Plant Cell Reports

Plant Cell Reports

Supplementary Information

Supplemental Figures 1-5

Guihua Lu • Xiping Wang • Junhua Liu • Kun Yu •Yang Gao • Haiyan Liu • Changgui Wang • Wei Wang •

Guokui Wang • Min Liu • Guanfan Mao • Binfeng Li • Jianying Qin • Mian Xia • Junli Zhou • Jingmei Liu • Shuqin Jiang • Hua Mo • Jinteng Cui • Nobuhiro Nagasawa • Shoba Sivasankar • Marc C. Albertsen • Hajime Sakai • Barbara J. Mazur • Michael W. Lassner • Richard M. Broglie

Application of T-DNA activation tagging to identify glutamate receptor-like genes that enhance drought tolerance in plants

G. Lu (*) • X. Wang • J. Liu • Y. Gao • H. Liu • C. Wang • W. Wang • G. Wang • M. Liu • G. Mao • S. Jiang

Beijing Kaituo DNA Biotech Research Center, Co., Ltd., Beijing 102206, China

E-mail:

H. Mo• N. Nagasawa • M. C. Albertsen • H. Sakai • B. J. Mazur • M. W. Lassner • R. M. Broglie (*)

DuPont Pioneer Agriculture Biotechnology, Johnston, Iowa, IA 50131 or Wilmington, Delaware, DE 19880, USA

E-mail:

K. Yu (e-mail: )

B. Li (e-mail: )

J. Qin (e-mail: )

M. Xia (e-mail: )

J. Zhou (e-mail: )

J. Liu (e-mail: )

J. Cui (e-mail: )

N. Nagasawa (emmmail: )

S. Sivasankar (e-mail: )

Plant Cell Reports

1 AGATCTATATTTGTTTACACGGTCTTCAATGATATGCTTTGACCAATAATTTATTTCATG

61 TTATGTTCCTAACGAATATAAAATTAGTATCACATGAAAATACTTTAAAATATAAATTAA

121 GTGATATACTTCCTCCGTTTTACAATGTAAGTCATTTTAGCAACTTCTAACTGAAACTTG

181 AAAACCTTGGTAGACGGCATGTAACCAGCTTTATGCCGTCGACAACCATGGTCTAGACAG

241 GATCCCC

1 CCGCGCCGATGGTGCCGTTGAGCAGCGCCTCGTGGAAGCTCTGCGCCGCCCCGTACGGCA

61 CCAGCCTCGCCGGCGGGAACCCGGACCTGGTCATGGCCCCCCGCATGAAGGAGTTGTTCA

121 TGATCCCCACGCGCTCCGCGCCGCGCCACAGCGCCGCGTAGCCGGCGATGGACGGCTCGA

181 GGCGCGGCACGGTGAGCATGGAGGTGAGGCTGGCGGTGTAGCTGGACCGGAGGATGAGGA

241 CGACGAAGAACCAGACGACGGCGGCGAGGCGGGCGAGGTTGCTGCGGAGGTCCTCGCGGT

301 GGGCGAAGACGAGGGTGGAGAAGCCGAAGTAGAGGAGGGTGCCGAGCTGGTGCCACGGCG

361 GGCCGCGGAACTCGGCGTTGCCGCGGTGCTCGACGAACCAGACGGCGAAGCCGGTGAGGA

421 GGAGGAAGGCAGCGCTGGCGAGCGACAGGTCGTAGCGGAGCGGCTTCAGGAACACCCACG

481 TCCGCTCCCCGCCGCGGCCGACGTCCCGCAGCGGCGCCACCATGGCGATGCCCGACGCCA

541 TGAACGGCAGGGTGAAGTCCACGTAGCTCGACCGCGCCGCCGTGATCGTCATGTCCGCCA

601 CCGCCGCGTCGAATGCCTGCACACAAATTTAATTTAAACGCCATGGAAAGGAATCCCCCG

661 TAGCTGCCGGTGCCGTGAGAGGAGGAGGAGGCCGCCTGCCGTGCCGGCTCACGTCTGCCG

721 CTCCGCCACGCAATTTCTGGATGCCGACAGCGGAGCAAGTCCAACGGTGGAGCGGAACTC

781 TCGAGAGGGGTCCAGA

Supplemental Fig. 1 Nucleotide sequences (from 5’ to 3’ end) of the T-DNA right border (a) and

left border (b)-flanks in AH01486 genome. T-DNA sequences were underlined

Plant Cell Reports

1 ATGGCTGGTCACACCCCGAATCCTCTCTTCCTCCTCCTCTTCCTCGGATACCTCCTCTTC

61 GCGGCGGCGCAGCCGCCGCTGACGGTCACCGTCGGCCTCATCATCGACGGCGGCTCGCCG

121 GTCGGCAAGATCGCCAACACCACCATCCCCATGGCGCTCGACGACTTCTACGCCGCCTTC

181 CCGCGGTCCCCCGCCCGCGTCCGCCTCCTGCACCGCGACTCCCGCGGCGACGTCGTCGCC

241 GCCGCCTCCGCCGCGCTGGAGCTGATGGAGGGGCGGGGCGTGCGCGCCATCCTTGGGCCT

301 CAGTCCTCCGTGGAGTCGGCGTTCGTCGCCGACCTCGCCACGCGGGCGGAGGTCCCCGTC

361 GTGTCGTTCTCGGCGACCAGCCCGTCGGTGTCGCCCGGCGGAGGGAGGTTCTTCGCGCGC

421 GCCGCGCTGAGCGACGCGGCGCAGGCGGGCGCCATCGCCGCGCTCGCGAGGCTCTTCGGG

481 TGGCGCCGCGTCGTGCCGGTCTACCAGGACGACGACTACGGCGCCGCCTTCGTGCCGTTC

541 CTCGTCGACGCGCTCACCGCCGAGGGCTCCGAGGTCCCCTACCGCTGCGCGCTCCCGGCG

601 GGGGCCGACGCCGACGCCGTCGCCGCCGCGATGTACCGGATGGAGTCGCTGCAGACGCGC

661 GCCTTCGTGCTGCACGCGCGCCCGGACCTCGCCGGGCGCGTGCTCGCCGCCGCCGAGGCC

721 GCCGGGATGATGGGCGAGGGCTTCGCGTGGGTCATCACCGACGGGCTCACGGGCCTCCTC

781 GGCTCCATCAACGCGCCGCAGGGGGTGATCGGCCTCGCCCCCTACGTGCCGACGACGCCG

841 CGGCTGCGCGACGTCAGGCGGCGGTGGGTGCGCCGCTTCATGGCCGAGCACCCGGCCGCC

901 GACGCGGAGCACGCCGAGATGGGCAGCTACGCCGTGTGGGCCTACGACGCCGCGTGGGCC

961 GTCGCGTCCGCCGCCGAACACCTCACCGCCGGCGACCTATCACCGCCGCAGGGTGGGCTC

1021 GTGGGGGGCAAGGGCGGACCCACCGACTTCGCCGGGCTCGGCAAGTCAAGGTCCGGCAAG

1081 AAGTTCCTCGAGGCGATCACCAGCACGACGTTCGACGGCCTCGGCGGCAGGTTCCAGCTC

1141 GTCGACGGCGAGCTCGCGGTGCACGCCTTCCGCGTGCTGAACATCATGGACAGAGGGAAG

1201 GAGAGGAGCATCGGGTTCTGGACCAAGGACGGCGGGCTGACCCGGCACCTCGGCGTTGGC

1261 GGCGGTGGCGGGGGCGAGCTCGCGCCGGTGATCTGGCCGGGGGAGTCGACGGTGGTGCCG

1321 AGGGGGTGGGTGGTGCCGACGAGCGCGCGGAGGCTGCGGGTGGCGGTGCCCGGCAGCGTC

1381 AACCCCGGGTACCGGGCGATCGTGCACCTGGACGTGGACGCGGCGACGAACCGGACGACC

1441 GCCGGCGGGTTCGTCGTCGAGGTGTTCGAGGCGGCGGTGCGGCTGCTGCCGTACGCGCTG

1501 CCGGTGGAGTACGTGAAGGCGGAGTCGATGCCCTACGACAAGCTGGTGCAGATGGTTGCT

1561 GATGGGGCATTCGACGCGGCGGTGGCGGACATGACGATCACGGCGGCGCGGTCGAGCTAC

1621 GTGGACTTCACCCTGCCGTTCATGGCGTCGGGCATCGCCATGGTGGCGCCGCTGCGGGAC

1681 GTCGGCCGCGGCGGGGAGCGGACGTGGGTGTTCCTGAAGCCGCTCCGCTACGACCTGTGG

1741 CTCGCCAGCGCTGCCTTCCTCCTCCTCACCGGCTTCGCCGTCTGGTTCGTCGAGCACCGC

1801 GGCAACGCCGAGTTCCGCGGCCCGCCGTGGCACCAGCTCGGCACCCTCCTCTACTTCGGC

1861 TTCTCCACCCTCGTCTTCGCCCACCGCGAGGACCTCCGCAGCAACCTCGCCCGCCTCGCC

1921 GCCGTCGTCTGGTTCTTCGTCGTCCTCATCCTCCAGTCCAGCTACACCGCCAGCCTCACC

1981 TCCATGCTCACCGTGCCGCGCCTCGAGCCGTCCATCGCCGGCTACGCGGCGCTGTGGCGC

2041 GGCGCGGAGCGCGTGGGGATCATGAACAACTCCTTCATGCGGGGGGCCATGACCAGGTCC

2101 GGGTTCCCGCCGGCGAGGCTGGTGCCGTACGGGGCGGCGCAGAGCTTCCACGAGGCGCTG

2161 CTCAACGGCACCATCGGCGCGGTGGTGGACGAGACGCCGTACCTGAGGATCTTCCTCAAG

2221 TCCTACTGCGACAGGTTCGCCATGGCCGGCGGCGGCGGCGGGGGGCAGCCCAACAAGACC

2281 GGCGGCTTCGGCTTCGCCTTCCCCAAGGGCTCGCCGTACGTCGCCGACCTGTCCCGCGCC

2341 ATCCTGGCGCTGACGGAGAGCGAGGAGATGAACCTCATCGAGCGCAAGTGGTTCGGCGAG

2401 TCCGACGGCTGCGCGGCGGCGCAGGCGGCCGGCGGGCCGTTCACGTCGGACAGCCTCAGC

2461 TTCGGCAGCTTCTGGGGCCTCTTCCTCATCACCGGCGCCACCTCGCTCCTCTGCTGCGCC

2521 GTCCACCTCGCCACCTTCGTCGCCTCCAACCGGGGTGCCATCCGCGACATCGTCGCGACG

2581 TCGACGCACCCGTTCCGGAGGCTCGCCGAGCTCTACGACGGCAGGGACCTCTCGGCGCAC

2641 ACGTTCAAGGCCAAGGACGGCGGCGCCGCCGCCTCGCCGCCCGTCGTGCACGACGCGGCC

2701 GGCAGCCCGATCAGCCTGCACATGGGCGCCTGGTCGCCGCAGCCGTCGAGCACCATGGCC

2761 GGCGGCGAGATCGAGCCGGCCGGTGGCGAAGCCAACGAGGAGGAGGCGACGACGACGACT

2821 GCAGCTCGCGATCCCGACGGCGCTGGAGAGAATGGCAGAGGCCAGTAA

1 MAGHTPNPLFLLLFLGYLLFAAAQPPLTVTVGLIIDGGSPVGKIANTTIPMALDDFYAAF

61 PRSPARVRLLHRDSRGDVVAAASAALELMEGRGVRAILGPQSSVESAFVADLATRAEVPV

121 VSFSATSPSVSPGGGRFFARAALSDAAQAGAIAALARLFGWRRVVPVYQDDDYGAAFVPF

181 LVDALTAEGSEVPYRCALPAGADADAVAAAMYRMESLQTRAFVLHARPDLAGRVLAAAEA

241 AGMMGEGFAWVITDGLTGLLGSINAPQGVIGLAPYVPTTPRLRDVRRRWVRRFMAEHPAA

301 DAEHAEMGSYAVWAYDAAWAVASAAEHLTAGDLSPPQGGLVGGKGGPTDFAGLGKSRSGK

361 KFLEAITSTTFDGLGGRFQLVDGELAVHAFRVLNIMDRGKERSIGFWTKDGGLTRHLGVG

421 GGGGGELAPVIWPGESTVVPRGWVVPTSARRLRVAVPGSVNPGYRAIVHLDVDAATNRTT

481 AGGFVVEVFEAAVRLLPYALPVEYVKAESMPYDKLVQMVADGAFDAAVADMTITAARSSY

541 VDFTLPFMASGIAMVAPLRDVGRGGERTWVFLKPLRYDLWLASAAFLLLTGFAVWFVEHR

601 GNAEFRGPPWHQLGTLLYFGFSTLVFAHREDLRSNLARLAAVVWFFVVLILQSSYTASLT

661 SMLTVPRLEPSIAGYAALWRGAERVGIMNNSFMRGAMTRSGFPPARLVPYGAAQSFHEAL

721 LNGTIGAVVDETPYLRIFLKSYCDRFAMAGGGGGGQPNKTGGFGFAFPKGSPYVADLSRA

781 ILALTESEEMNLIERKWFGESDGCAAAQAAGGPFTSDSLSFGSFWGLFLITGATSLLCCA

841 VHLATFVASNRGAIRDIVATSTHPFRRLAELYDGRDLSAHTFKAKDGGAAASPPVVHDAA

901 GSPISLHMGAWSPQPSSTMAGGEIEPAGGEANEEEATTTTAARDPDGAGENGRGQ

Supplemental Fig. 2 Nucleotide sequences of OsGLR1 open reading frame (a, from 5’ to 3’ end) and its corresponding amino acid sequences (b, from N-terminus to C-terminus)

Plant Cell Reports

1 ATGGAAGCTGGAGCAAGATTAGCCTTCGTAATGCCGCTCGTCGTCGTCCTCTTGCTGATG

61 ATCTTCTCGTTGGGCGTTCGGGGAGTGGATGTGGTCGTCGATGGAGGAGGAGGAGGTGCG

121 GCGGCTGCTCGGCGGAGGAGGGTGGAGGTGGGGGTGATCCTGGACAGGAGGACGTGGCTG

181 GGGAACATCAGCTGGGCGTGCATGGAGCTCGCCGTGGAGGATTTCTACGCCGACGAGGAG

241 CGCGCCAGCTACACCACCGCGCTCAGGCTCCACCTCCGGGACACCCGCCTCGACGCCGTC

301 GACGCCGCCTCCGCAGGTGTCGATCTACTGAAAAATGTCCATGTGCAAGCAATCGTTGGG

361 CCACAGACATCAGCACAGGCCAAATTTCTCGCGGAGCTTGGGGAAAAATCATCGGTTCCT

421 GTTGTTTCATTCTCTGCAAATAGCCCATGTCGAACGGCCAGCCAAACTCCATACTTCATC

481 AGGACGGCATGGAACGACTCCTCACAAGCAGAAGCTATCGCGTCACTTGTTCAAAGATTC

541 AATTGGAGGGACGTCATCCCTGTCATCGAAGATGATGACTCCAATACAAGATTCATTCCT

601 GACCTTGTTGATGCCCTAAGGAATGCCGAGATCCGTGTTACCCACAGGTGCAAGATACAC

661 CCGTCAGCTGGAGCTGATGACATAAAGAAAGTCGTCTTAAGCTTAAAGGAAAAATGGACA

721 AGCGTATTTGTCGTGCGCATGTCGTATCAGCTAGCTCTTAGTTTTTTCAAGCATGCCAAG

781 GATGAAGGGATGATGGGCCAGGGCTTTGTCTGGATTGCAGCTTATGGCTTAACAGACATC

841 TTTGATGTAGTTGGTTCTCCTGCATTTGATGTGATGCAAGGAGTTATTGGGATGAAACCT

901 TATGTTAATGATACTAAACAACTTCAAAACTTCAGGCAGAGATGGCGCAAGATGTATAAG

961 TCTGAAAATCCAGGCACCACATTGAGTGAGCCCACATTATCTGGTCTCTACGCATATGAC

1021 ACTGTGTGGGCATTAGCGTTAGCAGCAGAGAAGGCTGGATATGTTAACTCAGACTTTCTT

1081 CTGTCTGAAAAAAACAACGGGTCGACTGATTTTGACAGGATCAATACTTCAAATGCTGCT

1141 AAGAAGCTCCAGAGCACACTCTTGAATATTGATTTCCAAGGCATGAGTGGGAAATTTCAG

1201 TTTCAAGACATGCATTTGCTATCGATGACTTATGAGATAATAAATATAGTTGGGGAGGAG

1261 CAAAGAGTAGTTGGTTTTTGGACACCAGAATTTAACATTTCCAGGGGTCTAAATACGAAA

1321 GCTGATGTTAATGAAATCATATGGCCAGGAGGAGAAACAACTGTACCAAGGGGGTGGCTC

1381 TTTCCAATGAATAAAACACTCAAAATAGGCGTGCCTGCAAAACCTGGGTTTTCTGGTTTT

1441 ATAAAGAAAGAAAAATATAATTTCACAGGACTCTGCATTGAGGTATTCGAGGAAGTTCTT

1501 AATGGATTACCCTACAAAATACCCCATGACTATGTGGAGTTTGGAAATGGAAAGGGGGAA

1561 AGTAATGGAACTTACGATGAACTTATCTACAAAGTTTATCAGAACGACTTCGATGCAGCA

1621 GTTGGTGATATAACAATCTTGGCCAACCGATCTTTATATGTGGACTTCACTCTTCCTTAT

1681 ACTGAGTCAGGAGTGCGCATGCTAGTTCCAGTCCAGGACCAGCGACAGAAGACTGCATGG

1741 ACGTTTCTGCAGCCATTAACAGCTGACTTATGGTTAGGAACTGCGGCCTTCTTTGTCTTA

1801 ACAGGTTTTGTAGTTTGGTTTATTGAGCACAGAACAAATGAAGATTTCAGAGGACCACCA

1861 GTCAATCAAATTGGATCTGTCTTCTACTTCGCCTTCTCCACACTTGTTTTTGCTCATCGG

1921 CAAAAGATTGTGAACAATTTATCAAGAGTTTTGTTAGTTATTTGGCTTTTCGTGGTGCTG

1981 ATATTGCAGCGGAGTTATACTGCAAGCTTGAGCTCAATTCTCACAGTGGAACAACTTCAG

2041 CCAACAGTCACCAATTTAGACGAAGTCATCAGGAAAGGGGCCAACGTTGGCTACCTCAAT

2101 GATTCTTTCATGCCTGAGCTTTTGAAAAGGTTGAAAATTGACGAATCAAAGTTGATTGCC

2161 CTTGACTCTCCTGATGAATACAACGAGGCTCTATCAACTGGTAGAGTTGCTGTAGTTGTC

2221 GATGAGATACCATACCTTAAGGTGTTTCTTTCAAAGTATTGCCACAACTATACTATGGTT

2281 GGCCCAACTTACAAGTTCGATGGATTTGGTTTTGCATTCCCTCTAGGCTCCCCGCTCACT

2341 GCTGAGATTTCAAGGGGAATATTGAACTTCACATCAAGCAACAGAATGGCCCAACTGGAG

2401 AGAGAATTGTACAACAATAGAACATGCCCTGACAAAGATGACTCCCAAACTTCAAGCAGC

2461 CTTACACTACGCAGCTTTCTTGGGCTGTTCATCATCACTGGAGCATCTTCACTGCTGGCA

2521 CTGTTCCTACATGTTGTCATAACCCTCTATAATCATCGGCATGATTTGAGCAGCGCCAGC

2581 AGTAGTCAGAGTTCATGGTGTGGATGGTTTGCCATCCTCCTAAAGATATTCCACGAGGGT

2641 GATAGACCTAATGCTCCACAATTGGATGAGCCAGCAGTGTCAAATGCTAACACTACAGCT

2701 GATACTCCATGGAGCACACCTGATCATCACATTATCGAAAATGTTGATTCAGGCAGTGAT

2761 GTGGAGAGCGTAAGAGAAGAAGATAGAGAAGATTTTGTTCAGGGCCCTGACCCACCATCT

2821 TTTGCATACATGCATTCAGAGAGGGGGCAATAA

1 MEAGARLAFVMPLVVVLLLMIFSLGVRGVDVVVDGGGGGAAAARRRRVEVGVILDRRTWL

61 GNISWACMELAVEDFYADEERASYTTALRLHLRDTRLDAVDAASAGVDLLKNVHVQAIVG

121 PQTSAQAKFLAELGEKSSVPVVSFSANSPCRTASQTPYFIRTAWNDSSQAEAIASLVQRF

181 NWRDVIPVIEDDDSNTRFIPDLVDALRNAEIRVTHRCKIHPSAGADDIKKVVLSLKEKWT

241 SVFVVRMSYQLALSFFKHAKDEGMMGQGFVWIAAYGLTDIFDVVGSPAFDVMQGVIGMKP

301 YVNDTKQLQNFRQRWRKMYKSENPGTTLSEPTLSGLYAYDTVWALALAAEKAGYVNSDFL

361 LSEKNNGSTDFDRINTSNAAKKLQSTLLNIDFQGMSGKFQFQDMHLLSMTYEIINIVGEE

421 QRVVGFWTPEFNISRGLNTKADVNEIIWPGGETTVPRGWLFPMNKTLKIGVPAKPGFSGF

481 IKKEKYNFTGLCIEVFEEVLNGLPYKIPHDYVEFGNGKGESNGTYDELIYKVYQNDFDAA

541 VGDITILANRSLYVDFTLPYTESGVRMLVPVQDQRQKTAWTFLQPLTADLWLGTAAFFVL

601 TGFVVWFIEHRTNEDFRGPPVNQIGSVFYFAFSTLVFAHRQKIVNNLSRVLLVIWLFVVL

661 ILQRSYTASLSSILTVEQLQPTVTNLDEVIRKGANVGYLNDSFMPELLKRLKIDESKLIA

721 LDSPDEYNEALSTGRVAVVVDEIPYLKVFLSKYCHNYTMVGPTYKFDGFGFAFPLGSPLT

781 AEISRGILNFTSSNRMAQLERELYNNRTCPDKDDSQTSSSLTLRSFLGLFIITGASSLLA

841 LFLHVVITLYNHRHDLSSASSSQSSWCGWFAILLKIFHEGDRPNAPQLDEPAVSNANTTA

901 DTPWSTPDHHIIENVDSGSDVESVREEDREDFVQGPDPPSFAYMHSERGQ

Supplemental Fig. 3 Nucleotide sequences of OsGLR2 open reading frame (a, from 5’ to 3’ end) and its corresponding amino acid sequences (b, from N-terminus to C-terminus)

Plant Cell Reports

1 ATGGCTGGTCACACCCCGAATCCTCTCTTCCTCCTCCTCTTCCTCGGATACCTCCTCTTC

61 GCGGCGGCGCAGCCGCCGCTGACGGTCACCGTCGGCCTCATCATCGACGGCGGCTCGCCG

121 GTCGGCAAGATCGCCAACACCACCATCCCCATGGCGCTCGACGACTTCTACGCCGCCTTC

181 CCGCGGTCCCCCGCCCGCGTCCGCCTCCTGCACCGCGACTCCCGCGGCGACGTCGTCGCC

241 GCCGCCTCCGCCGGTACGGACACGCTCGTTTCGTCTCGTTTCGTAGCTTGAGCTTGTGAT

301 GATGAATTAATGGACGTCGATCGTCGCGTGCTGCAGCGCTGGAGCTGATGGAGGGGCGGG

361 GCGTGCGCGCCATCCTTGGGCCTCAGTCCTCCGTGGAGTCGGCGTTCGTCGCCGACCTCG

421 CCACGCGGGCGGAGGTCCCCGTCGTGTCGTTCTCGGCGACCAGCCCGTCGGTGTCGCCCG

481 GCGGAGGGAGGTTCTTCGCGCGCGCCGCGCTGAGCGACGCGGCGCAGGCGGGCGCCATCG

541 CCGCGCTCGCGAGGCTCTTCGGGTGGCGCCGCGTCGTGCCGGTCTACCAGGACGACGACT

601 ACGGCGCCGCCTTCGTGCCGTTCCTCGTCGACGCGCTCACCGCCGAGGGCTCCGAGGTCC

661 CCTACCGCTGCGCGCTCCCGGCGGGGGCCGACGCCGACGCCGTCGCCGCCGCGATGTACC

721 GGATGGAGTCGCTGCAGACGCGCGCCTTCGTGCTGCACGCGCGCCCGGACCTCGCCGGGC

781 GCGTGCTCGCCGCCGCCGAGGCCGCCGGGATGATGGGCGAGGGCTTCGCGTGGGTCATCA

841 CCGACGGGCTCACGGGCCTCCTCGGCTCCATCAACGCGCCGCAGGGGGTGATCGGCCTCG

901 CCCCCTACGTGCCGACGACGCCGCGGCTGCGCGACGTCAGGCGGCGGTGGGTGCGCCGCT

961 TCATGGCCGAGCACCCGGCCGCCGACGCGGAGCACGCCGAGATGGGCAGCTACGCCGTGT

1021 GGGCCTACGACGCCGCGTGGGCCGTCGCGTCCGCCGCCGAACACCTCACCGCCGGCGACC

1081 TATCACCGCCGCAGGGTGGGCTCGTGGGGGGCAAGGGCGGACCCACCGACTTCGCCGGGC

1141 TCGGCAAGTCAAGGTCCGGCAAGAAGTTCCTCGAGGCGATCACCAGCACGACGTTCGACG

1201 GCCTCGGCGGCAGGTTCCAGCTCGTCGACGGCGAGCTCGCGGTGCACGCCTTCCGCGTGC

1261 TGAACATCATGGACAGAGGGAAGGAGAGGAGCATCGGGTTCTGGACCAAGGACGGCGGGC

1321 TGACCCGGCACCTCGGCGTTGGCGGCGGTGGCGGGGGCGAGCTCGCGCCGGTGATCTGGC

1381 CGGGGGAGTCGACGGTGGTGCCGAGGGGGTGGGTGGTGCCGACGAGCGCGCGGAGGCTGC

1441 GGGTGGCGGTGCCCGGCAGCGTCAACCCCGGGTACCGGGCGATCGTGCACCTGGACGTGG

1501 ACGCGGCGACGAACCGGACGACCGCCGGCGGGTTCGTCGTCGAGGTGTTCGAGGCGGCGG

1561 TGCGGCTGCTGCCGTACGCGCTGCCGGTGGAGTACGTGAAGGCGGAGTCGATGCCCTACG

1621 ACAAGCTGGTGCAGATGGTTGCTGATGGGGTAAGTGCTTGTGCTTCTAGTACATCAAATA

1681 TACTCCCTCCATCTCAAAATGTTTAA