LOCUS pRS314-Bir 6696 bp DNA circular 8-AUG-2006
DEFINITION ATCC 77143: Yeast centromere vector pRS314 with TRP1 marker, complete sequence.
KEYWORDS ATCC.
SOURCE
ORGANISM
COMMENT This file is created by Vector NTI
http://www.informaxinc.com/
COMMENT ORIGDB|GenBank
COMMENT VNTDATE|38836800|
COMMENT VNTDBDATE|418207852|
COMMENT VNTNAME|pRS314-BirA-NLS|
COMMENT VNTAUTHORNAME|Demo User|
COMMENT VNTOAUTHORNAME|NCBI Entrez|
COMMENT VNTOAUTHORTEL|(301)496-2475|
COMMENT VNTOAUTHORFAX|(301)480-9241|
COMMENT VNTOAUTHOREML||
COMMENT VNTOAUTHORWWW|http://www3.ncbi.nlm.nih.gov/Entrez/|
COMMENT VNTOAUTHORAD1|National Center for Biotechnology Information|
COMMENT VNTOAUTHORAD2|National Library of Medicine|
COMMENT VNTOAUTHORAD3|Building 38A, Room 8N805|
COMMENT VNTOAUTHORAD4|Bethesda, MD 20894, U.S.A.|
COMMENT VNTREPLTYPE|Phagemid
COMMENT VNTEXTCHREPL|Bacteria
COMMENT VNTEXTCHREPL|Yeast
FEATURES Location/Qualifiers
CDS complement(5122..5982)
/vntifkey="4"
/label=AP(R)
/note="bla beta-lactamase, ampR ampicillin resistance"
CDS 468..1142
/vntifkey="4"
/label=TRP1
/note="TRP1 phosphoribosylanthranilate isomerase"
rep_origin 4364..4364
/vntifkey="33"
/label=PMB1
/note="origin of replication"
misc_feature 6124..6240
/vntifkey="21"
/label=CEN6
/note="centromere VI"
rep_origin 1463..1562
/vntifkey="33"
/label=F1_ORI
/note="origin of replication"
misc_feature 983..997
/vntifkey="21"
/label=ARS1
/note="autonomous replicating sequence"
promoter complement(3921..3940)
/vntifkey="30"
/label=T3_P
/note="T3 promoter"
promoter 1862..1881
/vntifkey="30"
/label=T7_P
/note="T7 promoter"
misc_feature 6241..6615
/vntifkey="21"
/label=ARSH4
/note="autonomous replicating sequence histone 4"
promoter 1924..2303
/vntifkey="29"
/label=PGK
terminator 3509..3882
/vntifkey="43"
/label=CYC1
sig_peptide 2357..2413
/vntifkey="94"
/label=NLS
misc_feature 2414..2521
/vntifkey="21"
/label=HA
CDS 2522..3487
/vntifkey="4"
/label=BirA
BASE COUNT 1801 a 1463 c 1636 g 1796 t
ORIGIN
1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
181 accataaacg acattactat atatataata taggaagcat ttaatagaca gcatcgtaat
241 atatgtgtac tttgcagtta tgacgccaga tggcagtagt ggaagatatt ctttattgaa
301 aaatagcttg tcaccttacg tacaatcttg atccggagct tttctttttt tgccgattaa
361 gaattaattc ggtcgaaaaa agaaaaggag agggccaaga gggagggcat tggtgactat
421 tgagcacgtg agtatacgtg attaagcaca caaaggcagc ttggagtatg tctgttatta
481 atttcacagg tagttctggt ccattggtga aagtttgcgg cttgcagagc acagaggccg
541 cagaatgtgc tctagattcc gatgctgact tgctgggtat tatatgtgtg cccaatagaa
601 agagaacaat tgacccggtt attgcaagga aaatttcaag tcttgtaaaa gcatataaaa
661 atagttcagg cactccgaaa tacttggttg gcgtgtttcg taatcaacct aaggaggatg
721 ttttggctct ggtcaatgat tacggcattg atatcgtcca actgcatgga gatgagtcgt
781 ggcaagaata ccaagagttc ctcggtttgc cagttattaa aagactcgta tttccaaaag
841 actgcaacat actactcagt gcagcttcac agaaacctca ttcgtttatt cccttgtttg
901 attcagaagc aggtgggaca ggtgaacttt tggattggaa ctcgatttct gactgggttg
961 gaaggcaaga gagccccgaa agcttacatt ttatgttagc tggtggactg acgccagaaa
1021 atgttggtga tgcgcttaga ttaaatggcg ttattggtgt tgatgtaagc ggaggtgtgg
1081 agacaaatgg tgtaaaagac tctaacaaaa tagcaaattt cgtcaaaaat gctaagaaat
1141 aggttattac tgagtagtat ttatttaagt attgtttgtg cacttgcctg cggtgtgaaa
1201 taccgcacag atgcgtaagg agaaaatacc gcatcaggaa attgtaaacg ttaatatttt
1261 gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat
1321 cggcaaaatc ccttataaat caaaagaata gaccgagata gggttgagtg ttgttccagt
1381 ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt
1441 ctatcagggc gatggcccac tacgtgaacc atcaccctaa tcaagttttt tggggtcgag
1501 gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg
1561 aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc
1621 gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc
1681 gctacagggc gcgtcgcgcc attcgccatt caggctgcgc aactgttggg aagggcgatc
1741 ggtgcgggcc tcttcgctat tacgccagct ggcgaagggg ggatgtgctg caaggcgatt
1801 aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt
1861 gtaatacgac tcactatagg gcgaattgga gctccaccgc ggtggcggcc gctatcgcta
1921 cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg
1981 gcttgtctgc tcccggcatc cgcttacaga caagctgtgg ccgtctccgg gagctgcatg
2041 tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agagatcccg actcttttct
2101 tctaaccaag ggggtggttt agtttagtag aacctcgtga aacttacatt tacatatata
2161 taaacttgca taaattggtc aatgcaagaa atacatattt ggtcttttct aattcgtagt
2221 ttttcaagtt cttagatgct ttctttttct cttttttaca gatcatcaag gaagtaatta
2281 tctacttttt acaacaaata taaaacaaga tctcgactct agaggatccc ccgggctgca
2341 ggaattcgcc gccaccatgg gtgctcctcc aaaaaagaag agaaaggtag ctggtatcaa
2401 taaagacatc gccacctatg acgtcccaga ttacgcaagt ttgcccgggt atgatgttcc
2461 tgattatgct agcctcccgg gttacgatgt gcccgactat gcctcacttc caggcagatc
2521 tatgaaggat aacaccgtgc cactgaaatt gattgccctg ttagcgaacg gtgaatttca
2581 ctctggcgag cagttgggtg aaacgctggg aatgagccgg gcggctatta ataaacacat
2641 tcagacactg cgtgactggg gcgttgatgt ctttaccgtt ccgggtaaag gatacagcct
2701 gcctgagcct atccagttac ttaatgctaa acagatattg ggtcagctgg atggcggtag
2761 tgtagccgtg ctgccagtga ttgactccac gaatcagtac cttcttgatc gtatcggaga
2821 gcttaaatcg ggcgatgctt gcattgcaga ataccagcag gctggccgtg gtcgccgggg
2881 tcggaaatgg ttttcgcctt ttggcgcaaa cttatatttg tcgatgttct ggcgtctgga
2941 acaaggcccg gcggcggcga ttggtttaag tctggttatc ggtatcgtga tggcggaagt
3001 attacgcaag ctgggtgcag ataaagttcg tgttaaatgg cctaatgacc tctatctgca
3061 ggatcgcaag ctggcaggca ttctggtgga gctgactggc aaaactggcg atgcggcgca
3121 aatagtcatt ggagccggga tcaacatggc aatgcgccgt gttgaagaga gtgtcgttaa
3181 tcaggggtgg atcacgctgc aggaagcggg gatcaatctc gatcgtaata cgttggcggc
3241 catgctaata cgtgaattac gtgctgcgtt ggaactcttc gaacaagaag gattggcacc
3301 ttatctgtcg cgctgggaaa agctggataa ttttattaat cgcccagtga aacttatcat
3361 tggtgataaa gaaatatttg gcatttcacg cggaatagac aaacaggggg ctttattact
3421 tgagcaggat ggaataataa aaccctggat gggcggtgaa atatccctgc gtagtgcaga
3481 aaaataatga ccatggcatc actagtcgag ggggggcccg gtaccaagat ggcctttggt
3541 gggttgaaga aggaaaaaga cagaaacgac ttaattacct acttgaaaaa agcctgtgag
3601 taaacaggcc ccttttcctt tgtcgatatc atgtaattag ttatgtcacg cttacattca
3661 cgccctcccc ccacatccgc tctaaccgaa aaggaaggag ttagacaacc tgaagtctag
3721 gtccctattt atttttttat agttatgtta gtattaagaa cgttatttat atttcaaatt
3781 tttctttttt ttctgtacag acgcgtgtac gcatgtaaca ttatactgaa aaccttgctt
3841 gagaacgttt tgggacgctc gaaggcttta atttgcaagc ttgtcgacct cgaggggggg
3901 cccggtaccc agcttttgtt ccctttagtg agggttaatt ccgagcttgg cgtaatcatg
3961 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acataggagc
4021 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg aggtaactca cattaattgc
4081 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
4141 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
4201 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
4261 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
4321 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctcggccc
4381 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
4441 ataaagatac caggcgttcc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
4501 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcaatg
4561 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
4621 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
4681 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
4741 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
4801 aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
4861 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
4921 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
4981 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
5041 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
5101 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
5161 ctgtctattt cgttcatcca tagttgcctg actgcccgtc gtgtagataa ctacgatacg
5221 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
5281 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
5341 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
5401 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
5461 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
5521 ccccatgttg tgaaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
5581 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
5641 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
5701 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
5761 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
5821 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
5881 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
5941 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
6001 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
6061 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgggtcctt
6121 ttcatcacgt gctataaaaa taattataat ttaaattttt taatataaat atataaatta
6181 aaaatagaaa gtaaaaaaag aaattaaaga aaaaatagtt tttgttttcc gaagatgtaa
6241 aagactctag ggggatcgcc aacaaatact accttttatc ttgctcttcc tgctctcagg
6301 tattaatgcc gaattgtttc atcttgtctg tgtagaagac cacacacgaa aatcctgtga
6361 ttttacattt tacttatcgt taatcgaatg tatatctatt taatctgctt ttcttgtcta
6421 ataaatatat atgtaaagta cgctttttgt tgaaattttt taaacctttg tttatttttt
6481 tttcttcatt ccgtaactct tctaccttct ttatttactt tctaaaatcc aaatacaaaa
6541 cataaaaata aataaacaca gagtaaattc ccaaattatt ccatcattaa aagatacgag
6601 gcgcgtgtaa gttacaggca agcgatccgt cctaagaaac cattattatc atgacattaa
6661 cctataaaaa taggcgtatc acgaggccct ttcgtc
//