Name: Date:
Biology – Period Mrs. Jordy
Internet Cloning Activity
1. Open internet explorer and type in the following web address (or use edline: Click and Clone Link):
2. Click on Mimi the mouse to begin
3. “Welcome to the Mouse Cloning Lab” - Slide 1: This cloning process uses three mice. Who are they and what will you use each one for?
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4. “First Let’s Examine the Steps” - Slide 2: Write the 6 steps involved in cloning your mouse.
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5. Step One Slide: The first step is to remove an egg cell from (the mouse’s name) and place it in a Petri dish.
6. Step Two Slide: Explain the procedure process that is used to remove the nucleus from the egg cell. (Be sure to use the terms blunt pipette and sharp pipette in your explanation)
7. Step Three Slide: An enucleated cell is a cell without a nucleus. Therefore the egg cell is now an enucleated cell. Explain the procedure that is used to transfer the somatic cell nucleus to the egg cell. (Be sure to use the terms blunt pipette and sharp pipette in your explanation)
8. Step Four Slide:
a. Once the somatic cell nucleus and the egg cell are places together and given time to adjust, how do you stimulate cell division?
b. You then observe the cells dividing until they reach the 16 cell ball stage called a .
9. Step 5 Slide: Finally you place this ball of cells into the of (mouse’s name) and wait while the pregnancy continues for days.
10. Step 6 Slide: The clone is born and its color will be the came color as (mouse’s name).
Explain why the mouse is this color.
11. Does this really happen? Slide: This experiment really did occur in Hawaii in 1998. While performing this experiment, what did scientists learn was crucial for successful development of the embryo?
Conclusion Questions:
1. What does the Nucleus contain?
2. What is the purpose of the Somatic (Body) Cell Donor?
4. What is the purpose of the Egg Cell Donor?
5. What is the major difference between a somatic cell and an egg cell in term of chromosome numbers?
6. What is the purpose of the Surrogate Mother?
7. In the following scenario:
Egg Cell Donor: Green
Somatic Cells Donor: Purple
Surrogate Mother: Blue
What color will the Mouse Pup be? Why?