OIE RL for « Salmonella/Salmonellosis » – « Dr.Rob Davies » – « United Kingdom »

OIE Reference Laboratory Reports
Activities in 2012

Name of disease (or topic) for which you are a designated OIE Reference Laboratory: / Salmonella / Salmonellosis
Address of laboratory / Animal Health and Veterinary Laboratories Agency Weybridge
Woodham Lane, New Haw, Addlestone
SurreyKT15 3NB, UK
Tel.: / (+44) (0) 1932-357 361
Fax: / (+44) (0) 1932-357 595
e-mail address: /
website: /
Name (including Title) of Head of Laboratory (Responsible Official): / Prof. Glyn Hewinson
Name (including Title and Position) of OIE Reference Expert: / Dr. Robert H. Davies
Date of submission to the OIE / 25th of January 2013

Instructions

This form should be used by an OIE Reference Laboratory to report activities that took place from January through December of the past year (2012), unless otherwise stated, and must be submitted by the end of January every year.

Only those activities that concern the disease (or topic) for which the laboratory is recognised by the OIE should be mentioned. The questionnaire structure follows the Terms of Reference (ToRs) for OIE Reference Laboratories, available at:

Each ToR(blue italicised text)has been placed as a heading covering the group of questions related to it.

Please note the red italicised text is given as guidance and should be deleted from your report and substitute with your data. Examples are based on past Annual Reports or have been invented.

The questionnaire represents a means of gathering information on activities carried out by OIE Reference Laboratories and making it available to OIE Member Countries and to the OIE Reference Laboratory network.

This annual report will remain available for consultation on the OIE web site:

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ToR:To use, promote and disseminate diagnostic methods validated according to OIE Standards

Test recommended by the OIE / Total number of test performed last year
Indirect diagnostic tests / Nationally / Internationally
SAT (S.Gallinarum/Pullorum) / 142
SAT (S.Abortusequi) / 187 / 3
SAT (S. Dublin) / 75
SAT (S.Typhimurium) / 63
Direct diagnostic tests / Nationally / Internationally

Serotyping

/ 7,705 / 27

Phagetyping

/ 935 / 1

Antimicrobial Susceptibility Testing

/ 3,748 / 18

Tests for live Salmonella vaccines

/ 167

Salmonella culture

/ 13,479

PCR (S. Typhimurium confirmation)

/ 41

PCR (thrW island)

/ 91

PCR (duplex) (fljB and intergenic region)

/ 41

Genome sequencing (S. 4,[5],12:i:- and S. Typhimurium)

/ 220

VNTR (S. Typhimurium)

/ 70

ToR:To develop reference material in accordance with OIE requirements, and implement and promote the application of OIE Standards.

To store and distribute to national laboratories biological reference products and any other reagents used in the diagnosis and control of the designated pathogens or disease.

2.Did your laboratory produce or store imported standard reference reagents officially recognised by the OIE or other international bodies?

Yes No

3.Did your laboratory supply standard reference reagents to OIE Member Countries?

Yes No

Type of reagent available / Related diagnostic test / Produced/
stored / Amount supplied nationally
(ml, mg) / Amount supplied internationally
(ml, mg) / Name of recipient OIE Member Countries and of institutions

S.Pullorum antigen

/

Produced

/

500 ml

/

n/a

/

n/a

S. Pullorum control serum /

24mls

/

7.25 ml

/

Commercially sensitive information

Salmonella typing sera /

Produced: 15,102 ml

/

20,295 ml

/

~600 ml

/

Commercially sensitive information

4.Did your laboratory produce diagnostic reagents other than the OIE-approved standard reference reagents?

Yes No

5.Did your laboratory produce vaccines?

Yes No

6.Did your laboratory supply vaccines to OIE Member Countries?

Yes No

ToR:To develop, standardise and validate, according to OIE Standards, new procedures for diagnosis and control of the designated pathogens or diseases

7.Did your laboratory develop new diagnostic methods validated according to OIE Standards for the designated pathogen or disease?

Yes No

8.Did your laboratory develop new vaccines according to OIE Standards for the designated pathogen or disease?

Yes No

ToR:To provide diagnostic testing facilities, and, where appropriate, scientific and technical advice on disease control measures to OIE Member Countries

9.Did your laboratory carry out diagnostic testing for other OIE Member Countries?

Yes No

Name of OIE Member Country seeking assistance / Date
(dd/mm) / No. samples received for provision of diagnostic support (i.e. from surveillance campaign) / No. samples received for provision of confirmatory diagnoses
Hungary / 7 March, 6 July, 31 July, 20 August, 20 September, 12 October / 7
India / 19 April / 1
Saudi Arabia / 14 November / 1

10.Did your laboratory provide expert advice in technical consultancies on the request of an OIE Member Country?

Yes No

Name of the OIE Member Country receiving a technical consultancy / Purpose / How the advice was provided
Argentina / Salmonella control, molecular serotyping / Remote assistance
Belgium / Salmonella control, molecular serotyping, PhD examination / In loco and remote assistance
Cyprus / Salmonella testing and control / Remote assistance
Czech Republic / Salmonella control in hatcheries / Remote assistance
Denmark / Salmonella in turkey flocks / Remote assistance
Ethiopia / Typing of Salmonella isolates / Remote assistance
France / Salmonella control in hatcheries molecular typing / Remote assistance
Germany / Salmonella Gallinarum testing / Remote assistance
Grenada / Salmonella typing / Remote assistance
Hungary / Salmonella control in broilers / Remote assistance
Ireland / Salmonella control in pigs and poultry / Remote assistance
Italy / Salmonella regulation interpretation, testing / Remote assistance
Japan / Identification of equine Salmonella / Remote assistance
Netherlands / Salmonella modelling andcontrol, Salmonella identification / Remote assistance and in loco
New Zealand / Salmonella identification / Remote assistance
Northern Ireland / Salmonella Gallinarumcontrol, general queries, Salmonella control in pigs / Remote assistance and in loco
Poland / Proficiency testing / Remote assistance
Portugal / Salmonella testing andcontrol / Remote assistance and in loco
Russia / Salmonella testing andcontrol / In loco
Saudi Arabia / Identification of Salmonella / Remote assistance
South Africa / Salmonella testing / Remote assistance
Sudan / General advice / Remote assistance
Sweden / Study design, Maldi-Tof / Remote assistance

ToR:To carry out and/or coordinate scientific and technical studies in collaboration with other laboratories, centres or organisations

11.Did your laboratory participate in international scientific studies in collaboration with OIE Member Countries other than the own?

Yes No

Title of the study / Duration / Purpose of the study / Partners (Institutions) / OIE Member Countries involved other than your country
DFID Zoonoses Report / 1 year / Database formulation / various / USA, Italy, France, Spain
Molecular serotyping study / 1 year / Evaluation of molecular methods for serotyping Salmonella / various / Netherlands, Canada
Monophasic ST PCR / 1 year / Validation of mST PCR / Health Protection Agency, IZSVe / Italy
Land crab study / 1 year / Assessment of prevalence of Salmonella in land crabs / St. George’s University, Grenada / Grenada
Salmonella vaccine trial / 2 years / Field study, Salmonella in pigs / IDT Biologica, BPEX / Germany
DISCONTROLS / 2 years / Collection and ranking of disease control and infection / various / France, Germany, Belgium
S. Java study / 1 year / Molecular analysis and comparison of S. Java strains / Veterinary and Agrochemical Research Center / Belgium, Netherlands

ToR:To collect, process, analyse, publish and disseminate epizootiological data relevant to the designated pathogens or diseases

12.Did your Laboratory collect epizootiological data relevant to international disease control?

Yes No

13.Did your laboratory disseminate epizootiological data that had been processed and analysed?

Yes No

14.What method of dissemination of information is most often used by your laboratory?

(Indicate in the appropriate box the number by category)

a)Articles published in peer-reviewed journals:...... 4

b)International conferences:...... 6

c)National conferences:...... 11

d)Other:...... 4

ToR: To provide scientific and technical training for personnel from OIE Member Countries

To recommend the prescribed and alternative tests or vaccines as OIE Standards

15.Did your laboratory provide scientific and technical training to laboratory personnel from other OIE Member Countries?

Yes No

a)Technical visits:...... 6

b)Seminars:...... 20

c)Hands-on training courses:......

d)Internships (>1 month):......

Type of technical training provided (a, b,c or d) / Country of origin of the expert(s) provided with training / No. participants from the corresponding country
a / Russia / 6
b / Belgium / 20
b / Ireland / 7

ToR:To maintain a system of quality assurance, biosafety and biosecurity relevant for the pathogen and the disease concerned

16.Does your laboratory have a Quality Management System certified according to an International Standard?

Yes No

Quality management system adopted
ISO 17025, GLP, ISO 2001

17.Is your laboratory accredited by an international accreditation body?

Yes No

Test for which your laboratory is accredited / Accreditation body
Various Salmonella surveillance and diagnostic tests / UKAS
Various serological and AMR tests / UKAS
Various research methodologies / Lloyds Register

18.Does your laboratory maintain a “biorisk management system” for the pathogen and the disease concerned? (See Manual of Diagnostic Tests and Vaccines for Terrestrial Animals 2012, Chapter 1.1.3 orManual of Diagnostic Tests for Aquatic Animals 2012, Chapter 1.1.1)

Yes No

ToR:To organise and participate in scientific meetings on behalf of the OIE

19.Did your laboratory organise scientific meetings on behalf of the OIE?

Yes No

20.Did your laboratory participate in scientific meetings on behalf of the OIE?

Yes No

ToR:To establish and maintain a network with other OIE Reference Laboratories designated for the same pathogen or disease and organise regular inter-laboratory proficiency testing to ensure comparability of results

21.Did your laboratory exchange information with other OIE Reference Laboratories designated for the same pathogen or disease?

Yes No

22.Was your laboratory involved in maintaining a network with OIE Reference Laboratories designated for the same pathogen or disease by organising or participating in proficiency tests?

Yes No

Purpose of the proficiency tests: (validation of a diagnostic protocol: specify the test; quality control of vaccines: specify the vaccine type, etc.) / Role of your Reference Laboratory (organiser/ participant) / No. participants / Participating OIE Ref. Labs/ organising OIE Ref. Lab.
Assess Laboratory capability to conduct Isolation and identification of Salmonella species and Quality system accreditation
(AHVLA Vetqas PT scheme PT0087 Salmonella in Animal Feed) / Participant / 16 / 1
AHVLA Weybridge UK (participant)
Assess Laboratory capability to conduct Isolation and identification of Salmonella species and Quality system accreditation
(AHVLA Vetqas PT scheme PT0088 Salmonella in Poultry) / Participant / 101 / 2
AHVLA Weybridge UK
Canada
(participants)
Assess Laboratory capability to conduct Isolation and identification of Salmonella species and Quality system accreditation
(AHVLA Vetqas PT scheme PT0090 Control of Salmonella in Poultry Order – run for Defra for UK labs only) / Participant / 28 / 1
AHVLA Weybridge UK
(participant)

23.Did your laboratory collaborate with other OIE Reference Laboratories for the same disease on scientific research projects for the diagnosis or control of the pathogen of interest?

Yes No

Title of the project or contact / Scope / Name(s) of relevant OIE Reference Laboratories
Validation of monophasic Salmonella Typhimurium
( mST) PCRs / Validation of mST PCRs / Italy

ToR:To organise inter-laboratory proficiency testing with laboratories other than OIE Reference Laboratories for the same pathogens and diseases to ensure equivalence of results.

24.Did your laboratory organise or participate in inter-laboratory proficiency tests with laboratories other than OIE Reference Laboratories for the same disease?

Yes No

Purpose for inter-laboratory test comparisons[1] / No. participating laboratories / Participating OIE Member Countries
Assess Laboratory capability to conduct Isolation and identification of Salmonella species and Quality system accreditation. Reports allow comparisons to be made
(AHVLA Vetqas PT scheme PT0087 Salmonella in Animal Feed) / 16 / UK
Assess Laboratory capability to conduct Isolation and identification of Salmonella species and Quality system accreditation
Reports allow comparisons to be made
(AHVLA Vetqas PT scheme PT0088 Salmonella in Poultry) / 101 / UK and Canada
Assess Laboratory capability to conduct Isolation and identification of Salmonella species and Quality system accreditation
(AHVLA Vetqas PT scheme PT0090 Control of Salmonella in Poultry Order – run for Defra for UK labs only) / 28 / UK

ToR: To place expert consultants at the disposal of the OIE

25.Did your laboratory place expert consultants at the disposal of the OIE?

Yes No

Kind of consultancy / Location / Subject (facultative )
Amendment of OIE technical manual chapter / UK / Fowl Typhoid and Pullorum Disease
Commissioned literature review / UK/Italy / Control of Salmonella in food producing animals other than poultry

PUBLICATION LIST 2012 – AHVLA Weybridge

OIE Salmonella Reference Laboratory

Publications in peer-reviewed journals:

  • Salmonellaserovars isolated from table eggs: An overview.Martelli, F. and Davies, R.H. (2012) Food Research International, 45 745–754.
  • Comparison of the environmental survival characteristics of Salmonella Dublin and SalmonellaTyphimurium. Kirchner, M.J. ; Liebana, E.; McLaren, I.; Clifton-Hadley, F.A.; Wales, A.D. ; Davies, R.H. Veterinary MicrobiologyVolume: 159 Issue: 3-4 Pages: 509-514 DOI: 10.1016/j.vetmic.2012.04.009 Published: OCT 12 2012
  • A comparison between longitudinal shedding patterns of SalmonellaTyphimurium and Salmonella Dublin on dairy farms. Kirchner, M.; McLaren, I.; Clifton-Hadley, F.A.; Liebana, E.; Wales, A.D. ; Davies, R.H. Veterinary RecordVolume: 171 Issue: 8 Pages: 194-+ DOI: 10.1136/vr.100865 Published: AUG 25 2012
  • Investigations of the Distribution and Persistence of Salmonella and Ciprofloxacin-Resistant Escherichia coli in Turkey Hatcheries in the UK. Mueller-Doblies D., Clouting C., Davies R.H. Zoonoses Public Health. 2012 Aug 1. doi: 10.1111/j.1863-2378.2012.01524.x. [Epub ahead of print]

PresentationsatInternational Conferences:

  • Characterisation of the phylogenetic relationship of monophasic Salmonellaenterica subsp. enterica (Subspecies I) Serotype 4,[5],12:i:- DT193 and other phage types strains by a complete genome sequencing.Petrovska, L., Connor, T., Harries, S., Davies, R.H., Underwood, A., Wain, J., Woodward, M., Dougan, G. and Kingsley, R. SGM Dublin, 26-28 March 2012
  • Learning Stakeholder Attitudes through an interactive workshop – a case study on Salmonella control in GB pigs. Cook, A., Marier, E., Clifton-Hadley, F.A., Sheehan, R., Hill, A., Davies, R., Armstrong, D., Turvey, K., Rushton, J. & Brunton, L. (2012). 13th conference of the International Society for Veterinary Epidemiology and Economics (ISVEE XIII) 2012, Maastricht, the Netherlands, 20-24 August 2012
  • Using an interactive workshop to learn stakeholder attitudes on Salmonella control in GB pigs. Brunton, L.A., Marier, E., Clifton-Hadley, F.A., Sheehan, R., Hill, A., Davies, R., Armstrong, D., Turvey, K., Rushton, J., Crayford, G. and Cook, A. (2012). Society for Veterinary Epidemiology and Preventive Medicine Annual Meeting 2012, Glasgow, Scotland 28-30 March 2012.
  • Results from Salmonella National Control Programme in chicken laying flocks in GB: 2008 – 2010. Lawes, J.R., Cheney, T., Mueller-Doblies, D. & Davies, R.. Society for Veterinary Epidemiology and Preventive Medicine Annual Meeting 2012, Glasgow, Scotland 28-30 March 2012.
  • Salmonella surveillance data from turkeys – example of the situation in the UK. Mueller-Doblies, D.; 6th turkey science and production conference, Macclesfield, UK, 21-23 March 2012
  • Study on the effect of storage conditions on the recovery of Salmonella from boot swabs and hairnets. Rabie, A., McLaren, I., Sayers, A.R., Vaughan, K., Kell, N. and Davies, R.H. IAFP's European Symposium on Food Safety, Warsaw, Poland, 21-23 May, 2012

PresentationsatNational Conferences

  • Presentation to the industry of OZ0332 findings: British Egg Council headquarters. Martelli, F., 1 March 2012
  • Salmonella in eggs – what’s the actual risk for humans? Martelli, F. AHVLA Salmonella training day, 24-25 January 2012
  • Salmonella in Poultry. Davies, R.H. AHVLA Salmonella training day, 24-25 January 2012
  • Disinfectants and Organic Acids. McLaren, I. AHVLA Salmonella Training day, 24-25 January 2012
  • Salmonella culture in the laboratory. McLaren, I. AHVLA Salmonella Training day, 24-25 January 2012
  • Investigation of sampling sensitivity and sampling/testing issues in the context of the NCP. Gosling, R.J. AHVLA Salmonella Training day 2012.
  • National Control Programmes for Salmonella in Chickens. Mueller-Doblies, D. AHVLA Salmonella Training day 2012.
  • Salmonellaserovars and phage types associated with specific animal reservoirs and their epidemiology. Mueller-Doblies, D. and Davies, R.H.; HPA conference, Warwick University, 11-12 Sept 2012
  • Surveillance and field studies of monophasic Salmonella Typhimurium in food animal production. Gosling, R.J. HPA conference, Warwick University, 11-12 Sept 2012
  • Salmonella associated with captive reptiles and amphibians in Great Britain from 2000 to 2011 - Possible implications on human Salmonellosis. Wu, G., Kidd, S., Davies, R. and Lawes, J. HPA conference, Warwick University, 11-12 Sept 2012
  • Monophasic SalmonellaTyphimurium research. R Davies; Epidemiology of Foodborne Infections Group (EFIG) meeting, 20th June 2012

Otherpresentations:

  • Routes of Transmission - Salmonella in poultry. R. Davies, lecture for MSc students in Veterinary microbiology at the University of Surrey, 19 November 2012
  • Poultry Salmonella Update. R. Davies. AHVLA/SAC Avian Expert Group Science Day, 10 December 2012
  • 2 BPEX/Defra pig NCP roadshows. R. Davies

Annual reports of OIE Reference Laboratories, 20121