant Physiology, The PennsylvaniaStateUniversity, 1993

B. S. in Botany, Nanjing University, China, 1987

Awards

Dow AgroSciences Discovery Special Recognition Award: 1995

Dow AgroSciences Inventor Award: 1995

Dow AgroSciences Inventor Award: 1996

Dow AgroSciences Discovery Research Award: 1996

Dow AgroSciences Inventor Award: 1997

Dow AgroSciences Inventor Award: 1998

Dow AgroSciences Discovery Research Award: 1998

Dow AgroSciences Inventor Award: 1999

Publications

Tetsuya Ohta, Naoya Masutomi, Naohisa Tsutsui, Tetsuya Sakairi, Matthew Mitchell, Michael V. Milburn, John A. Ryals, Kirk D. Beebe and Lining Guo* (2009) Untargeted Metabolomic Profiling as An Evaluative Tool of Fenofibrate-induced Toxicology in Fischer 344 Male Rats. Toxicologic Pathology, 37(4):521-35. (* corresponding author)

Virginia M Barnes, Ricardo Teles, Harsh M Trivedi, William Devizio, Tao Xu, Matthew W Mitchell, Michael V Milburn, and Lining Guo* (2009) Acceleration of purine degradation by periodontal diseases. Journal of Dental Research, 88(9):851-855. (* corresponding author)

Scott E. Sattler, Jaswinder Singh, Eric Haas, Lining Guo, Gautam Sarath, Robert A. Graybosch, Peggy G. Lemaux and Jeffrey F. Pedersen (2009) Two distinct waxy alleles impact the Granule-Bound Starch Synthase in sorghum. Molecular Breeding, in press

Kay A. Lawton, Kirk Beebe, Alvin Berger, Lining Guo, Don Rose, Anne Roulston, Naohisa Tsutsui, John A. Ryals, and Michael V. Milburn (2009) Technology and application of metabolomics: comprehensive biochemical profiling to solve complex biological problems. In “Computational and Systems Biology. Methods and Applications”, R Mazzarella and R Head eds, Research Signpost, ISBN 978-81-308-0298-5, p115-140

Lawton, K. A., Berger, A., Mitchell, M., Milgram, K. E., Evans, A. M., Guo, L., Hanson, R. W., Kalhan, S. C., Ryals, J. A., and Milburn, M. V. (2008). Analysis of the adult human plasma metabolome. Pharmacogenomics 9, 383-97.

Guo, Lining; Fatig, Raymond O., III; Orr, Gregory L.; Schafer, Barry W.; Strickland, James A.; Sukhapinda, Kitisri; Woodsworth, Aaron T.; Petell, James K. (1999) Photorhabdus Luminescens W-14 insecticidal activity consists of at least two similar but distinct proteins. Purification and characterization of toxin A and toxin B. Journal of Biological Chemistry, 274(14), 9836-42

Guo, Lining; Paiva, Nancy L (1995). Molecular cloning and expression of alfalfa (Medicago sativa L) vestitone reductase, the penultimate enzyme in medicarpin biosynthesis. Archives in Biochemistry and Biophysics, 320(2), 353-60

Guo, Lining, Dixon, Richard A.; Paiva, Nancy L. (1994) The ‘pterocarpan synthase’ of alfalfa: association and co-induction of vestitone reductase and 7,2’-dihydroxy-4’-methoxy-isoflavanol (DMI) dehydratase, the two final enzymes in medicarpin biosynthesis. FEBS Letter, 356(2,3), 221-5

Guo, Lining; Dixon, Richard A.; Paiva, Nancy L. (1994) Conversion of vestitone to medicarpin in alfalfa (Medicago sativa L) is catalyzed by two independent enzymes: identification, purification, and characterization of vestitone reductase and 7,2’-dihydroxy-4’-methoxyisoflavanol (DMI) dehydratase. Journal of Biological Chemistry, 269(35): 22372-8

Guo, Lining; Phillips, Allen T.; Arteca, Richard N. Amino acid N-malonyltransferases from mung bean: action on 1-aminocyclopropane-1-carboxylic acid and D-phenylalanine. Journal of Biological Chemistry (1993) 268(34), 25389-94

Guo, Lining, Arteca, Richard N.; Phillips, Allen T.; Liu, Yu. Purification and Characterization of 1-aminocyclopropane-1-carboxylate N-malonyltransferase from etiolated mung bean hypocotyls. Plant Physiology (1992), 100(4), 2041-5

Guo, Lining; Arteca, RN. Hormonal induction of 1-aminocyclopropane-1-carboxylic acid malonyltransferase in etiolated mung bean hypocotyl segments (1991). In “Biosynthesis and Molecular Regulation of Amino Acids”. H. Flores et al. Eds. American Society of Plant Physiologists, p298-301

Liu, Y; Hua, X; Guo Lining. Effect of 2,5-norbornadiene on the ripening and production of ethylene in tomato fruits. In “Biochemical and Physiological aspects of Ethylene Production in Lower and Higher Plants”. H. Clijsters et al. Eds. Kluwer Academic Publishers (1989), p101-108.

Issued Patents

United States Patent 6,331,664. Acyl-ACP thioesterase nucleic acids from maize and methods of altering palmitic acid levels in transgenic plants therewith

Inventors: Rubin-Wilson, Beth; Guo, Lining; Skokut, Tom; Young, Scott; Folkerts, Otto; Armstrong, Katherine; Cowen, Neil M.

United States Patent 6,350,934. Nucleic acid encoding delta-9 desaturase

Inventors: Zwick, Michael G.; Edington, Brent E.; McSwiggen, James A.; Merlo, Patricia Ann Owens; Guo, Lining; Skokut, Thomas A.; Young, Scott A.; Folkerts, Otto; Merlo, Donald J.

United States Patent 6,528,484. Insecticidal protein toxins from Photorhabdus

Inventors: Ensign, Jerald C.; Bowen, David J.; Petell, James; Fatig, Raymond; Schoonover, Sue; ffrench-Constant, Richard H.; Rocheleau, Thomas A.; Blackburn,Michael B.; Hey, Timothy D.; Merlo, Donald J.; Orr, Gregory L.; Roberts, Jean L.; Strickland, James A.; Guo, Lining; Ciche, Todd A.; Sukhapinda, Kitisri

United States Patent 6,590,142. Transgenic plants expressing photorhabdus toxin

Inventors: Petell; James K.; Merlo; Donald J.; Herman; Rod A.; Roberts; Jean L.; Guo; Lining; Schafer; Barry W.; Sukhapinda; Kitisri; Merlo; Ann Owens

United States Patent 6,717,035 Transgenic plants expressing photorhabdus toxin

Inventors: Petell; James K. ; Merlo; Donald J.; Herman; Rod A.; Roberts; Jean L.; Guo; Lining; Schafer; Barry W.; Sukhapinda; Kitisri; Merlo; Ann Owens

United States Patent 6,770,452. Methods for the identification of inhibitors of serine acetyltransferase activity in plantsInventors: Rice; John W; Guo; Lining; Davis; Keith; Zayed; Adel; Ascenzi; Robert; Mitchell; Joseph Cameron; Riggsbee; Daniel N; Boyes; Douglas; Mulpuri; Rao; Hoffman; Neil; Kjemtrup; Susanne; Hamilton; Carol; Woessner; Jeffrey; Gorlach; Jorn

United States Patent 7,071,386. Xenorhabdus TC gene for pest control

Inventors: Bintrim; Scott B., Mitchell; Jon C., Larrinua; Ignacio M., Apel-Birkhold; Patricia C., Green; Susan B., Schafer; Barry W., Bevan; Scott A., Young; Scott A., Guo; Lining