Name : Dr. Srigiridhar Kotamraju
Designation : Senior Scientist
Male / Female : Male
Address : Centre for Chemical Biology, CSIR-Indian Institute of Chemical
Technology (IICT), Tarnaka, Hyderabad-500007
Date of birth : 10-12-1970
E-mail address :
Phone No. : 040-27191867; 9908213979
Educational Qualifications :
Degrees obtained (Begin with Bachelor's Degree)
______
Degree Institution Field/Subject(s) Year
______
1). B.Sc. Osmania University Microbiology,Zoology,Chemistry. 1991
2). M.Sc. University of Hyderabad Life Sciences 1993
3). PhD Osmania University Biochemistry 1999
Research /Training Experience:
Sl No. / InstitutionPlace / Position / From (Date) / To (date)
1 / Indian Institute of Chemical Technology / Senior Scientist / July 15th 2011 / Till date
2 / Indian Institute of Chemical Technology / Ramanujan Fellow / May 1st 2008 / July 14th 2011
3 / Institute of Life Sciences, Hyderabad / Senior Research Scientist / March 1st 2007 / April 30th 2008
4 / Medical College of Wisconsin, Milwaukee, WI, USA / Senior Research Scientist / Feb 2004 / Jan 2007
5 / Medical College of Wisconsin, Milwaukee, WI, USA / Research Scientist / Feb 2003 / Jan 2004
6 / Medical Coll. of Wisconsin, Milwaukee, WI, USA / Post doctoral fellow / Jan 1999 / Jan 2003
Research specialization achieved
1. Molecular and signaling mechanisms of vascular diseases, oxidative stress,
inflammation and mitochondrial therapeutics.
2. Breast cancer and metastasis;
3. small molecules screening against disease targets.
peer-reviewed publications: 63
1. Srigiridhar, K., Nair, K.M. Protective effects of antioxidant enzymes and GSH in vivo in gastrointestinal tract of rat during iron supplementation. Ind. J. Biochem. Biophys. 34:402-406, 1997.
2. Srigiridhar, K., Nair, K.M. Iron deficient intestine is more susceptible to peroxidative damage during iron supplementation in rats. Free Radic. Biol. Med. 25:660-5, 1998.
3. Srigiridhar, K., Nair, K.M. Supplementation with alpha-tocopherol or a combination of alpha-tocopherol and ascorbic acid protects the gastrointestinal tract of iron-deficient rats against iron-induced oxidative damage during iron repletion. Br. J. Nutr. 84:165-73, 2000.
4. Kotamraju, S., Konorev, E.A., Joseph, J., Kalyanaraman, B. Doxorubicin-induced apoptosis in endothelial cells and cardiomyocytes is ameliorated by nitrone spin traps and ebselen. Role of reactive oxygen and nitrogen species. J. Biol. Chem. 275:33585-33592, 2000.
5. Srigiridhar, K., Nair, K.M., Subramanian, R., Singotamu, L. Oral repletion of iron induces free radical mediated alterations in the gastrointestinal tract of rat. Mol. Cell. Biochem. 219:91-8, 2001.
6. Kotamraju, S., Hogg, N., Joseph, J., Keefer, L.K., Kalyanaraman, B. Inhibition of oxidized low-density lipoprotein-induced apoptosis in endothelial cells by nitric oxide: peroxyl radical as an antiapoptotic mechanism. J. Biol. Chem. 276:17316-23, 2001.
7. Kalivendi, S.V., Kotamraju, S., Zhao, H., Joseph, J., Kalyanaraman, B. Doxorubicin-induced apoptosis is associated with increased transcription of endothelial nitric-oxide synthase: effect of antiapoptotic antioxidants and calcium. J. Biol. Chem. 276: 47266-47276, 2001.
8. Kotamraju, S., Chitambar, C.R., Kalivendi, S.V., Joseph, J., Kalyanaraman, B. Transferrin receptor-dependent iron uptake is responsible for doxorubicin-mediated apoptosis in endothelial cells. J. Biol. Chem. 277: 17179-17187, 2002.
9. Konorev, E., Kotamraju, S., Zhao, H., Kalivendi, S., Joseph, J., and Kalyanaraman, B.: Paradoxical effects of metalloporphyrins on doxorubicin-induced apoptosis: scavenging of reactive oxygen species versus induction of heme oxygenase-1. Free Radic. Biol. Med. 33, 988-997 (2002)
10. Newman, D. K., Hoffman, S., Kotamraju, S., Zhao, T., Wakim, B., Kalyanaraman, B., Newman, P. J.: Nitration of PECAM-1 ITIM tyrosines abrogates phosphorylation and SHP-2 binding. Biochem. Biophys. Res. Commun. 296, 1171-9 (2002).
11. Wang, S., Kotamraju, S., Konorev, E., Kalivendi, S., Joseph, J., Kalyanaraman, B.: Activation of nuclear factor-kappaB during doxorubicin-induced apoptosis in endothelial cells and myocytes is pro-apoptotic: the role of hydrogen peroxide. Biochem. J. 367, 729-740 (2002).
12. Tampo, Y., Kotamraju,S., Chitambar, C.R., Kalivendi, S.V., Keszler, A., Joseph, J., Kalyanaraman, B.: Oxidative stress-induced iron signaling is responsible for peroxide-depended oxidation of dichlorodihydrofluorescein in endothelial cells. Circ. Res. 92, 56-63 (2003).
13. Kalivendi, S. V., Kotamraju, S., Cunningham, S., Shang, T., Hillard, C. J., Kalyanaraman, B.: 1-Methyl-4-phenylpyridinium (MPP+)-induced apoptosis and mitochondrial oxidant generation: role of transferrin receptor dependent iron and hydrogen peroxide. Biochem. J. 371, 151-164 (2003).
14. Kotamraju, S., Tampo, Y., Keszler, A., Chitambar, C.R., Joseph, J., Haas, A.L., Kalyanaraman, B. Nitric oxide inhibits H2O2-induced transferrin receptor-dependent apoptosis in endothelial cells: Role of ubiquitin-proteasome pathway. Proc. Natl. Acad. Sci. USA 100 (19), 10653-8 (2003).
15. Maas, M., Wang, R., Paddock, C., Kotamraju, S., Kalyanaraman, B., Newman, P.J., Newman, D.K. Reactive oxygen species induce reversible PECAM-1 tyrosine phosphorylation and SHP-2 binding. Am. J. Physiol. Heart Circ. Physiol. 285, H2336-H2344 (2003).
16. Shang, T., Kotamraju, S., Kalivendi, S.V., Hillard, C.J., Kalyanaraman, B. 1-Methyl-4-phenylpyridinium-induced apoptosis in cerebellar granule neurons is mediated by transferrin receptor iron-dependent depletion of tetrahydrobiopterin and neuronal nitric oxide synthase-derived superoxide. J. Biol. Chem. 279, 19099-19112 (2004).
17. Kalivendi SV, Cunningham S. Kotamraju S, Joseph J, Hillard CJ, Kalyanaraman B. Alpha-synuclein up-regulation and aggregation during MPP+-induced apoptosis in neuroblastoma cells: Intermediacy of transferrin receptor iron and hydrogen peroxide. J. Biol. Chem. 279, 15240-15247 (2004).
18. Wang, S., Konorev, E.A., Kotamraju, S., Joseph, J., Kalivendi, S., Kalyanaraman, B. Doxorubicin induces apoptosis in normal and tumor cells via distinctly different mechanisms: Intermediacy of H2O2- and p53-dependent pathways. J. Biol. Chem. 279, 25535-25543 (2004).
19. Matsunaga, T., Kotamraju, S., Kalivendi, S.V., Dhanasekaran, A., Joseph, J., Kalyanaraman, B. Ceramide-induced intracellular oxidant formation, iron signaling, and apoptosis in endothelial cells: protective role of endogenous nitric oxide. J. Biol. Chem. 279, 28614-28624 (2004).
20. Dhanasekaran, A., Kotamraju, S., Kalivendi, S.V., Matsunaga, T., Shang, T., Keszler, A., Joseph, J., Kalyanaraman, B. Ceramide-induced intracellular oxidant formation, iron signaling, and apoptosis in endothelial cells: protective role of endogenous nitric oxide. J. Biol. Chem. 279, 37575-37587 (2004).
21. Dhanasekaran, A., Kotamraju, S., Karunakaran, C., Kalivendi, S. V., Thomas, S., Joseph, J., Kalyanaraman, B. Mitochondria superoxide dismutase mimetic inhibits peroxide-induced oxidative damage and apoptosis: Role of mitochondrial superoxide. Free Radic. Biol. Med. 39, 567-583 (2005).
22. Shang, T., Kotamraju, S., Zhao, H., Kalivendi, S.V., Hillard, C.J., Kalyanaraman, B. Sepiapterin attenuates 1-methyl-4-phenylpyridinium-induced apoptosis in neuroblastoma cells transfected with neuronal NOS: Role of tetrahydrobiopterin, nitric oxide and proteasome activation. Free Radic. Biol. Med. 39, 1059-1074 (2005).
23. Kotamraju, S., Matalon, S., Matsunaga, T., Shang, T., Hickman-Davis, J. M., Kalyanaraman, B. Upregulation of immunoproteasomes by nitric oxide: potential antioxidative mechanism in endothelial cells. Free Radic. Biol. Med. 40, 1034-1044 (2006).
24. Chitambar, CR., Kotamraju, S., Werely, JP. Expression of the hemochromatosis gene (HFE) modulates the cytotoxicity of doxorubicin in breast cancer cells. Int. J. Cancer 119, 2200-4 (2006).
25. Thomas, S., Kotamraju, S., Zielonka, J., Harder DR., Kalyanaraman, B. Hydrogen peroxide induces nitric oxide and proteosome activity in endothelial cells: A bell-shaped signaling response. Free Radic. Biol. Med. 42, 1049-61 (2007).
26. Kotamraju, S., Williams, C., Kalyanaraman, B. Statin-induced breast cancer cell death: Role of inducible
nitric oxide and arginine metabolizing enzymes. Cancer Research 67. 7386-94 (2007).
27. Chandrasekhar, S., Basu, D., Sailu, M., Kotamraju S. Novel synthetic route to the tricyclic core of (+/-)-galanthamine. Tetrahedron Letters 50. 4882-84 (2009).
28. Rajesh, S., Arivudainambi USE., Raja Singh, S., Rajendran, A., Kotamraju S., Karunakaran, C. Development of superoxide anion radical biosensor using self-assembled cysteine monolayer on gold particles in polypyrrole matrix facilitated electron transfer in Cu,ZnSOD. Sensor Letters 8: 1-9 (2010).
29. Lakshminarayana Reddy, CN., Vyjayanti, VN., Notani, D., Galande, S., Kotamraju, S. Down regulation of global gene regulator SATB1 by statins in COLO205 colon cancer cells. Mol. Med. Rep. 3: 857-861 (2010).
30. Rajesh S, Kanugula AK, Bhargava K, Ilavazhagan G, Kotamraju S, Karunakaran C. Simultaneous electrochemical determination of superoxide anion radical and nitrite using Cu,ZnSOD immobilized on carbon nanotube in polypyrrole matrix. Biosens Bioelectron. 26: 689-695 (2010).
31. Kurumurthy C, Sambasiva Rao P, Veeraswamy B, Santhosh Kumar G, Shanthan Rao P, Sathish BV,
Choudhury C, Kotamraju S, Narasaiah B. A facile and single pot strategy for the sythesis of novel napthyridine
derivativs inder microwave irradiation conditions using ZnCl2 as a catalyst, evaluation of AChE inhibitory
activity and molecular modeling studies. Medicinal Chemistry Research. 21: 1785-95, 2012.
32. Khatua TN, Padiya R, Karnewar S, Kuncha M, Agawane SB, Kotamraju S, Banerjee SK.Garlic
provides protection to mice heart against isoproterenol-induced oxidative damage: Role of nitric
oxide. Nitric Oxide 27: 9-17, 2012.
33. Thangamuthu Madasamy, ManickamPandiaraj, MurugesanBalamurugan , SantoshKarnewar, Alby
RobsonBenjamin, KrishnaArunVenkatesh, Kanagavel Vairamani, Kotamraju S , Chandran
Karunakaran. Virtual electrochemical nitric oxide analyzer using copper, zinc superoxide dismutase
immobilized on carbon nanotubes in polypyrrole matrix. Talanta 100: 168–174, 2012
34. Pandiaraj M, Madasamy T, Gollavilli PN, Balamurugan M, Kotamraju S, Rao VK, Bhargava K, Karunakaran
C. Nanomaterial-based electrochemical biosensors for cytochrome c using cytochrome c reductase.
Bioelectrochemistry 91C:1-7, 2012.
35. Lakshmi SV, Naushad SM, Reddy CA, Saumya K, Rao DS, Kotamraju S, Kutala VK. Oxidative stress in
coronary artery disease: Epigenetic perspective. Mol Cell Biochem. 374: 203-11, 2013.
36. Veeraswamy B, Kurumurthy C, Santhosh Kumar G, Sambasiva Rao P, Kavya Thelakkat, Kotamraju S &
Narsaiah B. Synthesis of novel 5-substituted isoxazole-3-carboxamide derivatives and cytotoxicity studies on
lung cancer cell line. Indian J. Chem. 51B: 1365-68, 2012.
37. Sambasiva Rao P, Kurumurthy C Veeraswamy B Santhosh kumar G, Narsaiah B, Pranay Kumar K,
Murthy USN, Karnewar S, Kotamraju S. Synthesis, antimicrobial and cytotoxic activities of novel 4-
trifluoromethyl-(1,2,3)-thiadiazolo-5-carboxylic acid hydrazide Schiff’s bases. Med. Chem. Res.
22: 1747-55, 2013.
38. Koppireddi S, Komsani JR, Avula S, Pombala S, Vasamsetti S, Kotamraju S, Yadla R. Novel 2-(2,4-
dioxo-1,3-thiazolidin-5-yl)acetamides as antioxidant and/or anti-inflammatory compounds. Eur. J.
Med. Chem. 66:305-13, 2013.
39. Naushad SM, Reddy CA, Kumaraswami K, Divyya S, Kotamraju S, Gottumukkala SR, Digumarti and
RR, Kutala VK. Impact of Hyperhomocysteinemia on Breast Cancer Initiation and Progression:
Epigenetic Perspective. Cell Biochem Biophys. 68:397-406, 2013.
40. Kamal A, Reddy VS, Karnewar S, Chourasiya SS, Shaik AB, Kumar GB, Kishor C, Reddy MK,
Narasimha Rao MP, Nagabhushana A, Ramakrishna KV, Addlagatta A, Kotamraju S. Synthesis and
Biological Evaluation of Imidazopyridine-Oxindole Conjugates as Microtubule-Targeting Agents.
ChemMedChem. 8(12):2015-25, 2013.
41. Reddy GM, Vasamsetti S, Nagender P, Kumar RN, Kurumurthy C, Rao PS, Kotamraju S, Narsaiah
B. Synthesis of novel benzothiazine-1,1-dioxo-3-yl-1,2,3-triazol-4-yl prop 2-en-1-one derivatives and
their evaluation for IL-1b, TNF-a and IL-8. Eur. J. Med. Chem. 75:143-50, 2013.
42. Madasamy T, Pandiaraj, Kanugula AK, Rajesh S, Bhargava K , Sethy NK, Kotamraju S, and Karunakaran C,
Gold Nanoparticles with Self-Assembled Cysteine Monolayer Coupled to Nitrate Reductase in Polypyrrole
Matrix Enhanced Nitrate Biosensor. Adv. Chem. Lett. 1: 2-9, 2013.
43. Reddy CA, Somepalli V, Golakoti T, Kanugula AK, Karnewar S, Rajendiran K, Vasagiri N,
Prabhakar S, Kuppusamy P, Kotamraju S and Kutala VK. Mitochondrial-targeted curcuminoids: a
strategy to enhance bioavailability and anticancer efficacy of curcumin. PLoS One. 12;9(3):e89351,
2014.
44. Sambasiva Rao P, Kurumurthy C, Veeraswamy B, Santhosh Kumar G, Poornachandra Y, Ganesh
Kumar C, Vasamsetti SB, Kotamraju S and Narsaiah B. Synthesis of novel 1,2,3-triazole substituted-N-
alkyl/aryl nitrone derivatives, their anti-inflammatory and anticancer activity. Eur J Med Chem.
80C:184-191, 2014.
45. Kanugula AK, Gollavilli PN, Vasamsetti S, Karnewar S, Gopoju R; Ummanni R, Kotamraju S.
Statin-induced inhibition of breast cancer proliferation and invasion involves attenuation of iron
transport: Intermediacy of nitric oxide and antioxidant defence mechanisms. FEBS J. 281: 3719–3738,
2014.
46. Kanugula AK, Dhople VM, Völker U, Ummanni R, Kotamraju S. Fluvastatin mediated breast
Cancer cell death: A proteomic approach to identify the differentially regulated proteins in MDA-
MB-231 cells. PLoS ONE 9(9): e108890, 2014.
47. Kamal A, Vangala SR, Karnewar S, Kumar GB, Shaik AB, Mahesh R, Chourasiya, Sayeed IB
and Kotamraju S. MedChemComm., 1718-1723, 2014.
48. Kanugula AK and Kotamraju S. Statin-induced molecular mechanisms of breast cancer cell death:
Recent developments. Can. Cell Microenviron. 1: e390 (2014).
49. Koppireddi S, Chilaka DR, Avula S, Komsani JR, Kotamraju S and Yadla R. Synthesis and anticancer
evaluation of 3-aryl-6-phenylimidazo[2,1-b]thiazoles. Bioorg Med Chem Lett. 2014
20;24(23):5428-5431
50. Kumar NR, Reddy GM, Nagendar P, Kurumurthy C, Shanthan Rao P, Karnewar S, Kotamraju S and Narsaiah
B. Synthesis of Novel Pyrido[3′,2′:4,5]furo[3,2-d]pyrimidine Derivatives and Their Cytotoxic Activity. J.
Heterocyclic Chem. 51: 1531-1535, 2014.
51. Raju K, Chandra Shekhar A, Sathaiah G , Ravi Kumar A , Narsaiah B , Shanthan Rao P, Srujana R and
Kotamraju S. Synthesis, Characterization and Antitumor Activity of Novel Triazole/Isoxazole Tagged Pyridine
Hybrids. Letters in Organic Chemistry 11: 1-11, 2014.
52. Vasamsetti S, Karnewar S, Kanugula AK, Avinash TR, Jerald MK, Kotamraju S. Metformin inhibits
monocyte-to-macrophage differentiation via AMPK mediated-inhibition of STAT3 activation:
Potential role in atherosclerosis. Diabetes. 64: 2028-2041, 2015.
53. Gannarapu MR, Vasamsetti SB, Punna N, Kotamraju S and Narsaiah B. Synthesis of novel 1-substituted triazol
linked 1,2-benzothiazine 1,1-dioxido propenone derivatives as potent anti-inflammatory agents and inhibitors of
monocyte-to-macrophage differentiation. Med. Chem. Commun., 6, 1494-1500, 2015.
54. Gollavilli PN, Kanugula AK, Koyyada R, Karnewar S, Neeli PK, Kotamraju S. AMPK inhibits MTDH
expression via GSK3β and SIRT1 activation: potential role in triple negative breast cancer cell proliferation.
FEBS J. doi: 10.1111/febs.13391, (2015).
55. Sajja Y, Vulupala HR, Bantu R, Nagarapu L, Vasamsetti SB, Kotamraju S, Nanubolu JB. Three-component, one-pot synthesis of benzo[6,7]cyclohepta[1,2-b]pyridine derivatives under catalyst free conditions and evaluation of their anti-inflammatory activity. Bioorg Med Chem Lett. 26:858-63, (2016).
56. Ramesh V, Santosh K, Anand TD, Shanmugaiah V, Kotamraju S, Karunakaran C, Rajendran A. Novel Bioactive Wild Medicinal Mushroom-Xylaria sp. R006 (Ascomycetes) against Multidrug Resistant Human Bacterial Pathogens and Human Cancer Cell Lines. Int J Med Mushrooms. 17:1005-17, (2015).
57. Karnewar S, Vasamsetti SB, Kanugula AK, Gopoju R, Ganji SK, Prabhakar S, Rangaraj N, Kumar JM and Kotamraju S. Mitochondria-targeted esculetin alleviates mitochondrial dysfunction by AMPKα1 mediated nitric oxide and SIRT3 regulation in endothelial cells: potential implications in atherosclerosis. Sci. Rep. 6: 24108, (2016).