Manish Kumar Suthar, Anita Verma, Pawan Kumar Doharey, Shiv Vardan Singh and Jitendra Kumar Saxena*
Single tryptophan of disordered loop from Plasmodium falciparum Purine Nucleoside Phosphorylase: involvement in catalysis and microenvironment.
Division of Biochemistry, Central Drug Research Institute, Chattar Manzil Palace, M. G. road, Lucknow (U.P.) 226001, India.
*Corresponding author: Tel.: 91- 0522-2625932, Fax.: 91-522-2623405, E. mail:
Journal: Applied Biochemistry and Biotechnology
Supplementary material (S-1): Multiple sequence alignment of various nucleoside phosphorylase sequences from different organisms.
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P.berghei_ ...... MDEEQRHIKLSKKHATPVVLVVG...... DPGRVDKIKVLCD..SY
P.yoelii_ ...... MDEEQRHIKLSKKHATPVVLVVG...... DPGRVDKIKVLCD..SY
P.vivax_ ...... MEGEMQRHIKLTKAQTTPVVLVVG...... DPGRVDKVKVLCD..SY
P.falciparum_ ...... MDNLLRHLKISKEQITPVVLVVG...... DPGRVDKIKVVCD..SY
P.chabaudi_ ...... MSGTQRHIKLSSKNATPVALVVG...... DPGRVDKIKLMCD..SY
E.tenella_ ...... MEGVEVQPHIQLRRGQVQPVVLTMG...... DPKRVDEVAALCD..SA
T.gondii_ ...... MQGMEVQPHIRLRKEDVEPVVIIVG...... DPARTEEVANMCE..KK
T.annulata_ ...... MEYEDCKLLVRMGVPRDRVHKAVIVSE...... NLYHFDLFPKIGD..KY
T.parva_ ...... MEYEDCKLLIRMGVPRDRIHKAAIVSE...... NLYHFYIFTKVGK..KY
B.bovis_ ...... MPRNDDQILNRSNIPKGRDYKVAIICG...... DHKRFEIFKEHST..GY
T.brucei_ ....MAASANGSTKGSETDLPIGKDGTTLHLKCKSDELADRIIFVG...... DPGRVDVISGYFDKDSI
L.infectum_ ....MSGSGCGFLGNVDPDLPLTPDGVTYHVACSSERLADRIILVG...... DPGRVKMVSTYLDTGSI
H.sapiens ...... MENGYTYEDYKNTAEWLLS..HTKHRPQVAIICGSGLGGLTDKLTQAQIFDYGEIPNF
B.malayi_ MSTSMQLYDNGKVINPKSYDDLSEIAKSILKQIQLKEVPKIGIICGSGLGSIADFIEQAEILSYTKIPGF
E.coli_ ...... MATPHINAEMGDFADVVLMPG...... DPLRAKYIAETFL.EDA
40 50 60 70 80
P.berghei_ VDLAYNREYKSVECHYKGQKFLCVSHG...... VGSSGCAICFEELINIG......
P.yoelii_ VDLACNREYKSVECHYKGQKFLCVSHG...... VGSAGCAICFEELINIG......
P.vivax_ VDLAYNREYKSVECTYKGQKFLCVSHG...... VGSAGCAICFEELMNNG......
P.falciparum_ VDLAYNREYKSVECHYKGQKFLCVSHG...... VGSAGCAVCFEELCQNG......
P.chabaudi_ IELAHNREYKSIECTYKGAKFLCVSHG...... VGSAGAAICFEELMNIG......
E.tenella_ EPLTFNREFKSANVVKDGQKFTVISHG...... IGCCSSMICFEELIKLG......
T.gondii_ QELAYNREYRSFRVVYDSQPITVISHG...... IGCPGTSIAIEELAYLG......
T.annulata_ HEFNEFLCYKIAEVEVDGEMILLVYHG...... VSGTAASPILRELLEMG......
T.parva_ CEYREFLCYKIAEVEVDGEMILLVYHG...... VSGNAASPIMKELLEMG......
B.bovis_ RDFGTYLCWKGAELFYDGEYILLASHG...... VGSHPTYVVVRELIELG......
T.brucei_ RASRDHREIRFATGTYKGTPVTVISTG...... MGVDNIEIVLNEIHALKEYDMERGQWRHRK.GDAD
L.infectum_ VYEASHREIKVVTGAYHGVPVTVLSTG...... MGTDNIEIVMNEVHILKEYDTQKRRWRARVGDDTP
H.sapiens PRSTVPGHAGRLVFGFLNGRACVMMQGRFHMYEG.YPLWKVTFPVRVFHLLG......
B.malayi_ PISHVIGHKSNLVFGYINEKYVICMQGRFHPYEYGMDLALCAMPVRILHLLG......
E.coli_ REVNNVRGMLGFTGTYKGRKISVMGHG...... MGIPSCSIYTKELITDFG......
90 100 110 120 130 140
P.berghei_ ...... AKVIIRAGSCGSLQPESIKRGDLCVCNAAVREDRVTHMMIHSDFPAVADYEVYSTLLKC
P.yoelii_ ...... AKVIIRAGSCGSLQPESIKRGDLCVCNAAVREDRVTHMMIHSDFPAVADYEVYSTLLKC
P.vivax_ ...... AKVIIRAGSCGSLQPTQMKRGDICICNAAVREDRVSHLMIYSDFPAVADYEVYATLNQV
P.falciparum_ ...... AKVIIRAGSCGSLQPDLIKRGDICICNAAVREDRVSHLLIHGDFPAVGDFDVYDTLNKC
P.chabaudi_ ...... VKAIIRAGSCGSLQPGYIKRGDLCICNAAVREDRVTHLMIYPDYPAVGDFEVYDVLLKC
E.tenella_ ...... AKCIIRAGTCGSMKPDQVKRGDICVPFAAARDNDVCDLYIPPRMPAVATPRVFQSLLES
T.gondii_ ...... AKVIIRAGTCGSLKPKTLKQGDVCVTYAAVNETGLISNILPEGFPCVATPHVYQALMDA
T.annulata_ ...... VKIVIRCGKCGSYNPCLVNNGDIYICYAAIRDDTVSLAEIDAKYPAVADYDVIDCLVDT
T.parva_ ...... VKIVIRCGKCGTYKPSTVKSGDLSITYASVRDDTSSMAEIDVKYPAVADFDVIDCLVDT
B.bovis_ ...... VKCIIRSGTCGTYRPDIRSTGDICICYGAIRNDSSCLGDADLAYPAVAHPNVFEALRDA
T.brucei_ APSAGPFFDPSTMKIIRLGTCGSPAESVPPLALAVTRHAIGMDNTSLYYSAGTRETSKDQQEIRRIVREQ
L.infectum_ ADPLETPFDPSRVKLIRVGTCGTPNDEVQIGCLAITRYAVGMDNTCQYYDPPAVNHTADVKEVLAKVQAT
H.sapiens ...... VDTLVVTNAAGGLNPK.FEVGDIMLIRDHINLPGFSGQNPLRGPNDERFGDRFPAMSDA
B.malayi_ ...... VETLIVSNAAGGLNSN.FKVGDLMIIKDHIFFPGLAGFSPFVGLHDQRFGERFVSLHEA
E.coli_ ...... VKKIIRVGSCGAVLPHVKLRDVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDA
150 160 170
P.berghei_ A...... EELNVKVHNGISLSSDLYYPHSIIPTRLLDYSK.AN
P.yoelii_ A...... EELNVKVHNGISLSSDLYYPHSIIPTRLLDYSK.AN
P.vivax_ A...... EELKVPVFNGISLSSDMYYPHKIIPTRLEDYSK.AN
P.falciparum_ A...... QELNVPVFNGISVSSDMYYPNKIIPSRLEDYSK.AN
P.chabaudi_ A...... EELEVKAHTGISLSSDLYYPKDILPSRLEDYSK.AN
E.tenella_ G...... KQLGIPLRQGIALTNGLFYGHGKEHIENLTNWS.KF
T.gondii_ A...... KELGIEAASGIGVTQDYFYQNG.ILPSKLEMYS.KC
T.annulata_ A...... EQLGFTTKMGINYSTDLFYRRVCIDDPRDKYTLNNI
T.parva_ A...... EELGIDASLGINYSSDLFYRRTCKDDHRDIYPTYNI
B.bovis_ S...... EALGIPTHLGMSYSSDLFYRRDITNDPRDTYAKYKE
T.brucei_ TGLRAIDIYTSMAHPNITKSICAACDAHNAATGSEADKQQYVIGTTATASGFYGCQGRRVGRFMKHLTVP
L.infectum_ S.LGKVQVYATKAAPAITQGLVDACTALNRKAG.VSEQQPYYVGTTCSGSGFYGCQGRSVGRFRGHLTVP
H.sapiens Y...... DRTMRQRALSTWKQMGEQRELQEGTYVMVAGPSF
B.malayi_ Y...... DPKLRKLAIDVARRQ..KIRIHEGVYAMNGGPQF
E.coli_ A...... KALGIDARVGNLFSADLFYSPDGEMFDVMEKYG...
180 190 200 210 220 230
P.berghei_ VAVVEMELSTLMVMGTLKKVKTGGIFIVDGCPLKWDEGDFDN..VLAADRLE...... NMIKISLEA
P.yoelii_ VAVVEMELSTLMVMGTLKKVKTGGIFIVDGCPLKWDEGDFDN..VLAADRLE...... NMIKISLEA
P.vivax_ VAVVEMEVATLMVMGTLRKVKTGGIFIVDGCPLKWDEGDFDN..NLVPERLE...... NMIKISLET
P.falciparum_ AAVVEMELATLMVIGTLRKVKTGGILIVDGCPFKWDEGDFDN..NLVPHQLE...... NMIKIALGA
P.chabaudi_ VAVVEMELATLMVMGGLSGVKTGGIFIVDGCPLKWKEGDFDE..VLDPKKLE...... EMIRVSMLA
E.tenella_ TDIIDCEFQSLFLVGIGRGIETGGIATVDGSPLQWDQANYDPSGNTVAEGKK...... KMLTVAIHT
T.gondii_ CDVIDMEMSGVLGLCQARGIATCGILAVDGSPLQWDEGDYDATGVKATTGKE...... NMIKITLKA
T.annulata_ ADTEDNETSTVFVLSSLYGAKAGAVLTVDGSPLFWGNGGYAFGEESVEKGIE...... KTMVIGLKA
T.parva_ GDTEESETATIFVLCSLYGAKAGAVMTIDGTPLYWSSGGYIVGEKIVDVGIE...... KMTLLASKA
B.bovis_ VVIEDTDNSAVLLLCNLYGVWGGALCTIDGCPLEWDKGNYQPHTEKMQHGKT...... NMVKLAMHA
T.brucei_ NMVEELGSLKFNLSNGVEVVTNIEMETSAICYLSDMLGYQAGAACVVVSKRVGEKKMFLG.DQLDAAMKR
L.infectum_ HLTEELGDLRFNVSEGEQRVANIEMENSALCYLSNLLGYQAGTVCVVIARRSRTNRTFATPEQLAKGLSR
H.sapiens ETVAECRVLQKLGADAVGMSTVPEVIVARHCGLRVFGFSLITNKVIMDYESLEKANHEEVLAAGKQAAQK
B.malayi_ ESPAELRMLKMLGTDAIGMSTCHEVVVACQMGMRVFGCSLITNIANLDHENAVMVTHEEVLKTGEEAQER
E.coli_ ILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAERQTTFN...... DMIKIALES
_
240
P.berghei_ CAKLSKKY......
P.yoelii_ CARLSEKY......
P.vivax_ CARLAKKY......
P.falciparum_ CAKLATKYA......
P.chabaudi_ CVTLTNRYTPN......
E.tenella_ CTKLTREYSGN......
T.gondii_ CANLRRQY......
T.annulata_ ASVLVKRFCHN......
T.parva_ ASKLSNKYRNNQM......
B.bovis_ AANLSRQLKA......
T.brucei_ CIKIILEALVSA......
L.infectum_ AITIALETLIKE......
H.sapiens LEQFVSILMASIPLPDKAS
B.malayi_ TCSFVSEIVKNL......
E.coli_ VLLGDKE......