Supplementary Table 1. Correlations between AGE/ 8-OHdH/ IL-6/ adiponectin and oxidative stress-related parameters, calorie as well as protein intake
AGE (ug/ml) / Plasma-8-OHdG (pg/ml) 1 / IL-6 (ug/ml) 1 / Adiponectin (ug/ml)1Vitamin E (μM/mg) / 0.127 (0.435)2 / 0.097 (0.550) / 0.076 (0.570) / -0.127 (0.338)
RBC-TAS (mg Trolox Eqv) / -0.100 (0.540)2 / 0.021 (0.897) / 0.077 (0.560) / -0.168 (0.203)
Plasma-GSH (μM/mg) / 0.060 (0.712)2 / -0.061 (0.707) / -0.022 (0.867) / 0.009 (0.944)
RBC-GSH (μM/mg) / -0.115 (0.481)2 / 0.134 (0.411) / 0.061 (0.644) / 0.147 (0.268)
RBC-GPx (unit/mg protein) / -0.067 (0.681)2 / 0.073 (0.652) / -0.128 (0.334) / -0.041 (0.760)
Plasma-GPx (unit/mg protein) / -0.349 (0.027)2 / 0.040 (0.804) / -0.180 (0.173) / -0.277 (0.034)
RBC-CAT (unit/μg protein) / -0.119 (0.464)2 / 0.203 (0.210) / 0.118 (0.374) / -0.16 (0.225)
Plasma-CAT (unit/μg protein) / 0.022 (0.893)1 / -0.341 (0.032) / 0.059 (0.657) / -0.216 (0.101)
RBC-SOD (unit/mg protein) / 0.047 (0.773)2 / 0.083 (0.610) / 0.023 (0.865) / 0.08 (0.549)
Plasma-SOD (unit/mg protein) / -0.102 (0.531)2 / 0.041 (0.803) / -0.224 (0.088) / -0.382 (0.003)
Plasma-MDA (μM/mg) / -0.005 (0.978)2 / 0.024 (0.883) / 0.247 (0.059) / 0.017 (0.899)
RBC-MDA (μM/mg) / -0.184 (0.255)2 / 0.270 (0.092) / 0.247 (0.059) / 0.03 (0.822)
Calorie intake (Kcal) / -0.396 (0.018) 2 / -0.060 (0.730) / 0.077 (0.593) / -0.197 (0.165)
Protein intake (g) / -0.317 (0.063) 2 / 0.050 (0.747) / -0.007 (0.963) / -0.258 (0.068)
AGE: Advanced glycation end product; OHdG: hydroxy-2-deoxyguanosine; IL: interleukin; RBC: red blood cell; MDA: Malondaldegyde; TAS: total anti-oxidant status; GSH: glutathione; GPx: glutathione peroxidase; CAT: catalase; SOD: superoxide dismutase Correlation coefficients were estimated by Spearman’s rank correlation1 for variables with skewed distribution and others were estimated by Pearson’s correlation2.
Bold letter indicates statistical significance, p < .05.
Supplementary Table 2. Correlations between 8-OHdH/ AGE/ IL-6/ adiponectin and biochemical parameters
eGFR (mls/min/1.73m2) / -0.059 (0.718)2 / -0.588 (<0.001) / -0.217 (0.099) / -0.106 (0.423)
Serum creatinine (mg/dl) / -0.001 (0.993)2 / 0.571 (<0.001) / 0.141 (0.287) / -0.07 (0.596)
uCr (mg/dl) / 0.049 (0.763)2 / -0.032 (0.846) / -0.111 (0.404) / -0.324 (0.012)
Albumin in spot morning urine (mg/dl) / -0.174 (0.283)1 / 0.229 (0.156) / 0.271 (0.057) / 0.068 (0.637)
UACR (mg/g) / -0.088(0.588)1 / 0.548(<0.001) / 0.306 (0.018) / 0.008 (0.952)
Glucose (mg/dl) / 0.123 (0.448)2 / 0.244 (0.130) / 0.347 (0.007) / -0.066 (0.619)
HbA1c (%) / -0.012 (0.942)2 / 0.260 (0.106) / 0.350 (0.007) / -0.199 (0.132)
ALT (µ/l) / -0.265 (0.098)1 / -0.258 (0.107) / <0.001 (0.999) / -0.431 (0.001)
HDL-C (mg/dl) / 0.057 (0.727)2 / -0.146 (0.367) / 0.144 (0.278) / 0.485 (<0.001)
TCH (mg/dl) / 0.28 (0.080)2 / -0.103 (0.527) / 0.013 (0.921) / 0.028 (0.831)
TG (mg/dl) / 0.004 (0.980)1 / 0.335 (0.035) / -0.03 (0.821) / -0.255 (0.051)
hs-CRP (mg/dl) / -0.033 (0.849)1 / 0.092 (0.599) / 0.376 (0.008) / 0.068 (0.644)
AGE: Advanced glycation end product; OHdG: hydroxy-2-deoxyguanosine; IL: interleukin; eGFR: stimated glomerular filtration rate; CKD: chronic kidney disease; ALT: alanine aminotransferase; HDL-C: high density lipoprotein cholesterol; TCH: total cholesterol; TG: triglyceride; uCr: urine creatine; hs-CRP: high-sensitivity C-reactive protein; UACR: urine albumin-to-creatinine ratio
Correlation coefficients were estimated by Spearman’s rank correlation1 for variables with skewed distribution and others were estimated by Pearson’s correlation2.
Bold letter indicates statistical significance, p < .05.
NANANANANA
Supplementary Table 3. Levels of 8-OHdH/ AGE/ IL-6/ adiponectin and biochemical parameters according to factors of health behavior
Smoking / 0.758 / 0.441 / 0.373 / 0.123
No / 3.9 ± 0.6 / 609.0 (557.3, 663.8) / 8.3 (7.3, 9.3) / 21.7 (13.3, 38.8)
Yes / 4.2 ± 1.6 / 643.6 (559.9, 821.3) / 7.7 (6.2, 9.1) / 13.4 (9.8, 15.8)
Snake / 0.045 / 0.485 / 0.409 / 0.249
No / 4.0 ± 0.6 / 613.6 (554.7, 663.8) / 8.3 (6.7, 9.7) / 20.1 (15, 43)
Yes / 3.5 ± 0.8 / 609.0 (600.1, 663.8) / 8.4 (8.1, 9.3) / 18.5 (13.3, 25.2)
Sporting habit / 0.279 / 0.587 / 0.873 / 0.295
No / 4.0 ± 0.7 / 612.9 (559.9, 677.9) / 7.8 (7.1, 9.7) / 19.1 (10.5, 28.9)
Yes / 3.7 ± 0.6 / 609.0 (557.3, 651.9) / 8.4 (7, 9.1) / 22.2 (15.4, 41.1)
Vegetarian / 0.965 / 0.829 / 0.615 / 0.315
No / 3.9 ± 0.7 / 609.0 (557.3, 663.8) / 8.3 (7, 9.2) / 19.3 (12.6, 38.8)
Yes / 3.9† / 603.1† / 9.9 (7.1, 12.6) / 14.8 (6.5, 23)
Fruit / 0.144 / 0.255 / 0.923 / 0.165
< 1 serving / 4.2 ± 0.6 / 651.9 (609, 698.1) / 8.5 (7.5, 10) / 39.3 (20.4, 43.9)
1-2 serving(s) / 3.8 ± 0.7 / 607.5 (552.8, 663.8) / 8.4 (7.1, 9.3) / 18.4 (12.6, 41.6)
>2 servings / 3.5 ± 0.6 / 599.4 (562.6, 638.2) / 8.6 (7, 9.7) / 18.5 (13, 23.6)
Health supplements / NA / NA / 0.717 / 0.334
No / 3.9 ± 0.7 / 609 (558.6, 663.8) / 8.4 (7.1, 9.2) / 19.7 (13, 37.1)
Yes / NA / NA / 7.4 (6, 12.6) / 6.5 (1.8, 53.4)
NA= not applicable
AGE are presented as mean ± standard deviation and tested by analysis of variance for fruit intake or independent t-test for others. Plasma-8-OHdG, IL-6, adiponectin are presented as median (interquartile range) and tested by Kruskal-Wallis for fruit intake or Mann-Whitney U test for others.
Bold value indicates significantly different between groups, p < 0.05.
† Of patients having data on AGE and plasma-8-OHdG, there is only one vegetarian.