Nadine LUSAMBA, BPharm, MSc.

78-56 Goodview Rd, North York – ON M2J2K6 (647) 702- 8044

HIGHLIGHTS OF QUALIFICATIONS:

Bilingual (English, French) scientific professional holding a degree of

•Master of Science in Molecular pharmacology, with 5 years of experience in Laboratory management and technics, data analysis, scientific publication and presentation. Demonstrated ability to work on multiple project under deadline, strong individual contributor and collaborative team member.

•Clinical study, drug safety and pharmacovigilance Diploma in process

RELEVANT SKILLS & EXPERIENCE:

Experience in writing scientific publications and presenting at scientific meetings and conferences (list of publications included)

Demonstrated strong knowledge of drug effect and intracellular mechanism

  • Strong background in Rhinovirus and influenza virus experiments,
  • Good command on laboratory’s techniques and methods include Elisa, Western blot, MTT assay, Trypan blue, RNA purification, Cytokines quantification, endosome identification and quantification, RT-qPCR, Isolation and quantification of NFk-B proteins, laboratory materials sterilization,
  • Proven skills in human cells culture, cells growth, Cell viability, cells counting, cells infection by virus and treatment by drug.

Acknowledged for highly organized, detail oriented, exemplary work ethic resulting in high quality of data and research outcome.

Recognized by management for scientific curiosity, hard-working, team-oriented approach, , and demonstrated learning abilities to adopt new methodologies consistently and quickly.

Mentored and trained new employees and students in laboratory techniques and data analysis methods

Advanced command of Softmax-pro 6 software and statistical software as well as of Word, Excel and PowerPoint.

PROFESSIONAL WORK EXPERIENCE:

Research Assistant, Department of Advanced Preventive Medicine for Infectious Disease, Tohoku University, Sendai, Japan 2011-2016

•Planned and executed laboratory experiments, including protocol preparation, reagents order, Cell culture medium preparation, Cell culture, Virus titration and Cell infection

•Performed In vitro experiments on human cells to evaluate effect of drug on virus infection, and drug intracellular mechanism

•Management of laboratory materials includes ordering laboratory supplies (micro-tubes, pipettes, plates) and disposal of laboratory wastes.

•Data analysis, writing articles for publication and presentation at scientific meetings.

Teaching Assistant, Department of Molecular Pharmacology, Tohoku University 2014-2015.

•Support teaching and evaluation of undergraduate students at Tohoku University.

Pharmacist, PharmabelApoteck 2007-2009.

•Dispensing medicine as per medical prescription

•Advising patient about the use of over counter drugs

•Ensuring the management of the drug store, (procurement, storage, inventory)

EDUCATION:

Clinical Research, Drug Safety and Pharmacovigilance Post Graduate Diploma

in process: Academy of Applied Pharmaceutical Sciences, Toronto, Ontario 2017-2018

Certificate of:

•NIH Plain Language

•TCPS 2: Tri-Council Policy Statement: Ethical Conduct for Research Involving Humans

•The Canadian Pharmaceutical Industry: Big Picture

•Introduction to Clinical Research and Drug Safety

•Legislations, Guidelines and Ethics in Clinical Research and Drug Safety

•Clinical Pharmacology and Clinical Safety Assessments

•Clinical Research Regulations and Good Clinical Practices (GCP)

•Medical Devices

•Organization of Clinical Trials and Clinical Monitoring Plan Development

•Clinical Data Acquisition, Data Management and Biostatistics

•Clinical Research SOP Development

•Clinical Study and safety Reporting Activities

•Global Clinical Research and Pharmacovigilance

•Drug Lifecycle Safety Management and Pharmacovigilance Compliance In progress

•Pharmaceutical Biotechnology In progress

•Clinical Study Protocol and Investigators’ Brochure Development In progress

Master of Science Diploma in Molecular pharmacology: Graduate School of Medicine, Tohoku University in Japan. 2015.

Bachelor in pharmacy: University of Kinshasa, School of Pharmacy, Pharmacist Diploma 2006.

MEMBERSHIP IN PROFESSIONAL ASSOCIATIONS:

•Japanese Pharmacological Society (JPS): member number: nq144RU01

•Ordre des pharmaciens du Congo: member number 1068/05

Presentations in conferences:

1.Oral: Nadine Lusamba Kalonji, MutsuoYamaya, Hiroshi kubo, Chiharu Ota, Sato Takeya, Yanagisawa Teruyuki. Oral presentation: “Inhibitory effect of Novel Macrolide non-antibiotic EM900 on Rhinovirus infection”. At the 65th annual meeting of North Japanese Pharmacological Society, Fukushima 26-27 September 2014.

2.PosterHoda Bagheri, kimshunketsu, Nadine Lusamba Kalonji, Satō Takeya, Yanagisawa Teruyuki, Sukegawa Jun. Poster: “Analysis of Rab proteins interacting with histamine H3 receptor”. At the 87th Annual meeting of Japanese Pharmacological Society, Sendai-shi.19-21 March 2013

PUBLICATIONS [1-9]:

1MutsuoYamaya, Kazuhiro Nomura, Kazuya Arakawa, Hidekazu Nishimura, Nadine Lusamba Kalonji, Hiroshi Kubo, Ryoichi Nagatomi, TetsuakiKawase:

“Increased rhinovirus replication in nasal mucosa cells in allergic subjects is associated with increased ICAM-1 levels and endosomal acidification and is inhibited by L-carbocisteine” Immunity, Inflammation and Disease Volume 4, Issue 2, pages 166–181, June 2016 15 APR 2016 DOI: 10.1002/iid3.102

2Lusamba Kalonji Nadine, Nomura K, Kawase T, Ota C, Kubo H, Sato T, Yanagisawa T, Sunazuka T, Omura S, Yamaya M: “The non-antibiotic macrolide EM900 inhibits rhinovirus infection and cytokine production in human airway epithelial cells”. Physiological reports 2015, 3(10).

3Yamaya M, Nadine Lusamba, Kubo H, Saito K, Saito R, Nishimura H: “The effects of neuraminidase inhibitors on the release of oseltamivir-sensitive and oseltamivir-resistant influenza viruses from primary cultures of human tracheal epithelium”. Journal of medical virology 2015, 87(1):25-34.

4Yamaya M, Shimotai Y, Hatachi Y, Lusamba Kalonji N, Tando Y, Kitajima Y, Matsuo K, Kubo H, Nagatomi R, Hongo S et al: “The serine protease inhibitor camostat inhibits influenza virus replication and cytokine production in primary cultures of human tracheal epithelial cells”. Pulmonary pharmacology & therapeutics 2015, 33:66-74.

5Yamaya M, Nadine Lusamba Kalonji, Ota C, Kubo H, Makiguchi T, Nagatomi R, Nishimura H: “Magnitude of influenza virus replication and cell damage is associated with interleukin-6 production in primary cultures of human tracheal epithelium”. Respiratory physiology & neurobiology 2014, 202:16-23.

6Yamaya M, Nishimura H, Nadine Lusamba, Kubo H, Nagatomi R: “Formoterol and budesonide inhibit rhinovirus infection and cytokine production in primary cultures of human tracheal epithelial cells”. Respiratory investigation 2014, 52(4):251-260.

7Yamaya M, Nishimura H, Nadine Lusamba Kalonji, Ota C, Kubo H, Nagatomi R: “Ambroxol inhibits rhinovirus infection in primary cultures of human tracheal epithelial cells”. Archives of pharmacal research 2014, 37(4):520-529.

8Yamaya M, Nishimura H, Nadine Lusamba, Kubo H, Ryoichi N: “Tulobuterol inhibits rhinovirus infection in primary cultures of human tracheal epithelial cells”. Physiological reports 2013, 1(3):e00041.

9MutsuoYamaya , M.D., Ph.D. 1 , YukimasaHatachi , MD 1 , Hiroshi Kubo , MD, PhD 1 , Lusamba K. Nadine , Phar. B 1 , Hidekazu Nishimura , MD, PhD

“Serine Protease Inhibitors Suppress Influenza Virus Infection In Human Airway Epithelial Cells”. In: B101 AIRWAY EPITHELIAL CELL ACTIVATION AND DYSFUNCTION. ed.: American Thoracic Society; 2013: A3550-A3550.

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