Supplementary material 1.Sha1 sequence alignment with Lplantarum bacteriophage by dot-plot calculated using BLAST with default discontiguousMegaBLAST parameters(NCBI).
WCFSp1, prophage of L.plantarumWCFS1,WCFSp2: prophage of L.plantarumWCFS2; JDMp1, prophage of L.plantarumJDM1; JDMp2, prophage of L.plantarumJDM1; Φg1e, temperate phage of L.plantarum;ΦJL-1, virulent phage of L.plantarumMU45; LP65, virulent phage of L.plantarum.
Supplementary material 2
Nucleotide matches between the predicted ORFs of Sha1 and those from the database
ORF# / Strand / Left / Right / Length(bp) / Class / Ident % / Posi % / Gaps % / E-value / Links1 / + / 246 / 1031 / 786 / 1 / 98% / 99% / 0% / 1e-150 / DNA replication protein [Lactobacillus plantarumJDM1] ref|YP_003062563.1|
2 / + / 1167 / 1475 / 309 / 2 / 94% / 96% / 0% / 6e-50 / hypotheticalproteinJDM1_0980 [Lactobacillus plantarumJDM1] ref|YP_003062564.1|
3 / + / 1468 / 1572 / 105 / 2 / 100% / 100% / 0% / 7e-06 / hypothetical protein HMPREF 0531_1090 [Lactobacillus plantarumATCC14917] ref|ZP_04013582.1|
4 / + / 1575 / 2180 / 606 / 1 / 32% / 54% / 7% / 6e-15 / hypothetical protein Lreu23DRAFT_3093 [Lactobacillus reuteri 100-23] ref|ZP_03073817.1|
5 / + / 2245 / 2361 / 117 / 2 / Unknown
6 / + / 2371 / 2565 / 195 / 2 / 38% / 54% / 0% / 8e-06 / prophage Lp4 protein 3, phage-like repressor [Lactobacillus plantarumWCFS1]NP_786616.1
7 / + / 2568 / 2744 / 177 / 2 / 42% / 64% / 2% / 0.59 / hypothetical protein HMPREF 0497_2348 [Lactobacillus buchneriATCC11577] ref|ZP_03943275.1|
8 / + / 2728 / 3150 / 423 / 1 / 49% / 66% / 7% / 1e-25 / hypothetical protein LP65_gp151 [Lactobacillus phage LP65] ref|YP_164786.1|
9 / + / 3147 / 3317 / 171 / 1 / 85% / 91% / 0% / 2e-14 / prophage Lp1 protein 32 [Lactobacillus plantarumWCFS1ref|NP_784420.1|
10 / + / 3298 / 3729 / 432 / 1 / 93% / 96% / 0% / 7e-72 / Phage transcriptional activator RinA [Lactobacillus plantarumJDM1] ref|YP_003062565.1|
11 / + / 4252 / 4422 / 171 / 2 / 85% / 89% / 0% / 6e-17 / putative HNH nuclease [Lactobacillus plantarumJDM1] ref|YP_003062567.1|
12 / + / 4433 / 4903 / 471 / 1 / 92% / 97% / 0% / 8e-84 / restriction endonuclease [Lactobacillus plantarum subsp. PlantarumATCC14917] ref|ZP_04014724.1|
13 / + / 5121 / 5579 / 459 / 1 / 97% / 99% / 0% / 3e-82 / P27 family phage terminase, small subunit [Pediococcusacidilactici7_4] ref|ZP_06197596.1|
14 / + / 5582 / 7480 / 1899 / 1 / 95% / 97% / 0% / 0.0 / Phage terminase-like protein ,large subunit [PediococcuspentosaceusATCC25745] ref|YP_804503.1|
15 / + / 7470 / 7664 / 195 / 1 / 38% / 58% / 0% / 1e-04 / Phage head-tail joining protein [Lactobacillus phageSal2] ref|YP_535179.1|
16 / + / 7667 / 8860 / 1194 / 1 / 95% / 96% / 2% / 0 / possible bacteriophage portal protein [Lactobacillus plantarum subsp. PlantarumATCC14917] ref|ZP_04014729.1|
17 / + / 8838 / 9575 / 738 / 1 / 95% / 96% / 2% / 3e-130 / protease subunit of ATP-dependent Clp protease [PediococcuspentosaceusATCC25745] ref|YP_804500.1|
18 / + / 9575 / 10801 / 1227 / 1 / 92% / 96% / 0% / 0 / HK97 family phage major capsid protein [Pediococcusacidilact7_4] ref|ZP_06197592.1|
19 / + / 10880 / 11218 / 339 / 1 / 87% / 91% / 0% / 4e-46 / phage protein DNA packaging protein [Lactobacillus plantarum subsp. PlantarumATCC14917] ref|ZP_04014732.1|
20 / + / 11202 / 11564 / 363 / 1 / 46% / 66% / 0% / 6e-26 / phage head-tail adaptor [Lactobacillus reuteri 100-23] ref|ZP_03073886.1|
21 / + / 11554 / 11994 / 441 / 1 / 20% / 40% / 16% / 2e-21 / Phage head-tail joining protein [Lactobacillus phage LV-1] ref|YP_002455795.1|
22 / + / 11991 / 12374 / 384 / 2 / 48% / 64% / 0% / 4e-29 / hypothetical protein Lreu23DRAFT_3164 [Lactobacillus reuteri 100-23] ref|ZP_03073888.1|
23 / + / 12375 / 13013 / 639 / 1 / 61% / 77% / 0% / 2e-70 / major tail protein [Lactobacillus reuteri 100-23] ref|ZP_03073889.1|
24 / + / 13215 / 13598 / 384 / 1 / 27% / 53% / 11% / 3e-04 / hypothetical protein Lreu23DRAFT_3166 [Lactobacillus reuteri 100-23] ref|ZP_03073890.1|
25 / + / 13595 / 13786 / 192 / 1 / 39% / 62% / 3% / 0.002 / hypothetical protein JDM1_0995 [Lactobacillus plantarumJDM1] ref|YP_003062579.1|
26 / + / 13799 / 18967 / 5169 / 1 / 41% / 58% / 3% / 6e-111 / putative tape measure protein [Lactobacillus phage Lc-Nu] ref|YP_358771.1|
27 / + / 19039 / 20811 / 1773 / 1 / 34% / 49% / 10% / 1e-26 / minor structural protein gp75 [Leuconostoc citreumKM20] ref|YP_001728316.1|
28 / + / 20881 / 23250 / 2370 / 1 / 37% / 56% / 4% / 9e-141 / minor structural protein gp89 [Lactobacillus phage LL-H] ref|YP_001285899.1|
29 / + / 23267 / 26056 / 2790 / 1 / 69% / 79% / 1% / 0 / tail fiber [Lactobacillus plantarumJDM1] ref|YP_003062583.1|
30 / + / 26049 / 26303 / 255 / 1 / 90% / 91% / 5% / 1e-27 / hypothetical protein HMPREF 0531_2251 [Lactobacillus plantarumATCC14917] ref|ZP_04014743.1|
31 / + / 26307 / 26468 / 162 / 1 / 94% / 100% / 0% / 2e-19 / hypothetical protein HMPREF 0531_2252 [Lactobacillus plantarumATCC14917] ref|ZP_04014744.1|
32 / + / 26452 / 27336 / 885 / 1 / 99% / 92% / 1% / 1e-71 / prophage Lp2protein53 [Lactobacillus plantarum subsp. PlantarumATCC14917] ref|ZP_04014745.1|
33 / + / 27352 / 28524 / 1173 / 1 / 85% / 90% / 0% / 2e-155 / endolysin[Lactobacillus plantarum subsp. plantarumATCC14917] ref|ZP_04014747.1|
34 / + / 28524 / 28787 / 264 / 1 / 36% / 56% / 8% / 0.002 / phage-related holin [Lactobacillus rhamnosusGG] ref|YP_003171267.1|
35 / + / 28800 / 29330 / 531 / 1 / Unknown
36 / - / 30182 / 31318 / 1137 / 2 / 50% / 68% / 1% / 1e-99 / phage integrase [Lactobacillus hilgardiiATCC8290] ref|ZP_03952344.1|
ORF# / Strand / Left / Right / Length(bp) / Class / Ident % / Posi % / Gaps % / E-value / Links
37 / - / 31432 / 31794 / 363 / 2 / 27% / 51% / 6% / 6e-17 / hypothetical protein LVIS_0718 [Lactobacillus brevis ATCC 367] ref|YP_794892.1|
38 / - / 31962 / 32276 / 315 / 2 / 37% / 61% / 10% / 4e-04 / hypothetical protein LCABL_12890 [Lactobacillus casei BL23] ref|YP_001987231.1|
39 / - / 32269 / 32682 / 414 / 2 / Unknown
40 / - / 32835 / 33023 / 189 / 2 / 98% / 100% / 0% / 1e-25 / hypothetical protein HMPREF 0531_2199 [Lactobacillus plantarumATCC14917] ref|ZP_04014691.1|
41 / - / 33339 / 33539 / 201 / 2 / 100% / 100% / 0% / 1e-26 / hypothetical protein HMPREF 0531_2200 [Lactobacillus plantarumATCC14917] ref|ZP_04014692.1|
42 / - / 33593 / 34585 / 993 / 2 / 26% / 47% / 6% / 7e-04 / prophage Lp1protein5 [Lactobacillus plantarumWCFS1] ref|NP_784394.1|
43 / - / 34710 / 35414 / 705 / 2 / 44% / 68% / 1% / 7e-37 / bifunctional S24 family peptidase/transcriptional regulator [Lactobacillus buchneriATCC11577] ref|ZP_03942483.1|
44 / + / 35583 / 35816 / 234 / 2 / 66% / 78% / 0% / 2e-20 / Cro family transcriptional regulator [Streptococcus phage 10270.2] ref|YP_598411.1|
45 / + / 35832 / 36542 / 711 / 1 / 42% / 57% / 15% / 2e-42 / phage-related antirepressor [Lactobacillus rhamnosusLc705] ref|YP_003173532.1|
46 / + / 36555 / 36686 / 132 / 1 / 47% / 77% / 2% / 0.47 / hypothetical protein LCABL_10890 [Lactobacillus casei BL23] ref|YP_001987032.1|
47 / - / 36658 / 36984 / 327 / 1 / 31% / 47% / 9% / 1e-22 / conserved hypothetical protein [Lactobacillus johnsoniiATCC
33200] ref|ZP_04007995.1|
48 / + / 37078 / 37407 / 330 / 2 / 50% / 66% / 2% / 1e-24 / protein HMPREF 0519_0013 [Lactobacillus hilgardiiATCC8290] ref|ZP_03952353.1|
49 / + / 37401 / 37484 / 84 / 2 / 65% / 65% / 20% / Unknown
50 / + / 37497 / 37676 / 180 / 2 / 100% / 100% / 0% / 4e-25 / prophage Lp3protein4 [Lactobacillus plantarumJDM1] ref|YP_003062554.1|
51 / + / 37710 / 37976 / 267 / 1 / 77% / 80% / 6% / 4e-30 / hypothetical protein HMPREF 0531_2212 [Lactobacillus plantarumATCC14917] ref|ZP_04014704.1|
52 / + / 37979 / 38179 / 201 / 2 / 92% / 98% / 0% / 1e-27 / hypothetical protein JDM1_0972 [Lactobacillus plantarumJDM1] ref|YP_003062556.1|
53 / + / 38191 / 38415 / 225 / 2 / Unknown
54 / + / 38464 / 38610 / 147 / 1 / Unknown
55 / + / 38610 / 39143 / 534 / 1 / 37% / 57% / 4% / 1-17 / phage protein [Clostridium botulinum E1 str.' BoNT E Belug`] ref|ZP_04822292.1|
56 / + / 39152 / 39814 / 663 / 1 / 73% / 85% / 0% / 2e-91 / phage nucleotide-binding protein [Lactobacillus buchneriATCC11577] ref|ZP_03942528.1|
57 / + / 39816 / 40475 / 660 / 1 / 88% / 91% / 2% / 7e-109 / hypothetical protein phig1ep56 [Lactobacillus phage phig1e] ref|NP_695185.1|
58 / + / 40522 / 41214 / 693 / 2 / 91% / 95% / 0% / 4e-123 / hypothetical protein JDM1_0977 [Lactobacillus plantarumJDM1] ref|YP_003062561.1|