“ ISOLATION AND CHARACTERIZATION OF BIOACTIVES FROM THE WHOLE PLANT OF ECLIPTA ALBA (Compositae). ”

SYNOPSIS FOR

M.PHARM DISSERTATION

SUBMITTED TO

RAJIV GANDHI UNIVERSITY OF HEALTH SCIENCES

KARNATAKA

BY

BH AMRUTHA SATYA VALLI

I M.PHARM

DEPARTMENT OF PHARMACOGNOSY

PES COLLEGE OF PHARMACY

BENGALURU-560050

(2011-12)

RAJIV GANDHI UNIVERSITY OF HEALTH SCIENCES,

KARNATAKA, BANGALORE

ANNEXURE-II

PROFORMA FOR REGISTRATION OF SUBJECTS FOR DISSERTATION

1. / Name of the candidate and address / BH.AMRUTHA SATYA VALLI
P.E.S. COLLEGE OF PHARMACY
50FT. ROAD,
HANUMANTHNAGAR,
BENGALOORU-560 050
PERMANENT ADDRESS
C/O N.RAVIKANTH
D.NO:139,LAKE SHORE GARDENS,
4TH B-CROSS,THINDLU,VIDYARANYAPURA
BANGALORE-560097
2. / Name of Institution / P.E.S. COLEGE OF PHARMACY
50FT. ROAD,
HANUMANTH NAGAR,
BENGALOORU-560 050.
3. / Course of study and subject / MASTER OF PHARMACY IN PHARMACOGNOSY
4. / Date of Admission / June 2010
5. / TITLE OF THE TOPIC:
“ ISOLATION AND CHARACTERIZATION OF BIOACTIVES FROM THE WHOLE PLANT OF ECLIPTA ALBA (Compositae). ’’
6.
/ Brief resume of the intended work:
6.1 Need For Study:
Eclipta alba (Bringaraj) belongs to Compositae family. Eclipta alba is a plant of potential interest as it has been found to show significant activities like anti-aggressive activity 1,2 , hair growth promoting activity 3 , anti-malarial activity 4 , roots and leaves are used as liver tonic 5. Seeds , leaves, whole plant has been used for various studies.
Isolation and characterization of compounds from the plant Eclipta alba needs to be explored in order to find out bioactive molecules. As much activity on isolation from plant extract of whole plant has not been reported. This study is being taken up in this project.
Many compounds have been reported from Eclipta alba. However, no HPTLC or HPLC based standardization is available and also no markers are available commercially. Therefore, our study is to isolate the markers with the help of major peaks/spots, on HPLC/HPTLC and then to develop a method for standardization for Eclipta alba .
Chemical constituents: Thiophene derivatives, the co-occurrence of mono, di tri thiphene acetylenes together with α-terthienyl in the species is noteworthy. The roots are very rich in thiophene acetylenes.They contain the dithiophene derivatives 5`-seneocioyl oxymethylene-2-dithiopene & 5 tigloy oxymethylene-2-dithiopene in addition to 2-5 thiopene. The petroleum ether extract of aerial parts contains a terthienyl aldehyde,ecliptal besides stigmasterol & β-sitosterol, the aerial parts also contain 2-an-geloyloxy methylene-5-dithiophene,5`-isovaleryloxy methylene-2-dithiophene 6 .
Therapeutic uses:
·  Used in gastropathy, anorexia, helminthiasis, opthalmopathy, ulcers, hypertension, fever, jaundice
·  Used in skin diseases , blackening and strengthening of hair.
·  Used in hepatosplenomeglay, lephantiasis
·  Seeds are good for increasing sexual vigour
·  Used for stopping haemorrhages and fluxes.
·  Used for strengthening the gums.
·  Plant is used as diuretic, carminative, haematinic, anthelmentic 6 .
6.2 Review of the Literature:
·  Banji D. et al., (2010) have shown the impact of the aqueous extract of Eclipta alba on maternal aggression in rats 1 .
·  Lobo OJ et al., (2008) have evaluated the Anti-aggressive activity of Eclipta alba in experimental animals 2 .
·  Datta K. et al., (2009) have studied the potential for hair growth promoting activity of Eclipta alba extract 3 .
·  Bapna S. et al., (2007) have studied Anti-malarial activity of Eclipta alba against plasmodium berghei infection in mice 4 .
·  J. Ananthi et al., (2003) have characterised Anti-hyperglycemic activity of Eclipta alba leaf on alloxan induced diabetic rats 7 .
·  Banji O. et al., (2007) have investigated the effect of Eclipta alba on animal models of learning and memory 8 .
·  Christybapita et al., (2007) have enhanced the non-specific immune responses and disease resistance of Oreochromis mossambicus by oral administration of Eclipta alba leaf aqueous extract 9 .
·  Jayathirtha M.G. et al., (2003) have reported the optimization of Wedelolactone accumulation in shoot cultures of Eclipta alba 10 .
·  Singh B. et al., (2001) have performed the Invivo hepatoprotective activity of active fraction from ethanolic extract of Eclipta alba leaves 11 .
·  Thakur VD et al., (2005) have characterised neuropharmacological profile of Eclipta alba (Linn) Hassk 12 .
·  Zhang M. et al., (1997) have carried out Isolation and identification of Eclipta saponin D from Eclipta alba (L.) Hassk 13 .
6.3 OBJECTIVES OF THE STUDY:
1.  Collection and authentication of the plant Eclipta alba .
2.  Extraction of the drug with different solvents.
3.  Isolation of markers corresponding to the major peaks or spots in
TLC/HPTLC.
4.  Characterization of markers by UV, IR, NMR.
5.  Estimation of markers by HPLC/HPTLC
7 Materials and Methods
7.1 SOURCES OF DATA
Journals searched on RGUHS-Digital library, www.ncbi.nlm.nih.gov/pubmed, Text books of Pharmacognosy and Botany and Library of Sami labs Pvt. Ltd.
Place of work: PES College of Pharmacy and Sami Labs Pvt.Ltd., Bengalooru.
7.2 METHOD OF DATA COLLECTION:
Collection of Plant: Whole plant of Eclipta alba will be collected and authenticated.
Extraction: Extraction will be done by using different solvents, Preliminary identification by TLC.
Isolation of Markers The markers will be isolated from the suitable extracts by various chromatographic techniques.
Purity determination and Characterization: The isolated markers will be characterized
by various methods such as TLC, HPLC, G.C., M.P. and by spectroscopic methods such
as UV, NMR, IR, MASS Spectroscopy.
Quantification and Standardization: The extracts will be quantified with respect to the isolated markers and standardized by using analytical methods such as HPTLC/HPLC.
7.3 DOES THE STUDY REQUIRE ANY INVESTIGATION TO
BE CONDUCTED ON PATIENTS OR ANIMLAS?
-NO-
7.4 HAS ETHICAL CLEARANCE BEEN OBTAINED FROM
YOU’RE INSTITUTION IN CASE OF 7.3?
-Not applicable-
Bibliography:
1.  Banji D, Banji OJ, Annamalai AR, M. S. Impact of the aqueous extract of Eclipta alba on maternal aggression in rats. Pak Journal Pharm Sci. 2010 April 23;2:138-42.
2.  Lobo OJ, Banji D, Annamalai AR, R. M. Evaluation of antiaggressive activity of Eclipta alba in experimental animals. Pak Journal Pharm Sci 2008;21(2):195-99.
3.  Datta K, Singh AT, Mukherjee A, Bhat B, Ramesh B, AC. B. Eclipta alba extract with potential for hair growth promoting activity. Journal of Ethnopharmacology. 2009 July 30;124(3):450-56.
4.  Bapna S, Adsule S, Shirshat Mahendra S, Jadhav S, Patil LS, RA. D. Anti-malarial activity of Eclipta alba against Plasmodium berghei infection in mice.. Journal Commun Dis. 2007;39(2):91-4.
5.  Joshi. SG. Medicinal Plants. 2003;1:81
6.  Prajapati PS. A Handbook of Medicinal Plants: A Complete Source.
7.  J. Ananthi, A. Prakasam, Pugalendi. KV. Antihyperglycemic Activity of Eclipta alba Leaf on Alloxan-induced Diabetic Rats. Yale journal of biology and medicine. 2003(76):97-102.
8.  Banji O, Banji D, Annamalai AR, R. M. Investigation on the effect of Eclipta alba on animal models of learning and memory. Indian Journal Physiol Pharmacolology. 2007;51(3):274-78.
9.  Christybapita D, Divyagnaneswari M, RD. M. Oral administration of Eclipta alba leaf aqueous extract enhances the non-specific immune responses and disease resistance of Oreochromis mossambicus. Fish Shellfish Immunology. 2007 Mar 24(4):840-52.
10.  Jayathirtha MG, SH. M. Optimization of wedelolactone accumulation in shoot cultures of Eclipta alba. Indian Journal Exp Biol. 2003;41(12):1476-78.
11.  Singh B, Saxena AK, Chandan BK, Agarwal SG, KK. A. In vivo hepatoprotective activity of active fraction from ethanolic extract of Eclipta alba leaves. Indian Journal Physiol Pharmacology. 2001;45(4):435-41.
12.  Thakur VD, SA. M. Neuropharmacological profile of Eclipta alba (Linn.) Hassk. Journal of Ethnopharmacolology. 2005 Oct 31;102(1):23-31.
13.  Zhang M, Chen YY, Di XH, M. L. Isolation and identification of ecliptasaponin D from Eclipta alba (L.) Hassk. Yao Xue Xue Bao. 1997;32(8):633-34.
14.  Diogo LC, Fernandes RS, Marcussi S, Menaldo DL, Roberto PG, Matrangulo PV, et al. Inhibition of snake venoms and phospholipases A(2) by extracts from native and genetically modified Eclipta alba: isolation of active coumestans. Basic Clin Pharmacolology & Toxicolology. 2009;104(4):293-99.
15.  Teschke R, R. B. Severe hepatotoxicity by Indian Ayurvedic herbal products: a structured causality assessment. Ann Hepatol. 2009;8(3):258-66.
16.  S.N. Yoganarasimhan. Medicinal plants of india; 2000
17.  M.P. Singh. Medicinal Herbs With their Formulations. 2005:365-67.
18. Kirtikar K.R., B.D. B. Indian Medicinal Plants; 1999:1360-63.
08. / NAME OF THE CANDIDATE
/ BH.AMRUTHA SATYA VALLI
09. / SIGNATURE OF THE CANDIDATE /
(BH.AMRUTHA SATYA VALLI)
10. / REMARKS OF THE GUIDE
11.
12. /
11. 1 NAME AND DESIGNATION OF
THEGUIDE
11. 2 SIGNATURE
11. 3 HEAD OF THE DEPARTMENT
11. 4 SIGNATURE
12.1 REMARKS OF THE PRINCIPAL
12.2 SIGNATURE
/
Dr. K.LAKSHMAN
PROFESSOR & HOD,
DEPT. OF PHARMACOGNOSY
P.E.S. COLLEGE OF PHARMACY
BENGALOORU-560 050.
Dr. K.LAKSHMAN
Prof. Dr. S.MOHAN