Title: Development and Validation of Carotenoids Determination Method in Organic Extracts Obtained from Agro Industrial Waste Materials using Extraction Techniques and High Performance Liquid Chromatography
Name: ImedaRubashvili
IvaneJavakhishvili Tbilisi State University, Tbilisi, Georgia
The manufacture of food products anddietarysupplements using natural food pigmentsand other different natural compounds has been attracted attention in the field of modern food industry. One of the necessary and interesting naturalscompounds are carotenoids which show strong antioxidantandimmunomodulation activities and may prevent degenerative diseases as well.
The present research concerns the development and validation of a new, rapid, modern, effective and selectiveHPLCmethod for determination of carotenoids – beta-carotene and lycopene in organic extracts obtained from tomato, tangerine and orange agro industrial waste materials usingextraction techniques - supercriticalfluid and sequential ultrasonication-assisted extractions.
The method was developed using RP-18 endcappedLiChroCART4 x 250 mm, 5 μm column. The method was validated with respect to robustness, system suitability test, specificity, linearity-range, accuracy, precision, limit of detection (LOD) and quantitation (LOQ). The LOD and the LOQ are 0.081µg/mL and 0.041 µg/mL for beta-carotene, 0.034 µg/mL and 0.085 µg/mL for lycopene, respectively.
The content of each carotenoid per 1 g of dried local agro industrial waste material varies for beta-carotene 0.290 – 10.030µg (tomato peel), 0.445 – 3.972 µg (tangerine peel), 0.833 – 2.455 µg (orange peel) and for lycopene 0.418 – 143.79µg (tomato peel), 0.051 – 179.988 µg (tangerine peel), 0.091 – 0.114 µg (orange peel).
Biography
ImedaRubashvili has completed his PhD at the age of 28 years from Georgian Technical University and postdoctoral study from the University of Liege. He is a scientific researcher at IvaneJavakhishvili Tbilisi State University and the head of validation department of pharmaceutical company “Aversi-rational” Ltd. He has published more than 25 scientific papers and has participated in more than 30 international scientific conferences.He is the member of the council of young scientists of the Georgian National Academy of Sciences.
The project was supported financially ShotaRustaveli National Science Foundation within abroad internship program for young scientists.
Presenting author details
Full name: ImedaRubashvili
Contact number: +995 593 56 02 45
Twitter account:
Linked In account:ImedaRubashvili
Session name/ number: Applied Physical Chemistry
Category: Oral presentation