Janette Mei-Chen Sung
27 Sun Song
Milpitas, CA 95035
408-802-3528 (cell)
408-942-1612 (home)
QUALIFICATIONS
· 6+ years experience in protein/antibody immunoassay development, polyclonal and monoclonal antibody development, antibody purification & characterization and protein bio-conjugation.
· Solid analyzing laboratory skills in providing strategic experiments in immunoassays, analytical methodology, protein/antibody chemistry, epitope amino acid sequence analysis & protein/antibody labeling.
· Effective organization skills; plan, design and conduct laboratory experiments task
· Strong problem solving ability and record keeping skills
PROFESSIONAL EXPERIENCE
Medarex, Inc., Sunnyvale, CA October ’05 – Present
Biologist / Research Associate III
Protein & Medicinal Chemistry Department
Ø Developed analytical methods / assays for cytotoxics-antibody drug conjugate for targeted cancer therapy. The methods are using ELISA, SEC and IHC-based analytical assays to characterize drug conjugate in terms of stability, specificity, activity & purity.
Ø Developed ELISA methods to screen monoclonal antibodies in order to determine the specificity of various versions of ultra potent toxins.
Ø Determined the pharmacokinetic studies of toxin-antibody conjugate for canine / toxicity study.
Ø Established ELISA methods to determine the stability of toxin-antibody conjugate in serum & plasma.
Ø Developed SEC and IHC analytic methods for characterization of conjugates
Ø Wrote SOPs for all the developed immunoassays and transferred to Quality Control department
Ø Participated in different projects by developing various immunoassays
Ø Purified polyclonal & monoclonal antibodies by Protein A, Protein G or affinity columns.
Ø Characterized purified antibodies by SDS-PAGE, western blot, immunoprecipitation, bovine IgG & endotoxin level using LAL method.
Ø Recorded & documented detailed experiments daily in notebook and also computer system in Microsoft Word, Excel or PowerPoint.
Ø Oral presentations for project meetings weekly.
AnaSpec Inc., San Jose, CA April ’01 – Oct. ‘05
Biologist/Senior Research Associate
Antibody Development Department
- Major responsibilities include
1) Antibody-based kit development based on various immunoassays methodology
2) Protein bioconjugation with fluorophores, dyes, and other chemicals.
3) Protein characterization using SDS-PAGE, ELISA, Western Blotting / ECL, Dot
Blotting and immunoprecipitation
4) Antibody purification by immunoaffinity chromatography, precipitation and Protein A or G purification.
5) Maintenance of human tumor cell lines and cancer cell lysate preparation
6) Antibody epitope selection by amino acid sequence analysis
7) Project reports preparation and oral presentation.
- Assisted in managing all internal antibody projects. This includes protein epitope selection, conjugation (to KLH, BSA, and glutaldehyde), immunization, purification, antibody characterization and technical report for final release.
- Involved in new antibody candidate selection in the following area:
Alzheimer’s disease, apoptosis, cancer research, cell signal transduction and tumor
suppressors.
ACADEMIC LABORATORY EXPERIENCE
Oklahoma State University, Environmental Engineering & Water Resources Department,
Stillwater, OK January ’99 – December ‘00
Research Assistant
- Managed equipment operation for HPLC, GC, IC and mass spectrometry.
- Assisted project team in investigating site information and optimizing remedial system.
Project cooperated with US-Army in Pantex, TX to develop methods by biological and
engineering means for TNT/RDX contaminated soil.
EDUCATION
- MS in Environmental Engineering – Oklahoma State University ‘00
- MS in Biology – University of Central Oklahoma ‘98
- BS in Plant Pathology – National Taiwan University ‘94
PUBLICATIONS
1. “A novel duocarmycin-analogue based pro-drug, delivered as an antibody drug conjugate (ADC) for targeted cancer therapy” from Medaex, Inc. published in American Clinical Society ‘06
2. Y.Zhou, J. Sung, A. Hong, K. Chen, J.C. Shih, O.Cases, and T. Vitalis ’03 “A new tool to reveal trace amine signaling pathway: an antibody to β-Phenethylamine.” 43rd American Society for Cell Biology Annual Meeting, San Francisco, CA
3. Y. Zhou, J. Sung, and A. Hong ’03 “The development and application of monoclonal antibodies to phosphoserine and phosphothreonine.” 43rd American Society for Cell Biology Annual Meeting, San Francisco, CA
4. Sung M.C. ’00 “The effectiveness of surfactants for remediation of explosive TNT/RDX contaminated soil.” Master Creative Component, Oklahoma State University, Stillwater, OK
5. Sung M.C. ’99 “Phytoremediation of cadmium, chromium, and mercury-contaminated soil.” Master Literature Thesis, University of Central Oklahoma, Edmond, OK
Reference provided upon request.