NSCI 6150- Methods in Neuroscience

Fall 2016 WF 2:00pm-3:50pm

Instructor: Dr. Sara M. Clark

Email:

Office: 1008D Stern

Office Hours: By Appointment

Required Text: None. Handouts, book chapters, and journal articles may be assigned via Blackboard as necessary.

Course Overview and Goals

This course exposes students to contemporary theories and techniques used by Tulane neuroscientists in their own research programs. Students will gain knowledge of techniques used to study the nervous system from all levels of inquiry ranging from the study of molecules and cells to animal behavior and recording of human brain activity. Students will demonstrate their proficiency in the subject by completing four written exams as well as a series of assignments.

Make-up Exams

Failure to take an exam at the scheduled day and time will result in a score of 0 (zero) on that exam. If a student misses a test for any valid reason, documentation must be provided (either written or via email) prior to the test. Makeups are not guaranteed to be similar in format to the test administered on the scheduled day. If a student misses more than one test, both with valid reasons, a cumulative makeup test at the end of the semester will be awarded, on a case-by-case basis. Again, this test may not be similar to the scheduled test and may follow any format, including non-written formats.

Honor Code:

All students are expected to be familiar with and are required to adhere to all tenets of the Honor Code of Tulane University.

Syllabus: (note: subject to change as required)

Date Title Note

8/31 (W) Introduction and OverviewAssignment #1

9/2 (F) Ethics Guest Lecturer- Dr. Wee

9/7 (W) Choosing an Animal ModelAssignment #1 Due

9/9 (F) Behavior

9/14 (W)Behavior

9/16 (F) Behavior Assignment #2

9/21 (W)Antibodies and Tissue PrepAssignment #2 Due

9/23 (F) Exam #1- Ethics through Behavior

9/28 (W) Immuno. and Microscopy

9/30 (F) Proteins

10/5 (W)ProteinsAssignment #3

10/7 (F) NO CLASS- BRAIN INSTITUTE- Go to Dr. Gage Lecture

10/12 (W)NO CLASS- YOM KIPPUR

10/14 (F) NO CLASS- FALL BREAK

10/19 (W) ElectrophysiologyGuest Lecturer- Grant Weiss

10/21 (F) Exam #2- Antibodies through ProteinsAssignment #3 Due

Date Title Note

10/26 (W) TBA

10/28 (F)Cell CultureGuest Lecturer- Dr. Rainville

11/2 (W) Viruses and Assorted Vectors

11/4 (F) Pharmacology, DREADS, RASSLsAssignment #4

11/9 (W)Hormone AssaysAssignment #4 Due

11/11 (F) Exam #3- TBA through Pharmacology

11/16 (W)Miscellaneous Assays

11/18 (F)Human Imaging

11/23 (W)NO CLASS- THANKSGIVING BREAK

11/25 (F) NO CLASS- THANKSGIVING BREAK

11/30 (W) Genome Methods/CRISPRGuest Lecturer- Dr. Westmoreland

12/2 (F) PCRs and RelatedGuest Lecturer- Dr. Earls

12/7 (W)EpigeneticsGuest Lecturer- Dr. Schrader

12/9 (F) TBAAssignment #5?

Exam IV (Assays through TBA), Monday, December 12 9am-noon

Course Requirements:

Attendance: Attendance at each class meeting is required. If you must miss class, please email Sara to let her know. Excessive unexcused absence (defined as missing three or more classes) may result in lowering of the grade by one letter.

Exams: There will be four exams. Each exam will account for 20% of the final grade. Exams are non-cumulative. The exam format is multiple choice, fill in the blank, and short answer questions.

Assignments: There will be 4-5 assignments given during the semester. Some assignments may be completed in class, but some may not. The assignments are all equally weighted and will together make up 15% of the final grade.

Ethics: Completion of an online ethics course and associated quiz(zes) is required for this course. This will also account for 5% of your final grade. More details will be provided in class.

Questions/Concerns:

Please make an appointment to see me. I will be happy to answer questions or talk about any concerns you might have. You can best reach me by email at: . My office is located in 1008D Stern, which is located in the basement of Stern off of the kitchen area.