Plasmid Mapping Practice Problems

1.

Restriction Enzyme(s) / Number of base pairs
Bam HI / 52.0
Hind III / 26.0 / 12.0 / 8.0 / 6.0
Hind III & Bam HI / 14.0 / 12.0 / 8.0 / 6.0

2.

Restriction Enzyme(s) / Number of base pairs
Eco RI / 20.0
Bam HI / 12.0 / 2.0 / 6.0
Eco RI & Bam HI / 8.0 / 4.0 / 2.0 / 6.0

3.

Restriction Enzyme(s) / Number of base pairs
Hpa I / 26.0
Hind III / 13.0 / 6.0 / 4.0 / 3.0
Hind III & Hpa I / 7.0 / 6.0 / 4.0 / 3.0

4.

Restriction Enzyme(s) / Number of base pairs
Hind III / 42.0 / 31.0 / 14.0
Bam HI / 46.0 / 36.0 / 5.0
Hind III & Bam HI / 24.0 / 22.0 / 15.0 / 14.0 / 7.0 / 5.0

5.

Restriction Enzyme(s) / Number of base pairs
Pst I / 4.36
EcoRI / 4.36
Sal I / 4.36
Eco RI & Sal I / 3.98 / 0.38
Eco RI & Pst I / 3.61 / 0.75 /
Pst I & SaI I / 3.23 / 1.13

6.

Restriction Enzyme(s) / Number of base pairs
Bam HI / 20.0 /
Hind III / 11.0 / 6.0 / 3.0
Hind III & Bam HI / 7.0 / 6.0 / 3.0