Lmon Arab Biol Cairo ISSN 1110-5372

Lmon Arab Biol Cairo ISSN 1110-5372

Http :w~vwarabiolo~istsor~I4~ International Conference

Erna~l ~ 15-19 April, 2007

Vol (27A) Zoology225—244 ,April,2007 Faculty of Education of Suez,

—Rec. 15/3/2007Suez Canal University, Egypt

APPLICABILITY OF BIOSORPTION TECHNOLOGY TO REMEDIATE METAL-CONTAINING TANNING PROCESS

INDUSTRIAL EFFLUENT

El-Fadaly, H.; El-Gammal, M.I.S.; El-Sayed

and Dma Makia***

*Dept. of Microbiology, Fac. of Agric., Mansoura Univ., Mansoura, Egypt.

**Dept. of Environmental Sciences, Fac. of Sci. of Damietta, Mansoura Univ..

Damietta, Egypt.

Dept. Microbiology. Soil, Water & Environment Res. Institute, ARC, Cairo,

Egypt.

Dept. of Environmental Res., Soil, Water & Environment Res. Institute,

ARC, Cairo, Egypt.

Key Words : Biosorption — mineral — bacterial — chromium — microorganisms.

ABSTRACT

Applicability of biosorption technology to remediate metal-containing tanning process (mineral department) industrial effluent was carried out. The microbial treatment was conducted using two bacterial and two yeast strains. These microbes namely Bacillus megaterlurn, 6SB, Serratia niarcescens, 3STA and Saccharomyces cerevisiae, SC64 and Saccharomyces cerevisiae, SC66. Biosorption of chromium by immobilized cells of S.ccrevisiae, SC64 and immobilized cells of 13. Inegateriurn, 6SB, finally by Biosorption of chromium in continuous system using bioreactor:

Batch systems using shake flask: was used for biosorption of som~ trace metal ions using free microbial cells. The observed results showed that B. megaterium, 6SB and S. cerevisiae, SC66 were the most active strains for Cd removal since they gave 100% removal. The best Cr~’~ removal was achieved by S. cerevisiae, SC64 and B.megatcriuni, 6SB being 95.078 and 94.47 %, respectively. At the same time, the results showed that S. inarcescens, 3STA and S. cerevisiae, SC64 exhibited maximum effect upon Mn removal (100%). The four examined microorganisms exhibited maximum effect upon Pb removal (100%). Selenium removal showed a good result since its remOval by 13. megaterium, 6SB reached 91 .978 %. S. cerevisiae, SC64 showed the highest removal of Zn (43.906%). The results indicated that the major portion of the chromium metal was directly removed at the first 24 hr. Colour removal efficiencies were 47.162, 42.258, 46.129 and 45.484 % for

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B.megateriurn, 6SB, S. marcescens, 3STA, S. cerevisiae, SC64 and S. cercvisiae, SC66 after 24 hr, respectively.

Batch systems using bioreactors was applied to examine the biosorption of chromium by immobilized S.cerevisiae, SC64 and B. megaterium, 6SB. The results showed that the chromium metal content of the tested effluent was highly reduced to 75.83 % after 4 h. The percentage of colour removal reached to the maximum value to be 78.06 after 114 hour. The results obtained showed clearly that the TN was completely assimilated during the first 18 hours of incubation. TOC gradually decreased where they were reduced from 352.79 to 20.70 mg/I, after 54 hr. Highly successful removal of chromium metal was reached by using immobilized Bacillus megaterlum, 6SB (98.11%). The maximum colour removal (66.43 %) was reached at the end of fermentation time (135 h). The TN was highly consumed (82.89 %) after 6 hr of fermentation period.

Biosorption of chromium in continuous systems using bioreactor was also examined. The column (52 cm high and 5.2 cm internal diameter) was filled with 0.69 g of living immobilized cells of B. megaterium, 6SB in the form of calcium alginate beads and working volume was 600 ml of tanning effluent (95 %) in continuous fermentation usmg a feed with TA effluent at a dilution rate (D) of 0.0 17 hf’ and the biomass level in the reactor started with of 0.69 gIl. The higher efficiency in chromium and colour removal was reached at the first three hours being 69.98 %, and 39.93 %, respectively. The biomass was reached to its maximum value of 19 mg/l. after 24 hr. The chromium metal uptake value reached 38.69 mg/g biosorbent from TA industrial effluent, after the first three hours.

Key words: Metal bioremediation, tanning process industnal effluent, Bacillus megaterium, SerratEa marcescens and Saccharomyces cerevisiae, Biosorption of chromium, immobilized cells, batch and continuous system.

INTRODUCTION

The problem of water pollution by toxic heavy metal contamination resulting from humans technological activities has for long presented a challenge Biosorption can be a part of the solution. Some types of biosorbents such as seaweeds, molds, yeasts, bacteria or crab shells are examples of biomass tested for metal biosorption with very encouraging

results. The uptake of heavy jnetals by biomass can in some cases reach up to 50% of the biomass dry weight. New biosorbents can be manipulated for better efficiency and multiple re-use to increase their economic attractiveness (Vi~irtt tinti V~iI~}~y, ~I~)). Biosorptton has been intensively studied in recent years as an economical treatment for metal recovery from dilute industrial effluents. Biosorption

• Applicability of biosorption technology to remediate metal-containing tanning process industrial efflueut
  مجلة إتحاد البيولوجين العرب القاهرة, الصفحات 225-224, 1/4/2007
  أ.د.حسين عبدالله محمد الفضالى
  أ.د.مى ابراهيم عبد العزيز الجمال
  دينا عبد الحليم مكية-محطة مياه شرب
  سمير على السيد -مركز البحوث الزراعية