Supplementary Material

Injectable 3-D Fabrication of Medical Electronics at the Target Biological Tissues

(MS # SREP-13-03190)

by Chao Jin, Jie Zhang, Xiaokang Li, Xueyao Yang, Jingjing Li, and Jing Liu*

* Correspondence to

Supplementary movies (file attached/ available online)

  1. Movie 1: Electrical stimulations to the frog’s leg tissue

(1) Experimental subject: the left leg of the frog.

(2) Loading of electrical signal: the positive and negative leads of the input signal are directly connected to the frog’s leg tissue.

(3) Setting parameters for the input signal:

Wave form: Square wave;

Voltage amplitude: 2V;

Duty cycle: 20%.

  1. Movie 2: Electrical stimulations to the frog’s leg tissue

(1) Experimental subject: the right leg of the frog.

(2) Loading of electrical signal: the positive and negative leads of the input signal are directly connected to the frog’s leg tissue.

(3) Setting parameters for the input signal:

Wave form: Square wave;

Voltage amplitude: 2V;

Duty cycle: 20%.

  1. Movie 3: Electrical stimulations to the frog’s leg tissue

(1) Experimental subject: the left leg of the frog.

(2) Loading of electrical signal: the positive and negative leads of the input signal are directly connected to the frog’s leg tissue.

(3) Setting parameters for the input signal:

Wave form: Square wave;

Voltage amplitude: 4V;

Duty cycle: 20%.

  1. Movie 4: Electrical stimulations to the frog’s leg tissue

(1) Experimental subject: the right leg of the frog.

(2) Loading of electrical signal: the positive and negative leads of the input signal are directly connected to the frog’s leg tissue.

(3) Setting parameters for the input signal:

Wave form: Square wave;

Voltage amplitude: 4V;

Duty cycle: 20%.

  1. Movie 5: Electrical stimulations to the frog’s sciatic nerve by the injected gelatin

(1) Experimental subject: the left leg of the frog.

(2) Loading of electrical signal: the positive and negative leads of the input signal are connected to the injected gelatin.

(3) Setting parameters for the input signal:

Wave form: Square wave;

Voltage amplitude: 2V;

Duty cycle: 20%.

  1. Movie 6: Electrical stimulations to the frog’s sciatic nerve by the injected gelatin

(1) Experimental subject: the right leg of the frog.

(2) Loading of electrical signal: the positive and negative leads of the input signal are connected to the injected gelatin.

(3) Setting parameters for the input signal:

Wave form: Square wave;

Voltage amplitude: 2V;

Duty cycle: 20%.

  1. Movie 7: Electrical stimulations to the frog’s sciatic nerve by the injected gelatin

(1) Experimental subject: the left leg of the frog.

(2) Loading of electrical signal: the positive and negative leads of the input signal are connected to the injected gelatin.

(3) Setting parameters for the input signal:

Wave form: Square wave;

Voltage amplitude: 4V;

Duty cycle: 20%.

  1. Movie 8: Electrical stimulations to the frog’s sciatic nerve by the injected gelatin

(1) Experimental subject: the right leg of the frog.

(2) Loading of electrical signal: the positive and negative leads of the input signal are connected to the injected gelatin.

(3) Setting parameters for the input signal:

Wave form: Square wave;

Voltage amplitude: 4V;

Duty cycle: 20%.

  1. Movie 9: Electrical stimulations to the frog’s sciatic nerve by the injectable electrode

(1) Experimental subject: the left leg of the frog.

(2) Loading of electrical signal: the positive and negative leads of the input signal are connected to the injectable electrode.

(3) Setting parameters for the input signal:

Wave form: Square wave;

Voltage amplitude: 2V;

Duty cycle: 20%.

  1. Movie 10: Electrical stimulations to the frog’s sciatic nerve by the injectable electrode

(1) Experimental subject: the right leg of the frog.

(2) Loading of electrical signal: the positive and negative leads of the input signal are connected to the injectable electrode.

(3) Setting parameters for the input signal:

Wave form: Square wave;

Voltage amplitude: 2V;

Duty cycle: 20%.

  1. Movie 11: Electrical stimulations to the frog’s sciatic nerve by the injectable electrode

(1) Experimental subject: the left leg of the frog.

(2) Loading of electrical signal: the positive and negative leads of the input signal are connected to the injectable electrode.

(3) Setting parameters for the input signal:

Wave form: Square wave;

Voltage amplitude: 4V;

Duty cycle: 20%.

  1. Movie 12: Electrical stimulations to the frog’s sciatic nerve by the injectable electrode

(1) Experimental subject: the right leg of the frog.

(2) Loading of electrical signal: the positive and negative leads of the input signal are connected to the injectable electrode.

(3) Setting parameters for the input signal:

Wave form: Square wave;

Voltage amplitude: 4V;

Duty cycle: 20%.