Yun-Zheng Le, Ph.D.

Department of Medicine Endocrinology & Harold Hamm Diabetes Center

University ofOklahomaHealthSciencesCenter

941, Stanton L. Young Blvd., BSEB 302G

Oklahoma City, OK73104, USA

Tel: (405) 271-1087; E-mail: ;

EDUCATION

Sept 2004-June 2005:Faculty Leadership Program, the University of Oklahoma Health Sciences Center, USA.

Oct. 1988-May 1994: Ph.D., Biology/Microbiology (Advisor: Dr. Leo C. Vining, FRSC), Dalhousie University, Canada.

Sept. 1978-July, 1982: B. Sc. Biochemistry, Fudan University, China.

PROFESSIONAL EXPERIENCE

June 2017-present:Harold Hamm Chair in Diabetes Research, Harold Hamm Diabetes Center, the University of Oklahoma Health Sciences Center, USA.

July 2012-June 2017:Inaugural Choctaw Nation Chair in Diabetes Research, Harold Hamm Diabetes Center, the University of Oklahoma Health Sciences Center, USA.

July 2011-present:Associate Professor of Medicine (tenured in 2013) and Adjunct Associate Professor of Cell Biology and Ophthalmology (2014), College of Medicine and Graduate, the University of Oklahoma Health Sciences Center, USA.

Jan. 2007-June 2011:Assistant Professor of Medicine (Tenure-track),College of Medicine, the University of Oklahoma Health Sciences Center, USA.

Jan. 2007-June 2011:Adjunct Assistant Professor of Cell Biology, Colleges of Medicine and Graduate, the University of Oklahoma Health Sciences Center, USA.

Sept. 2002- Dec. 2006:Principal Investigator, Dean A. McGee Eye Institute, USA.

Sept. 2000- Dec. 2006:Assistant Professor of Research, Department of Cell Biology, the University of Oklahoma Health Sciences Center, USA.

Sept. 1998-Aug. 2000:Senior Research Scientist with Dr. Brian Sauer, Developmental Biology Program, Oklahoma Medical Research Foundation, USA.

Sept. 1996-Aug. 1998:Visiting Fellow (Advisor: Dr. Brian Sauer), Laboratory of Biochemistry and Metabolisms, National Institute of Diabetes, Digestive and Kidney diseases//NIH, USA.

June 1994-Aug. 1996: Postdoctoral Fellow (Advisor: Dr. Melanie J. Dobson), Biochemistry Department, Dalhousie University, Canada.

Sept 1988-May 1994:Graduate Research Assistant (Advisor: Dr. Leo C. Vining, Royal Society Fellow of Canada) and Teaching Assistant, Biology Department, Dalhousie University, Canada.

Aug. 1982-Sept. 1988: Engineer and Group Leader of Biochemical Reagent, Dong Feng Biochemical Reagent Co., Shanghai Institute of Biochemistry, Chinese Academy of Sciences, Shanghai, China.

Awards, Honors, Special Recognition

2017-present: Harold Hamm Endowed Chair in Diabetes Research, Harold Hamm Diabetes Center, the University of Oklahoma Health Sciences Center, USA.

2014-2017:Visiting Professorship, Shanghai Jiaotong University School of Medicine, Shanghai, China.

2012-2017Inaugural Choctaw Nation Endowed Chair in Diabetes Research, University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma, USA.

2012: Loris and David Rich Endowed Lectureship in Visual Sciences, Department of Ophthalmology, University of Alabama at Birmingham, Birmingham, Alabama. USA.

2011:American Diabetes Association Magazine, Forefront, featured Investigator.

2010-2012: Visiting Professorship, School of Ophthalmology and Optometry, Wenzhou Medical College, China.

2009-2012:Task Group Member, Arnold and Mabel Beckman Initiative for Macular Research.

2008-2012:Visiting Professorship, Department of Ophthalmology, Xiangya Hospital of Central South University, China.

2007: Award for Outstanding Contribution to Vision Research (unsolicited), Hope for Vision of Washington D.C.

2006: Young Investigator Travel Award, the XII International Symposium on Retinal Degeneration, 2006.

1998: NIH Fellows Award for Research Excellence(of 120 in ~3,000 NIH fellows), National Institutes of Health.

1996-1998: Visiting Fellowship, National Institutes of Health, National Institute ofDiabetes, Digestive and Kidney Diseases.

PROFESSIONAL ACTIVITY

Professional Memberships

2006-Present: American Diabetes Association.

2001–Present: Association for Research in Vision and Ophthalmology.

2001-2015:American Association for the Advancement of Science.

2010–2012: International Society for Eye Research.

2004-2006: American Society for Biochemistry and Molecular Biology.

Service to Scientific Community

2016: Grant Reviewer Advisor, Kentucky Science and Engineering Foundation

2009-2011: Member, Imaging, Stem Cell, and Cell Biology Task Groups, Arnold and Mabel Beckman Initiative for Macular Research (on atrophic age-related macular degeneration).

2007-present: Moderatorof Regular Sessions, and Organizer and Co-Organizer and Moderator of Special Interest Groups: Annual Meeting of the Association for Research in Vision and Ophthalmology.

2004-present: Cre-drive mouse line distributor to vision research community.

2004-2007: Cre/lox plasmids distributor for NIH/NIDDK.

Grant Review

2017: Ad Hoc Grant Reviewer, NIH Disease and Pathology of Visual System Study Section

2017: Ad Hoc Reviewer for Project Grants, Fight for Sight, United Kingdom.

2015-2017: Ad Hoc Grant Reviewer, British MRC Neurosciences Research and Fellowship Grant Panel

2015-2016: Member, Harold Hamm Diabetes Center grant review committee

2014: Ad Hoc Grant Reviewer, NIH/NEI Special Emphasis Panel on Retinopathy

2013: Ad Hoc Grant Reviewer, NIH Biology of Visual System Study Section

2013-14: Ad Hoc Grant Reviewer: Oak Ridge Associated Universities

2012-2013: Ad Hoc Grant Reviewer, Diabetes United Kingdom

2012: Ad Hoc Grant Reviewer, Foundation Fighting Blindness of Canada.

2009: Ad Hoc Grant Reviewer, NIH Cell Biology Integrated Review Group.

2006-present: Ad Hoc Grant Reviewer, Presbyterian Health Foundation.

2006-present: Ad Hoc Grant Reviewer, University of Oklahoma College of Medicine alumni association.

Editorial Service

2016-present:Editor, Special Issue on Diabetic Retinopathy, Vision Research

2011-present: Editorial board member, World Journal of Ophthalmology

2012-present:Editorial board member, Data Sets in Medicine (Ophthalmology)

Manuscript Review

2017-present: Federation of American Societies for Experimental BiologyJournal

2017-present: Proceedings of the National Academy of Sciences (USA)

2017-present: Visual Neuroscience

2015-present: Scientific Report (Nature Group)

2015-present:Journal of Chemical Neuroanatomy

2015-present:Molecular and Cellular Endocrinology

2015-present:International J Ophthalmology and Eye Science

2015-present:International J diabetes

2014-present:Endocrine

2014-present:European J Pharmacology

2013-present:American Journal of Physiology-Heart and Circulatory Physiology

2013-present:Frontier in Physiology

2013-present:World Journal of Ophthalmology

2011-present: Journal of Inflammation Research

2011-present:Journal Neuroinflammation

2010-present: Experimental Eye Research

2011-present:Experimental Diabetes Research

2010-present: Investigative Ophthalmology & Visual Science

2010-present: Journal of Ophthalmology

2010-present:Journal of Visualized Experiments

2010-present: Laboratory Investigation (Nature Group)

2010-present: Letters in Drug Design & Discovery

2010-present: Molecular and Cellular Biochemistry

2010-present:Molecular Vision

2010-present: Ophthalmic Research

2010-present:PLoS One

2009-present: Journal of Cellular and Molecular Medicine

2008-present: Diabetes

2008-present: Vision Research

Consultantships

2007-2012:Allergan Inc. Irvine, California. USA.

PUBLICATIONS

Journals

  1. Hartnett, M.E. Baehr W., Le, Y. (2017) Diabetic Retinopathy, an Introduction, Vision Res (in press).
  2. Hartnett, M.E. Le, Y., Baehr W. (2017) Editorial to “Diabetic Retinopathy”, a Special Issue of Vision Research., Vision Res (in press)
  3. Le, Y.* (2017) VEGF production and signaling in Müller glia are critical to modulating vascular function and neuronal integrity in diabetic retinopathy and hypoxic retinal vascular diseases. Vision Res (in press).
  4. Le, Y.* Fu, S.*, Dong, S., Zhu, M. (2017) VEGF as a trophic factor for Müller glia in hypoxic retinal diseases. Adv. Exp Med Biol. (in press).
  5. Butler, M.R., Ma, H., Yang, F., Belcher, J., Le, Y., Biel, M., Michalakis, S., Iuso, A., Krizaj, D., Ding, X.Q. (2017). Endoplasmic reticulum (ER) Ca2+-channel activity contributes to ER stress and cone death in cyclic nucleotide-gated channel deficiency. J. Biol. Chem. (in press).
  6. Hu, J.*, Li, T., Du, X., Wu, Q., Le, Y.*(2017). G protein-coupled receptor 91 signaling in diabetic retinopathy and hypoxic retinal diseases. Vision Res.(in press).
  7. Zhang, Y., Cross, S. D., Lee, Y., Stanton, J. B., Marmorstein, A. D., Le, Y. Marmorstein, L. Y. (2017). RPE-specific deletion of murine Atg5 or Atg7 causes AMD-like features and retinal degeneration. Mol.Vision, 23, 228-241. PMID: 28465655.
  8. *Le Y, Zhu M, Anderson, RE (2016) Cre recombinase: you can’t live with it, and you can’t live without it. Adv Exp Med Biol.854:725-30.
  9. Biswal M.R., Idelfonso C., Mao H., Seo S.J., Wang Z., Li H., Le Y, Lewin AS. (2016) Conditional Induction of Oxidative Stress in RPE: a Mouse Model of Progressive Retinal Degeneration. Adv Exp Med Biol.854:31-7.
  10. Grabner C., Gandini M., Rehak R., Le Y., Zamponi GW., Schmitz, F. (2015) RIM1/2 Mediated Facilitation of Cav1.4 Channel Opening is Required for Ca2+-Stimulated Release in Mouse Rod Photoreceptors. J. Neurosci. 23;35(38):13133-47. doi: 10.1523/JNEUROSCI.0658-15.2015.
  11. Li L, Rao KN, Le Y, Hurd TW, Lillo C, Khanna H (2015) Loss of Retinitis Pigmentosa 2 (RP2) protein affects cone photoreceptor sensory cilium elongation in mice. Cytoskeleton72(9):447-54 PMC4715527
  12. Fu S, Dong, S, Zhu M, Sherry DM, Wang Y, You Z Haigh JJ, *Le Y (2015) Müller glia are a major cellular source of survival signals for retinal neurons in diabetes. Diabetes64(10):3554-63.PMC4587642.
  13. Sahu B, Sun W, Perusek L, Parmar V, Le Y, Griswold MD, Palczewski K, Maeda A (2015) Conditional Ablation of Retinol Dehydrogenase 10 in the Retinal Pigmented Epithelium Causes Delayed Dark Adaption in Mice. J. Biol. Chem. 290(45):27239-47. doi: 10.1074/jbc.M115.682096.
  14. Perusek L, Sahu B, Parmar T, Maeno H, Aria E, Le Y, Subauste C, Chen Y, Palczewski K, Maeda A (2015) A2E accumulation and visual function are independent of Atg7-mediated autophagy in the retinal pigmented epithelium. J. Biol. Chem. (290(48):29035-44. doi: 10.1074/jbc.M115.682310.
  15. Wang J., Zhu M., *Le Y. (2015) Müller cell-derived VEGF in diabetic retinopathy. World J. Diabetes.6(5):726-33. doi: 10.4239/wjd.v6.i5.726.
  16. Venkatesh A, Ma S, Le Y, Hall MN, Rüegg MA, Punzo C. (2015) Activated mTORC1 promotes long-term cone survival in retinitis pigmentosa mice., J Clin Invest. 125 (4):1446-58.PMC4396488
  17. Ma S, Venkatesh A, Langellotto F, Le Y, Hall MN, Rüegg MA, Punzo C. (2015) Loss of mTOR signaling affects cone function, cone structure and expression of cone specific proteins without affecting cone survival. Exp Eye Res. 135:1-13. PMC4446177
  18. Fu S., Zhu M., Wang Y. *Le Y. (2014) Efficient induction of productive Cre-mediated recombination in RPE. (Invited Research Article) Mol Vis 20:480-487. PMC3984040.
  19. Dong S., Liu. Y.; Zhu, M., Xu, X., *Le, Y. (2014) Simplified system to investigate alteration of retinal neurons in diabetes. Adv Exp Med Biol. 801:139-43. (doi: 10.1007/978-1-4614-3209-8_18). PMID: 24664691.
  20. Zhou K.K., Benyajati S., Le Y., Cheng R., Zhang W., Ma J.X. (2014). Interruption of Wnt signaling in Müller cells ameliorates ischemia-induced retinal neovascularization. 9(10):e108454. (doi: 10.1371/journal.pone.0108454), PMC4182699.
  21. Ramamurthy V., Jolicoeur C., Koutroumbas D., Mühlhans J., Le Y., Hauswirth, W.W., Gießl A., Cayouette M. (2014) Numb regulates the polarized delivery of cyclic nucleotide-gated ion channels in rod photoreceptor cilia, J. Neurosci. 34:13976 –13987.
  22. Mao H., Seo S.J., Biswal M.R., Li H., Conners M., Nandyala A., Jones K., charffetter-Kochanek K., Le Y., Lewin A.S. (2014) Mitochondrial oxidative stress in the retinal pigment epithelium leads to localized retinal degeneration. Invest. Ophthalmol. Vis. Sci. 55:4613-27. (doi: 10.1167/iovs.14-14633).PMC4112607
  23. Fu S., Zhu M., Ash J.D., Wang Y. *Le Y. (2014) Investigating the role of retinal Müller cells with approaches in genetics and cell biology., Adv Exp Med Biol. 801:401-5. (doi: 10.1007/978-1-4614-3209-8_51) PMID: 24664724.
  24. Luo L., Uehara H., Olsen T., Das K.S., Zhang X., Simonis J.M, Jackman K., Archer B., Singh N., Miya T., Huang W., Ahmed F., Liu Y., Ambati J., Mamalis C., Chiodo V.A., Hauswirth W.W., Ferrara N., Baffi J., Gao G., Zygmunt K. Whitaker R., Lacal P.M., Orecchia A., Fu Y., Le Y., Jones A.D., Owen L., Holt D., Albuquerque R., Baehr W., Thomas K., Li D., Chalam K.C., Shibuya. M., Grisanti S., Wilson D.J., Ambati B.K. (2013). Photoreceptor Avascular Privilege is shielded by soluble VEGF receptor-1. eLife 2:e00324 (doi: 10.7554/eLife.00324). PMC3687373.
  25. Nasonkin I.O., Merbs S.L., Lazo K., Oliver V.F., Brooks M., Patel K., Enke R.A., Nellissery J., Jamrich M., Le Y., Bharti K., Fariss R.N., Rachel R.A., Zack D.J., Rodriguez-Boulan E.J., Swaroop R. (2013) Conditional knockdown of DNA methyltransferase-1 (Dnmt1) reveals a key role of retinal pigment epithelium integrity in photoreceptor outer segment morphogenesis, Development140: 1330-41. PMC3585665.
  26. Chen Y., Sawada O., Kohno H., Le Y., Subauste C., Maeda T., Maeda A. (2013) Autophagy protects the retina from light-induced degeneration J. Biol. Chem. 288: 7506-18. PMC3597791
  27. He C., Zhu H., Zhang W., Okon I., Wang Q. Li H., Le Y. , Xie Z. (2013) 7-ketocholesterol Induces Autophagy in Vascular Smooth Muscle Cells through Nox4 and Atg4B. J. Am. Pathol. 183:626-37. PMC3730774.
  28. Siegert S., Cabuy E., Scherf B.G., Kohler H., Panda S., Le Y., Fehling H.J., Gaidatzis D., Stadler M.B., Roska B. (2012) Optional code and disease map for adult retinal cell types. Nat Neurosci. 15:487-95. (doi: 10.1038/nn.3032). PMID:22267162.
  29. Lin M., Hu Y., Chen Y., Zhou K., Jin J., Zhu M., Le Y., Ge J., Ma J.X. (2012) Impacts of Hypoxia-inducible Factor-1 Knockout in the Retinal Pigment Epithelium (RPE) on Choroidal Neovascularization. Invest. Ophthalmol. Vis. Sci. 53:6197–6206. PMC4113187.
  30. Zhu, M., Bai, Y., Zheng, L. *Le, Y. (2012) Presence of RPE-produced VEGF in a timely manner is critical to choroidal vascular development. Adv Exp Med Biol. 723:299-304. PMID:22183346
  31. Zhong Y., Li J. Wang, J.J., Chen C., Tran JT, Saadi A, Yu Q, Le Y., Mandal N.A. Anderson R.E., Zhang S.X. (2012) X-box binding protein 1 is essential for the anti-oxidant defense and cell survival in the retinal pigment epithelium. PLoS One7(6):e38616.PMC3371004.
  32. Xu H., Liu J., Xiong S. Le Y., Xia X. (2012) Suppression of Retinal Neovascularization by Lentivirus-Mediated Netrin-1 Small Hairpin RNA. Ophthalmic Res 47:163-169 (DOI:10.1159/000331428). PMC3251241.
  33. Xu H., Song Z., Fu S., Zhu M., *Le Y. (2012) RPE barrier breakdown in diabetic retinopathy: seeing is believing. J. Ocular Biology, Diseases and Informatics 4:83–92. (DOI 10.1007/s12177-011-9068-4). PMC3342408.
  34. Keady B., Le Y., Pazour G (2011) IFT20 is required for opsin trafficking and photoreceptor outer segment development. Mol. Biol. Cells22: 921-30.PMC3069017.
  35. *LeY. (2011) Conditional gene targeting: dissecting the cellular mechanisms of retinal degenerations. J. Ophthalmol. (doi:10.1155/2011/806783).PMC3021885.
  36. Lin M., Chen Y., Jin J., Hu Y., Zhou K., Zhu M., Le Y., Ge J., Ma J.X. (2011) The role of retinal Müller cell-derived hypoxia-inducible factor-1 in retinal inflammation and neovascularization in diabetic retinopathy. Diabetologia54:1554-66.
  37. *Kusari J. (co-corresponding author), Padillo E.U., Zhou S., Ni M., Lalwani K., Bai Y. Wang J., Song, Z. Zhu M. *Le Y. (co-corresponding author), Gil D.W. (2011) Effect of Brimonidine on Retinal and Choroidal Neovascularization in Animal Models of Age-Related Macular Degeneration and Retinopathy of Prematurity. Invest. Ophthalmol. Vis. Sci. 52:5424-31. PMC3176033
  38. Xu H., *Le Y. Visualizing diabetes- and ischemia-induced breakdown of outer blood-retina barrier (2011) Invest. Ophthalmol. Vis. Sci. 52:2160-4. PMC3080181.
  39. Ivanovic I., Anderson R.E., Le Y., Fliesler S., Sherry D., Rajala R. (2011) Deletion of the p85α Regulatory Subunit of Phosphoinositide 3-Kinase in Cone Photoreceptor Cells Results in Cone Photoreceptor Degeneration. Invest. Ophthalmol. Vis. Sci. 52:3775-83. PMC3109053.
  40. Lange C., Heynen S., Tanimoto, N., Le Y., Meneau I., Seeliger M.W., Samardzija M., Grimm C. (2011) Normoxic activation of hypoxia inducible factors in photoreceptors provides transient protection against light induced retinal degeneration Invest. Ophthalmol. Vis. Sci. 52:5872-80.
  41. Ivanovic I., Rajala A., Gupta V., Dighe R., Le Y., Anderson R.E., Rajala R. (2011) Phosphoinositide 3-Kinase Signaling in Retinal Rod Photoreceptors. Invest. Ophthalmol. Vis. Sci. 52:6355-62. PMC3176013
  42. Liu J., Xia X., Xiong S. Le Y., Xu H. (2011) Intravitreous high expression level of netrin-1 in patients with proliferative diabetic retinopathy. Eye Science (Yan Ke Xue Bao) 26 (2):35-42.PMC3445338.
  43. *Le Y. (2010) Computer-assisted semi-quantitative analysis of choroidal density. Adv Exp Med Biol.664:349-53. PMID: 20238035
  44. Wang J., Xu X., Elliott M.H. *Le Y. (2010) Müller cell-derived VEGF is essential for diabetes-induced retinal inflammation and vascular leakage.Diabetes59: 2297-2305. PMID: 20530741, PMC2927953.
  45. *Le, Y. Bai, Y., Zhu, M., Zheng, L. (2010) Temporal requirement of RPE-derived VEGF in the formation of choroidal vasculature. J. Neurochem.112: 1584–1592. PMID: 20067573; PMC2933813.
  46. Zhu M., Zheng L., Ueki Y., Ash J.D., *Le Y. (2010) Unexpected transcriptional activity of the human VMD2 promoter in retinal development. Adv Exp Med Biol.664: 211-216. PMID: 20238019
  47. Bai Y., Ma J., Guo J., Wang J., Zhu M., Chen Y., *Le Y. (2009) Müller cell-derived VEGF is a significant contributor to retinal neovascularization (cover article). J. Pathol.219:446-454. PMID: 19768732
  48. Ueki Y.,Le Y., Chollangi S., Müller, W., Ash J.D., (2009) Preconditioning-induced protection of photoreceptors requires cell autonomous activation of the signal-transducing receptor gp130. Proc Natl. Acad. Sci. USA106: 21389–21394. PMID: 19948961; PMC2785722.
  49. Avasthi P., Watt C., Williams D., Le Y., Li S., Chen C., Frederick J., Baehr, W. (2009) Heterotrimeric Kinesin-II Mediates Trafficking of Membrane Proteins to Cone, but not Rod Outer Segments. J. Neurosci29:14287–14298. PMID: 19906976; PMC2788486.
  50. Haruta M., Bush R.A., Kjellstrom, S., Le Y., Camasamudram V., Sieving P.A. (2009) Depleting Rac1 component of NADPH oxidase in mouse photoreceptors protects from photo-oxidative stress without affecting rod structure. Proc Natl. Acad. Sci. USA. 106:9397-9402. PMID: 19470639; PMC2685247.
  51. Ueki Y., Ash J.D.,Zhu M., Zheng, L., *Le Y. (2009) Expression of Cre recombinase in the retinal Müller cells (cover article). Vis. Res.49: 615–621 (doi:10.1016/j.visres.2009.01.012). PMID: 19948109; PMC2787478.
  52. Li F., Wicker L.D., Brush R.S., Elliott M.H., Le Y., Henry K.A., Anderson A.G., Zhao C., Sun X., Zhang. K., Anderson R.E. DHA does not protect ELOVL4 transgenic mice from retinal degeneration (2009) Mol. Vis. 15:1185-1193 PMID: 19536303; PMC2697457.
  53. Thiersch M., Lange C., Joly S., Heynen S., Le Y., Samardzija, M. Grimm C. (2009) Retinal neuroprotection by hypoxic preconditioning is independent of HIF-1 expression in photoreceptors. European J. Neurosci 29: 2291–2302. PMID: 19508692
  54. *LeY., Zheng W., Rao P., Zheng L., Anderson R.E., Esumi N., Zack, J.D., Zhu M., (2008) Inducible expression of Cre recombinase in the RPE. Invest Ophthalmol Vis Sci.49: 1248-1253. PMID: 18326755; PMC2711689.
  55. Rajala A., Tanito M, Le Y., Kahn. R. C., Rajala, R.V. (2008) Loss of neuroprotective survival signal in mice lacking insulin receptor gene in rod photoreceptor cells. J. Biol Chem283:19781-92. PMID: 18480052; PMC2443670.
  56. Zheng L., Anderson R.E., Agbaga M., Rucker III E.B., *LeY. (2006) Loss of BCL-XL; increased rod photoreceptor susceptibility to bright light damage. Invest Ophthalmol Vis Sci.47: 5583-5589. PMID: 17122152.
  57. *Le Y., Zheng L., Zheng, W., Ash, J.D., and Anderson, R. E. (2006) Mouse opsin promoter controlled expression of Cre recombinase in transgenic mice, Mol Vis12: 389-398. PMID: 16636658.
  58. *Le Y., Ash, J.D., Al-Ubaidi, M.R., Chen, Y., Ma, J., and Anderson, R. E. (2004) Targeted expression of Cre recombinase to cone photoreceptors in transgenic mice Mol Vis10:1011-1018. PMID: 15635292.
  59. Le Y., Gagneten, S., Larson, T., Santha, E., Dobi, A., Agoston, D. and Sauer B. (2003) Far-upstream elements are dispensable for tissue-specific proenkephalin expression using a Cre-mediated knock-in strategy. J. Neurochem. 84: 689-697. PMID: 12562513
  60. Le Y. and Sauer, B. (2000) Conditional gene knockout using Cre recombinase. In Methods in Molecular Biology: Protocol in Developmental Biology, Tuan R.S. and Lo C.W. eds. Humana Press, Totowa, NJ. 136: II,477-485. PMID: 10840735.
  61. Le, Y., Miller, J.and Sauer, B. (1999). GFPcre Fusion Vectors with Enhanced Expression. Anal. Biochem., 270:334-336. PMID: 10334853.
  62. Le, Y.,Gagneten, S., Tombaccini, D., Betheke, B. and Sauer, B. (1999). Nuclear targeting determinants in the Cre recombinase of phage P1. Nucleic Acids Res., 27:4703-4709. PMID: 10572169.
  63. Chute, I., Le, Y., Ashley, T. and Dobson, M.J.(1997)The telomere-associated DNA from the short arm of human chromosome 20 contains a pseudotelomere structure and shares sequences with the subtelomeric regions of 4q and 18p. Genomics, 46, 51-60. PMID: 9403058
  64. Gagneten, S., Le Y., Miller, J.and Sauer, B. (1997) Brief expression of a GFPcrefusion gene in embryonic stem cells allows rapid retrieval of site-specific genomic deletions (cover-article). Nucleic Acids Res., 25, 3326-3331. PMID: 9241248
  65. Le, Y. and Dobson, M.J. (1997) Stabilization's of yeast artificial chromosome clones in a rad54 recombination-deficient host strain. Nucleic Acid Res. 25, 1248-1253. PMID: 9092636
  66. Le, Y., Smith, K.C., Vining, L.C. and White, R.L. (1996) Mutants ofStreptomyces akiyoshiensis blocked in 5-hydroxy-4-oxonorvaline production. J. Antibiot., 49, 107-109. PMID: 8936306
  67. Le, Y., He, J. and Vining, L.C. (1996) Streptomyces akiyoshiensis differs from other Gram-positive bacteria in the organization of a core biosynthetic pathway gene for aspartate family amino acids. Microbiol.142, 791-798. PMID: 8609075.
  68. Smith, K.C., White, R.L. Le Y., Vining, L.C. (1995) Isolation of N-acetyl-3,4-dihydroxy-L-phenylalanine from Streptomyces akiyoshiensis. J. Nat. Prod.58, 1274-1277.
  69. *Le Y. (1994) Biosynthesis of 5-hydroxy-4-oxonorvaline. Ph.D. Thesis, Dalhousie University, Canada.
  70. *Le, Y, Zhu. S. (1988) Purification of restriction endonuclease MspI. Huaxue Shiji10, (1) 35-37.

*: Le Y as corresponding author.

Methodology Review

1.Le, Y. and Sauer, B. (2001)Conditional gene knockout using Cre recombinase. In Mol Biotechnol. Humana Press, Totowa, NJ. 17(3):269-75. PMID: 11434315.

Books and Book Chapters

  1. Ueki Y., Chollangi S., Le Y., Ash J.D. (2010) gp130 Activation in Müller Cells is not Essential for Photoreceptor Protection from Light Damage. In Retinal Degenerations: Mechanisms and Experimental Therapy. Anderson, R.E. et al. eds. Kluwer Academic/Plenum Publishers (New York): pp 655-661. PMID: 20238070
  2. *LeY., Zheng L., Le, Y. Rucker III E.B., Anderson R.E. (2008) Role of BCL-XL in rod and cone photoreceptor survival. In Retinal Degenerations: Mechanisms and Experimental Therapy. LaVeil. M.M. et al. eds. Kluwer Academic/Plenum Publishers (New York): pp 69-74. PMID: 18188930.
  3. *Le Y., Ash, J.D., Al-Ubaidi, M.R., Chen, Y., Ma, J., and Anderson, R. E. (2006) Conditional knockout system in photoreceptor cells. In Retinal Degenerations: Mechanisms and Experimental Therapy. Hollyfield J. G. et al eds. Kluwer Academic/Plenum Publishers (New York): pp 173-178. PMID: 17249572

*: Le Y. as corresponding author.

Non-authored publications

  1. Magarvey N.A., Demain A.L. (2013) A tribute to Leo C Vining.J Antibiot (Tokyo) 66:359-360.
  2. Clark N.A. (2011) Yun-Zheng Le, Ph.D.: Challenging the outcomes of diabetic retinopathy,Forefront, American Diabetes Association Magazine8 (1):21-22.
  3. Koury C.B. (2007) Genetic system offer option for studying the role of retinal Müller cells-Cover-story-editorial onY. Le’s research program. Retina TodayI (Sept/Oct):31.

National and International Meetings/Abstracts