Supplementary Table 1 Distribution of specific activity (nmol min-1 mg-1 protein) of the different membrane marker enzymes in the microsomes after two-phase partition.
Marker enzymes / Microsomes / Upper phase / Lower phaseVO43-–ATPase (PM) / 103.6 / 367.0 / 28.6
NO3-–ATPase (TP) / 32.1 / 0 / 67.4
NaN3–ATPase (Mitochondria) / 11.5 / 0 / 24.7
Latent–UDPase (Golgi) / 21.4 / 4.1 / 37.9
NADPH–CytC reductase (ER) / 7.6 / 1.8 / 8.9
*PM, plasma membrane; TP, tonoplast; ER, endoplasmic reticulum.
**Activities of ATPase were assayed according to Serrano (1978, Mol Cell Biochem, vol22, p51-63), with the concentrations of inhibitors Na3VO4, KNO3 and NaN3 were 0.1 mM, 50 mM and1mMrespectively. Latent-UDPase activity was assayed according to Ray et al. (1969, PNAS, vol64, p605-612). NADPH-CytC reductase activity was assayed according to Leonard (1973, Plant Physiol, vol51, p749-754).
Supplementary Table 2Primers used for Real-Time PCR analysis.
MdCRY2
(Li et al. 2013b) / AAGATGTGGGAAATGGAAGCA / TAGAGGAGTATGTAGGGCAAGGAGA
AB710143 / MdHY5 / GTCTTCGAGCTCTGCATTCC / CCTCAACAACCTCTTCAGCC
AB668570 / MdCOP1 / AGGAGGACATTACTGCCGTTGA / AATGCCGTTGCCATGCTGAT
DQ267896 / MdMYB10 / TGCCTGGACTCGAGAGGAAGACA / CCTGTTTCCCAAAAGCCTGTGAA
JQ248934 / MdPAL / TTGACGCACAAGTTGAAGCA / CCACTGAGGTGATGTTCGGA
CN944824 / MdCHS / GGAGACAACTGGAGAAGGACTGGAA / CGACATTGATACTGGTGTCTTCA
CN491664 / MdF3H / TGGAAGCTTGTGAGGACTGGGGT / CTCCTCCGATGGCAAATCAAAGA
AF117268 / MdDFR / GATAGGGTTTGAGTTCAAGTA / TCTCCTCAGCAGCCTCAGTTTTCT
AF117269 / MdANS / CCAAGTGAAGCGGGTTGTGCT / CAAAGCAGGCGGACAGGAGTAGC
AF117267 / MdUFGT1 / CACTTTCTGGATCTCCGGACTCAA / CCGAAAATGACTCCTTCCGCTAAG
AB169975 / MdUFGT2 / CTGCCGCCATCAGACGCTTTGAT / CCACAAGAAGGGAGCCCCTGT
CN938023 / MdActin / TGACCGAATGAGCAAGGAAATTACT / TACTCAGCTTTGGCAATCCACATC
SupplementaryFig.1Spectrum of sunlight and the effect of 3-mm commercial glass (Na2O·CaO·6SiO2) on the spectrum. Spectra were measured with a Unispec SC spectrometer (PP Systems,Amesbury, MA,USA). Each curve was an average of 10 independent measurements.
SupplementaryFig.2Fruitsof bagged ‘Golden Delicious’ after exposure to sunlight for 7 days.
SupplementaryFig.3Coding sequence comparison of MdUFGT1, MdUFGT2 and MdpUFGluT21. MdUFGT1 and MdUFGT2 were isolated from apple genome ( MdUFGT1 shared 100% similarity with UFGT1 (AF117267.1); MdUFGT2 shared 99.6% similarity with MdpUFGluT21 (AB169975.1), and shared 89.5% similarity with MdUFGT1.
Supplementary Fig.4 Phenolic compounds concentration in ‘Golden Delicious’ apple fruit peels, after directly exposing bagged fruits to sunlight (CK) or with UV attenuation by 3-mm glass (+Glass). The asterisk indicates asignificant difference between “CK” and “+Glass” at P0.05,t-test.Each datapoint represents mean ± SE (n = 5).
Supplementary Fig. 5 Transcription levels of MdCRY2 in‘Golden Delicious’ apple fruit peels, after directly exposing bagged fruits to sunlight (CK) or with UV attenuation by 3-mm glass (+Glass). The asterisk indicates asignificant difference between “CK” and “+Glass” at P0.05,t-test.Each datapoint represents mean ± SE (n = 5).
Supplementary Fig. 6 Phenolic compounds concentration in ‘Golden Delicious’ apple fruit peels, after bagged fruits were treated with different concentrations of hydrogen peroxide and directly exposed to sunlight (CK) or with UV attenuation by 3-mm glass (Glass) for 0 or 3 days. Each datapoint represents mean ± SE (n = 5).
Supplementary Fig. 7 Phenolic compounds concentration in ‘Golden Delicious’ apple fruit peels, after bagged fruits were treated with different concentrations of diphenyleneiodonium chloride (DPI) and directly exposed to sunlight (CK) or with UV attenuation by 3-mm glass (Glass) for 0 or 5 days. Different letters above the bars indicate significant differences at P < 0.05, least significant difference (LSD).Each datapoint represents mean ± SE (n = 5).