LOCUS Exported File 2546 bp ds-DNA circular SYN 05-MAR-2013
DEFINITION Entry vector for transferring a cloned attL-flanked gene to a
destination vector in the Gateway(R) system.
ACCESSION .
VERSION .
KEYWORDS pENTR221
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 2546)
AUTHORS Invitrogen (Life Technologies)
TITLE Direct Submission
JOURNAL Exported Monday, Apr 08, 2013 from SnapGene 1.5.1
FEATURES Location/Qualifiers
source 1..2546
/organism="synthetic DNA construct"
/lab_host="Escherichia coli"
/mol_type="other DNA"
terminator 268..295
/note="rrnB T2 terminator"
/note="transcription terminator T2 from the E. coli rrnB
gene"
/note="color: #ffffff"
terminator 387..473
/gene="Escherichia coli rrnB"
/note="rrnB T1 terminator"
/note="transcription terminator T1 from the E. coli rrnB
gene"
/note="color: #ffffff"
primer_bind 537..553
/note="M13 fwd"
/note="common sequencing primer, one of multiple similar
variants"
/note="color: #a020f0; direction: RIGHT"
protein_bind 569..667
/gene="mutant version of attL"
/bound_moiety="LR Clonase(TM)"
/note="attL1"
/note="recombination site for the Gateway(R) LR reaction"
/note="color: #31849b"
misc_feature 668..670
/note="ORF insertion site"
/note="color: #cc99ff"
protein_bind 671..770
/gene="mutant version of attL"
/bound_moiety="LR Clonase(TM)"
/note="attL2"
/note="recombination site for the Gateway(R) LR reaction"
/note="color: #31849b"
promoter complement(788..806)
/note="T7 promoter"
/note="promoter for bacteriophage T7 RNA polymerase"
/note="color: #ffffff; direction: LEFT"
primer_bind complement(811..827)
/note="M13 rev"
/note="common sequencing primer, one of multiple similar
variants"
/note="color: #a020f0; direction: LEFT"
CDS 940..1749
/codon_start=1
/gene="aph(3')-Ia"
/product="aminoglycoside phosphotransferase"
/note="KanR"
/note="confers resistance to kanamycin in bacteria or G418
(Geneticin(R)) in eukaryotes"
/note="color: #ccffcc"
/translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
rep_origin 1896..2484
/direction=RIGHT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
/note="color: #ffff00"
ORIGIN
1 ctttcctgcgttatcccctgattctgtggataaccgtattaccgcctttgagtgagctga
61 taccgctcgccgcagccgaacgaccgagcgcagcgagtcagtgagcgaggaagcggaaga
121 gcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggca
181 cgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatacgcgtaccgc
241 tagccaggaagagtttgtagaaacgcaaaaaggccatccgtcaggatggccttctgctta
301 gtttgatgcctggcagtttatggcgggcgtcctgcccgccaccctccgggccgttgcttc
361 acaacgttcaaatccgctcccggcggatttgtcctactcaggagagcgttcaccgacaaa
421 caacagataaaacgaaaggcccagtcttccgactgagcctttcgttttatttgatgcctg
481 gcagttccctactctcgcgttaacgctagcatggatgttttcccagtcacgacgttgtaa
541 aacgacggccagtcttaagctcgggccccaaataatgattttattttgactgatagtgac
601 ctgttcgttgcaacaaattgatgagcaatgcttttttataatgccaactttgtacaaaaa
661 agcaggcaccacccagctttcttgtacaaagttggcattataagaaagcattgcttatca
721 atttgttgcaacgaacaggtcactatcagtcaaaataaaatcattatttgccatccagct
781 gatatcccctatagtgagtcgtattacatggtcatagctgtttcctggcagctctggccc
841 gtgtctcaaaatctctgatgttacattgcacaagataaaaatatatcatcatgaacaata
901 aaactgtctgcttacataaacagtaatacaaggggtgttatgagccatattcaacgggaa
961 acgtcgaggccgcgattaaattccaacatggatgctgatttatatgggtataaatgggct
1021 cgcgataatgtcgggcaatcaggtgcgacaatctatcgcttgtatgggaagcccgatgcg
1081 ccagagttgtttctgaaacatggcaaaggtagcgttgccaatgatgttacagatgagatg
1141 gtcagactaaactggctgacggaatttatgcctcttccgaccatcaagcattttatccgt
1201 actcctgatgatgcatggttactcaccactgcgatccccggaaaaacagcattccaggta
1261 ttagaagaatatcctgattcaggtgaaaatattgttgatgcgctggcagtgttcctgcgc
1321 cggttgcattcgattcctgtttgtaattgtccttttaacagcgatcgcgtatttcgtctc
1381 gctcaggcgcaatcacgaatgaataacggtttggttgatgcgagtgattttgatgacgag
1441 cgtaatggctggcctgttgaacaagtctggaaagaaatgcataaacttttgccattctca
1501 ccggattcagtcgtcactcatggtgatttctcacttgataaccttatttttgacgagggg
1561 aaattaataggttgtattgatgttggacgagtcggaatcgcagaccgataccaggatctt
1621 gccatcctatggaactgcctcggtgagttttctccttcattacagaaacggctttttcaa
1681 aaatatggtattgataatcctgatatgaataaattgcagtttcatttgatgctcgatgag
1741 tttttctaatcagaattggttaattggttgtaacactggcagagcattacgctgacttga
1801 cgggacggcgcaagctcatgaccaaaatcccttaacgtgagttacgcgtcgttccactga
1861 gcgtcagaccccgtagaaaagatcaaaggatcttcttgagatcctttttttctgcgcgta
1921 atctgctgcttgcaaacaaaaaaaccaccgctaccagcggtggtttgtttgccggatcaa
1981 gagctaccaactctttttccgaaggtaactggcttcagcagagcgcagataccaaatact
2041 gtccttctagtgtagccgtagttaggccaccacttcaagaactctgtagcaccgcctaca
2101 tacctcgctctgctaatcctgttaccagtggctgctgccagtggcgataagtcgtgtctt
2161 accgggttggactcaagacgatagttaccggataaggcgcagcggtcgggctgaacgggg
2221 ggttcgtgcacacagcccagcttggagcgaacgacctacaccgaactgagatacctacag
2281 cgtgagcattgagaaagcgccacgcttcccgaagggagaaaggcggacaggtatccggta
2341 agcggcagggtcggaacaggagagcgcacgagggagcttccagggggaaacgcctggtat
2401 ctttatagtcctgtcgggtttcgccacctctgacttgagcgtcgatttttgtgatgctcg
2461 tcaggggggcggagcctatggaaaaacgccagcaacgcggcctttttacggttcctggcc
2521 ttttgctggccttttgctcacatgtt
//