Online Table 1 Description of Snps Used in This Study

Online Table 1 Description of SNPs used in this study

SNP / Alleles / Amino Acid Substitution / Location
CD14
rs4914 / C/G / Synonymous / Exon 1
TLR2
rs1816702 / C/T / Intron 2
rs1898830 / A/G / Intron 1
rs3804099 / C/T / Synonymous / Exon 3
rs3804100 / C/T / Synonymous / Exon 3
rs11938228 / A/C / Intron 2
TLR4
rs1927907 / A/G / Intron 2
rs1927911 / C/T / Intron 1
rs4986790 / A/G / Gly → Asp / Exon 3
rs4986791 / C/T / Ile → Thr / Exon 3
rs5030717 / A/G / Intron 2
rs12344353 / C/T / Intron 1
rs5030718 / A/G / Lys → Glu / Exon 3
rs5030719 / G/T / His → Gln / Exon 3
rs5030728 / A/G / Intron 2
rs11536869 / A/G / Intron 1
MPO
rs2071409 / A/C / Intron 11
rs7208693 / A/C / Phe → Val / Exon 2
rs8067377 / C/T / Intron 8
rs28730837 / C/T / Val → Ala / Exon 6

Online Table 2 Weighted means of percent positive cells (%) and median fluorescence intensity (MFI) by CD14 rs4914 for blacks (B) and whites (W)

Monocyte Markers / Unit / Race / CC / CG
or *CG/GG / GG / P-value
CD14+/TLR4+ / % / B / 64.1 ± 0.21 / 64.0 ± 0.47 / --- / 0.80
W / 65.4 ± 0.17 / 65.4 ± 0.30 / 64.7 ± 0.97 / 0.76
MFI / B / 16.7 ± 0.06 / 16.6 ± 0.12 / --- / 0.57
W / 16.4 ± 0.04 / 16.4 ± 0.07 / 16.3 ± 0.20 / 0.73
CD14+/CD162+ / MFI / B / 114.7 ± 0.86 / 113.6 ± 2.15 / --- / 0.64
W / 112.1 ± 0.57 / 109.4 ± 1.28 / 113.8 ± 3.80 / 0.13
CD14+ / MFI / B / 120.4 ± 1.31 / 120.2 ± 3.52 / --- / 0.94
W / 108.9 ± 0.77 / 111.4 ± 1.57 / 118.0 ± 5.00 / 0.08
CD14+/TLR2+ / % / B / 55.8 ± 0.63 / 56.1 ± 1.63 / --- / 0.86
W / 64.1 ± 0.43 / 63.1 ± 0.90 / 61.7 ± 4.91 / 0.53
MFI / B / 13.5 ± 0.04 / 13.4 ± 0.10 / --- / 0.88
W / 14.1 ± 0.06 / 14.0 ± 0.10 / 13.9 ± 0.35 / 0.82

*CT and TT genotypes were collapsed in blacks.

Online Table 3 Weighted means of percent positive cells (%) and median fluorescence intensity (MFI) by TLR2 rs1816702 for blacks (B) and whites (W)

Monocyte Markers / Unit / Race / CC / CT
or *CT/TT / TT / P-value
CD14+/TLR2+/CD162+ / % / B / 57.3 ± 1.0 / 56.8 ± 0.81 / 59.1 ± 1.63 / 0.45
W / 64.3 ± 0.42 / 68.7 ± 0.97 / --- / < 0.001
MFI / B / 13.3 ± 0.08 / 13.3 ± 0.05 / 13.4 ± 0.10 / 0.56
W / 13.8 ± 0.04 / 14.4 ± 0.23 / --- / 0.01
CD14+/TLR2+ / % / B / 55.7 ± 0.99 / 55.3 ± 0.79 / 57.6 ± 1.64 / 0.44
W / 63.0 ± 0.42 / 67.4 ± 0.98 / --- / < 0.001
MFI / B / 13.4 ± 0.08 / 13.4 ± 0.05 / 13.5 ± 0.09 / 0.65
W / 14.0 ± 0.04 / 14.6 ± 0.22 / --- / 0.01

*CT and TT genotypes were collapsed in whites.

Online Table 4 Weighted means of percent positive cells (%) and median fluorescence intensity (MFI) by TLR2 rs1898830 for blacks (B) and whites (W)

Monocyte Markers / Unit / Race / AA / AG
or *AG/GG / GG / P-value
CD14+/TLR2+/CD162+ / % / B / 57.1 ± 0.67 / 58.1 ± 1.23 / --- / 0.46
W / 65.5 ± 0.65 / 65.0 ± 0.55 / 65.1 ± 1.13 / 0.82
MFI / B / 13.3 ± 0.05 / 13.4 ± 0.09 / --- / 0.46
W / 14.1 ± 0.12 / 13.9 ± 0.05 / 13.9 ± 0.12 / 0.25
CD14+/TLR2+ / % / B / 55.6 ± 0.67 / 56.5 ± 1.19 / --- / 0.52
W / 64.3 ± 0.64 / 63.5 ± 0.55 / 63.8 ± 1.13 / 0.64
MFI / B / 13.4 ± 0.05 / 13.5 ± 0.08 / --- / 0.55
W / 14.2 ± 0.12 / 14.0 ± 0.05 / 14.1 ± 0.11 / 0.16

*Genotypes were collapsed in blacks.

Online Table 5 Weighted means of percent positive cells (%) and median fluorescence intensity (MFI) by TLR2 rs3804099 for blacks (B) and whites (W)

Monocyte Markers / Unit / Race / TT / CT / CC / P-value
CD14+/TLR2+/CD162+ / % / B / 58.5 ± 1.15 / 57.6 ± 0.89 / 56.6 ± 1.00 / 0.45
W / 64.3 ± 0.64 / 65.5 ± 0.57 / 65.9 ± 0.98 / 0.25
MFI / B / 13.3 ± 0.08 / 13.3 ± 0.06 / 13.3 ± 0.07 / 0.91
W / 13.8 ± 0.06 / 14.0 ± 0.10 / 14.0 ± 0.08 / 0.05
CD14+/TLR2+ / % / B / 56.9 ± 1.13 / 56.1 ± 0.88 / 55.1 ± 1.00 / 0.51
W / 62.9 ± 0.64 / 64.0 ± 0.58 / 64.8 ± 1.00 / 0.20
MFI / B / 13.4 ± 0.08 / 13.5 ± 0.06 / 13.4 ± 0.07 / 0.83
W / 13.9 ± 0.05 / 14.1 ± 0.10 / 14.2 ± 0.07 / 0.04

Online Table 6 Weighted means of percent positive cells (%) and median fluorescence intensity (MFI) by TLR2 rs3804100 for blacks (B) and whites (W)

Monocyte Markers / Unit / Race / TT / CT
or *CT/CC / CC / P-value
CD14+/TLR2+/CD162+ / % / B / 57.1 ± 0.63 / 58.6 ± 1.66 / --- / 0.42
W / 65.5 ± 0.42 / 63.4 ± 1.12 / 61.7 ± 1.66 / 0.03
MFI / B / 13.3 ± 0.04 / 13.5 ± 0.12 / --- / 0.26
W / 14.0 ± 0.06 / 13.8 ± 0.10 / 13.7 ± 0.12 / 0.04
CD14+/TLR2+ / % / B / 55.6 ± 0.62 / 57.0 ± 1.69 / --- / 0.43
W / 64.1 ± 0.42 / 62.3 ± 1.11 / 60.2 ± 1.63 / 0.03
MFI / B / 13.4 ± 0.04 / 13.6 ± 0.12 / --- / 0.29
W / 14.1 ± 0.06 / 13.9 ± 0.09 / 13.8 ± 0.11 / 0.03

*Genotypes were collapsed in blacks.

Online Table 7 Weighted means of percent positive cells (%) and median fluorescence intensity (MFI) by TLR2 rs11938228 for blacks (B) and whites (W)

Monocyte Markers / Unit / Race / CC / AC
or *AC/AA / AA / P-value
CD14+/TLR2+/CD162+ / % / B / 57.3 ± 0.68 / 57.4 ± 1.20 / --- / 0.95
W / 65.6 ± 0.64 / 64.6 ± 0.57 / 66.2 ± 1.01 / 0.33
MFI / B / 13.3 ± 0.05 / 13.3 ± 0.09 / --- / 0.70
W / 14.1 ± 0.13 / 13.8 ± 0.05 / 14.0 ± 0.11 / 0.09
CD14+/TLR2+ / % / B / 55.8 ± 0.68 / 55.8 ± 1.17 / --- / 0.96
W / 64.4 ± 0.64 / 63.2 ± 0.57 / 64.9 ± 1.01 / 0.23
MFI / B / 13.44 ± 0.05 / 13.5 ± 0.08 / --- / 0.78
W / 14.2 ± 0.12 / 14.0 ± 0.05 / 14.1 ± 0.10 / 0.06

*Genotypes were collapsed in blacks.

Online Table 8 Weighted means of percent positive cells (%) and median fluorescence intensity (MFI) by TLR4 rs1927907 for blacks (B) and whites (W)

Monocyte Markers / Unit / Race / GG / AG / AA / P-value
CD14+/CD41+/TLR4+ / % / B / 11.8 ± 0.19 / 11.7 ± 0.28 / 11.5 ± 0.38 / 0.77
W / 11.1 ± 0.11 / 11.1 ± 0.20 / 11.1 ± 0.76 / 1.00
MFI / B / 48.5 ± 0.50 / 48.6 ± 0.75 / 49.5 ± 1.05 / 0.71
W / 46.2 ± 0.23 / 46.5 ± 0.39 / 47.6 ± 1.40 / 0.52
CD14+/TLR4+ / % / B / 64.0 ± 0.26 / 64.0 ± 0.31 / 64.9 ± 0.61 / 0.34
W / 65.3 ± 0.16 / 65.6 ± 0.32 / 66.1 ± 1.57 / 0.65
MFI / B / 16.6 ± 0.07 / 16.7 ± 0.10 / 16.9 ± 0.18 / 0.36
W / 16.4 ± 0.04 / 16.33 ± 0.08 / 16.2 ± 0.32 / 0.47

Online Table 9 Weighted means of percent positive cells (%) and median fluorescence intensity (MFI) by TLR4 rs1927911 for blacks (B) and whites (W)

Monocyte Markers / Unit / Race / CC / CT / TT / P-value
CD14+/CD41+/TLR4+ / % / B / 11.5 ± 0.41 / 12.0 ± 0.23 / 11.5 ± 0.21 / 0.20
W / 11.0 ± 0.12 / 11.2 ± 0.16 / 11.3 ± 0.35 / 0.46
MFI / B / 48.1 ± 0.81 / 48.2 ± 0.63 / 49.3 ± 0.57 / 0.33
W / 46.2 ± 0.28 / 46.4 ± 0.31 / 47.0 ± 0.71 / 0.57
CD14+/TLR4+ / % / B / 63.9 ± 0.59 / 64.0 ± 0.27 / 64.2 ± 0.30 / 0.83
W / 65.4 ± 0.19 / 65.1 ± 0.22 / 66.6 ± 0.78 / 0.12
MFI / B / 16.6 ± 0.15 / 16.6 ± 0.07 / 16.7 ± 0.09 / 0.64
W / 16.4 ± 0.04 / 16.4 ± 0.06 / 16.4 ± 0.19 / 0.60

Online Table 10 Weighted means of percent positive cells (%) and median fluorescence intensity (MFI) by TLR4 rs4986790 for blacks (B) and whites (W)

Monocyte Markers / Unit / Race / AA / AG/GG / P-value
CD14+/CD41+/TLR4+ / % / B / 11.8 ± 0.16 / 11.1 ± 0.42 / 0.14
W / 11.2 ± 0.10 / 10.7 ± 0.24 / 0.08
MFI / B / 48.6 ± 0.42 / 48.6 ± 1.04 / 0.95
W / 46.3 ± 0.21 / 46.4 ± 0.53 / 0.81
CD14+/TLR4+ / % / B / 64.2 ± 0.21 / 63.7 ± 0.44 / 0.32
W / 65.44 ± 0.16 / 65.1 ± 0.41 / 0.37
MFI / B / 16.7 ± 0.06 / 16.4 ± 0.11 / 0.004
W / 16.4 ± 0.04 / 16.2 ± 0.09 / 0.04

*Genotypes were collapsed in blacks.

Online Table 11 Weighted means of percent positive cells (%) and median fluorescence intensity (MFI) by TLR4 rs4986791 for whites

Monocyte Markers / Unit / Race / CC / CT/TT / P-value
CD14+/CD41+/TLR4+ / % / W / 11.2 ± 0.10 / 10.7 ± 0.27 / 0.16
MFI / W / 46.3 ± 0.21 / 46.2 ± 0.52 / 0.86
CD14+/TLR4+ / % / W / 65.4 ± 0.16 / 65.0 ± 0.40 / 0.33
MFI / W / 16.4 ± 0.04 / 16.2 ± 0.09 / 0.03

Online Table 12 Weighted means of percent positive cells (%) and median fluorescence intensity (MFI) by TLR4 rs5030717 for blacks (B) and whites (W)

Monocyte Markers / Unit / Race / AA / AG / GG / P-value
CD14+/CD41+/TLR4+ / % / B / 11.8 ± 0.18 / 11.5 ± 0.27 / 12.4 ± 0.72 / 0.33
W / 11.1 ± 0.10 / 11.2 ± 0.23 / 11.8 ± 1.12 / 0.74
MFI / B / 48.6 ± 0.45 / 48.8 ± 0.81 / 46.7 ± 2.24 / 0.68
W / 46.3 ± 0.22 / 46.2 ± 0.42 / 48.1 ± 2.24 / 0.72
CD14+/TLR4+ / % / B / 63.8 ± 0.24 / 64.5 ± 0.32 / 65.5 ± 1.00 / 0.09
W / 65.3 ± 0.15 / 65.6 ± 0.37 / 65.5 ± 2.58 / 0.83
MFI / B / 16.6 ± 0.06 / 16.7 ± 0.11 / 17.2 ± 0.31 / 0.17
W / 16.4 ± 0.04 / 16.3 ± 0.09 / 16.1 ± 0.49 / 0.50

Online Table 13 Weighted means of percent positive cells (%) and median fluorescence intensity (MFI) by TLR4 rs12344353 for blacks (B) and whites (W)

Monocyte Markers / Unit / Race / TT / TC/TT / P-value
CD14+/CD41+/TLR4+ / % / B / 11.6 ± 0.17 / 11.9 ± 0.29 / 0.43
W / 11.2 ± 0.10 / 10.6 ± 0.23 / 0.03
MFI / B / 48.7 ± 0.48 / 48.5 ± 0.67 / 0.85
W / 46.3 ± 0.21 / 46.6 ± 0.52 / 0.64
CD14+/TLR4+ / % / B / 64.1 ± 0.22 / 64.0 ± 0.40 / 0.85
W / 65.4 ± 0.16 / 65.2 ± 0.41 / 0.62
MFI / B / 16.7 ± 0.07 / 16.6 ± 0.09 / 0.23
W / 16.4 ± 0.04 / 16.2 ± 0.09 / 0.07

Online Table 14 Weighted means of percent positive cells (%) and median fluorescence intensity (MFI) by TLR4 rs5030718 for blacks

Monocyte Markers / Unit / Race / GG / AG/GG / P-value
CD14+/CD41+/TLR4+ / % / B / 11.7 ± 0.15 / 12.0 ± 0.46 / 0.56
MFI / B / 48.5 ± 0.41 / 50.8 ± 1.38 / 0.11
CD14+/TLR4+ / % / B / 64.1 ± 0.19 / 63.8 ± 0.87 / 0.76
MFI / B / 16.7 ± 0.06 / 16.7 ± 0.22 / 0.84

Online Table 15 Weighted means of percent positive cells (%) and median fluorescence intensity (MFI) by TLR4 rs5030719 for blacks

Monocyte Markers / Unit / Race / GG / GT/TT / P-value
CD14+/CD41+/TLR4+ / % / B / 11.74 ± 0.15 / 11.3 ± 0.51 / 0.44
MFI / B / 48.6 ± 0.40 / 49.2 ± 1.64 / 0.70
CD14+/TLR4+ / % / B / 64.2 ± 0.20 / 63.0 ± 0.56 / 0.05
MFI / B / 16.7 ± 0.05 / 16.0 ± 0.14 / <0.001

Online Table 16 Weighted means of percent positive cells (%) and median fluorescence intensity (MFI) by TLR4 rs5030728 for blacks (B) and whites (W)

Monocyte Markers / Unit / Race / GG / AG
or *AG/AA / AA / P-value
CD14+/CD41+/TLR4+ / % / B / 11.7 ± 0.17 / 11.7 ± 0.31 / --- / 1.00
W / 11.2 ± 0.13 / 10.9 ± 0.14 / 11.4 ± 0.33 / 0.19
MFI / B / 49.2 ± 0.43 / 46.9 ± 0.87 / --- / 0.02
W / 46.1 ± 0.28 / 46.7 ± 0.30 / 45.9 ± 0.72 / 0.35
CD14+/TLR4+ / % / B / 64.0 ± 0.22 / 64.3 ± 0.36 / --- / 0.56
W / 65.4 ± 0.22 / 65.4 ± 0.20 / 65.4 ± 0.55 / 0.99
MFI / B / 16.7 ± 0.06 / 16.7 ± 0.11 / --- / 0.76
W / 16.4 ± 0.05 / 16.4 ± 0.05 / 16.4 ± 0.13 / 0.91

*Genotypes were collapsed in blacks.

Online Table 17 Weighted means of percent positive cells (%) and median fluorescence intensity (MFI) by TLR4 rs11536869 for whites

Monocyte Markers / Unit / Race / AA / AG/GG / P-value
CD14+/CD41+/TLR4+ / % / W / 11.1 ± 0.10 / 10.9 ± 0.31 / 0.53
MFI / W / 46.33 ± 0.21 / 46.2 ± 0.59 / 0.84
CD14+/TLR4+ / % / W / 65.4 ± 0.15 / 65.8 ± 0.66 / 0.55
MFI / W / 16.4 ± 0.04 / 16.4 ± 0.13 / 0.81

Online Table 18 Weighted means of percent positive cells (%) and median fluorescence intensity (MFI) by MPO rs2071409 for blacks (B) and whites (W)

Monocyte Markers / Unit / Race / AA / AC
or *AC/CC / CC / P-value
CD45+/MPO+ / MFI / B / 101.5 ± 1.93 / 104.5 ± 2.99 / --- / 0.40
W / 89.4 ± 0.96 / 90.3 ± 1.48 / 91.5 ± 3.11 / 0.75
CD45+ / MFI / B / 71.0 ± 0.63 / 69.5 ± 1.07 / --- / 0.24
W / 76.5 ± 0.40 / 75.9 ± 0.68 / 72.9 ± 3.02 / 0.40

*Genotypes were collapsed in blacks.

Online Table 19 Weighted means of percent positive cells (%) and median fluorescence intensity (MFI) by MPO rs7208693 for blacks (B) and whites (W)

Monocyte Markers / Unit / Race / CC / AC
or AC/AA / AA / P-value
CD45+/MPO+ / MFI / B / 105.0 ± 2.06 / 96.4 ± 2.70 / 101.9 ± 6.37 / 0.04
W / 90.0 ± 0.89 / 88.0 ± 1.69 / --- / 0.30
CD45+ / MFI / B / 70.2 ± 0.61 / 71.4 ± 1.17 / 70.9 ± 2.11 / 0.67
W / 76.4 ± 0.38 / 75.4 ± 0.88 / --- / 0.28

*Genotypes were collapsed in whites.

Online Table 20 Weighted means of percent positive cells (%) and median fluorescence intensity (MFI) by MPO rs8067377 for blacks

Monocyte Markers / Unit / Race / CC / CT/TT / P-value
CD45+/MPO+ / MFI / B / 104.2 ± 1.78 / 93.0 ± 3.80 / 0.008
CD45+ / MFI / B / 70.3 ± 0.59 / 72.1 ± 1.42 / 0.24

Online Table 21 Weighted means of percent positive cells (%) and median fluorescence intensity (MFI) by MPO rs28730837 for whites

Monocyte Markers / Unit / Race / CC / CT/TT / P-value
CD45+/MPO+ / MFI / W / 90.2 ± 0.80 / 79.9 ± 3.59 / 0.005
CD45+ / MFI / W / 76.2 ± 0.36 / 77.0 ± 1.39 / 0.56

Online Table 22 Weighted means of percent positive cells (%) and median fluorescence intensity (MFI) by TLR4 rs4986790/rs4986791 for blacks (B) and whites (W)

Monocyte Markers / Unit / Race / AA-CC / AG-CC or GG-CC / AG-CT or GG-CT / P-value
CD14+/CD41+/TLR4+ / % / B / 11.8 ± 0.16 / 11.2 ± 0.42 / - / 0.16
W / 11.1 ± 0.10 / - / 10.7 ± 0.24 / 0.08
MFI / B / 48.6 ± 0.43 / 48.4 ± 1.10 / - / 0.85
W / 46.4 ± 0.21 / - / 46.4 ± 0.53 / 0.92
CD14+/TLR4+ / % / B / 64.2 ± 0.21 / 63.7 ± 0.47 / - / 0.39
W / 65.5 ± 0.16 / - / 65.0 ± 0.42 / 0.34
MFI / B / 16.7 ± 0.06 / 16.4 ± 0.12 / - / 0.007
W / 16.4 ± 0.04 / - / 16.2 ± 0.09 / 0.03

Online Table 23 Sample Size for TLR4 rs4986790/rs4986791 genotype combinations

Group / Whites / Blacks
AA-CC / 1180 / 375
AG-CC or GG-CC / 2 / 46
AG-CT or GG-CT / 156 / 8