Isosensitist Medium
40mM Tris pH 9.5
RNAse
DNAseHazardous Substances Policy - Assessment
CHEMICAL HAZARD AND RISK ASSESSMENT
School of Biosciences
Name of supervisor / Prof N Brown / Assessment Number* / 1991Signature
Assessor / Mrs Rita Godfrey / Date of Assessment / 28.1.03
Signature
NotesA School COSHH form in Word is available on the School Server.
Available from the Health and Safety Unit.
Guidance on making an assessment is given in Making a Chemical Hazard and Risk Assessment.
Guidance is also available from Guidance on Completing the Chemical Hazard and Risk Assessment Form.
Use a continuation sheet to expand any section of this form in hard copy version.
1 / LOCATION OF THE WORK ACTIVITY / T1012 / PERSONS WHO MAY BE AT RISK
List names where possible / Post Docs, Post graduates and Technicians in T101
3 / ACTIVITY ASSESSED / Membrane Protein Preparation
4 / MATERIALS INVOLVED / Attach copies of data sheet(s)
NAME / AMOUNT
max/stored / HAZARD / RISK PHRASES / HAZDAT NO*** / BIOSCIENCESNO***
Isosensitist Medium / 500g / Irritant / R37 Irritating to the respiratory system
40mM Tris pH 9.5 / 1 litre / NSH
RNAse / 50µl / Hazard unknown / Avoid contact and inhalation
DNAse / 50µl / Hazard unknown / Avoid contact and inhalation
8% Chaps / 1 litre / NSH
Solubilization Solution
8M Urea
2M Thiourea
2% Chaps
2% SB3-10
40mM Tris
Ready to use solubilization solution i.e
As above plus per 400ul:
0.5% IPG buffer
2mM TBP
100mM DTT
few grains bromophenol blue / 20ml
400ul / Category 3 Carcinogen
Dangerous for the environment
As above / R40 Possible risk of irreversible effects
.R63 Possible risk of harm to the unborn child.
R51/53 Toxic to aquatic organisms, may cause long-term adverse effects in the aquatic environment.
As above
IPG buffer / 1ml / NSH
DTT / 25g / Irritant / R36/38 Irritating to the eyes and respiratory system / 1340
TBP
Bromophenol Blue / 20ml
10g / Corrosive
Highly Flammable
Harmful
NSH / R34 Causes burns
R17 Spontaneously flammable in air.
R21/22 Harmful in contact with skin and if swallowed.
5 / INTENDED USE**
Give brief details and attach protocol/instructions
Isolation of the proteins from bacterial membrane.
See protocol attached
6 / RISKS to HEALTH and SAFETY from INTENDED USE
From personal exposure or hazardous reactions. Refer to OELs, flash points, etc., as appropriate. Are pregnant women, breast-feeding mothers especially at risk?
Pregnant women need to take extra precautions when handling thiourea as this can harm the unborn child.
There is a fire risk from the TBP
There is a risk of exposure to the isosensitist media, DTT and TBP when weighing or measuring out.
There is a risk to the environment from the disposal of thiourea
The risk from handling RNase and DNase is unknown
7 / CONCLUSIONS ABOUT RISKS
Is level of risk acceptable? Can risk be prevented or reduced by change of substance/procedure? Are control measures necessary?
Control measures are necessary when using thiourea and TBP. The risk to the unborn child can be reduced by the pregnant mother avoiding handling thiourea or getting someone else to do that stage of the method for them.
Good chemical practice should eliminate any risks from the others
As the risk from RNAse and DNAse is unknown it would be wise to handle them with caution.
8 / CONTROL MEASURES
Additional to Good Chemical Practice
Avoid creating a dust when weighing out DTT and isosensitist media.
Keep TBP away from sources of ignition and keep the stock in a nitrogen chamber. Once it is in the solution it is safer.
Wear gloves when handling RNAse and DNAse.
Wear protective clothing and gloves when handling thiourea and TBP
Thiourea should be weighed out in fume hood.
9 / INSTRUCTION/TRAINING
Specify course(s) and/or special arrangements.
Instruction in the use of the nitrogen chamber for the first time.
10 / MONITORING
Performance of control measures,
Check the nitrogen chamber and the fume hood are working correctly before use.
Check integrity of gloves during use.
Personal exposure / Health Surveillance
Occupational Health must be notified before commencing this work due to the presence of a carcinogen – thiourea Tel: 42695
11 / WASTE DISPOSAL PROCEDURE
See School Server for Approved Procedure Document on specific Chemical Waste Disposal.
Thiourea and all solutions containing it must not go down the sink. It should be stored in a non-halogenated waste solvent bottle until full and then taken down to Stores for specialist disposal.
Solutions containing TBP can be washed down the sink with plenty of water as the concentration of TBP and the volumes used are so small. Any waste stock TBP would have to be disposed of as specialist waste via the Stores.
All other compounds can be washed down the sink with plenty of water.
12 / REVIEW
Enter the date or circumstances for review of assessment (maximum review interval 5 years)
January 2008
13 / EMERGENCY ACTION
TO CONTROL HAZARDS / To stabilize situation eg spread absorbant on liquid spill; eliminate sources of ignition, etc.
Thiourea - sweep up avoiding creating a dust and transfer to a suitable labelled container. Take to Stores for specialist disposal.
TBP – use paper towels if spillage is small or absorbant powder from the spill kit to mop up and transfer to a suitable labelled container and take to Stores for specialist disposal.
TO PROTECT PERSONNEL / Evacuation, protection for personnel involved in clean-up, Special First Aid
Evacuate the area. Wear protective clothing, gloves and goggles or a face shield
TO RENDER SITE OF EMERGENCY SAFE / Clean-up/decontamination
The site should be washed with water and detergent and mopped up with paper towels. The paper towels used for thiourea should be placed in a sealed bag and taken to Stores for specialist disposal.
CONTACT PHONE
10.10.00
* Prefix T is used for Teaching Assessment Number.
** Please include amount of chemicals used and how.
***Hazdat No is the UNICOSHH datasheet report number.
Biosciences No is the Biosciences data sheet number.
UNICOSHH IS A CHEMICAL DATABASE ON THE HEALTH AND SAFETY UNIT SERVER.
BIOSCIENCES DATA SHEETS ARE AVAILABLE IN THE SCHOOL SAFETY OFFICE.