Lab-exam -2 Review S1L

Enzymes

Know the name of the enzyme, subtrates and product ,indicator for the product

Enzyme specificity-to which substrate was more specific lactose or maltose

Enzyme pH, which is the best pH range and the absorbance for the best pH range

What happens to the enzyme at 80-100* temperature?

Importance of cofactor and inhibitor EDTA for enzyme

Cellular Respiration

What gas was measured as a measure of metabolic rate? And why was potassium hydroxide placed in the respiration chamber?

Unit of measurement of metabolic rates

How do you compare the oxygen consumption in animals (Fig 1)?

Which beans produced CO2, was it the germinating ones or the boiled ones?

In fermentation why did yeast prefer 10% sucrose tha10 % glucose?

And what gas was observed as a indicator for anaerobic respiration?

Photosynthesis

Usage of spectroscope is to measure wavelengths. What are the wavelength numbers for blue, green and red light?

In spectrophotometer what wavelength showed 90-100% T for carotene extract and spinach extract?

Name the process by which pigments of plant can be separated? What are the 4 different pigments of spinach leaf?

Which pigment was most soluble and which was least soluble?

Why did the green leaf produce starch?

Will plants produce starch if a portion of the leaf has been covered with foil?

Mitosis and Meosis-cell division

Know to identify the different stages of mitosis and meosis? (plant and animal slide/pictures)?

Biotechnology

What is DNA, Transcription,Translation

DNA electrophoresis- DNA bands, how to read paternity and crime scene gels

Know procedure for DNA extraction and why soap, meat tenderizer and cold ethanol was added to DNA extraction

Unit-14-Prokaryotes

Examples of Prokaryotes and Eukaryotes?

Identify the 3 different shapes of bacteria?

Know the differences between gram positive and gram negative bacteria?

Students can also access the web-site http://iws.ccccd.edu/biopage/Biopage.html and access biology lab to study for the lab –exams.