CURRICULUM VITAE
Personal information:
Name: Guohong Li Place of Birth: Daye, Hubei, China
Date of Birth: Feb. 07, 1972.
Address: National Labortory of Biomacromolecules
Institute of Biophysics, Chinese Academy of Sciences
15 Datun Road, Chaoyang District
Beijing 100101, China
Tel: 0086-10-64888795
Fax: 00086-10-64856269
Email:
Our research is mainly focusing on the biological functions of higher-order chromatin structure, especially the 30nm chromatin fiber in transcriptional regulation of gene. We successfully reconstituted the 30nm chromatin fiber in-vitro using purified system and established a research system to investigate the structural dynamics and in-vitro transcriptional activity of the reconstituted 30nm chromatin fibers. My laboraotry is mainly engaged in the studies on how histone modifications, histone variants and chromatin remodeling complexes are involved in gene activation and silencing via regulating higher order chromatin dynamics. So far, we have published more than 35 research articles in SCI including Science, Cell, Mol Cell, Nature Structural and Molecular Biology, Genes and Development, etc.
Education:
1991-1995: Department of Virology and Molecular Biology, College of Life Sciences,Wuhan University, Hubei, China, Bachelor of Sciences
1995-1998: Department of Biophysics, College of Basic Medicine, Peking University Health Center, Master of Sciences.Thesis: “Structural and functional studies of alpha-actinin ”. Advisor: Prof. Kechun Lin
1998-2003: Max-Planck Institute for Cell Biology/Heidelberg University, Ph.D. magna cum laude in January, 2003. Thesis: “Interaction in vitro of type III intermediate filament proteins with non-B-DNA structures”. Advisor: Prof. Dr. Peter Traub
Postdoctoral training:
2003.6 - 2006.9 Postdoctoral Research Associate, Department of Biochemistry, RWJMS/UMDNJ, Howard Hughes Medical Institute, Piscataway, NJ, Mentor: Danny Reinberg, Ph.D.
2006.9 - 2009.9 Postdoctoral Research Associate, Department of Biochemistry, School of Medicine, NYU, Howard Hughes Medical Institute, New York, NY, Mentor: Danny Reinberg, Ph.D.
Academic Appointments:
2009,9- 2010,2 Research Scientist, Department of Biochemistry, School of Medicine, NYU, New York, NY.
2010.3- “Hundred Talents Program” Investigator, Institute of Biophysics, Chinese Academy of Sciences, Beijing, 100101
Honors, Fellowships，and Awards：
1992-1994 Excellent Student Award, Wuhan University, Hubei, China
1997-1998 Best Graduate Student Award, Peking University Health Center, Beijing, China
1998-2002 International graduate student fellowship, Max-Planck Institute for Cell Biology, Ladenburg/Heidelberg, Germany
2010 “Hundred Talents Program”, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China
Invited Lectures：
Invited Lectures (past five years)：
1. “The Impact of Chromatin Dynamics on RNA pol II Transcription”. Department of Biochemisty, Institute of Basic Medicine, Peking Union Medical University, Beijing, June 2010.
2. “Dynamic Opening of Highly compacted chromatin during RNA pol II Transcription Activation”. Department of Immunology, Third Military Medical University, Chongqing, March 2011.
3. “Higher order chromatin structures and Mechanisms of Epigenetics”. College of Life Sciences, Beijing Normal University, Beijing, March 2011.
4. “Chromatin Plasticity, Histone Variants and Gene Regulation”. College of Life Sciences, Inner Mongolia University of Science & Technology, Baotou, September 2011.
5. “Chromatin Dynamics in Epigenetic Regulation”. College of Life Sciences, Wuhan University, Wuhan, October 2011.
6. “Regulation of Chromatin Dynamics and Transcription by Histone Variants”. Novartis Institutes for BioMedical Research (NIBR), Shanghai, China, August 2012.
7. “The functions of histone variants in chromatin dynamics and gene regulation”. ‘6th Mechanobiology conference: Mechanobiology of Chromatin and Transcription’, Mechanobiology Institute, National University of Singapore, Singapore, November 2012.
8. “Higher order chromatin and epigenetic regulation”. The 7th CNHUPO Annual Congress, Chongqing, China, September 2013.
9. “Regulation of chromatin dynamics and gene transcription by histone variants.” College of Life Science, Fudan University, Shanghai, September 2013.
10. “Regulation of chromatin dynamics and gene transcription by histone variants”. 8th Annual Conference of Asia Epigenome Alliance and 2nd Taipei Epigenetics and Chromatin Meeting, Genomics Research Center, Academia Sinica, Taipei, November 2013.
Publications:
1. Feng Song*, Ping Chen*, Dapeng Sun, Mingzhu Wang, Liping Dong,Dan Liang, Ruiming Xu, Ping Zhu¶ and Guohong Li¶. Cryo-EM study reveals a double helix of the 30 nm chromatin fiber twisted by tetra-nucleosomal units. Science 344(6182), 376-380. (Research article, 2014）
2. Ping Chen*, Yan Wang* and Guohong Li. Dynamics of histone variants H3.3 and its coregulation with H2A.Z at enhancers and promoters. Nucleus 5(1), 1-7. (2014)
3. Ping Chen*, Jicheng Zhao*, Yan Wang*, Haizhen Long, Dan Liang, Haiyong Zhao, Zengqi Wen, Wei Li, Xia Li, Li Huang, Hongli Feng, Ping Zhu, Ming Li, Qainfei Wang, and Guohong Li. Variant histone H3.3 functionally counteracts with H2A.Z to activate gene transcription. Genes and Development, 27(19), 2109-2124. (2013)
4. Gang Yuan, Ben Ma, Wen Yuan, Zhuqiang Zhang, Ping Chen, Xiaojun Ding, Li Feng, Xiaohua Shen, She Chen, Guohong Li and Bing Zhu. H2A ubiquitination inhibits the enzymatic activity of H3K36 methyltransferases. J. Biol. Chem., 288, 30832-30842. (2013)
5. Ping Chen, Jicheng Zhao, and Guohong Li. Histone variants in development and diseases. J Genet Genomics. 40(7), 355-365. (2013)
6. Min-Xian Qian, Dan-Yang Ji, Ye Pang, Cui-Hua Liu, Kousuke Haratake, Bo-Yu Du, Guang-Fei Wang, Qian-Qian Zhu, Wei Song, Yadong Yu, Xiao-Xu Zhang, Hai-Tao Huang, Shiying Miao, Lian-Bin Chen, Zi-Hui Zhang, Ya-Nan Liang, Shan Liu, Hwangho Cha, Dong Yang, Yonggong Zhai, Takuo Komatsu, Fuminori Tsuruta, Haitao Li, Cheng Cao, Wei Li, Guo-Hong Li, Yifan Cheng, Tomoki Chiba, Linfang Wang, Alfred L. Goldberg, Yan Shen, and Xiao-Bo Qiu. Acetylation-Mediated Degradation of Core Histones by PA200/Blm10-Containing Proteasomes. Cell,153(5):1012-1024.(2013)
7. Philipp Tropberger, Sebastian Pott, Claudia Keller, Kinga Kamieniarz-Gdula, Matthieu Caron, Florian Richter, Guohong Li, Gerhard Mittler, Edison T. Liu, Marc Bühler, Raphael Margueron, and Robert Schneider. Regulation of Transcription through Acetylation of H3K122 on the Lateral Surface of the Histone Octamer. Cell, 152(4), 859-872. (2013)
8. Chao-pei Liu*, Chaoyang Xiong*, Mingzhu Wang, Zhouliang Yu, Na Yang, Ping Chen, Zhiguo Zhang, Guohong Li and Rui-Ming Xu. Structure of the variant histone H3.3-H4 heterodimer in complex with its chaperone DAXX. Nature Structural and Molecular Biology 19(12)，1287-1292. (2012)
9. Yu Qiu, Lei Liu, Chen Zhao, Chuanchun Han, Fudong Li, Jiahai Zhang, Yan Wang, Guohong Li, Yide Mei, Mian Wu, Jihui Wu, and Yunyu Shi. Combinatorial readout of unmodified H3R2 and acetylated H3K14 by the tandem PHD finger of MOZ reveals a regulatory mechanism for HOXA9 transcription. Genes and Development 26(12), 1376-1391. (2012)
10. YuQiu, WenZhang, ChenZhao, YanWang, WeiweiWang, JiahaiZhang, ZhiyongZhang, GuohongLi, YunyuShi, XiaomingTu and JihuiWu. Solution structure of Pdp1 PWWP domain reveals its unique binding sites for methylated H4K20 and DNA. Biochem J. 442(3), 527-38.（2012）
11. ChengkunWang, JieShen, ZhongzhengYang, PingChen, BinZhao, WeiHu, WenxianLan, XiaotianTong, HoumingWu, GuohongLi and ChunyangCao. Structural basis for site-specific readout of unmodified R2 of histone H3 tail by UHRF1 PHD finger. Cell Res. 21, 1379-1382. (2011)
12. Bin-ZhongLi, ZhengHuang, Qing-YanCui, Xue-HuiSong, LinDu, AlbertJeltsch, PingChen, GuohongLi, EnLi and Guo-LiangXu. Histone tails regulate DNA methylation by allosterically activating de novo methyltransferase. Cell Res. 21, 1172-1181. (2011)
13. Beck DB, Bonasio R, Kaneko S, Li G, Li G, Margueron R, Oda H, Sarma K, Sims RJ 3rd, Son J, Trojer P, Reinberg D. Chromatin in the nuclear landscape. Cold Spring Harb Symp Quant Biol.75,11-22. (2011)
14. Patrick Trojer, Alina R. Cao, Zhonghua Gao, Yan Li, Jin Zhang, Xiaoqin Xu, Guohong Li, Regine Losson, Hediye Erdjument-Bromage, Paul Tempst, Peggy J. Farnham and Danny Reinberg. L3MBTL2 protein acts in concert with PcG protein mediated monoubiquitination of H2A to establish a repressive chromatin structure. Mol. Cell 42, 438-450. (2011).
15. Hao Hu, Yang Liu1, Mingzhu Wang, Junnan Fang, Hongda Huang, Na Yang, Yanbo Li, Jianyu Wang, Xuebiao Yao, Yunyu Shi, Guohong Li and Rui-Ming Xu. Structure of a CENP-A-Histone H4 Heterodimer in Complex with Chaperone HJURP. Genes and Development 25, 901-906. (2011).
16. Guohong Li and Danny Reinberg. Chromatin higher-order structures and gene regulation. Curr Opin Genet Dev. 21, 175-186. (2011)
17. Ping Chen and Guohong Li. Dynamics of the higher-order structure of chromatin. Protein Cell. 1, 967-971. (2010).
18. Eric I. Campos, Jeffrey Fillingham*, Guohong Li*, Haiyan Zheng*, Philipp Voigt, Wei-Hung W. Kuo, Harshika Seepany, Zhonghua Gao, Loren A. Day, Jack F. Greenblatt and Danny Reinberg. The Program for Processing Replicative Histones H3.1 and H4: from the Cytoplasm to the Nucleus. Nature Structural and Molecular Biology 17, 1343-1351. (2010) (*Equal contribution)
19. Guohong Li; Raphael Margueron; Guobin Hu; David Stokes; Yuh-Hwa Wang; Danny Reinberg. Highly Compacted Chromatin Formed in vitro Reflect the Dynamics of Transcription Activation in vivo. Mol. Cell, 38, 41-53. (2010)
20. Schutte B. Henfling M.E.R., Verheyen F.K.C.P., Li G., Tolstonog G. and Ramaekers F.C.S. The caspase-9 derived C-terminal fragment of cytokeratin 18 modulates topoisomerase action. Int. J. of Oncology 35, 625-630. (2009)
21. Raphael Margueron; Guohong Li; Kavitha Sarma; Alexandre Blais; Jiri Zivadil; Brian Dynlacht; Danny Reinberg. Ezh1 and Ezh2 maintain repressive chromatin through different mechanisms. Mol Cell 32, 503-518. (2008)
22. Patrick Trojer*, Guohong Li*, Robert J. III Sims*, Alejandro Vaquero, Nagesh Kalakonda, Piernicola Boccuni, Donghoon Lee, Hediye Erdjument-Bromage, Paul Tempst, Stephen D. Nimer, Yuh-Hwa Wang, Danny Reinberg. L3MBTL1, a histone-methylation-dependent chromatin lock. Cell 129, 915-928. (2007) (*Equal contribution)
23. Robert J. III Sims*,Patrick Trojer*, Guohong Li, Danny Reinberg. Methods to identify and functionally analyze factors that specifically recognize histone lysine methylation. Methods 40, 331-338. (2006) (*Equal contribution)
24. Rushad Pavri, Bing Zhu, Guohong Li, Patrick Trojer, Subhrangsu Mandal, Ali Shilatifard and Danny Reinberg. Histone H2B Monoubiquitination Functions Cooperatively with FACT to Regulate Elongation by RNA Polymerase II. Cell 125, 703-717. (2006)
25. Genrich V. Tolstonog, Guohong Li, Robert L. Shoeman, and Peter Traub. Interaction in vitro of type III intermediate filament proteins with higher order structures of single-stranded DNA, particularly with G-quadruplex DNA. DNA and Cell Biology 24, 85-110. (2005)
26. Guohong Li, Genrich V. Tolstonog, Michael Sabasch and Peter Traub. Type III intermediate filament proteins interact with four-way junction DNA and facilitate its cleavage by the junction-resolving enzyme T7 endonuclease I. DNA and Cell Biology 22, 261-291. (2003)
27. Guohong Li, Genrich V. Tolstonog and Peter Traub. Interaction in vitro of type III intermediate filament proteins with Z-DNA and B-Z-DNA junctions. DNA and Cell Biology 22, 141-169. (2003) (Cover story)
28. Guohong Li, Genrich V. Tolstonog and Peter Traub. Interaction in vitro of type III intermediate filament proteins with triplex DNA. DNA and Cell Biology 21, 163-188. (2002)
29. Guohong Li, Genrich V. Tolstonog, Michael Sabasch and Peter Traub. Interaction in vitro of type III intermediate filament proteins with supercoiled plasmid DNA and modulation of eukaryotic DNA topoisomerase I and II activities. DNA and Cell Biology 21, 743-769. (2002) (Cover story)
30. Guohong Li, Gang Li, Yang Qiu and Kechun Lin. pH and sodium cation induced conformational changes of a-actinin: a fourier transform infrared spectroscopic study. Acta Biophysica Sinica 15, 283-288. (1999)
31. Guohong Li, Gang Li, Yang Qiu and Kechun Lin. Conformational change and interaction of a-actinin with palmitic acid and diacrylglycerol. Acta Biophysica Sinica 15, 463-469. (1999)
32. Duncheng Wang, Fengrong Bu, Guohong Li, Gang Li and Kechun Lin. The primary analysis for the effects of MB/photochemical treatment on the FT-IR spectra and activity of human Ig G. Acta Biophysica Sinica 15, 156-171. (1999)
33. Xing Han, Guohong Li, Gang Li and Kechun Lin. FTIR study of the thermal denaturation of a-actinin in lipid-free and DOPG-bound states and the central and N-terminal domains of a-actinin in D2O. Biochemistry 37, 10730-10737. (1998)
34. Xing Han, Gang Li, Guohong Li and Kechun Lin. Interactions between smooth muscle a-actinin and lipid bilayers. Biochemistry 36, 10364-10371. (1997)
35. Yanhua Du, Guanying Qiu, Guohong Li, Yunzi Liu, Haibiao Du and Yifong Yin. Studies on change of infective rate of PxGV and analysis of dose-effect curves induced by low-energy ion beam and g-rays. Acta Biophysica Sinica 13, 267-272. (1997)
36. Xing Han, Guohong Li, Gang Li, Kechun Lin, Xing Zhang and Wei Xu. Interactions of a-actinin amd membranes studied by the methods of fluorescence energy transfer and electron microscopy. Acta Biophysica Sinica 13, 40-44. (1997)