Pi-h1_t_-1 cDNA HR4 ATCGAGGCGCGAGTAGGGATGGAGGCGGCTGCCATGGCCGTAACCGCAGCCACGGGGGCC 60
Pikp-1 cds K60 ------ATGGAGGCGGCTGCCATGGCCGTAACCGCAGCCACGGGGGCC 42
Pikh-1 cds K3 ------ATGGAGGCGGCTGCCATGGCCGTAACCGCAGCCACGGGGGCC 42
Pik1 cds Kanto51 ------ATGGAGGCGGCTGCCATGGCCGTAACCGCAGCCACGGGGGCC 42
Pikm1 cds Tsuyuake ------ATGGAGGCGGCTGCCATGGCCGTAACCGCAGCCACGGGGGCC 42
******************************************
Pi-h1_t_-1 cDNA HR4 TTGGCGCCCGTGCTAGTGAAGCTGGCCGCTTTGCTGGACGACGGGGAGTGCAATCTTCTG 120
Pikp-1 cds K60 TTGGCGCCCGTGCTAGTGAAGCTGGCCGCTTTGCTGGACGACGGGGAGTGCAATCTTCTG 102
Pikh-1 cds K3 TTGGCGCCCGTGCTAGTGAAGCTGGCCGCTTTGCTGGACGACGGGGAGTGCAATCTTCTG 102
Pik1 cds Kanto51 TTGGCGCCCGTGCTAGTGAAGCTGGCCGCTTTGCTGGACGACGGGGAGTGCAATCTTCTG 102
Pikm1 cds Tsuyuake TTGGCGCCCGTGCTAGTGAAGCTGGCCGCTTTGCTGGACGACGGGGAGTGCAATCTTCTG 102
************************************************************
Pi-h1_t_-1 cDNA HR4 GAGGGGAGCCGGAGCGACGCAGAGTTCATCAGATCCGAGCTGGAGGCCGTTCATTCTCTC 180
Pikp-1 cds K60 GAGGGGAGCCGGAGCGACGCAGAGTTCATCAGATCCGAGCTGGAGGCCGTTCATTCTCTC 162
Pikh-1 cds K3 GAGGGGAGCCGGAGCGACGCAGAGTTCATCAGATCCGAGCTGGAGGCCGTTCATTCTCTC 162
Pik1 cds Kanto51 GAGGGGAGCCGGAGCGACGCAGAGTTCATCAGATCCGAGCTGGAGGCCGTGCACTCTCTC 162
Pikm1 cds Tsuyuake GAGGGGAGCCGGAGCGACGCAGAGTTCATCAGATCCGAGCTGGAGGCCGTGCACTCTCTC 162
************************************************** ** ******
Pi-h1_t_-1 cDNA HR4 CTCACCCCAAATATCTTGGGGAGGATGGGGGATGACGATGCGGCGTGCAAGGATGGCTTG 240
Pikp-1 cds K60 CTCACCCCAAATATCTTGGGGAGGATGGGGGATGACGATGCGGCGTGCAAGGATGGCTTG 222
Pikh-1 cds K3 CTCACCCCAAATATCTTGGGGAGGATGGGGGATGACGATGCGGCGTGCAAGGATGGCTTG 222
Pik1 cds Kanto51 CTCACCCCAAATATCTTGGGGAGGATGGGGGATGACGATGCGGCGTGCAAGGATGGCTTG 222
Pikm1 cds Tsuyuake CTCACCCCAAATATCTTGGGGAGGATGGGGGATGACGATGCGGCGTGCAAGGATGGCTTG 222
************************************************************
Pi-h1_t_-1 cDNA HR4 ATTGCGGAGGTCCGGGAGCTGTCCTACGACCTGGATGATGCCGTCGACGACTTCTTGGAG 300
Pikp-1 cds K60 ATTGCGGAGGTCCGGGAGCTGTCCTACGACCTGGATGATGCCGTCGACGACTTCTTGGAG 282
Pikh-1 cds K3 ATTGCGGAGGTCCGGGAGCTGTCCTACGACCTGGATGATGCCGTCGACGACTTCTTGGAG 282
Pik1 cds Kanto51 ATTGCGGAGGTCCGGGAGCTGTCCTACGACCTGGATGATGCCGTCGACGACTTCTTGGAG 282
Pikm1 cds Tsuyuake ATTGCGGAGGTCCGGGAGCTGTCCTACGACCTGGATGATGCCGTCGACGACTTCTTGGAG 282
************************************************************
Pi-h1_t_-1 cDNA HR4 CTCAATTTCGAGCAGCGAAGAAGCGCAAGCCCTTTCGGTGAGCTCAAGGCAAGAGTTGAG 360
Pikp-1 cds K60 CTCAATTTCGAGCAGCGAAGAAGCGCAAGCCCTTTCGGTGAGCTCAAGGCAAGAGTTGAG 342
Pikh-1 cds K3 CTCAATTTCGAGCAGCGAAGAAGCGCAAGCCCTTTCGGTGAGCTCAAGGCAAGAGTTGAG 342
Pik1 cds Kanto51 CTCAATTTCGAGCAGCGAAGAAGCGCAAGCCCTTTCGGTGAGCTCAAGGCAAGAGTTGAG 342
Pikm1 cds Tsuyuake CTCAATTTCGAGCAGCGAAGAAGCGCAAGCCCTTTCGGTGAGCTCAAGGCAAGAGTTGAG 342
************************************************************
Pi-h1_t_-1 cDNA HR4 GAGCATGTCTCCAATCGCTTCTCTGACTGGAAGCTACCGGCGGCGAGCCTTCCGCCGTCG 420
Pikp-1 cds K60 GAGCATGTCTCCAATCGCTTCTCTGACTGGAAGCTACCGGCGGCGAGCCTTCCGCCGTCG 402
Pikh-1 cds K3 GAGCATGTCTCCAATCGCTTCTCTGACTGGAAGCTACCGGCGGCGAGCCTTCCGCCGTCG 402
Pik1 cds Kanto51 GAGCGTGTCTCCAATCGCTTCTCTGACTGGAAGCTACCGGCGGCGAGCCTTCCGCCGTCG 402
Pikm1 cds Tsuyuake GAGCGTGTCTCCAATCGCTTCTCTGACTGGAAGCTACCGGCGGCGAGCCTTCCGCCGTCG 402
**** *******************************************************
Pi-h1_t_-1 cDNA HR4 TCGGTACACCGCCGAGCTGGCTTGCCGCCACCAGATGCAGAGCTGGTGGGGATGGACAAA 480
Pikp-1 cds K60 TCGGTACACCGCCGAGCTGGCTTGCCGCCACCAGATGCAGAGCTGGTGGGGATGGACAAA 462
Pikh-1 cds K3 TCGGTACACCGCCGAGCTGGCTTGCCGCCACCAGATGCAGAGCTGGTGGGGATGGACAAA 462
Pik1 cds Kanto51 TCGGTACACCGTCGAGCTGGCTTGCCGCCACCAGATGCAGGGCTGGTGGGGATGCACAAA 462
Pikm1 cds Tsuyuake TCGGTACACCGTCGAGCTGGCTTGCCGCCACCAGATGCAGGGCTGGTGGGGATGCACAAA 462
*********** **************************** ************* *****
Pi-h1_t_-1 cDNA HR4 CGTATGGAAGAGCTCACCAAATTGCTGGAACAAGGGAGCAATGATGCTTCACGATGGCGC 540
Pikp-1 cds K60 CGTATGGAAGAGCTCACCAAATTGCTGGAACAAGGGAGCAATGATGCTTCACGATGGCGC 522
Pikh-1 cds K3 CGTATGGAAGAGCTCACCAAATTGCTGGAACAAGGGAGCAATGATGCTTCACGATGGCGC 522
Pik1 cds Kanto51 CGTAAGGAAGAGCTCATCGAGTTGCTGGAACAAGGGAGCAGTGATGCTTCACGATGGCGC 522
Pikm1 cds Tsuyuake CGTAAGGAAGAGCTCATCGAGTTGCTGGAACAAGGGAGCAGTGATGCTTCACGATGGCGC 522
**** *********** * * ******************* *******************
Pi-h1_t_-1 cDNA HR4 AAGCGAAAACCGCATTTCCCGCTCAGAAAAAC---AGGGCTAAAGCAAAAAATCGTGATC 597
Pikp-1 cds K60 AAGCGAAAACCGCATTTCCCGCTCAGAAAAAC---AGGGCTAAAGCAAAAAATCGTGATC 579
Pikh-1 cds K3 AAGCGAAAACCGCATTTCCCGCTCAGAAAAAC---AGGGCTAAAGCAAAAAATCGTGATC 579
Pik1 cds Kanto51 AAGCGAAAACCTCATGTCCCCCTCAGGATAATGGGAGGGGAAATGCAAAAAATCGTGTTC 582
Pikm1 cds Tsuyuake AAGCGAAAACCTCATGTCCCCCTCAGGATAATGGGAGGGGAAATGCAAAAAATCGTGTTC 582
*********** *** **** ***** * ** **** ** ************* **
Pi-h1_t_-1 cDNA HR4 AAGGTTGCCATGGAGGGCAATAATTGCCGTTCAAAAGCAATGGCTTTAGTTGCGAGCACT 657
Pikp-1 cds K60 AAGGTTGCCATGGAGGGCAATAATTGCCGTTCAAAAGCAATGGCTTTAGTTGCGAGCACT 639
Pikh-1 cds K3 AAGGTTGCCATGGAGGGCAATAATTGCCGTTCAAAAGCAATGGCTTTAGTTGCGAGCACT 639
Pik1 cds Kanto51 AAGATTCCCATGGTGGACGATAAGAGCCGTACAAAAGCAATGTCATTGGTTGCAAGTACG 642
Pikm1 cds Tsuyuake AAGATTCCCATGGTGGACGATAAGAGCCGTACAAAAGCAATGTCATTGGTTGCAAGTACG 642
*** ** ****** ** * **** ***** *********** * ** ***** ** **
Pi-h1_t_-1 cDNA HR4 GGAGGAGTGGACTCGGTTGCGCTCGTAGGTGATCTAAGAGACAAGATAGAGGTGGTCGGT 717
Pikp-1 cds K60 GGAGGAGTGGACTCGGTTGCGCTCGTAGGTGATCTAAGAGACAAGATAGAGGTGGTCGGT 699
Pikh-1 cds K3 GGAGGAGTGGACTCGGTTGCGCTCGTAGGTGATCTAAGAGACAAGATAGAGGTGGTCGGT 699
Pik1 cds Kanto51 GTTGGAGTGCACTCGGTTGCAATCGCCGGTGACCTAAGAGACGAGGTTGTGGTGGTCGGT 702
Pikm1 cds Tsuyuake GTTGGAGTGCACTCGGTTGCAATCGCCGGTGACCTAAGAGACCAGGTTGTGGTGGTCGGT 702
* ****** ********** *** ***** ********* ** * * **********
Pi-h1_t_-1 cDNA HR4 TATGGCATTGACCCCATCAAGCTGATCTCCGCGCTCCGGAAGAAGGTGGGCGATGCGGAG 777
Pikp-1 cds K60 TATGGCATTGACCCCATCAAGCTGATCTCCGCGCTCCGGAAGAAGGTGGGCGATGCGGAG 759
Pikh-1 cds K3 TATGGCATTGACCCCATCAAGCTGATCTCCGCGCTCCGGAAGAAGGTGGGCGATGCGGAG 759
Pik1 cds Kanto51 GATGGCATTGACTCCATCAATCTGGTCTCTGCGCTCCGGAAGAAGGTGGGCCATGCGGAG 762
Pikm1 cds Tsuyuake GATGGCATTGACTCCATCAATCTGGTCTCTGCGCTCCGGAAGAAGGTGGGCCCTGCGATG 762
*********** ******* *** **** ********************* **** *
Pi-h1_t_-1 cDNA HR4 TTGCTGCAGGTCAGCCAAGCAAAAAAAGATGTGAAGGAGACGACGCCGATGCTTGCGCCG 837
Pikp-1 cds K60 TTGCTGCAGGTCAGCCAAGCAAATAAAGATGTGAAGGAGACGACGCCGATGCTTGCGCCG 819
Pikh-1 cds K3 TTGCTGCAGGTCAGCCAAGCAAAGAAAGATGTGAAGGAGACGACGCCGATGCTTGCGCCG 819
Pik1 cds Kanto51 TTGCTGCAGGTCAGCCAAGTAAAGGAAGATGTGAAGGAGATAACGGCGATGCTTGCGCCG 822
Pikm1 cds Tsuyuake TTTCTGGAGGTCAGCCAAGTAAAGGAAGATGTGAAGGAGATAACGGCGATGCTTGCGCCG 822
** *** ************ *** *************** *** **************
Pi-h1_t_-1 cDNA HR4 GTGAAATCCATATGTGAATTTCACAAGGTCAAAACAGTTTGCATCCTTGGATTGCCAGGT 897
Pikp-1 cds K60 GTGAAATCCATATGTGAATTTCACAAGGTCAAAACAGTTTGCATCCTTGGATTGCCAGGT 879
Pikh-1 cds K3 GTGAAATCCATATGTGAATTTCACAAGGTCAAAACAGTTTGCATCCTTGGATTGCCAGGT 879
Pik1 cds Kanto51 GTGAAATCCATATGTGAATTTCACGAGGTCAAAACAATTTGCATCCTTGGATTGCCAGGG 882
Pikm1 cds Tsuyuake GTGAAATCCATATGTGAATTTCACGAGGTCAAAACAATTTGCATCCTTGGATTGCCAGGG 882
************************ *********** **********************
Pi-h1_t_-1 cDNA HR4 GGAGGCAAAACAACGGTTGCCAGAGAATTATATGACGCCTTGGGAACGCACTTCCCATGC 957
Pikp-1 cds K60 GGAGGCAAAACAACGGTTGCCAGAGAATTATATGACGCCTTGGGAACGCACTTCCCATGC 939
Pikh-1 cds K3 GGAGGCAAAACAACGGTTGCCAGAGAATTATATGACGCCTTGGGAACGCACTTCCCATGC 939
Pik1 cds Kanto51 GGAGGCAAAACAACGATTGCCCGAGTACTATATCATGCATTGGGAACGCAGTTCCAATGC 942
Pikm1 cds Tsuyuake GGAGGCAAAACAACGATTGCCCGAGTACTATATCATGCATTGGGAACGCAGTTCCAATGC 942
*************** ***** *** * ***** * ** *********** **** ****
Pi-h1_t_-1 cDNA HR4 CGGGTTTTCGTGTCAGTCTCTCCAAGTTCTAGTCCCAGTCCCAATCTCACAAAGACTCTT 1017
Pikp-1 cds K60 CGGGTTTTCGTGTCAGTCTCTCCAAGTTCTAGTCCCAGTCCCAATCTCACAAAGACTCTT 999
Pikh-1 cds K3 CGGGTTTTCGTGTCAGTCTCTCCAAGTTCTAGTCCCAGTCCCAATCTCACAAAGACTCTT 999
Pik1 cds Kanto51 CGGGTTTTCGCATCAATCTCTCCAAGTTCCAGCCCCAGTCCCAATCTAACAGAGACTCTT 1002
Pikm1 cds Tsuyuake CGGGTTTTCGCATCAATCTCTCCAAGTTCCAGCCCCAGTCCCAATCTAACAGAGACTCTT 1002
********** *** ************* ** ************** *** ********
Pi-h1_t_-1 cDNA HR4 GCAGACATTTTCGCTCAAGCACAACTAGGAGTAACCGATACACTTAGCACACCATATGGT 1077
Pikp-1 cds K60 GCAGACATTTTCGCTCAAGCACAACTAGGAGTAACCGATACACTTAGCACATCATATGGT 1059
Pikh-1 cds K3 GCAGACATTTTCGCTCAAGCACAACTAGGAGTAACTGATACACTTAGCACACCATATGGT 1059
Pik1 cds Kanto51 GCAGACATTTTCGCTCAAGCACAACTAGGAGTAACTGATACACTTAGCACACCATATGGT 1062
Pikm1 cds Tsuyuake GCAGACATTTTCGCTCAAGCACAACTAGGAGTAACTGATACACTTAGCACACCATATGGT 1062
*********************************** *************** ********
Pi-h1_t_-1 cDNA HR4 GGGAGTGGGACCGGGAGAGCTCTTCAACAACATCTCATCGACAACATATCAGCTTTCCTC 1137
Pikp-1 cds K60 GGGAGTGGGACCGGGAGAGCTCTTCAACAACATCTCATCGACAACATATCAGCTTTCCTC 1119
Pikh-1 cds K3 GGGAGTGGGACCGGGAGAGCTCTTCAACAACATCTCATCGACAACATATCAGCTTTCCTC 1119
Pik1 cds Kanto51 GGGAGTGGGACCGGGAGAGCTCTTCAACAACATCTCATCGACAACATATCAGCTTTCCTC 1122
Pikm1 cds Tsuyuake GGGAGTGGGACCGGGAGAGCTCTTCAACAACATCTCATCGACAACATATCAGCTTTCCTC 1122
************************************************************
Pi-h1_t_-1 cDNA HR4 CTCAACAAAAAGTATCTCATTGTAATCGATGACATTTGGCATTGGGAAGAATGGGAAGTC 1197
Pikp-1 cds K60 CTCAACAAAAAGTATCTCATTGTAATCGATGACATTTGGCATTGGGAAGAATGGGAAGTC 1179
Pikh-1 cds K3 CTCAACAAAAAGTATCTCATTGTAATCGATGACATTTGGCATTGGGAAGAATGGGAAGTC 1179
Pik1 cds Kanto51 CTCAACAAAAAGTATCTCATTGTAATCGATGACATTTGGCATTGGGAAGAATGGGAAGTC 1182
Pikm1 cds Tsuyuake CTCAACAAAAAGTATCTCATTGTAATCGATGACATTTGGCATTGGGAAGAATGGGAAGTC 1182
************************************************************
Pi-h1_t_-1 cDNA HR4 ATCAGAAAGTCCATTCCCAAGAATGATCTGGGTGGTAGAATAATCATGACTACTCGTCTT 1257
Pikp-1 cds K60 ATCAGAAAGTCCATTCCCAAGAATGATCTGGGTGGTAGAATAATCATGACTACTCGTCTT 1239
Pikh-1 cds K3 ATCAGAAAGTCCATTCCCAAGAATGATCTGGGTGGTAGAATAATCATGACTACTCGTCTT 1239
Pik1 cds Kanto51 ATCAGAAAGTCCATTCCCAAGAATGATCTGGGTGGTAGAATAATCATGACTACTCGTCTT 1242
Pikm1 cds Tsuyuake ATCAGAAAGTCCATTCCCAAGAATGATCTGGGTGGTAGAATAATCATGACTACTCGTCTT 1242
************************************************************
Pi-h1_t_-1 cDNA HR4 AATTCAATAGCTGAGAAGTGCCACACTGATGACAATGATGTTTTTGTCTACGAAGTTGGG 1317
Pikp-1 cds K60 AATTCAATAGCTGAGAAGTGCCACACTGATGACAATGATGTTTTTGTCTACGAAGTTGGG 1299
Pikh-1 cds K3 AATTCAATAGCTGAGAAGTGCCACACTGATGACAATGATGTTTTTGTCTACGAAGTTGGG 1299
Pik1 cds Kanto51 AATTCAATAGCTGAGAAGTGCCACACTGATGACAATGATGTTTTTGTCTACGAAGTTGGG 1302
Pikm1 cds Tsuyuake AATTCAATAGCTGAGAAGTGCCACACTGATGACAATGATGTTTTTGTCTACGAAGTTGGG 1302
************************************************************
Pi-h1_t_-1 cDNA HR4 GATCTAGATAATAATGATGCTTTGTCGTTGTCTTGGGGGATAGCAACAAAGTCTGGGGCA 1377
Pikp-1 cds K60 GATCTAGATAATAATGATGCTTTGTCGTTGTCTTGGGGGATAGCAACAAAGTCTGGGGCA 1359
Pikh-1 cds K3 GATCTAGATAATAATGATGCTTTGTCGTTGTCTTGGGGGATAGCAACAAAGTCTGGGGCA 1359
Pik1 cds Kanto51 GATCTAGATAATAATGATGCTTTGTCGTTGTCTTGGGGGATAGCAACAAAGTCTGGGGCA 1362
Pikm1 cds Tsuyuake GATCTAGATAATAATGATGCTTGGTCGTTGTCTTGGGGGATAGCAACAAAGTCTGGGGCA 1362
********************** *************************************
Pi-h1_t_-1 cDNA HR4 GGCAACAGGATCGGAACTGGAGAGGATAATCCATGCTATGATATTGTGAACATGTGTTAT 1437
Pikp-1 cds K60 GGCAACAGGATCGGAACTGGAGAGGATAATCCATGCTATGATATTGTGAACATGTGTTAT 1419
Pikh-1 cds K3 GGCAACAGGATCGGAACTGGAGAGGATAATCCATGCTATGATATTGTGAACATGTGTTAT 1419
Pik1 cds Kanto51 GGCAACAGGATCGGAACTGGAGAGGATAATCCATGCTATGATATTGTGAACATGTGTTAT 1422
Pikm1 cds Tsuyuake GGCAACAGGATCGGAACTGGAGAGGATAATTCATGCTATGATATTGTGAACATGTGTTAT 1422
****************************** *****************************
Pi-h1_t_-1 cDNA HR4 GGTATGCCTTTAGCACTTATTTGGCTGTCGTCAGCATTGGTTGGAGAGATAGAAGAATTA 1497
Pikp-1 cds K60 GGTATGCCTTTAGCACTTATTTGGCTGTCGTCAGCATTGGTTGGAGAGATAGAAGAATTA 1479
Pikh-1 cds K3 GGTATGCCTTTAGCACTTATTTGGCTGTCGTCAGCATTGGTTGGAGAGATAGAAGAATTA 1479
Pik1 cds Kanto51 GGTATGCCTTTAGCACTTATTTGGCTGTCGTCAGCATTGGTTGGAGAGATAGAAGAATTA 1482
Pikm1 cds Tsuyuake GGTATGCCTTTAGCACTTATTTGGCTGTCGTCAGCATTGGTTGGAGAGATAGAAGAATTA 1482
************************************************************
Pi-h1_t_-1 cDNA HR4 GGTGGTGCTGAAGTGAAAAAATGTAGGGATTTGAGACACATAGAGGATGGTATTTTGGAC 1557
Pikp-1 cds K60 GGTGGTGCTGAAGTGAAAAAATGTAGGGATTTGAGACACATAGAGGATGGTATTTTGGAC 1539
Pikh-1 cds K3 GGTGGTGCTGAAGTGAAAAAATGTAGGGATTTGAGACACATAGAGGATGGTATTTTGGAC 1539
Pik1 cds Kanto51 CGTGGTGCTGAAGTGAAAAAATGTAGGGATTTGAGACACATAGAGGATGGTATTTTGGAC 1542
Pikm1 cds Tsuyuake GGTGGTGCTGAAGTGAAAAAATGTAGGGATTTGAGACACATAGAGGATGGTATTTTGGAC 1542
***********************************************************
Pi-h1_t_-1 cDNA HR4 ATCCCATCCTTACAACCATTGGCGGAGAGTTTATGCCTTGGTTATAACCATCTTCCTCTT 1617
Pikp-1 cds K60 ATCCCATCCTTACAACCATTGGCGGAGAGTTTATGCCTTGGTTATAACCATCTTCCTCTT 1599
Pikh-1 cds K3 ATCCCATCCTTACAACCATTGGCGGAGAGTTTATGCCTTGGTTATAACCATCTTCCTCTT 1599
Pik1 cds Kanto51 ATCCCATCCTTACAACCATTGGCGGAGAGTTTATGCCTTGGTTATAACCATCTTCCTCTT 1602
Pikm1 cds Tsuyuake ATCCCATCCTTACAACCATTGGCGGAGAGTTTATGCCTTGGTTATAACCATCTTCCTCTT 1602
************************************************************
Pi-h1_t_-1 cDNA HR4 TATCTGAGGACTTTGTTGTTGTACTGTAGTGCATACCATTGGTCTAACAGAATCGAAAGG 1677
Pikp-1 cds K60 TATCTGAGGACTTTGTTGTTGTACTGTAGTGCATACCATTGGTCTAACAGAATCGAAAGG 1659
Pikh-1 cds K3 TATCTGAGGACTTTGTTGTTGTACTGTAGTGCATACCATTGGTCTAACAGAATCGAAAGG 1659
Pik1 cds Kanto51 TATCTGAGGACTTTGTTGTTGTACTGTAGTGCATACCATTGGTCTAACAGAATCGAAAGG 1662
Pikm1 cds Tsuyuake TATCTGAGGACTTTGTTGTTGTACTGTAGTGCATACCATTGGTCTAACAGAATCGAAAGG 1662
************************************************************
Pi-h1_t_-1 cDNA HR4 GGTCGTCTGGTCAGGAGGTGGATTGCGGAAGGATTTGTGTCGGAAGAGAAAGAAGCAGAA 1737
Pikp-1 cds K60 GGTCGTCTGGTCAGGAGGTGGATTGCGGAAGGATTTGTGTCGGAAGAGAAAGAAGCAGAA 1719
Pikh-1 cds K3 GGTCGTCTGGTCAGGAGGTGGATTGCGGAAGGATTTGTGTCGGAAGAGAAAGAAGCAGAA 1719
Pik1 cds Kanto51 GGTCGTCTGGTCAGGAGGTGGATTGCGGAAGGATTTGTGTCGGAAGAGAAAGAAGCAGAA 1722
Pikm1 cds Tsuyuake GGTCGTCTGGTCAGGAGGTGGATTGCGGAAGGATTTGTGTCGGAAGAGAAAGAAGCAGAA 1722
************************************************************
Pi-h1_t_-1 cDNA HR4 GGTTACTTTGGCGAGCTTATTAACAGAGGATGGATTACGCAGCACGGAGACAACAACAGT 1797
Pikp-1 cds K60 GGTTACTTTGGCGAGCTTATTAACAGAGGATGGATTACGCAGCACGGAGACAACAACAGT 1779
Pikh-1 cds K3 GGTTACTTTGGCGAGCTTATTAACAGAGGATGGATTACGCAGCACGGAGACAACAACAGT 1779
Pik1 cds Kanto51 GGTTACTTTGGCGAGCTTATTAACAGAGGATGGATTACGCAGCACGGAGACAACAACAGT 1782
Pikm1 cds Tsuyuake GGTTACTTTGGCGAGCTTATTAACAGAGGATGGATTACGCAGCACGGAGACAACAACAGT 1782
************************************************************
Pi-h1_t_-1 cDNA HR4 TATAATTACTATGAGATCCACCCCGTGATGCTGGCCTTCCTGAGATGCAAGTCCAAGGAG 1857
Pikp-1 cds K60 TATAATTACTATGAGATCCACCCCGTGATGCTGGCCTTCCTGAGATGCAAGTCCAAGGAG 1839
Pikh-1 cds K3 TATAATTACTATGAGATCCACCCCGTGATGCTGGCCTTCCTGAGATGCAAGTCCAAGGAG 1839
Pik1 cds Kanto51 TATAATTACTATGAGATCCACCCCGTGATGCTGGCCTTCCTGAGATGCAAGTCCAAGGAG 1842
Pikm1 cds Tsuyuake TATAATTACTATGAGATCCACCCCGTGATGCTGGCCTTCCTGAGATGCAAGTCCAAGGAG 1842
************************************************************
Pi-h1_t_-1 cDNA HR4 TACAATTTTTTAACATGCTTGGGTCTGGGATCTGATACTAGTACTAGTGCATCCTCCCCA 1917
Pikp-1 cds K60 TACAATTTTTTAACATGCTTGGGTCTGGGATCTGATACTAGTACTAGTGCATCCTCCCCA 1899
Pikh-1 cds K3 TACAATTTTTTAACATGCTTGGGTCTGGGATCTGATACTAGTACTAGTGCATCCTCCCCA 1899
Pik1 cds Kanto51 TACAATTTTTTAACATGCTTGGGTCTGGGATCTGATACTAGTACTAGTGCATCCTCCCCA 1902
Pikm1 cds Tsuyuake TACAATTTTTTAACATGCTTGGGTCTGGGATCTGATACTAGTACTAGTGCATCCTCCCCA 1902
************************************************************
Pi-h1_t_-1 cDNA HR4 AGGTTGATTCGCCGGCTGTCTCTTCAGGGGGGGTATCCAGTGGACTGCTTGTCAAGCATG 1977
Pikp-1 cds K60 AGGTTGATTCGCCGGCTGTCTCTTCAGGGGGGGTATCCAGTGGACTGCTTGTCAAGCATG 1959
Pikh-1 cds K3 AGGTTGATTCGCCGGCTGTCTCTTCAGGGGGGGTATCCAGTGGACTGCTTGTCAAGCATG 1959
Pik1 cds Kanto51 AGGTTGATTCGCCGGCTGTCTCTTCAGGGGGGGTATCCAGTGGACTGCTTGTCAAGCATG 1962
Pikm1 cds Tsuyuake AGGTTGATTCGCCGGCTGTCTCTTCAGGGGGGGTATCCAGTGGACTGCTTGTCAAGCATG 1962
************************************************************
Pi-h1_t_-1 cDNA HR4 AGTATGGATGTGTCACACACTTGCAGCCTTGTCGTCCTTGGTGACGTGGCGCGACCCAAG 2037
Pikp-1 cds K60 AGTATGGATGTGTCACACACTTGCAGCCTTGTCGTCCTTGGTGACGTGGCGCGACCCAAG 2019
Pikh-1 cds K3 AGTATGGATGTGTCACACACTTGCAGCCTTGTCGTCCTTGGTGACGTGGCGCGACCCAAG 2019
Pik1 cds Kanto51 AGTATGGATGTGTCACACACTTGCAGCCTTGTCGTCCTTGGTGACGTGGCGCGACCCAAG 2022
Pikm1 cds Tsuyuake AGTATGGATGTGTCACACACTTGCAGCCTTGTCGTCCTTGGTGACGTGGCGCGACCCAAG 2022
************************************************************
Pi-h1_t_-1 cDNA HR4 GGAATCCCCTTCTATATGTTTAAGCGCTTGCGAGTGTTGGACCTTGAAGATAATAAGGAT 2097
Pikp-1 cds K60 GGAATCCCCTTCTATATGTTTAAGCGCTTGCGAGTGTTGGACCTTGAAGATAATAAGGAT 2079
Pikh-1 cds K3 GGAATCCCCTTCTATATGTTTAAGCGCTTGCGAGTGTTGGACCTTGAAGATAATAAGGAT 2079
Pik1 cds Kanto51 GGAATCCCCTTCTATATGTTTAAGCGCTTGCGAGTGTTGGACCTTGAAGATAATAAGGAT 2082
Pikm1 cds Tsuyuake GGAATCCCCTTCTATATGTTTAAGCGCTTGCGAGTGTTGGACCTTGAAGATAATAAGGAT 2082
************************************************************
Pi-h1_t_-1 cDNA HR4 ATACAGGATTCTCATCTGCAGGGCATATGTGAACAGTTAAGCCTCAGAGTGAGGTACCTT 2157
Pikp-1 cds K60 ATACAGGATTCTCATCTGCAGGGCATATGTGAACAGTTAAGCCTCAGAGTGAGGTACCTT 2139
Pikh-1 cds K3 ATACAGGATTCTCATCTGCAGGGCATATGTGAACAGTTAAGCCTCAGAGTGAGGTACCTT 2139
Pik1 cds Kanto51 ATACAGGATTCTCATCTGCAGGGCATATGTGAACAGTTAAGCCTCAGAGTGAGGTACCTT 2142
Pikm1 cds Tsuyuake ATACAGGATTCTCATCTGCAGGGCATATGTGAACAGTTAAGCCTCAGAGTGAGGTACCTT 2142
************************************************************
Pi-h1_t_-1 cDNA HR4 GGTCTCAAGGGAACGCGGATCCGAAAGCTCCCTCAGGAGATGAGGAAGCTGAAGCATTTG 2217
Pikp-1 cds K60 GGTCTCAAGGGAACGCGGATCCGAAAGCTCCCTCAGGAGATGAGGAAGCTGAAGCATTTG 2199
Pikh-1 cds K3 GGTCTCAAGGGAACGCGGATCCGAAAGCTCCCTCAGGAGATGAGGAAGCTGAAGCATTTG 2199
Pik1 cds Kanto51 GGTCTCAAGGGAACGCGGATCCGAAAGCTCCCTCAGGAGATGAGGAAGCTGAAGCATTTG 2202
Pikm1 cds Tsuyuake GGTCTCAAGGGAACGCGGATCCGAAAGCTCCCTCAGGAGATGAGGAAGCTGAAGCATTTG 2202
************************************************************
Pi-h1_t_-1 cDNA HR4 GAGATTTTGTATGTGGGGAGCACTCGGATCAGTGAACTTCCGCAAGAGATTGGAGAGCTG 2277
Pikp-1 cds K60 GAGATTTTGTATGTGGGGAGCACTCGGATCAGTGAACTTCCGCAAGAGATTGGAGAGCTG 2259
Pikh-1 cds K3 GAGATTTTGTATGTGGGGAGCACTCGGATCAGTGAACTTCCGCAAGAGATTGGAGAGCTG 2259
Pik1 cds Kanto51 GAGATTTTGTATGTGGGGAGCACTCGGATCAGTGAACTTCCGCAAGAGATTGGAGAGCTG 2262
Pikm1 cds Tsuyuake GAGATTTTGTATGTGGGGAGCACTCGGATCAGTGAACTTCCGCAAGAGATTGGAGAGCTG 2262
************************************************************
Pi-h1_t_-1 cDNA HR4 AAGCATCTGCGGATTCTGGACGTGAGAAACACGGACATCACTGAGCTCCCACTGCAGATA 2337
Pikp-1 cds K60 AAGCATCTGCGGATTCTGGACGTGAGAAACACGGACATCACTGAGCTCCCACTGCAGATA 2319
Pikh-1 cds K3 AAGCATCTGCGGATTCTGGACGTGAGAAACACGGACATCACTGAGCTCCCACTGCAGATA 2319
Pik1 cds Kanto51 AAGCATCTGCGGATTCTGGACGTGAGAAACACGGACATCACTGAGCTCCCACTGCAGATA 2322
Pikm1 cds Tsuyuake AAGCATCTGCGGATTCTGGACGTGAGAAACACGGACATCACTGAGCTCCCACTGCAGATA 2322
************************************************************
Pi-h1_t_-1 cDNA HR4 CGGGAGCTGCAGCATCTGCACACTCTGGACGTGAGGAACACTCCAATCAGTGAGCTCCCG 2397
Pikp-1 cds K60 CGGGAGCTGCAGCATCTGCACACTCTGGACGTGAGGAACACTCCAATCAGTGAGCTCCCG 2379
Pikh-1 cds K3 CGGGAGCTGCAGCATCTGCACACTCTGGACGTGAGGAACACTCCAATCAGTGAGCTCCCG 2379
Pik1 cds Kanto51 CGGGAGCTGCAGCATCTGCACACTCTGGACGTGAGGAACACGCCAATCAGTGAGCTCCCG 2382
Pikm1 cds Tsuyuake CGGGAGCTGCAGCATCTGCACACTCTGGACGTGAGGAACACTCCAATCAGTGAGCTCCCG 2382
***************************************** ******************
Pi-h1_t_-1 cDNA HR4 CCGCAGGTTGGCAAGCTGCAGAATCTCAAGATTATGTGCGTGAGGAGCACTGGGGTTAGG 2457
Pikp-1 cds K60 CCGCAGGTTGGCAAGCTGCAGAATCTCAAGATTATGTGCGTGAGGAGCACTGGGGTTAGG 2439
Pikh-1 cds K3 CCGCAGGTTGGCAAGCTGCAGAATCTCAAGATTATGTGCGTGAGGAGCACTGGGGTTAGG 2439
Pik1 cds Kanto51 CCGCAGGTTGGCAAGCTGCAGAATCTCAAGATTATGTGCGTGAGGAGCACTGGGGTTAGG 2442
Pikm1 cds Tsuyuake CCGCAGGTTGGCAAGCTGCAGAATCTCAAGATTATGTGCGTGAGGAGCACTGGGGTTAGG 2442
************************************************************
Pi-h1_t_-1 cDNA HR4 GAGCTCCCAAAGGAGATTGGGGAGCTGAATCATCTACAGACTCTGGACGTGAGAAACACG 2517
Pikp-1 cds K60 GAGCTCCCAAAGGAGATTGGGGAGCTGAATCATCTACAGACTCTGGACGTGAGAAACACG 2499
Pikh-1 cds K3 GAGCTCCCAAAGGAGATTGGGGAGCTGAATCATCTACAGACTCTGGACGTGAGAAACACG 2499
Pik1 cds Kanto51 GAGCTCCCAAAGGAGATTGGGGAGCTGAATCATCTACAGACTCTGGACGTGAGAAACACG 2502
Pikm1 cds Tsuyuake GAGCTCCCAAAGGAGATTGGGGAGCTGAATCATCTACAGACTCTGGACGTGAGAAACACG 2502
************************************************************
Pi-h1_t_-1 cDNA HR4 AGGGTGAGAGAGCTGCCATGGCAAGCTGGCCAGATCTCCCAATCGTTGCGCGTGCTTGCC 2577
Pikp-1 cds K60 AGGGTGAGAGAGCTGCCATGGCAAGCTGGCCAGATCTCCCAATCGTTGCGCGTGCTTGCC 2559
Pikh-1 cds K3 AGGGTGAGAGAGCTGCCATGGCAAGCTGGCCAGATCTCCCAATCGTTGCGCGTGCTTGCC 2559
Pik1 cds Kanto51 AGGGTGAGAGAGCTGCCATGGCAAGCTGGCCAGATCTCCCAATCGTTGCGCGTGCTTGCC 2562
Pikm1 cds Tsuyuake AGGGTGAGAGAGCTGCCATGGCAAGCTGGCCAGATCTCCCAATCGTTGCGCGTGCTTGCC 2562
************************************************************
Pi-h1_t_-1 cDNA HR4 GGTGACAGTGGCGATGGCGTGCGGTTGCCCGAAGGCGTCTGCGAAGCTCTGATCAACGGT 2637
Pikp-1 cds K60 GGTGACAGTGGCGATGGCGTGCGGTTGCCCGAAGGCGTCTGCGAAGCTCTGATCAACGGT 2619
Pikh-1 cds K3 GGTGACAGTGGCGATGGCGTGCGGTTGCCCGAAGGCGTCTGCGAAGCTCTGATCAACGGT 2619
Pik1 cds Kanto51 GGTGACAGTGGCGATGGCGTGCGGTTGCCCGAAGGCGTCTGCGAAGCTCTGATCAACGGT 2622
Pikm1 cds Tsuyuake GGTGACAGTGGCGATGGCGTGCGGTTGCCCGAAGGCGTCTGCGAAGCTCTGATCAACGGT 2622
************************************************************
Pi-h1_t_-1 cDNA HR4 ATTCCAGGGGCTACGCGTGCAAAATGCAGGGAGGTTCTGTCCATCGCGATCATCGATCGT 2697
Pikp-1 cds K60 ATTCCAGGGGCTACGCGTGCAAAATGCAGGGAGGTTCTGTCCATCGCGATCATCGATCGT 2679
Pikh-1 cds K3 ATTCCAGGGGCTACGCGTGCAAAATGCAGGGAGGTTCTGTCCATCGCGATCATCGATCGT 2679
Pik1 cds Kanto51 ATTCCAGGGGCTACGCGTGCAAAATGCAGGGAGGTTCTGTCCATCGCGATCATCGATCGT 2682
Pikm1 cds Tsuyuake ATTCCAGGGGCTACGCGTGCAAAATGCAGGGAGGTTCTGTCCATCGCGATCATCGATCGT 2682
************************************************************
Pi-h1_t_-1 cDNA HR4 TTCGGACCTCCCCTTGTTGGTATATTCAAAGTTCCCGGCAGTCATATGCGTATCCCGAAG 2757
Pikp-1 cds K60 TTCGGACCTCCCCTTGTTGGGATATTCAAAGTTCCCGGCAGTCATATGCGTATCCCGAAG 2739
Pikh-1 cds K3 TTCGGACCTCCCCTTGTTGGGATATTCAAAGTTCCCGGCAGTCATATGCGTATCCCGAAG 2739
Pik1 cds Kanto51 TTCGGACCTCCCCTTGTTGGGATATTCAAAGTTCCCGGCAGTCATATGCGTATCCCGAAG 2742
Pikm1 cds Tsuyuake TTCGGACCTCCCCTTGTTGGGATATTCAAAGTTCCCGGCAGTCATATGCGTATCCCGAAG 2742
******************** ***************************************
Pi-h1_t_-1 cDNA HR4 ATGATCAAAGACCACTTCCGCGTTCTTTCTTGCCTAGACATCAGGCTCTGCCACAAGCTT 2817
Pikp-1 cds K60 ATGATCAAAGACCACTTCCGCGTTCTTTCTTGCCTAGACATCAGGCTCTGCCACAAGCTT 2799
Pikh-1 cds K3 ATGATCAAAGACCACTTCCGCGTTCTTTCTTGCCTAGACATCAGGCTCTGCCACAAGCTT 2799
Pik1 cds Kanto51 ATGATCAAAGACCACTTCCGCGTTCTTTCTTGCCTAGACATCAGGCTCTGCCACAAGCTT 2802
Pikm1 cds Tsuyuake ATGATCAAAGACCACTTCCGCGTTCTTTCTTGCCTAGACATCAGGCTCTGCCACAAGCTT 2802
************************************************************
Pi-h1_t_-1 cDNA HR4 GAGGATGATGACCAAAAGTTCCTCGCCGAGATGCCCAACCTGCAGACGCTCGTGCTGAGG 2877
Pikp-1 cds K60 GAGGATGATGACCAAAAGTTCCTCGCCGAGATGCCCAACCTGCAGACGCTCGTGCTGAGG 2859
Pikh-1 cds K3 GAGGATGATGACCAAAAGTTCCTCGCCGAGATGCCCAACCTGCAGACGCTCGTGCTGAGG 2859
Pik1 cds Kanto51 GAGGATGATGACCAAAAGTTCCTCGCCGAGATGCCCAACCTGCAGACGCTCGTGCTGAGG 2862
Pikm1 cds Tsuyuake GAGGATGATGACCAAAAGTTCCTCGCCGAGATGCCCAACCTGCAGACGCTCGTGCTGAGG 2862
************************************************************
Pi-h1_t_-1 cDNA HR4 TTCGAGGCCCTACCAAGACAACCCATAACCATCAACGGCACAGGCTTCCAGATGCTGGAG 2937
Pikp-1 cds K60 TTCGAGGCCCTACCAAGACAACCCATAACCATCAACGGCACAGGCTTCCAGATGCTGGAG 2919
Pikh-1 cds K3 TTCGAGGCCCTACCAAGACAACCCATAACCATCAACGGCACAGGCTTCCAGATGCTGGAG 2919
Pik1 cds Kanto51 TTCGAGGCCCTACCAAGACAACCCATAACCATCAACGGCACAGGCTTCCAGATGCTGGAG 2922
Pikm1 cds Tsuyuake TTCGAGGCCCTACCAAGACAACCCATAACCATCAACGGCACAGGCTTCCAGATGCTGGAG 2922
************************************************************
Pi-h1_t_-1 cDNA HR4 AGCTTCCGTGTCGACAGCCGGGTGCCAAGGATAGCCTTCCATGAAGACGCCATGCCCAAC 2997
Pikp-1 cds K60 AGCTTCCGTGTCGACAGCCGGGTGCCAAGGATAGCCTTCCATGAAGACGCCATGCCCAAC 2979
Pikh-1 cds K3 AGCTTCCGTGTCGACAGCCGGGTGCCAAGGATAGCCTTCCATGAAGACGCCATGCCCAAC 2979
Pik1 cds Kanto51 AGCTTCCGTGTCGACAGCCGGTTGCCAAGGATAGCCTTCCATGAAGACGCCATGCCCAAC 2982
Pikm1 cds Tsuyuake AGCTTCCGTGTCGACAGCCGGGTGCCAAGGATAGCCTTCCATGAAGACGCCATGCCCAAC 2982
********************* **************************************
Pi-h1_t_-1 cDNA HR4 CTCAAGCTTCTCGAGTTCAAGTTCTACGCCGGCCCAGCAAGCAACGATGCCATCGGCATC 3057
Pikp-1 cds K60 CTCAAGCTTCTCGAGTTCAAGTTCTACGCCGGCCCAGCAAGCAACGATGCCATCGGCATC 3039
Pikh-1 cds K3 CTCAAGCTTCTCGAGTTCAAGTTCTACGCCGGCCCAGCAAGCAACGATGCCATCGGCATC 3039
Pik1 cds Kanto51 CTCAAGCTTCTCGAGTTCAAGTTCTACGCCGGCCCAGCAAGCAACGATGCCATCGGCATC 3042
Pikm1 cds Tsuyuake CTCAAGCTTCTCGAGTTCAAGTTCTACGCCGGCCCAGCAAGCAACGATGCCATCGGCATC 3042
************************************************************
Pi-h1_t_-1 cDNA HR4 ACCAACCTGAAGAGCCTCCAAAAGGTGGTCTTTCGGTGCTCGCCATGGTACAAGAGCGAC 3117
Pikp-1 cds K60 ACCAACCTGAAGAGCCTCCAAAAGGTGGTCTTTCGGTGCTCGCCATGGTACAAGAGCGAC 3099
Pikh-1 cds K3 ACCAACCTGAAGAGCCTCCAAAAGGTGGTCTTTCGGTGCTCGCCATGGTACAAGAGCGAC 3099
Pik1 cds Kanto51 ACCAACCTGAAGAGCCTCCAAAAGGTGGTCTTTCGGTGCTCGCCATGGTACAAGAGCGAC 3102
Pikm1 cds Tsuyuake ACCAACCTGAAGAGCCTCCAAAAGGTGGTCTTTCGGTGCTCGCCATGGTACAAGAGCGAC 3102
************************************************************
Pi-h1_t_-1 cDNA HR4 GCCCCTGGCATCAGCGCCACCATTGACGTCGTGAAGAAAGAAGCCGAGGAGCATCCCAAC 3177
Pikp-1 cds K60 GCCCCTGGCATCAGCGCCACCATTGACGTCGTGAAGAAAGAAGCCGAGGAGCATCCCAAC 3159
Pikh-1 cds K3 GCCCCTGGCATCAGCGCCACCATTGACGTCGTGAAGAAAGAAGCCGAGGAGCATCCCAAC 3159
Pik1 cds Kanto51 GCCCCTGGCATCAGCGCCACCATTGACGTCGTGAAGAAAGAAGCCGAGGAGCATCCCAAC 3162
Pikm1 cds Tsuyuake GCCCCTGGCATCAGCGCCACCATTGACGTCGTGAAGAAAGAAGCCGAGGAGCATCCCAAC 3162
************************************************************
Pi-h1_t_-1 cDNA HR4 CGGCCGATCACCCTCCTCATCAATGCTGGGTATAAGGAGATATCAACTGAGTCACACGGG 3237
Pikp-1 cds K60 CGGCCGATCACCCTCCTCATCAATGCTGGGTATAAGGAGATATCAACTGAGTCACACGGG 3219
Pikh-1 cds K3 CGGCCGATCACCCTCCTCATCAATGCTGGGTATAAGGAGATATCAACTGAGTCACACGGG 3219
Pik1 cds Kanto51 CGGCCGATCACCCTCCTCATCAATGCTGGGTATAAGGAGATATCAACTGAGTCACACGGG 3222
Pikm1 cds Tsuyuake CGGCCGATCACCCTCCTCATCAATGCTGGGTATAAGGAGATATCAACTGAGTCACACGGG 3222
************************************************************
Pi-h1_t_-1 cDNA HR4 AGCAGTGAAAACATTGCGGGCAGCAGTGGGATCGATACTGAGCCTGCACAGGCACAGCAT 3297
Pikp-1 cds K60 AGCAGTGAAAACATTGCGGGCAGCAGTGGGATCGATACTGAGCCTGCACAGGCACAGCAT 3279
Pikh-1 cds K3 AGCAGTGAAAACATTGCGGGCAGCAGTGGGATCGATACTGAGCCTGCACAGGCACAGCAT 3279
Pik1 cds Kanto51 AGCAGTGAAAACATTGCGGGCAGCAGTGGGATCGATACTGAGCCTGCACAGGCACAGCAT 3282
Pikm1 cds Tsuyuake AGCAGTGAAAACATTGCGGGCAGCAGTGGGATCGATACTGAGCCTGCACAGGCACAGCAT 3282
************************************************************
Pi-h1_t_-1 cDNA HR4 GATAATCTCCCTGCTGTTCGAGATGACTACAAGGGAAAAGGGATTCTTCTTGATGGCAGG 3357
Pikp-1 cds K60 GATAATCTCCCTGCTGTTCGAGATGACTACAAGGGAAAAGGGATTCTTCTTGATGGCAGG 3339
Pikh-1 cds K3 GATAATCTCCCTGCTGTTCGAGATGACTACAAGGGAAAAGGGATTCTTCTTGATGGCAGG 3339
Pik1 cds Kanto51 GATAATCTCCCTGCTGTTCGAGATGACTACAAGGGAAAAGGGATTCTTCTTGATGGCAGG 3342
Pikm1 cds Tsuyuake GATAATCTCCCTGCTGTTCGAGATGACTACAAGGGAAAAGGGATTCTTCTTGATGGCAGG 3342
************************************************************
Pi-h1_t_-1 cDNA HR4 TGTCCTACCTGCGGCCGAGCGACTAAAATTGAAGAGGAAACCCAAGATCGAGTAGCAGAT 3417
Pikp-1 cds K60 TGTCCTACCTGCGGCCGAGCGACTAAAATTGAAGAGGAAACCCAAGATCGAGTAGCAGAT 3399
Pikh-1 cds K3 TGTCCTACCTGCGGCCGAGCGACTAAAATTGAAGAGGAAACCCAAGATCGAGTAGCAGAT 3399
Pik1 cds Kanto51 TGTCCTACCTGCGGCCGAGCGACTAAAATTGAAGAGGAAACCCAAGATCGAGTAGCAGAT 3402
Pikm1 cds Tsuyuake TGTCCTACCTGCGGCCGAGCGACTAAAATTGAAGAGGAAACCCAAGATCGAGTAGCAGAT 3402
************************************************************
Pi-h1_t_-1 cDNA HR4 ATTGAAATTCAAACAGAAACTACTAGCTAGCTAGTACCGCTGTCTAAT 3465
Pikp-1 cds K60 ATTGAAATTCAAACAGAAACTACTAGCTAG------3429
Pikh-1 cds K3 ATTGAAATTCAAACAGAAACTACTAGCTAG------3429
Pik1 cds Kanto51 ATTGAAATTCAAACAGAAACTACTAGCTAG------3432
Pikm1 cds Tsuyuake ATTGAAATTCAAACAGAAACTACTAGCTAG------3432
******************************
Supplementary Fig. A Alignment results of the cDNA sequence of Pi-h1(t)-1 from HR4 between the coding sequences (cds) of Pikp-1 from K60, Pikh-1 from K3, Pik1 from Kanto51 and Pikm1 from Tsuyuake using ClustalW2 multiple sequence alignment tool ( Specific markers amplifying the cDNA sequence from the RNAs of HR4 was developed according to the DNA sequence in shadow background. The coding sequences (cds) of Pikp-1 from K60, Pikh-1 from K3, Pik1 from Kanto51 and Pikm1 from Tsuyuake were obtained from NCBI. The two specific nucleotide bases in the cDNA sequence of Pi-h1(t)-1 from HR4 different from that of Pikp-1 from K60, Pikh-1 from K3, Pik1 from Kanto51 and Pikm1 from Tsuyuake were showed with red colour.
Pi-h1_t_-2 cDNA HR4 CAGCTGAAAGTTGCAGTGAGAAGTACAGAGAACAAGATGGAGTTGGTGGTAGGTGCTTCC 60
Pikp-2 cds K60 ------ATGGAGTTGGTGGTAGGTGCTTCC 24
Pikh-2 cds K3 ------ATGGAGTTGGTGGTAGGTGCTTCC 24
Pik2 cds Kanto51 ------ATGGAGTTGGTGGTAGGTGCTTCC 24
Pikm2 cds Tsuyuake ------ATGGAGTTGGTGGTAGGTGCTTCC 24
************************
Pi-h1_t_-2 cDNA HR4 GAAGCCACCATGAAATCTCTCTTGGGCAAGCTGGGCAATCTTCTAGCCCAGGAGTATGCT 120
Pikp-2 cds K60 GAAGCCACCATGAAATCTCTCTTGGGCAAGCTGGGCAATCTTCTAGCCCAGGAGTATGCT 84
Pikh-2 cds K3 GAAGCCACCATGAAATCTCTCTTGGGCAAGCTGGGCAATCTTCTAGCCCAGGAGTATGCT 84
Pik2 cds Kanto51 GAAGCCACCATGAAATCTCTCTTGGGCAAGCTGGGCAATCTTCTAGCCCAGGAGTATGCT 84
Pikm2 cds Tsuyuake GAAGCCACCATGAAATCTCTCTTGGGCAAGCTGGGCAATCTTCTAGCCCAGGAGTATGCT 84
************************************************************
Pi-h1_t_-2 cDNA HR4 CTCATCAGCGGTATCCGTGGTGACATCCAGTACATCAATGACGAGCTTGCCAGCATGCAG 180
Pikp-2 cds K60 CTCATCAGCGGTATCCGTGGTGACATCCAGTACATCAATGACGAGCTTGCCAGCATGCAG 144
Pikh-2 cds K3 CTCATCAGCGGTATCCGTGGTGACATCCAGTACATCAATGACGAGCTTGCCAGCATGCAG 144
Pik2 cds Kanto51 CTCATCAGCGGTATCCGTGGTGACATCCAGTACATCAATGACGAGCTTGCCAGCATGCAG 144
Pikm2 cds Tsuyuake CTCATCAGCGGTATCCGTGGTGACATCCAGTACATCAATGACGAGCTTGCCAGCATGCAG 144
************************************************************
Pi-h1_t_-2 cDNA HR4 GCCTTCCTCCGTGATCTCAGCAACGTGCCAGAGGGTCACAGTCATGGCCACCGGATGAAG 240
Pikp-2 cds K60 GCCTTCCTCCGTGATCTCAGCAACGTGCCAGAGGGTCACAGTCATGGCCACCGGATGAAG 204
Pikh-2 cds K3 GCCTTCCTCCGTGATCTCAGCAACGTGCCAGAGGGTCACAGTCATGGCCACCGGATGAAG 204
Pik2 cds Kanto51 GCCTTCCTCCGTGATCTCAGCAACGTGCCAGAGGGTCACAGTCATGGCCACCGGATGAAG 204
Pikm2 cds Tsuyuake GCCTTCCTCCGTGATCTCAGCAACGTGCCAGAGGGTCACAGTCATGGCCACCGGATGAAG 204
************************************************************
Pi-h1_t_-2 cDNA HR4 GACTGGATGAAGCAGATCCGAGACATCGCCTATGATGTTGAGGACTGTATCGATGACTTT 300
Pikp-2 cds K60 GACTGGATGAAGCAGATCCGAGACATCGCCTATGATGTTGAGGACTGTATCGATGACTTT 264
Pikh-2 cds K3 GACTGGATGAAGCAGATCCGAGACATCGCCTATGATGTTGAGGACTGTATCGATGACTTT 264
Pik2 cds Kanto51 GACTGGATGAAGCAGATCCGAGACATCGCCTATGATGTTGAGGACTGTATCGATGACTTT 264
Pikm2 cds Tsuyuake GACTGGATGAAGCAGATCCGAGACATCGCCTATGATGTTGAGGACTGTATCGATGACTTT 264
************************************************************
Pi-h1_t_-2 cDNA HR4 GCCCACCGCCTCCCTCAGGATTCCATCAGCGATGCCAAATGGTCCTTCCTACTCACAAAA 360
Pikp-2 cds K60 GCCCACCGCCTCCCTCAGGATTCCATCAGCGATGCCAAATGGTCCTTCCTACTCACAAAA 324
Pikh-2 cds K3 GCCCACCGCCTCCCTCAGGATTCCATCAGCGATGCCAAATGGTCCTTCCTACTCACAAAA 324
Pik2 cds Kanto51 GCCCACCGCCTCCCTCAGGATTCCATCAGCGATGCCAAATGGTCCTTCCTACTCACAAAA 324
Pikm2 cds Tsuyuake GCCCACCGCCTCCCTCAGGATTCCATCAGCGATGCCAAATGGTCCTTCCTACTCACAAAA 324
************************************************************
Pi-h1_t_-2 cDNA HR4 ATCTATGAACTATGGACATGGTGGCCACGTCGTGTGATTGCTTCCAACATTGCCCAACTC 420
Pikp-2 cds K60 ATCTATGAACTATGGACATGGTGGCCACGTCGTGTGATTGCTTCCAACATTGCCCAACTC 384
Pikh-2 cds K3 ATCTATGAACTATGGACATGGTGGCCACGTCGTGTGATTGCTTCCAACATTGCCCAACTC 384
Pik2 cds Kanto51 ATCTATGAACTATGGACATGGTGGCCACGTCGTGTGATTGCTTCCAACATTGCCCAACTC 384
Pikm2 cds Tsuyuake ATCTATGAACTATGGACATGGTGGCCACGTCGTGTGATTGCTTCCAACATTGCCCAACTC 384
************************************************************
Pi-h1_t_-2 cDNA HR4 AAGGTACGGGCACAACAGATCGCAGATCGACGTAGTAGATACGGAGTGAACAACCCAGAA 480
Pikp-2 cds K60 AAGGTACGGGCACAACAGATCGCAGATCGACGTAGTAGATACGGAGTGAACAACCCAGAA 444
Pikh-2 cds K3 AAGGTACGGGCACAACAGATCGCAGATCGACGTAGTAGATACGGAGTGAACAACCCAGAA 444
Pik2 cds Kanto51 AAGGTACGGGCACAACAGATCGCAGATCGACGTAGTAGATACGGAGTGAACAACCCAGAA 444
Pikm2 cds Tsuyuake AAGGTACGGGCACAACAGATCGCAGATCGACGTAGTAGATACGGAGTGAACAACCCAGAA 444
************************************************************
Pi-h1_t_-2 cDNA HR4 CACCTTGACAGTAGCAGCAGTGCCAGGACCCGTGCTGTCAATTACGAAATTGCTGAGTAT 540
Pikp-2 cds K60 CACCTTGACAGTAGCAGCAGTGCCAGGACCCGTGCTGTCAATTACGAAATTGCTGAGTAT 504
Pikh-2 cds K3 CACCTTGACAGTAGCAGCAGTGCCAGGACCCGTGCTGTCAATTACGAAATTGCTGAGTAT 504
Pik2 cds Kanto51 CACCTTGACAGTAGCAGCAGTGCCAGGACCCGTGCTGTCAATTACGAAATTGCTGAGTAT 504
Pikm2 cds Tsuyuake CACCTTGACAGTAGCAGCAGTGCCAGGACCCGTGCTGTCAATTACGAAATTGCTGAGTAT 504
************************************************************
Pi-h1_t_-2 cDNA HR4 CAGGTCACAAGCCCTCAGATCATTGGTATAAAGGAGCCTGTGGGGATGAAGACGGTCATG 600
Pikp-2 cds K60 CAGGTCACAAGCCCTCAGATCATTGGTATAAAGGAGCCTGTGGGGATGAAGACGGTCATG 564
Pikh-2 cds K3 CAGGTCACAAGCCCTCAGATCATTGGTATAAAGGAGCCTGTGGGGATGAAGACGGTCATG 564
Pik2 cds Kanto51 CAGGTCACAAGCCCTCAGATCATTGGTATAAAGGAGCCTGTGGGGATGAAGACGGTCATG 564
Pikm2 cds Tsuyuake CAGGTCACAAGCCCTCAGATCATTGGTATAAAGGAGCCTGTGGGGATGAAGACGGTCATG 564
************************************************************
Pi-h1_t_-2 cDNA HR4 GAGGAGCTTGAGGTTTGGTTAACTAATCCTCAAGCTGAAAATGGGCAAGCTGTTCTGTCC 660
Pikp-2 cds K60 GAGGAGCTTGAGGTTTGGTTAACTAATCCTCAAGCTGAAAATGGGCAAGCTGTTCTGTCC 624
Pikh-2 cds K3 GAGGAGCTTGAGGTTTGGTTAACTAATCCTCAAGCTGAAAATGGGCAAGCTGTTCTGTCC 624
Pik2 cds Kanto51 GAGGAGCTTGAGGTTTGGTTAACTAATCCTCAAGCTGAAAATGGGCAAGCTGTTCTGTCC 624
Pikm2 cds Tsuyuake GAGGAGCTTGAGGTTTGGTTAACTAATCCTCAAGCTGAAAATGGGCAAGCTGTTCTGTCC 624
************************************************************
Pi-h1_t_-2 cDNA HR4 ATAGTCGGTTTTGGAGGTGTGGGAAAGACTACCATTGCCACAGCATTGTACAGAAAAGTC 720
Pikp-2 cds K60 ATAGTCGGTTTTGGAGGTGTGGGAAAGACTACCATTGCCACAGCATTGTACAGAAAAGTC 684
Pikh-2 cds K3 ATAGTCGGTTTTGGAGGTGTGGGAAAGACTACCATTGCCACAGCATTGTACAGAAAAGTC 684
Pik2 cds Kanto51 ATAGTCGGTTTTGGAGGTGTGGGAAAGACTACCATTGCCACAGCATTGTACAGAAAAGTC 684
Pikm2 cds Tsuyuake ATAGTCGGTTTTGGAGGTGTGGGAAAGACTACCATTGCCACAGCATTGTACAGAAAAGTC 684
************************************************************
Pi-h1_t_-2 cDNA HR4 AGTGATAAATTTCAGTGCCGGGCATCAGTAGCTGTGTCTCAGAACTATGACCAAGGCAAA 780
Pikp-2 cds K60 AGTGATAAATTTCAGTGCCGGGCATCAGTAGCTGTGTCTCAGAACTATGACCAAGGCAAA 744
Pikh-2 cds K3 AGTGATAAATTTCAGTGCCGGGCATCAGTAGCTGTGTCTCAGAACTATGACCAAGGCAAA 744
Pik2 cds Kanto51 AGTGAAAAATTTCAGTGCCGGGCATCAGTAGCTGTGTCTCAGAACTATGACCAAGGCAAA 744
Pikm2 cds Tsuyuake AGTGAAAAATTTCAGTGCCGGGCATCAGTAGCTGTGTCTCAGAACTATGACCAAGGCAAA 744
***** ******************************************************
Pi-h1_t_-2 cDNA HR4 GTCCTCAATAGTATTCTGAGTCAAGTCAGCAATCAGGAGCAGGGCAGCAGCACAACAATT 840
Pikp-2 cds K60 GTCCTCAATAGTATTCTGAGTCAAGTCAGCAATCAGGAGCAGGGCAGCAGCACAACAATT 804
Pikh-2 cds K3 GTCCTCAATAGTATTCTGAGTCAAGTCAGCAATCAGGAGCAGGGCAGCAGCACAACAATT 804
Pik2 cds Kanto51 GTCCTCAATAGTATTCTGAGTCAAGTCAGCAATCAGGAGCAGGGCAGCAGCACAACAATT 804
Pikm2 cds Tsuyuake GTCCTCAATAGTATTCTGAGTCAAGTCAGCAATCAGGAGCAGGGCAGCAGCACAACAATT 804
************************************************************
Pi-h1_t_-2 cDNA HR4 AGTGAGAAAAAGAACCTCACCTCAGGCGCTAAGAGCATGTTGAAGACAGCCCTGTCACTG 900
Pikp-2 cds K60 AGTGAGAAAAAGAACCTCACCTCAGGCGCTAAGAGCATGTTGAAGACAGCCCTGTCACTG 864
Pikh-2 cds K3 AGTGAGAAAAAGAACCTCACCTCAGGCGCTAAGAGCATGTTGAAGACAGCCCTGTCACTG 864
Pik2 cds Kanto51 AGTGAGAAAAAGAACCTCACCTCAGGCGCTAAGAGCATGTTGAAGACAGCCCTGTCACTG 864
Pikm2 cds Tsuyuake AGTGAGAAAAAGAACCTCACCTCAGGCGCTAAGAGCATGTTGAAGACAGCCCTGTCACTG 864
************************************************************
Pi-h1_t_-2 cDNA HR4 CTCAGAGGTAATTGTATATGTCAGCCAGAAAATGATGGAAACCCTGATAATACACCAATC 960
Pikp-2 cds K60 CTCAGAGGTAATTGTATATGTCAGCCAGAAAATGATGGAAACCCTGATAATACACCAATC 924
Pikh-2 cds K3 CTCAGAGGTAATTGTATATGTCAGCCAGAAAATGATGGAAACCCTGATAATACACCAATC 924
Pik2 cds Kanto51 CTCAGAGGTAATTGTATATGTCAGCCAGAAAATGATGGAAACCCTGATAATACACCAATC 924
Pikm2 cds Tsuyuake CTCAGAGGTAATTGTATATGTCAGCCAGAAAATGATGGAAACCCTGATAATACACCAATC 924
************************************************************
Pi-h1_t_-2 cDNA HR4 AGGCTGCAGGAAACAACGGACGATGATCAAAACCCCAGAAAACTGGAACAGCTCCTGGCC 1020
Pikp-2 cds K60 AGGCTGCAGGAAACAACGGACGATGATCAAAACCCCAGAAAACTGGAACAGCTCCTGGCC 984
Pikh-2 cds K3 AGGCTGCAGGAAACAACGGACGATGATCAAAACCCCAGAAAACTGGAACAGCTCCTGGCC 984
Pik2 cds Kanto51 AGGCTGCAGGAAACAACGGACGATGATCAAAACCCCAGAAAACTGGAACAGCTCCTGGCC 984
Pikm2 cds Tsuyuake AGGCTGCAGGAAACAACGGACGATGATCAAAACCCCAGAAAACTGGAACAGCTCCTGGCC 984
************************************************************
Pi-h1_t_-2 cDNA HR4 GAAAAGAGTTATATCCTCTTGATTGATGACATTTGGTCTGCCGAAACATGGGAGAGTATC 1080
Pikp-2 cds K60 GAAAAGAGTTATATCCTCTTGATTGATGACATTTGGTCTGCCGAAACATGGGAGAGTATC 1044
Pikh-2 cds K3 GAAAAGAGTTATATCCTCTTGATTGATGACATTTGGTCTGCCGAAACATGGGAGAGTATC 1044
Pik2 cds Kanto51 GAAAAGAGTTATATCCTCTTGATTGATGACATTTGGTCTGCCGAAACATGGGAGAGTATC 1044
Pikm2 cds Tsuyuake GAAAAGAGTTATATCCTCTTGATTGATGACATTTGGTCTGCCGAAACATGGGAGAGTATC 1044
************************************************************
Pi-h1_t_-2 cDNA HR4 AGATCGATTTTGCCTAAAAATAATAAAGGCGGTAGAATAATAGTGACTACAAGATTTCAA 1140
Pikp-2 cds K60 AGATCGATTTTGCCTAAAAATAATAAAGGCGGTAGAATAATAGTGACTACAAGATTTCAA 1104
Pikh-2 cds K3 AGATCGATTTTGCCTAAAAATAATAAAGGCGGTAGAATAATAGTGACTACAAGATTTCAA 1104
Pik2 cds Kanto51 AGATCGATTTTGCCTAAAAATAATAAAGGCGGTAGAATAATAGTGACTACAAGATTTCAA 1104
Pikm2 cds Tsuyuake AGATCGATTTTGCCTAAAAATAATAAAGGCGGTAGAATAATAGTGACTACAAGATTTCAA 1104
************************************************************
Pi-h1_t_-2 cDNA HR4 GCTGTTGGTTCAACATGCTCCCCTCTTGAAACTGATCGTTTGCATACAGTTGATTTTCTC 1200
Pikp-2 cds K60 GCTGTTGGTTCAACATGCTCCCCTCTTGAAACTGATCGTTTGCATACAGTTGATTTTCTC 1164
Pikh-2 cds K3 GCTGTTGGTTCAACATGCTCCCCTCTTGAAACTGATCGTTTGCATACAGTTGATTTTCTC 1164
Pik2 cds Kanto51 GCTGTTGGTTCAACATGCTCCCCTCTTGAAACTGATCGTTTGCATACAGTTGATTTTCTC 1164
Pikm2 cds Tsuyuake GCTGTTGGTTCAACATGCTCCCCTCTTGAAACTGATCGTTTGCATACAGTTGATTTTCTC 1164
************************************************************
Pi-h1_t_-2 cDNA HR4 ACCGATGACGAGTCCCAAAACTTATTCAATACAAGTATTTGTGAATCAAAGATAAGAAAA 1260
Pikp-2 cds K60 ACCGATGACGAGTCCCAAAACTTATTCAATACAAGTATTTGTGAATCAAAGATAAGAAAA 1224
Pikh-2 cds K3 ACCGATGACGAGTCCCAAAACTTATTCAATACAAGTATTTGTGAATCAAAGATAAGAAAA 1224
Pik2 cds Kanto51 ACCGATGACGAGTCCCAAAACTTATTCAATACAAGTATTTGTGAATCAAAGATAAGAAAA 1224
Pikm2 cds Tsuyuake ACCGATGACGAGTCCCAAAACTTATTCAATACAAGTATTTGTGAATCAAAGATAAGAAAA 1224
************************************************************
Pi-h1_t_-2 cDNA HR4 GATAGCAACAAAGTAGACGAGCAAGTCCCTGAGGAAATATGGAAAATATGTGGGGGATTG 1320
Pikp-2 cds K60 GATAGCAACAAAGTAGACGAGCAAGTCCCTGAGGAAATATGGAAAATATGTGGGGGATTG 1284
Pikh-2 cds K3 GATAGCAACAAAGTAGACGAGCAAGTCCCTGAGGAAATATGGAAAATATGTGGGGGATTG 1284
Pik2 cds Kanto51 GATAGCAACAAAGTAGACGAGCAAGTCCCTGAGGAAATATGGAAAATATGTGGGGGATTG 1284
Pikm2 cds Tsuyuake GATAGCAACAAAGTAGACGAGCAAGTCCCTGAGGAAATATGGAAAATATGTGGGGGATTG 1284
************************************************************
Pi-h1_t_-2 cDNA HR4 CCTTTGGCCATAGTCACCATGGCTGGTCTTGTCGCCTGCAACCCAAGGAAAGCCTGCTGC 1380
Pikp-2 cds K60 CCTTTGGCCATAGTCACCATGGCTGGTCTTGTCGCCTGCAACCCAAGGAAAGCCTGCTGC 1344
Pikh-2 cds K3 CCTTTGGCCATAGTCACCATGGCTGGTCTTGTCGCCTGCAACCCAAGGAAAGCCTGCTGC 1344
Pik2 cds Kanto51 CCTTTGGCCATAGTCAGCATGGCTGGTCTTGTCGCCTGCAACCCAAGGAAAGCCTGCTGC 1344
Pikm2 cds Tsuyuake CCTTTGGCCATAGTCAGCATGGCTGGTCTTGTCGCCTGCAACCCAAGGAAAGCCTGCTGC 1344
**************** *******************************************
Pi-h1_t_-2 cDNA HR4 GATTGGAGTAAACTTTGCAAATCATTATTTCCAGAGCAAGAAACTCCTCTTACCCTCGAT 1440
Pikp-2 cds K60 GATTGGAGTAAACTTTGCAAATCATTATTTCCAGAGCAAGAAACTCCTCTTACCCTCGAT 1404
Pikh-2 cds K3 GATTGGAGTAAACTTTGCAAATCATTATTTCCAGAGCAAGAAACTCCTCTTACCCTCGAT 1404
Pik2 cds Kanto51 GATTGGAGTAAACTTTGCAAATCATTATTTCCAGAGCAAGAAACTCCTCTTACCCTCGAT 1404
Pikm2 cds Tsuyuake GATTGGAGTAAACTTTGCAAATCATTATTTCCAGAGCAAGAAACTCCTCTTACCCTCGAT 1404
************************************************************
Pi-h1_t_-2 cDNA HR4 GGTGTTACAAGGATACTGGATTGTTGTTACAATGATTTGCCTGCGGATCTGAAGACTTGC 1500
Pikp-2 cds K60 GGTGTTACAAGGATACTGGATTGTTGTTACAATGATTTGCCTGCGGATCTGAAGACTTGC 1464
Pikh-2 cds K3 GGTGTTACAAGGATACTGGATTGTTGTTACAATGATTTGCCTGCGGATCTGAAGACTTGC 1464
Pik2 cds Kanto51 GGTGTTACAAGGATACTGGATTGTTGTTACAATGATTTGCCTGCGGATCTGAAGACTTGC 1464
Pikm2 cds Tsuyuake GGTGTTACAAGGATACTGGATTGTTGTTACAATGATTTGCCTGCGGATCTGAAGACTTGC 1464
************************************************************
Pi-h1_t_-2 cDNA HR4 TTATTGTACTTGAGTATATTTCCGAAGGGTTGGAAAATTAGTAGGAAACGTTTGTCCCGG 1560
Pikp-2 cds K60 TTATTGTACTTGAGTATATTTCCGAAGGGTTGGAAAATTAGTAGGAAACGTTTGTCCCGG 1524
Pikh-2 cds K3 TTATTGTACTTGAGTATATTTCCGAAGGGTTGGAAAATTAGTAGGAAACGTTTGTCCCGG 1524
Pik2 cds Kanto51 TTATTGTACTTGAGTATATTTCCGAAGGGTTGGAAAATTAGTAGGAAACGTTTGTCCCGG 1524
Pikm2 cds Tsuyuake TTATTGTACTTGAGTATATTTCCGAAGGGTTGGAAAATTAGTAGGAAACGTTTGTCCCGG 1524
************************************************************
Pi-h1_t_-2 cDNA HR4 CGATGGATAGCTGAAGGTTTTGCTAATGAGAAGCAAGGGTTAACCCAGGAAAGAGTTGCA 1620
Pikp-2 cds K60 CGATGGATAGCTGAAGGTTTTGCTAATGAGAAGCAAGGGTTAACCCAGGAAAGAGTTGCA 1584
Pikh-2 cds K3 CGATGGATAGCTGAAGGTTTTGCTAATGAGAAGCAAGGGTTAACCCAGGAAAGAGTTGCA 1584
Pik2 cds Kanto51 CGATGGATAGCTGAAGGTTTTGCTAATGAGAAGCAAGGGTTAACCCAGGAAAGAGTTGCA 1584
Pikm2 cds Tsuyuake CGATGGATAGCTGAAGGTTTTGCTAATGAGAAGCAAGGGTTAACCCAGGAAAGAGTTGCA 1584
************************************************************
Pi-h1_t_-2 cDNA HR4 GAGGCATACTTTAATCAACTCACAAGAAGGAACTTAGTACGTCCCATGGAGCATGGCAGC 1680
Pikp-2 cds K60 GAGGCATACTTTAATCAACTCACAAGAAGGAACTTAGTACGTCCCATGGAGCATGGCAGC 1644
Pikh-2 cds K3 GAGGCATACTTTAATCAACTCACAAGAAGGAACTTAGTACGTCCCATGGAGCATGGCAGC 1644
Pik2 cds Kanto51 GAGGCATACTTTAATCAACTCACAAGAAGGAACTTAGTACGTCCCATGGAGCATGGCAGC 1644
Pikm2 cds Tsuyuake GAGGCATACTTTAATCAACTCACAAGAAGGAACTTAGTACGTCCCATGGAGCATGGCAGC 1644
************************************************************
Pi-h1_t_-2 cDNA HR4 AATGGGAAGGTAAAAACGTTTCAAGTTCATGACATGGTTCTTGAATACATCATGTCCAAA 1740
Pikp-2 cds K60 AATGGGAAGGTAAAAACGTTTCAAGTTCATGACATGGTTCTTGAATACATCATGTCCAAA 1704
Pikh-2 cds K3 AATGGGAAGGTAAAAACGTTTCAAGTTCATGACATGGTTCTTGAATACATCATGTCCAAA 1704
Pik2 cds Kanto51 AATGGGAAGGTAAAAACGTTTCAAGTTCATGACATGGTTCTTGAATACATCATGTCCAAA 1704
Pikm2 cds Tsuyuake AATGGGAAGGTAAAAACGTTTCAAGTTCATGACATGGTTCTTGAATACATCATGTCCAAA 1704
************************************************************
Pi-h1_t_-2 cDNA HR4 TCAATCGAAGAGAATTTTATTACTGTGGTTGGTGGACACTGGCAGATGACTGCACCAAGC 1800
Pikp-2 cds K60 TCAATCGAAGAGAATTTTATTACTGTGGTTGGTGGACACTGGCAGATGACTGCACCAAGC 1764
Pikh-2 cds K3 TCAATCGAAGAGAATTTTATTACTGTGGTTGGTGGACACTGGCAGATGACTGCACCAAGC 1764
Pik2 cds Kanto51 TCAATCGAAGAGAATTTTATTACTGTGGTTGGTGGACACTGGCAGATGACTGCACCAAGC 1764
Pikm2 cds Tsuyuake TCAATCGAAGAGAATTTTATTACTGTGGTTGGTGGACACTGGCAGATGACTGCACCAAGC 1764
************************************************************
Pi-h1_t_-2 cDNA HR4 AATAAAGTCCGTCGACTGTCGATGCAAAGCAGTGGATCCAATCGTGGAAGTTCAACAAAA 1860
Pikp-2 cds K60 AATAAAGTCCGTCGACTGTCGATGCAAAGCAGTGGATCCAATCGTGGAAGTTCAACAAAA 1824
Pikh-2 cds K3 AATAAAGTCCGTCGACTGTCGATGCAAAGCAGTGGATCCAATCGTGGAAGTTCAACAAAA 1824
Pik2 cds Kanto51 AATAAAGTCCGTCGACTGTCGATGCAAAGCAGTGGATCCAATCGTGGAAGTTCAACAAAA 1824
Pikm2 cds Tsuyuake AATAAAGTCCGTCGACTGTCGATGCAAAGCAGTGGATCCAATCGTGGAAGTTCAACAAAA 1824
************************************************************
Pi-h1_t_-2 cDNA HR4 GGCCTGAACTTGGCTCAAGTGAGATCACTGACGGTGTTTGGGAACCTGAACCATATGCCA 1920
Pikp-2 cds K60 GGCCTGAACTTGGCTCAAGTGAGATCACTGACGGTGTTTGGGAACCTGAACCATATGCCA 1884
Pikh-2 cds K3 GGCCTGAACTTGGCTCAAGTGAGATCACTGACGGTGTTTGGGAACCTGAACCATATGCCA 1884
Pik2 cds Kanto51 GGCCTGAACTTGGCTCAAGTGAGATCACTGACGGTGTTTGGGAACCTGAACCATGTGCCA 1884
Pikm2 cds Tsuyuake GGCCTGAACTTGGCTCAAGTGAGATCACTGACGGTGTTTGGGAACCTGAACCATGTGCCA 1884
****************************************************** *****
Pi-h1_t_-2 cDNA HR4 TTCCATTCATTCAACTATGGGATAATACAGGTGCTGGATCTTGAGGACTGGAAGGGTTTG 1980
Pikp-2 cds K60 TTCCATTCATTCAACTATGGGATAATACAGGTGCTGGATCTTGAGGACTGGAAGGGTTTG 1944
Pikh-2 cds K3 TTCCATTCATTCAACTATGGGATAATACAGGTGCTGGATCTTGAGGACTGGAAGGGTTTG 1944
Pik2 cds Kanto51 TTCCATTCATTCAACTATGGGATAATACAGGTGCTGGATCTTGAGGACTGGAAGGGTTTG 1944
Pikm2 cds Tsuyuake TTCCATTCATTCAACTATGGGATAATACAGGTGCTGGATCTTGAGGACTGGAAGGGTTTG 1944
************************************************************
Pi-h1_t_-2 cDNA HR4 AAAGAGAGACATATGACGGAGATATGTCAAATGCTTTTACTCAAGTATTTGAGCATCCGA 2040
Pikp-2 cds K60 AAAGAGAGACATATGACGGAGATATGTCAAATGCTTTTACTCAAGTATTTGAGCATCCGA 2004
Pikh-2 cds K3 AAAGAGAGACATATGACGGAGATATGTCAAATGCTTTTACTCAAGTATTTGAGCATCCGA 2004
Pik2 cds Kanto51 AAAGAGAGACATATGACGGAGATATGTCAAATGCTTTTACTCAAGTATTTGAGCATCCGA 2004
Pikm2 cds Tsuyuake AAAGAGAGACATATGACGGAGATATGTCAAATGCTTTTACTCAAGTATTTGAGCATCCGA 2004
************************************************************
Pi-h1_t_-2 cDNA HR4 CGAACAGAAATTTCCAAAATTCCCTCCAAGATTCAGAAACTTGAGTACTTGGAAACTCTT 2100
Pikp-2 cds K60 CGAACAGAAATTTCCAAAATTCCCTCCAAGATTCAGAAACTTGAGTACTTGGAAACTCTT 2064
Pikh-2 cds K3 CGAACAGAAATTTCCAAAATTCCCTCCAAGATTCAGAAACTTGAGTACTTGGAAACTCTT 2064
Pik2 cds Kanto51 CGAACAGAAATTTCCAAAATTCCCTCCAAGATTCAGAAACTTGAGTACTTGGAAACTCTT 2064
Pikm2 cds Tsuyuake CGAACAGAAATTTCCAAAATTCCCTCCAAGATTCAGAAACTTGAGTACTTGGAAACTCTT 2064
************************************************************
Pi-h1_t_-2 cDNA HR4 GACATAAGGGAGACATATGTCAGGGACCTGCCTAAGTCAATAGTCCAGCTAAAACGGATC 2160
Pikp-2 cds K60 GACATAAGGGAGACATATGTCAGGGACCTGCCTAAGTCAATAGTCCAGCTAAAACGGATC 2124
Pikh-2 cds K3 GACATAAGGGAGACATATGTCAGGGACCTGCCTAAGTCAATAGTCCAGCTAAAACGGATC 2124
Pik2 cds Kanto51 GACATAAGGGAGACATATGTCAGGGACCTGCCTAAGTCAATAGTCCAGCTAAAACGGATC 2124
Pikm2 cds Tsuyuake GACATAAGGGAGACATATGTCAGGGACCTGCCTAAGTCAATAGTCCAGCTAAAACGGATC 2124
************************************************************
Pi-h1_t_-2 cDNA HR4 ATTAGCATACTTGGAGGGAATAAAAACACACGGAAGGGGCTGAGGTTGCCTCAAGAAAAA 2220
Pikp-2 cds K60 ATTAGCATACTTGGAGGGAATAAAAACACACGGAAGGGGCTGAGGTTGCCTCAAGAAAAA 2184
Pikh-2 cds K3 ATTAGCATACTTGGAGGGAATAAAAACACACGGAAGGGGCTGAGGTTGCCTCAAGAAAAA 2184
Pik2 cds Kanto51 ATTAGCATACTTGGAGGGAATAAAAACACACGGAAGGGGCTGAGGTTGCCTCAAGAAAAA 2184
Pikm2 cds Tsuyuake ATTAGCATACTTGGAGGGAATAAAAACACACGGAAGGGGCTGAGGTTGCCTCAAGAAAAA 2184
************************************************************
Pi-h1_t_-2 cDNA HR4 AGTAAGAAGCCAATTAAAAACCCGTCGCCTCAAGGAAAAACAAAGGAGCCCGCAAAGAAA 2280
Pikp-2 cds K60 AGTAAGAAGCCAATTAAAAACCCGTCGCCTCAAGGAAAAACAAAGGAGCCCGCAAAGAAA 2244
Pikh-2 cds K3 AGTAAGAAGCCAATTAAAAACCCGTCGCCTCAAGGAAAAACAAAGGAGCCCGCAAAGAAA 2244
Pik2 cds Kanto51 AGTAAGAAGCCAATTAAAAACCCGTCGCCTCAAGGAAAAACAAAGGAGCCCGCAAAGAAA 2244
Pikm2 cds Tsuyuake AGTAAGAAGCCAATTAAAAACCCGTCGCCTCAAGGAAAAACAAAGGAGCCCGCAAAGAAA 2244
************************************************************
Pi-h1_t_-2 cDNA HR4 GGATTCTTATCCCAAGAAAAAGGTAAAGGCGCAATGAAAGCACTCCGTGTACTGTCAGGG 2340
Pikp-2 cds K60 GGATTCTTATCCCAAGAAAAAGGTAAAGGCGCAATGAAAGCACTCCGTGTACTGTCAGGG 2304
Pikh-2 cds K3 GGATTCTTATCCCAAGAAAAAGGTAAAGGCGCAATGAAAGCACTCCGTGTACTGTCAGGG 2304
Pik2 cds Kanto51 GGATTCTTATCCCAAGAAAAAGGTAAAGGCGCAATGAAAGCACTCCGTGTACTGTCAGGG 2304
Pikm2 cds Tsuyuake GGATTCTTATCCCAAGAAAAAGGTAAAGGCGCAATGAAAGCACTCCGTGTACTGTCAGGG 2304
************************************************************
Pi-h1_t_-2 cDNA HR4 ATTGAGATTGTTGAGGAATCATCAGAAGTAGCTGCAGGCCTTCATCAGTTGACAGGGCTA 2400
Pikp-2 cds K60 ATTGAGATTGTTGAGGAATCATCAGAAGTAGCTGCAGGCCTTCATCAGTTGACAGGGCTA 2364
Pikh-2 cds K3 ATTGAGATTGTTGAGGAATCATCAGAAGTAGCTGCAGGCCTTCATCAGTTGACAGGGCTA 2364
Pik2 cds Kanto51 ATTGAGATTGTTGAGGAATCATCAGAAGTAGCTGCAGGCCTTCATCAGTTGACAGGGCTA 2364
Pikm2 cds Tsuyuake ATTGAGATTGTTGAGGAATCATCAGAAGTAGCTGCAGGCCTTCATCAGTTGACAGGGCTA 2364
************************************************************
Pi-h1_t_-2 cDNA HR4 AGGAAGCTTGCCATATACAAGCTCAATATAACAAAGGGTGGTGATACCTTCAAACAATTA 2460
Pikp-2 cds K60 AGGAAGCTTGCCATATACAAGCTCAATATAACAAAGGGTGGTGATACCTTCAAACAATTA 2424
Pikh-2 cds K3 AGGAAGCTTGCCATATACAAGCTCAATATAACAAAGGGTGGTGATACCTTCAAACAATTA 2424
Pik2 cds Kanto51 AGGAAGCTTGCCATATACAAGCTCAATATAACAAAGGGTGGTGATACCTTCAAACAATTA 2424
Pikm2 cds Tsuyuake AGGAAGCTTGCCATATACAAGCTCAATATAACAAAGGGTGGTGATACCTTCAAACAATTA 2424
************************************************************
Pi-h1_t_-2 cDNA HR4 CAGTCCTCCATTGAGTACCTTGGCAGCTGTGGTCTGCAGACTCTGGCCATCAATGATGAG 2520
Pikp-2 cds K60 CAGTCCTCCATTGAGTACCTTGGCAGCTGTGGTCTGCAGACTCTGGCCATCAATGATGAG 2484
Pikh-2 cds K3 CAGTCCTCCATTGAGTACCTTGGCAGCTGTGGTCTGCAGACTCTGGCCATCAATGATGAG 2484
Pik2 cds Kanto51 CAGTCCTCCATTGAGTACCTTGGCAGCTGTGGTCTGCAGACTCTGGCCATCAATGATGAG 2484
Pikm2 cds Tsuyuake CAGTCCTCCATTGAGTACCTTGGCAGCTGTGGTCTGCAGACTCTGGCCATCAATGATGAG 2484
************************************************************
Pi-h1_t_-2 cDNA HR4 AATTCTGAATTTATCAACTCACTGGGCGACATGCCCGCGCCTCCAAGATATCTTGTCGCC 2580
Pikp-2 cds K60 AATTCTGAATTTATCAACTCACTGGGCGACATGCCCGCGCCTCCAAGATATCTTGTCGCC 2544
Pikh-2 cds K3 AATTCTGAATTTATCAACTCACTGGGCGACATGCCCGCGCCTCCAAGATATCTTGTCGCC 2544
Pik2 cds Kanto51 AATTCTGAATTTATCAACTCACTGGGCGACATGCCCGCGCCTCCAAGATATCTTGTCGCC 2544
Pikm2 cds Tsuyuake AATTCTGAATTTATCAACTCACTGGGCGACATGCCCGCGCCTCCAAGATATCTTGTCGCC 2544
************************************************************
Pi-h1_t_-2 cDNA HR4 CTTGAGCTGTCTGGCAAGTTGGAGAAGCTACCCAAGTGGATCACCAGCATCACTACTCTC 2640
Pikp-2 cds K60 CTTGAGCTGTCTGGCAAGTTGGAGAAGCTACCCAAGTGGATCACCAGCATCACTACTCTC 2604
Pikh-2 cds K3 CTTGAGCTGTCTGGCAAGTTGGAGAAGCTACCCAAGTGGATCACCAGCATCACTACTCTC 2604
Pik2 cds Kanto51 CTTGAGCTGTCTGGCAAGTTGGAGAAGCTACCCAAGTGGATCACCAGCATCACTACTCTC 2604
Pikm2 cds Tsuyuake CTTGAGCTGTCTGGCAAGTTGGAGAAGCTACCCAAGTGGATCACCAGCATCACTACTCTC 2604
************************************************************
Pi-h1_t_-2 cDNA HR4 AACAAGCTAACCATATCTGTAACAGTTCTTAGGACTGAAACTTTGGAGATCCTCCACATT 2700
Pikp-2 cds K60 AACAAGCTAACCATATCTGTAACAGTTCTTAGGACTGAAACTTTGGAGATCCTCCACATT 2664
Pikh-2 cds K3 AACAAGCTAACCATATCTGTAACAGTTCTTAGGACTGAAACTTTGGAGATCCTCCACATT 2664
Pik2 cds Kanto51 AACAAGCTAACCATATCTGTAACAGTTCTTAGGACTGAAACTTTGGAGATCCTCCACATT 2664
Pikm2 cds Tsuyuake AACAAGCTAACCATATCTGTAACAGTTCTTAGGACTGAAACTTTGGAGATCCTCCACATT 2664
************************************************************
Pi-h1_t_-2 cDNA HR4 TTACCTTCATTGTTTTCCCTCACCTTCGCCTTTTCACTTAGTGCAGCGAAGCAGGATCAG 2760
Pikp-2 cds K60 TTACCTTCATTGTTTTCCCTCACCTTCGCCTTTTCACTTAGTGCAGCGAAGCAGGATCAG 2724
Pikh-2 cds K3 TTACCTTCATTGTTTTCCCTCACCTTCGCCTTTTCACTTAGTGCAGCGAAGCAGGATCAG 2724
Pik2 cds Kanto51 TTACCTTCATTGTTTTCCCTCACCTTCGCCTTTTCACTTAGTGCAGCGAAGCAGGATCAG 2724
Pikm2 cds Tsuyuake TTACCTTCATTGTTTTCCCTCACCTTCGCCTTTTCACTTAGTGCAGCGAAGCAGGATCAG 2724
************************************************************
Pi-h1_t_-2 cDNA HR4 GACATAATAAAGGACATCCTTGAGAATAATAAATTGGACAGTGATGGGGAAATCGTCATT 2820
Pikp-2 cds K60 GACATAATAAAGGACATCCTTGAGAATAATAAATTGGACAGTGATGGGGAAATCGTCATT 2784
Pikh-2 cds K3 GACATAATAAAGGACATCCTTGAGAATAATAAATTGGACAGTGATGGGGAAATCGTCATT 2784
Pik2 cds Kanto51 GACATAATAAAGGACATCCTTGAGAATAATAAATTGGACAGTGATGGGGAAATCGTCATT 2784
Pikm2 cds Tsuyuake GACATAATAAAGGACATCCTTGAGAATAATAAATTGGACAGTGATGGGGAAATCGTCATT 2784
************************************************************
Pi-h1_t_-2 cDNA HR4 CCAGCTGAAGGATTCAAGAGTCTTAAGCTGCTTCGCTTCTTTGCACCTTTAGTGCCGAAG 2880
Pikp-2 cds K60 CCAGCTGAAGGATTCAAGAGTCTTAAGCTGCTTCGCTTCTTTGCACCTTTAGTGCCGAAG 2844
Pikh-2 cds K3 CCAGCTGAAGGATTCAAGAGTCTTAAGCTGCTTCGCTTCTTTGCACCTTTAGTGCCGAAG 2844
Pik2 cds Kanto51 CCAGCTGAAGGATTCAAGAGTCTTAAGCTGCTTCGCTTCTTTGCACCTTTAGTGCCGAAG 2844
Pikm2 cds Tsuyuake CCAGCTGAAGGATTCAAGAGTCTTAAGCTGCTTCGCTTCTTTGCACCTTTAGTGCCGAAG 2844
************************************************************
Pi-h1_t_-2 cDNA HR4 CTCAGCTTTTTGGACAAGAATGCAATGCCAGCACTCGAAATCATTGAAATGCGGTTTAAA 2940
Pikp-2 cds K60 CTCAGCTTTTTGGACAAGAATGCAATGCCAGCACTCGAAATCATTGAAATGCGGTTTAAA 2904
Pikh-2 cds K3 CTCAGCTTTTTGGACAAGAATGCAATGCCAGCACTCGAAATCATTGAAATGCGGTTTAAA 2904
Pik2 cds Kanto51 CTCAGCTTTTTGGACAAGAATGCAATGCCAGCACTCGAAATCATTGAAATGCGGTTTAAA 2904
Pikm2 cds Tsuyuake CTCAGCTTTTTGGACAAGAATGCAATGCCAGCACTCGAAATCATTGAAATGCGGTTTAAA 2904
************************************************************
Pi-h1_t_-2 cDNA HR4 GACTTCGAAGGTCTATTTGGCATCGAAATCCTTGAAAATCTCCGTGAGGTGCATCTCAAA 3000
Pikp-2 cds K60 GACTTCGAAGGTCTATTTGGCATCGAAATCCTTGAAAATCTCCGTGAGGTGCATCTCAAA 2964
Pikh-2 cds K3 GACTTCGAAGGTCTATTTGGCATCGAAATCCTTGAAAATCTCCGTGAGGTGCATCTCAAA 2964
Pik2 cds Kanto51 GACTTCGAAGGTCTATTTGGCATCGAAATCCTTGAAAATCTCCGTGAGGTGCATCTCAAA 2964
Pikm2 cds Tsuyuake GACTTCGAAGGTCTATTTGGCATCGAAATCCTTGAAAATCTCCGTGAGGTGCATCTCAAA 2964
************************************************************
Pi-h1_t_-2 cDNA HR4 GTTAGTGATGGGGCAGAAGCAATAACCAAGTTCCTTGTAAATGATTTGAAGGTTAATACT 3060
Pikp-2 cds K60 GTTAGTGATGGGGCAGAAGCAATAACCAAGTTCCTTGTAAATGATTTGAAGGTTAATACT 3024
Pikh-2 cds K3 GTTAGTGATGGGGCAGAAGCAATAACCAAGTTCCTTGTAAATGATTTGAAGGTTAATACT 3024
Pik2 cds Kanto51 GTTAGTGATGGGGCAGAAGCAATAACCAAGTTCCTTGTAAATGATTTGAAGGTTAATACT 3024
Pikm2 cds Tsuyuake GTTAGTGATGGGGCAGAAGCAATAACCAAGTTCCTTGTAAATGATTTGAAGGTTAATACT 3024
************************************************************
Pi-h1_t_-2 cDNA HR4 GAGAAACCAAAAGTATTTGTTGATGGCATCGTCACTGCATGAGAAGTAAAATTGCTGCAA 3120
Pikp-2 cds K60 GAGAAACCAAAAGTATTTGTTGATGGCATCGTCACTGCATGA------3066
Pikh-2 cds K3 GAGAAACCAAAAGTATTTGTTGATGGCATCGTCACTGCATGA------3066
Pik2 cds Kanto51 GAGAAACCAAAAGTATTTGTTGATGGCATCGTCACTGCATGA------3066
Pikm2 cds Tsuyuake GAGAAACCAAAAGTATTTGTTGATGGCATCGTCACTGCATGA------3066
******************************************
Pi-h1_t_-2 cDNA HR4 ATCGGAGAACTTACCAATCATCTGAGGC 3148
Pikp-2 cds K60 ------3066
Pikh-2 cds K3 ------3066
Pik2 cds Kanto51 ------3066
Pikm2 cds Tsuyuake ------3066
Supplementary Fig. B Alignment results of the cDNA sequence of Pi-h1(t)-2 from HR4 between the coding sequences (cds) of Pikp-2 from K60, Pikh-2 from K3, Pik2 from Kanto51 and Pikm2 from Tsuyuake using ClustalW2 multiple sequence alignment tool ( Specific markers amplifying the cDNA sequence from the RNAs of HR4 was developed according to the DNA sequence in shadow background. The coding sequences (cds) of Pikp-2 from K60, Pikh-2 from K3, Pik2 from Kanto51 and Pikm2 from Tsuyuake were obtained from NCBI.
Supplementary Tab. A Scores table of alignment results between the five equivalent sequences on Pik1 allele.Supplementary Tab. B Scores table of alignment results between the five equivalent sequences on Pik2 allele.
Pita-cDNA-HR4 ctaacgaggcataatctcgatcactagctctgatctgatcttcagctagcgccggcgagc60
Pita-cds-Katy ------0
Pita-cds-Yashiro ------0
Pita-cDNA-HR4 tgcagaggtctccatccatggcgccggcggtcattgcatcgcagggtgtcatcatgcggt120
Pita-cds-Katy ------atggcgccggcggtcattgcatcgcagggtgtcatcatgcggt43
Pita-cds-Yashiro ------atggcgccggcggtcattgcatcgcagggtgtcatcatgcggt43
*******************************************
Pita-cDNA-HR4 ccctgacgagcaagctcgactcgctgctgctgcagccgccggagccgccgccgcctgcgc180
Pita-cds-Katy ccctgacgagcaagctcgactcgctgctgctgcagccgccggagccgccgccgcctgcgc103
Pita-cds-Yashiro ccctgacgagcaagctcgactcgctgctgctgcagccgccggagccgccgccgcctgcgc103
************************************************************
Pita-cDNA-HR4 aaccgtcgtcgctgcggaagggggagaggaagaagatcctcctcctcagaggcgatctcc240
Pita-cds-Katy aaccgtcgtcgctgcggaagggggagaggaagaagatcctcctcctcagaggcgatctcc163
Pita-cds-Yashiro aaccgtcgtcgctgcggaagggggagaggaagaagatcctcctcctcagaggcgatctcc163
************************************************************
Pita-cDNA-HR4 gacacctgctagatgactactacctcctcgtggagccgccgtcagacaccgcgccaccgc300
Pita-cds-Katy gacacctgctagatgactactacctcctcgtggagccgccgtcagacaccgcgccaccgc223
Pita-cds-Yashiro gacacctgctagatgactactacctcctcgtggagccgccgtcagacaccgcgccaccgc223
************************************************************
Pita-cDNA-HR4 cagactcgacggcggcgtgctgggctaaggaggttcgcgagctctcctacgacgtcgacg360
Pita-cds-Katy cagactcgacggcggcgtgctgggctaaggaggttcgcgagctctcctacgacgtcgacg283
Pita-cds-Yashiro cagactcgacggcggcgtgctgggctaaggaggttcgcgagctctcctacgacgtcgacg283
************************************************************
Pita-cDNA-HR4 acttcctcgacgagctaacgacccagctcctccaccaccgcggcggcggcgatggcagta420
Pita-cds-Katy acttcctcgacgagctaacgacccagctcctccaccaccgcggcggcggcgatggcagta343
Pita-cds-Yashiro acttcctcgacgagctaacgacccagctcctccaccaccgcggcggcggcgatggcagta343
************************************************************
Pita-cDNA-HR4 gcactgctggtgccaagaagatgatcagcagcatgatcgcgcggcttcgaggggagctta480
Pita-cds-Katy gcactgctggtgccaagaagatgatcagcagcatgatcgcgcggcttcgaggggagctta403
Pita-cds-Yashiro gcactgctggtgccaagaagatgatcagcagcatgatcgcgcggcttcgaggggagctta403
************************************************************
Pita-cDNA-HR4 accggcggcggtggatcgccgacgaggtcaccctgttcagggcccgcgtgaaggaggcca540
Pita-cds-Katy accggcggcggtggatcgccgacgaggtcaccctgttcagggcccgcgtgaaggaggcca463
Pita-cds-Yashiro accggcggcggtggatcgccgacgaggtcaccctgttcagggcccgcgtgaaggaggcca463
************************************************************
Pita-cDNA-HR4 ttcgccgccacgagagctaccatcttggcaggcgcacctcgagctcgaggccgagagaag600
Pita-cds-Katy ttcgccgccacgagagctaccatcttggcaggcgcacctcgagctcgaggccgagagaag523
Pita-cds-Yashiro ttcgccgccacgagagctaccatcttggcaggcgcacctcgagctcgaggccgagagaag523
************************************************************
Pita-cDNA-HR4 aagacgacgacgacgatcgcgaggactccgccggcaacgaacgccgccggtttctgtcgc660
Pita-cds-Katy aagacgacgacgacgatcgcgaggactccgccggcaacgaacgccgccggtttctgtcgc583
Pita-cds-Yashiro aagacgacgacgacgatcgcgaggactccgccggcaacgaacgccgccggtttctgtcgc583
************************************************************
Pita-cDNA-HR4 tgacgttcgggatggacgacgctgctgtgcacggccagctcgttggtagggatatttcga720
Pita-cds-Katy tgacgttcgggatggacgacgctgctgtgcacggccagctcgttggtagggatatttcga643
Pita-cds-Yashiro tgacgttcgggatggacgacgctgctgtgcacggccagctcgttggtagggatatttcga643
************************************************************
Pita-cDNA-HR4 tgcaaaagctcgtccggtggctggccgacggcgagccgaagctcaaggtggcttccattg780
Pita-cds-Katy tgcaaaagctcgtccggtggctggccgacggcgagccgaagctcaaggtggcttccattg703
Pita-cds-Yashiro tgcaaaagctcgtccggtggctggccgacggcgagccgaagctcaaggtggcttccattg703
************************************************************
Pita-cDNA-HR4 ttggatccggaggtgttggcaagacgacgctggccacagaattctatcgtctgcatggcc840
Pita-cds-Katy ttggatccggaggtgttggcaagacgacgctggccacagaattctatcgtctgcatggcc763
Pita-cds-Yashiro ttggatccggaggtgttggcaagacgacgctggccacagaattctatcgtctgcatggcc763
************************************************************
Pita-cDNA-HR4 ggcggttggatgcgccgttcgactgccgggctttcgtgcggacgccccggaagcctgaca900
Pita-cds-Katy ggcggttggatgcgccgttcgactgccgggctttcgtgcggacgccccggaagcctgaca823
Pita-cds-Yashiro ggcggttggatgcgccgttcgactgccgggctttcgtgcggacgccccggaagcctgaca823
************************************************************
Pita-cDNA-HR4 tgacgaagatcctcaccgacatgctgtcacagctgcggccacaacatcagcatcagtctt960
Pita-cds-Katy tgacgaagatcctcaccgacatgctgtcacagctgcggccacaacatcagcatcagtctt883
Pita-cds-Yashiro tgacgaagatcctcaccgacatgctgtcacagctgcggccacaacatcagcatcagtctt883
************************************************************
Pita-cDNA-HR4 cggatgtttgggaggttgatcgactccttgaaactatccggacgcatttgcaagataaaa1020
Pita-cds-Katy cggatgtttgggaggttgatcgactccttgaaactatccggacgcatttgcaagataaaa943
Pita-cds-Yashiro cggatgtttgggaggttgatcgactccttgaaactatccggacgcatttgcaagataaaa943
************************************************************
Pita-cDNA-HR4 ggtacttcatcataattgaagatttatgggcttcatcaatgtgggatattgttagccgtg1080
Pita-cds-Katy ggtacttcatcataattgaagatttatgggcttcatcaatgtgggatattgttagccgtg1003
Pita-cds-Yashiro ggtacttcatcataattgaagatttatgggcttcatcaatgtgggatattgttagccgtg1003
************************************************************
Pita-cDNA-HR4 gtttgcctgataataatagttgcagtagaatactaataacaacagaaattgaacctgtag1140
Pita-cds-Katy gtttgcctgataataatagttgcagtagaatactaataacaacagaaattgaacctgtag1063
Pita-cds-Yashiro gtttgcctgataataatagttgcagtagaatactaataacaacagaaattgaacctgtag1063
************************************************************
Pita-cDNA-HR4 ctttggcatgctgtggatataactcagagcacattattaagattgatccactgggtgatg1200
Pita-cds-Katy ctttggcatgctgtggatataactcagagcacattattaagattgatccactgggtgatg1123
Pita-cds-Yashiro ctttggcatgctgtggatataactcagagcacattattaagattgatccactgggtgatg1123
************************************************************
Pita-cDNA-HR4 atgtctcaagtcaattgtttttcagtggagttgttggccaaggaaatgaatttcctggac1260
Pita-cds-Katy atgtctcaagtcaattgtttttcagtggagttgttggccaaggaaatgaatttcctggac1183
Pita-cds-Yashiro atgtctcaagtcaattgtttttcagtggagttgttggccaaggaaatgaatttcctggac1183
************************************************************
Pita-cDNA-HR4 atcttactgaagtttctcatgacatgataaaaaaatgtggtggcttgccactagcaataa1320
Pita-cds-Katy atcttactgaagtttctcatgacatgataaaaaaatgtggtggcttgccactagcaataa1243
Pita-cds-Yashiro atcttactgaagtttctcatgacatgataaaaaaatgtggtggcttgccactagcaataa1243
************************************************************
Pita-cDNA-HR4 ctataacagccagacattttaaaagccagctgttagatggaatgcagcaatggaatcaca1380
Pita-cds-Katy ctataacagccagacattttaaaagccagctgttagatggaatgcagcaatggaatcaca1303
Pita-cds-Yashiro ctataacagccagacattttaaaagccagctgttagatggaatgcagcaatggaatcaca1303
************************************************************
Pita-cDNA-HR4 tacaaaaatcattgactacttccaatttgaagaaaaatcctactttgcaggggatgaggc1440
Pita-cds-Katy tacaaaaatcattgactacttccaatttgaagaaaaatcctactttgcaggggatgaggc1363
Pita-cds-Yashiro tacaaaaatcattgactacttccaatttgaagaaaaatcctactttgcaggggatgaggc1363
************************************************************
Pita-cDNA-HR4 aagtactcaaccttatttacaataatcttcctcattgtttgaaagcatgtctgttatacc1500
Pita-cds-Katy aagtactcaaccttatttacaataatcttcctcattgtttgaaagcatgtctgttatacc1423
Pita-cds-Yashiro aagtactcaaccttatttacaataatcttcctcattgtttgaaagcatgtctgttatacc1423
************************************************************
Pita-cDNA-HR4 ttagcatctacaaagaggactacataattaggaaggccaacttggtgaggcaatggatgg1560
Pita-cds-Katy ttagcatctacaaagaggactacataattaggaaggccaacttggtgaggcaatggatgg1483
Pita-cds-Yashiro ttagcatctacaaagaggactacataattaggaaggccaacttggtgaggcaatggatgg1483
************************************************************
Pita-cDNA-HR4 ctgaaggtttcatcaattccatagaaaataaagtcatggaagaagttgcagggaactatt1620
Pita-cds-Katy ctgaaggtttcatcaattccatagaaaataaagtcatggaagaagttgcagggaactatt1543
Pita-cds-Yashiro ctgaaggtttcatcaattccatagaaaataaagtcatggaagaagttgcagggaactatt1543
************************************************************
Pita-cDNA-HR4 ttgatgaacttgttggtaggggcctggtccaaccagtagatgttaactgcaaaaatgagg1680
Pita-cds-Katy ttgatgaacttgttggtaggggcctggtccaaccagtagatgttaactgcaaaaatgagg1603
Pita-cds-Yashiro ttgatgaacttgttggtaggggcctggtccaaccagtagatgttaactgcaaaaatgagg1603
************************************************************
Pita-cDNA-HR4 tattgtcatgtgtagtgcaccacatggtattaaatttcatcaggtgtaagtcaatagagg1740
Pita-cds-Katy tattgtcatgtgtagtgcaccacatggtattaaatttcatcaggtgtaagtcaatagagg1663
Pita-cds-Yashiro tattgtcatgtgtagtgcaccacatggtattaaatttcatcaggtgtaagtcaatagagg1663
************************************************************
Pita-cDNA-HR4 agaatttcagcattacattggatcattctcagacgacagtaagacatgctgacaaggttc1800
Pita-cds-Katy agaatttcagcattacattggatcattctcagacgacagtaagacatgctgacaaggttc1723
Pita-cds-Yashiro agaatttcagcattacattggatcattctcagacgacagtaagacatgctgacaaggttc1723
************************************************************
Pita-cDNA-HR4 gccgactctcgcttcacttcagcaatgcacatgatacaacaccactagcaggtttgagac1860
Pita-cds-Katy gccgactctcgcttcacttcagcaatgcacatgatacaacaccactagcaggtttgagac1783
Pita-cds-Yashiro gccgactctcgcttcacttcagcaatgcacatgatacaacaccactagcaggtttgagac1783
************************************************************
Pita-cDNA-HR4 tctcacaagttcgatcgatggcatttttcggacaagtcaagtgtatgccttccattgcag1920
Pita-cds-Katy tctcacaagttcgatcgatggcatttttcggacaagtcaagtgtatgccttccattgcag1843
Pita-cds-Yashiro tctcacaagttcgatcgatggcatttttcggacaagtcaagtgtatgccttccattgcag1843
************************************************************
Pita-cDNA-HR4 attataggcttcttcgagttctgattctttgtttttgggctgatcaagagaaaacaagct1980
Pita-cds-Katy attataggcttcttcgagttctgattctttgtttttgggctgatcaagagaaaacaagct1903
Pita-cds-Yashiro attataggcttcttcgagttctgattctttgtttttgggctgatcaagagaaaacaagct1903
************************************************************
Pita-cDNA-HR4 atgacctcacaagcatttctgaactgttacaactgagatatctgaagataacaggtaata2040
Pita-cds-Katy atgacctcacaagcatttctgaactgttacaactgagatatctgaagataacaggtaata1963
Pita-cds-Yashiro atgacctcacaagcatttctgaactgttacaactgagatatctgaagataacaggtaata1963
************************************************************
Pita-cDNA-HR4 tcacagttaaacttccagagaagatccaaggactacaacacttgcagacactggaagcag2100
Pita-cds-Katy tcacagttaaacttccagagaagatccaaggactacaacacttgcagacactggaagcag2023
Pita-cds-Yashiro tcacagttaaacttccagagaagatccaaggactacaacacttgcagacactggaagcag2023
************************************************************
Pita-cDNA-HR4 atgcaagagcaactgctgtcctattggatattgttcatacacagtgtttgttgcaccttc2160
Pita-cds-Katy atgcaagagcaactgctgtcctattggatattgttcatacacagtgtttgttgcaccttc2083
Pita-cds-Yashiro atgcaagagcaactgctgtcctattggatattgttcatacacagtgtttgttgcaccttc2083
************************************************************
Pita-cDNA-HR4 gtcttgtactacttgatctgctccctcactgtcacaggtacatcttcaccagcatcccca2220
Pita-cds-Katy gtcttgtactacttgatctgctccctcactgtcacaggtacatcttcaccagcatcccca2143
Pita-cds-Yashiro gtcttgtactacttgatctgctccctcactgtcacaggtacatcttcaccagcatcccca2143
************************************************************
Pita-cDNA-HR4 aatggactggaaagctcaacaatctccgcattttaaacattgcagtcatgcaaatttccc2280
Pita-cds-Katy aatggactggaaagctcaacaatctccgcattttaaacattgcagtcatgcaaatttccc2203
Pita-cds-Yashiro aatggactggaaagctcaacaatctccgcattttaaacattgcagtcatgcaaatttccc2203
************************************************************
Pita-cDNA-HR4 aggatgaccttgacactctcaaaggactgggatctctcactgctctttcgctgcttgttc2340
Pita-cds-Katy aggatgaccttgacactctcaaaggactgggatctctcactgctctttcgctgcttgttc2263
Pita-cds-Yashiro aggatgaccttgacactctcaaaggactgggatctctcactgctctttcgctgcttgttc2263
************************************************************
Pita-cDNA-HR4 gaacagcgcctgcgcaaagaatcgtcgctgcgaatgaggggttcgggtctctcaagtact2400
Pita-cds-Katy gaacagcgcctgcgcaaagaatcgtcgctgcgaatgaggggttcgggtctctcaagtact2323
Pita-cds-Yashiro gaacagcgcctgcgcaaagaatcgtcgctgcgaatgaggggttcgggtctctcaagtact2323
************************************************************
Pita-cDNA-HR4 tcatgtttgtctgtacagcaccatgcatgacttttgtggaaggagcaatgccgagtgtgc2460
Pita-cds-Katy tcatgtttgtctgtacagcaccatgcatgacttttgtggaaggagcaatgccgagtgtgc2383
Pita-cds-Yashiro tcatgtttgtctgtacagcaccatgcatgacttttgtggaaggagcaatgccgagtgtgc2383
************************************************************
Pita-cDNA-HR4 aaaggttaaatctaaggttcaatgccaacgagttcaagcagtatgactctaaggagacag2520
Pita-cds-Katy aaaggttaaatctaaggttcaatgccaacgagttcaagcagtatgactctaaggagacag2443
Pita-cds-Yashiro aaaggttaaatctaaggttcaatgccaacgagttcaagcagtatgactctaaggagacag2443
************************************************************
Pita-cDNA-HR4 ggttggaacacttggtcgcccttgcagagatctctgcaagaattgggggcactgatgatg2580
Pita-cds-Katy ggttggaacacttggtcgcccttgcagagatctctgcaagaattgggggcactgatgatg2503
Pita-cds-Yashiro ggttggaacacttggtcgcccttgcagagatctctgcaagaattgggggcactgatgatg2503
************************************************************
Pita-cDNA-HR4 atgaatcaaacaaaactgaagtggagtctgccttgaggactgcaattcgcaagcatccga2640
Pita-cds-Katy atgaatcaaacaaaactgaagtggagtctgccttgaggactgcaattcgcaagcatccga2563
Pita-cds-Yashiro atgaatcaaacaaaactgaagtggagtctgccttgaggactgcaattcgcaagcatccga2563
************************************************************
Pita-cDNA-HR4 cgccgagcactcttatggttgatatacaatgggtggattggatctttggtgctgaaggga2700
Pita-cds-Katy cgccgagcactcttatggttgatatacaatgggtggattggatctttggtgctgaaggga2623
Pita-cds-Yashiro cgccgagcactcttatggttgatatacaatgggtggattggatctttggtgctgaaggga2623
************************************************************
Pita-cDNA-HR4 gagacttggatgaagatttggcacaacaagatgatcacgggttttgtatgctaccggaat2760
Pita-cds-Katy gagacttggatgaagatttggcacaacaagatgatcacgggttttgtatgctaccggaat2683
Pita-cds-Yashiro gagacttggatgaagatttggcacaacaagatgatcacgggtatggatttttcattctat2683
****************************************** * * * * **
Pita-cDNA-HR4 cctcttcacgtcttcagagtagagagataagtatcgaagaagacatgcccaccggtgcgg2820
Pita-cds-Katy cctcttcacgtcttcagagtagagagataagtatcgaagaagacatgcccaccggtgcgg2743
Pita-cds-Yashiro tcccaggttacaacttacaaggattattgagcttctt------tctttctctgccg2733
* * ** * ** ** * * *** *
Pita-cDNA-HR4 aagggacggtgattgtgccccacacaatggagcagttcgccctccacatgagccaagcca2880
Pita-cds-Katy aagggacggtgattgtgccccacacaatggagcagttcgccctccacatgagccaagcca2803
Pita-cds-Yashiro tggcttctatctttacctgct-----atgcat---cttcaacctgacttgatgattgttt2785
* * * ** * *** * * * ** *** *
Pita-cDNA-HR4 agcaatcccacaagctcgtagtcatccagttcactacttcccggtgccctgcttcacgct2940
Pita-cds-Katy agcaatcccacaagctcgtagtcatccagttcactacttcccggtgccctgcttcacgct2863
Pita-cds-Yashiro ga------2787
Pita-cDNA-HR4 acatcgcgccagctttcactgagtatgcgaaggagttcgctggtgcggttttcattaagg3000
Pita-cds-Katy acatcgcgccagctttcactgagtatgcgaaggagttcgctggtgcggttttcattaagg2923
Pita-cds-Yashiro ------2787
Pita-cDNA-HR4 tcaatgtcgacagcgatgagctggagagtgttacggactggtatgacatcgaagggatag3060
Pita-cds-Katy tcaatgtcgacagcgatgagctggagagtgttacggactggtatgacatcgaagggatag2983
Pita-cds-Yashiro ------2787
Pita-cDNA-HR4 tgccaacatttttcttcgtcaaggatggggagaagattgacaagattcctggtgctaaca3120
Pita-cds-Katy tgccaacatttttcttcgtcaaggatggggagaagattgacaagattcctggtgctaaca3043
Pita-cds-Yashiro ------2787
Pita-cDNA-HR4 aggagttgctccgagccaaaattcgaaggcacacagcttctccttacttcctccgttgat3180
Pita-cds-Katy aggagttgctccgagccaaaattcgaaggcacacagcttctccttacttcctccgttga-3102
Pita-cds-Yashiro ------2787
Pita-cDNA-HR4 aatgacttcttttatcatctttgtctagtatatatatggcacttatatagttaactagga3240
Pita-cds-Katy ------3102
Pita-cds-Yashiro ------2787
Pita-cDNA-HR4 agggcaccttgt3252
Pita-cds-Katy ------3102
Pita-cds-Yashiro ------2787
Supplementary Fig. C Alignment results of the cDNA sequence of HR4 between the coding sequences (cds) of Pita from Katy and Yashiro Mochi using ClustalW2 multiple sequence alignment tool ( Specific markers amplifying the cDNA sequence from the RNAs of HR4 was developed according to the DNA sequence in shadow background. The coding sequences (cds) of Pita from Katy and Yashiro Mochi were obtained from NCBI. ‘Yashiro’ in the figure is the abbreviation of ‘Yashiro Mochi’.
Supplementary Tab. C Scores table of alignment results between the three sequences
Supplementary Tab. D Resistance reactions of HR4, R170 and its two improved lines R1701 and R1702 against forty diverse isolates
Isolates / R1701 / R1702 / HR4 / R170 / Isolates / R1701 / R1702 / HR4 / R170 / Isolates / R1701 / R1702 / HR4 / R170GD0016 / R / R / R / R / GD07119 / R / R / R / S / GD08T6 / S / S / R / S
GD00120 / S / S / R / S / GD07127 / R / R / R / R / GD08T28 / S / S / R / S
GD00193 / R / R / R / S / GD07153 / R / R / R / S / GD08T29 / S / S / R / S
GD0118 / R / R / R / R / GD07164 / R / R / R / R / GD09167 / R / R / R / S
GD0166 / R / R / R / S / GD08472 / R / R / R / R / GD09318 / R / R / R / R
GD01154 / S / S / S / S / GD08507 / R / R / R / R / GD93203 / R / R / R / S
GD01165 / R / R / R / S / GD08682 / R / R / R / S / GD93286 / R / R / R / S
GD0525 / R / R / R / S / GD08597 / R / R / R / S / GD9559 / R / R / R / S
GD05135 / R / R / R / R / GD08679 / R / R / R / S / GD97322 / R / R / R / S
GD05141 / R / R / R / S / GD08712 / R / R / R / R / GD9866 / R / R / R / S
GD05166 / R / R / R / R / GD08753 / R / R / R / S / GD98236 / R / R / R / S
GD06105 / R / R / R / S / GD08923 / R / R / R / R / GD98288 / R / R / R / S
GD0732 / R / R / R / R / GD08T3 / S / S / R / S / RS (%) / 82.5 / 82.5 / 100.0 / 32.5
GD07116 / R / R / R / R / GD08T4 / S / S / R / S
R resistant, S susceptible
RS resistance spectrum % = (no. of incompatible isolates/no. of total tested isolates) × 100 %
The two improved lines R1701 and R1702 derived from two individuals of BC3F2 population of R1704/HR4. In the process of improvement, R170 and HR4 were used as the recurrent parent and the donor parent, respectively. We screened each segregating generation, inclduing BC1F1, BC2F1, BC3F1 and BC3F2, with two flanked SSR markers RM224 and RM144. Finally, several individuals carrying homozygous Pi-h1(t) were obtained in the BC3F2 population by MAS, and two individuals which looked similarly with the recurrent parent mostly at the evaluation of a panel of four experienced experts in rice breeding were selected to produce improved lines R1701 and R1702 by self-pollination.