Supplemental data legend

Supplemental figure 1. Recapitulation of theigi1 mutants. A. Recapitulation plants phenotypes. The plants were grown for 20 days. From left to right, Col-0, igi1/IGI1, igi1/igi1, IGI1-RC#1, and IGI1-RC#5.B. Relative expression level for the recapitulation lines. Actin was used for normalization and error bars indicated standard deviation.C. The plant heightin 40-day-old igi1/IGI1 mutant and IGI1-RC#1. D.The number of inflorescence in 40-day-old igi1/IGI1 mutant and IGI1-RC#1. Error bars represent the standard errors of the means.

Supplemental figure 2. Cytokinin response in theigi1 mutants. A-D. Real-time PCR analysis related genes of cytokinin. The graph shows relative expression levels of the cytokinininduced gene (ARR4 and ARR5). The PCR reaction was performed using the cDNA of 10-day-old (A and B)and 25-day-old (C and D) Col-0 and igi1 mutants. The actin transcript levels were used for normalization. Error bars indicate standard deviation.Results are the mean of at least three times. E. Induction of callus formation onhypocotyls segments on different concentrations of kinetin.1, 0 nM Kinetin + 50 nM 2,4-D; 2, 50 nM Kinetin + 50 nM 2,4-D; 3, 200 nM Kinetin + 50 nM 2,4-D; 4, 1000 nM Kinetin + 50 nM 2,4-D.

Supplemental figure 3. Real-time PCR analysis genes of genes related to the pytohormone auxin. Graph shows relative expression levels of auxin biosynthesis component(CYP79B2 andCYP79B3), auxin efflux carrier (PIN1), DFL1, auxin induced gene (IAA5), LAX1, LAX3, PID and ARF5. The PCR reaction was performed using the cDNA of 10-day-old plant. The actin transcript levels were used for normalization. Error bars indicate standard deviation.Results are the mean of at least three times.

Supplemental figure 4. Real-rime PCR analysis of genes to related to branching control. Graph shows relative expression levels of branching control genes. MAX1-2, MAX4,BRC1and BRC2 showed no visible detectable expression levels in igi1 mutants. MAX3 was slightly down-regulated in igi1 mutants. The PCR reaction was performed using the cDNA of 10-day-old plant. The actin transcript levels were used for normalization. Error bars indicate standard deviation.Results are the mean of at least three times.

Supplemental table 1. The primer pairs used for thermal asymmetric interlaced polymerase chain reaction (TAIL-PCR). Table shows arbitrary degenerate(AD) primers on the genomic DNA and long specific primers(LB150, LB100 and LB50) on the T-DNA (Liu et al., 1995).

Supplemental table 2. Selected primers used to evaluate the transcript levels of each gene.

Supplmental figure 1

A

B

C D

Supplemental figure 2

A B C D

E

Supplemental figure 3

Supplemental figure 4

Supplemental table 1

Primer name / Primer sequence (5'→3')
Arbitrary degenerate primers on the genomic DNA / AD2 / 5'-NGTCGASWGANAWGAA
AD5 / 5'-SSTGGSTANATWATWCT
Long specific primers on the T-DNA / LB150 / 5'-CACGTCGAAATAAAGATTTCCG
LB100 / 5'-CCTATAAATACGACGGATGCT
LB50 / 5'-ATAATAACGCTGCGGACATCA
N=A, T, C or G S=C or G W=A or T

Supplemental table 2

Gene name / Primer sequence (5'→3')
CYP79B2 / Forward: TGTGGCTATAACCTTAGTGATGCTACT
Reverse: GCCGTTAGAGAGGATCTTCTGAGCGTAAG
CYP79B3 / Forward: ACGACCAAGTCAAGTCTCGGAATGTCGT
Reverse:GGGATCACGTGAGTGTTTCCTAGACGCA
PIN1 / Forward: TGATGCCACCAACAAGTGTGATGACAAG
Reverse: AACGAGCATGACGGCAGGTCCA
DFL1 / Forward: AGAGGAATCACTTAACAGTGTGTA
Reverse: CAGAACCTTCTACTTCATAGAGAGTCT
IAA5 / Forward: TGAAAGTGAGTGTAGATGGAGCTGCA
Reverse: TAGATATCTGTAAGGCTCACTCACAT
LAX1 / Forward: TGGCGGATGGGCCAGTATGACCAACTTC
Reverse: ACTGAAGACTGGCACGTGACAACACA
LAX3 / Forward: AGAGCCATGGCTAGATTACCCGTGG
Reverse: TGTATATCATGGCTTGTGAGGAGGG
PID / Forward: GCAAGACTCCGTTCGTTGCGCCGACT
Reverse: CATCGGCTTCTTGACGACGGAAGA
ARF5 / Forward: TGAGAACTCTACACTCTCTGGTTGCAGCT
Reverse: AGCTGAAGAATCCTGGGAGGATAGA
MAX1 / Forward: ACATCAATGGTGGGAAGTTCTTGATCCA
Reverse:ACCATAAGAGATGGCCATATATACCA
MAX2 / Forward: TCCTGGAGCAGGTCTGTTACAAGAGTG
Reverse: ATAGTGTAGGTAACTCACTCTTCA
MAX3 / Forward: TGGACTCTACCGGAAACTGCAATAGT
Reverse: TGGGCTCACCAACGAATCTTCTAGC
MAX4 / Forward: TGGAGGTTCCAGCATACGTAACG
Reverse: TGATGCTGCACATATCCATCGCTC
ARR4 / Forward: AGCTCGTCTATGGCCAGAGACGGTGG
Reverse: AGGCATACAGTAATCAGTGATGATC
ARR5 / Forward: CCCGAGATGTTAGATATCTCTAACGAC
Reverse:ATCCAGTCATCCCAGGCATAGAGTA
BRC1 / Forward: TTCCCAGTGATTAACCACCAT
Reverse: TCCGTAAACTGATGCTGCTC
BRC2 / Forward: TCAAAGAGAAGAACAAAGACTATGGA
Reverse: CCGAGGTCTCAAATAATCTCATC