MCB 7300: MOLECULAR AND CELLULAR BIOLOGY LABORATORY

(Class Number 2992 – 3.0 Credit Hours)-Spring 2015

Time & Place: Mon. & Wed. 3:05 PM – 6:05 PM in the locations listed on the syllabus.

Course Coordinator: Allan Showalter

Instructors: MCB Faculty as listed on the syllabus

Text: None. Handouts and/or assigned readings will be provided by the instructors.

Requirements and Course Description:

Prerequisites: MCB 7200 or permission. The purpose of this course is to expose students to a variety of laboratory techniques used in the broad field of molecular and cellular biology by allowing the student to carry out these techniques in the laboratory.

Grades will be based on the following:

1. Each instructor, with the exceptions of Thuma and King, will evaluate the student based on a 100 point scale (or 200 points in the case of the Wyatt lab and 300 points in the case of the Kim lab, i.e., now Vis, Basu/Showalter, and Carrol labs) using one or more of the following criteria: 1) a written lab report, 2) a laboratory examination, and 3) instructor observation of student preparation and participation in the lab.

2. Each instructor, with the exceptions listed above, will submit student scores to the course coordinator who will total these scores and record the final grade of the student based on a total of 1100 possible points. (Note that the Wyatt and Kim labs will be worth 200 and 300 points respectively, while the other labs will be worth 100 points each). A standard percentage grading system will be used. Thus, 93.3% and above will earn an A, 90-93.3% an A-, 86.7-90% a B+, 83.3-86.7% a B, 80-83.3% a B-, 76.7-80% a C+, 73.3-76.7% a C, 70-73.3% a C-, 66.7-70% a D+, 63.3-66.7% a D, 60-63.3% a D-, and below 60% an F.

Office Hours:

Allan Showalter- By appointment, Porter 504, phone 593-1135.

Email:

Academic Conduct:

The penalty for course-related academic dishonesty (i.e., plagiarism, cheating, etc.) will be failure of the entire course.

Attendance Policy:

Attendance for laboratory is mandatory. All absences must be justified and explained to the instructor and the course coordinator in advance. Each unjustified absence will result in the reduction of the student's final course grade by one full letter grade.

Course web page: http://www.ohio.edu/plantbio/staff/showalte/MCB%20730/


MCB 7300: MOLECULAR AND CELLULAR BIOLOGY LABORATORY

Syllabus - Spring 2015

Instructors: MCB Faculty as listed below. Please take special note where each lab is to be held.

WEEK DATE Laboratory (Instructor-lab location)

1 Jan. 12 Introduction (Wyatt or Showalter -Porter 304)

Jan. 14 Genomic DNA isolation, finding a gene of interest (Wyatt -Porter 304)

2 Jan. 19 Martin Luther King Day (no class)

Jan. 21 Electrophoresis, ligation & transformation (Wyatt -Porter 304)

3 Jan. 26 Blue-white screening and plasmid prep (Wyatt -Porter 304)

Jan. 28 Seahorse I (Hata-Konnecker Research Lab 126)

4 Feb. 2 Restriction digestion and gel electrophoresis (Wyatt -Porter 304)

Feb. 4 Seahorse II (Hata-Konnecker Research Lab 126)

5 Feb. 9 Introduction to DNA sequencing/PCR (Vis-Porter 304)

Feb. 11 DNA sequence analysis (Vis-Porter 304)

6 Feb. 16 RNA isolation, RNA quality, qPCR setup (Basu/Showalter-Porter 304)

Feb. 18 qPCR and qPCR analysis (Basu/Showalter-Porter 304)

7 Feb. 23 Southern blot analysis I (Showalter-Porter 304)

Feb. 25 Southern blot analysis II (Showalter-Porter 304)

8 March 2 Sprink Break (no class)

March 4 Sprink Break (no class)

9 March 9 Bioinformatics tools I (Hua-Porter 304)

March 11 Bioinformatics tools II (Hua-Porter 304)

10 March 16 Western blot analysis I (McCall-ARC 340)

March 18 Western blot analysis II (McCall-ARC 340)

11 March 23 Fluorescence-Activated Cell Sorting Lab (Goetz & Burdick-ARC 259)

March 25 Fluorescence-Activated Cell Sorting Lab (Goetz & Burdick- ARC 259)

12 March 30 Next gen sequencing (Carrol-Porter 304)

April 1 Introduction to microarrays (Carrol-Porter 304)

13 April 6 Fluorescence microscopy lab (Berryman-Porter 304)

April 8 TBD (Showalter-Porter 304)

14 April 13 Confocal Lab I (Thuma-Wilson 014)

April 15 Confocal Lab II (Thuma-Wilson 014)

15 April 20 Diagnostic Hybrids Field Trip (Kevin King-meet in Porter 304)

April 22 Mammalian transfection lab (Lee-Konneker Research Lab 125)

Teaching assistants: Xiao Liu:

Raj Thapa: