INDIAN JOURNAL OF ANIMAL HEALTH

Vol. 50 (1) June,2011

Contents

1.REGULATION OF ESTRADIOL AND OXYTOCIN INDUCED LUTEOLYSIS IN GAROLE EWES

DIPYAMAN SENGUPTA, SIDDHARTHA BASU, PRAMOD NANDI, SUBHASHISH BATABYAL AND DEBASIS MAZUMDAR

2.HPTLC FINGERPRINTING OF POLYPHENOLS FROM DENDROCALAMUS STRICTUS AND ENYDRA FLUCTUANS

A. MAITY, M. CHAND, A. P. K. MAHAPATRA, P. C. BISOI AND M. R. SENAPATI

3.SERUM BIOCHEMICAL STATUS OF CROSSBRED JERSEY BULLS WITH DECREASED REPRODUCTIVE PERFORMANCE

A. P. K. MAHAPATRA, A. MAITY, A. PRASAD AND A. K. KUNDU

4.A COMPARATIVE STUDY ON EFFECT OF GARLIC AND CORIANDER ON PROXIMATE COMPOSITION AND PHYSICO-CHEMICAL PARAMETERS OF CHICKEN SAUSAGE

A. Bali, A. Khan, D. Patra, S. K. Das and D. Bhattacharya

5.Influence of Different Stocking Densities on the Performance of Commercial Broilers

Sujoy Kumar Sikder, Smritish Chandra Majumder AND Gobinda Halder

6.GROSS AND HISTOMORPHOLOGICAL STUDIES ON THE PINEAL GLAND OF GOAT

A.P. MINJ, R.K.GHOSH, P. DAS AND B.PAL

7.A COMPARATIVE STUDY ON DIFFERENT CATHETERS AND SEALSOLUTIONS IN RAT JUGULAR VEIN CANNULATION

S. MONDAL, A. KALIA, A. BARANWAL, S. K. MHUKHOPADHYAYA, S. S. KARNAMAND R. SHARMA

8.EFFECT OF RESTRICTED FEEDING ON THE PERFORMANCE OF BROILERS

Sujoy Kumar Sikder, Smritish Chandra Majumder AND Souvik Mondal

9.EFECTS OF DIFFERENT CALCIUM SOURCES ON THE PERFORMANCE OF JAPANESE QUAIL

Souvik Mondal, V. Lalzarzova, Gautam Samanta

10.FEEDING OF DIFFERENT CALCIUM SOURCES ON THE CARCASS CHARACTERISTICS, PLASMA AND BONE MAJOR MINERAL STATUS OF JAPANESE QUAIL

SOUVIK MONDAL, V. LALZARZOVA, GAUTAM SAMANTA

Indian Journal of Animal Health, Vol. 50 No. 1 (June, 2011)

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REGULATION OF ESTRADIOL AND OXYTOCIN INDUCED LUTEOLYSIS IN GAROLE EWES

DIPYAMAN SENGUPTA, SIDDHARTHA BASU, PRAMOD NANDI,

SUBHASHISH BATABYAL AND DEBASIS MAZUMDAR

Department of Veterinary Gynaecology and Obstetrics

West Bengal University of Animal and Fishery Sciences

Kolkata-700 037

This experiment was conducted to study the effect of estradiol and oxytocin administration during different phase of diestrus in Garole ewes with or without progesterone pre-exposure. Thirty five regular cycling estrous synchronized ewes of 15-20 kg and 2 to 3 years of age were divided into 7 groups of five ewes each. On day 1 (day 0 = day of estrous) 40 mg progesterone was administered to groups 1, 2 and 3 whereas groups 4, 5 and 6 were kept untreated. Estradiol valerate (500 µg) was administered on days 5 & 6 to groups 1 and 4, on days 7 & 8 to groups 2 and 5, on days 9 & 10 to groups 3 and 6. Group 7 served as control and received no treatments. The ewes were observed for heat twice daily by a vasectomized ram after the estradiol treatment and blood progesterone was measured on the third day of estradiol treatment. Groups 1 and 2, pre-exposed to progesterone responded to estradiol treatment on days 5 & 6 and days 7 & 8 by returning to heat on days 8 ± 0.32 and 9.8 ± 0.38 respectively with progesterone concentration of 0.47 ± 0.07 ng/ ml and 0.58 ± 0.05 ng/ml respectively. Groups 4 and 5 had no progesterone pre-exposure and showed normal estrous cycle that did not differ significantly (P>0.05) from control. However, estradiol treatment on day 9 & 10 brought ewes of group 3 and 6 to heat on day 12.2 ± 0.2 and 11.4 ± 0.25 respectively that did not differ significantly (P>0.05). The results indicate that progesterone pre-exposure is required by the endometrium for release of luteolytic dose of PGF2though estradiol and oxytocin has stimulatory effect on endometrium for the release of PGF2.

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HPTLC FINGERPRINTING OF POLYPHENOLS FROM DENDROCALAMUS STRICTUS AND ENYDRA FLUCTUANS

A. MAITY, M. CHAND, A. P. K. MAHAPATRA, P. C. BISOI AND M. R. SENAPATI

Department of Veterinary Biochemistry

College of Veterinary Science and animal Husbandry

O.U.A.T., Bhubaneswar- 751 003

Rapid and sensitive HPTLC method could be routinely employed to determine the polyphenol content of plant samples. In the present study, polyphenols present in both microwave assisted extract (MAE) and conventional extract (CE) of leaves of Dendrocalamusstrictus and whole plant of Enydrafluctuans were analyzed by HPTLC fingerprinting. In D. strictus, the extract in different solvents and different methods showed different number of bands which varied from two to eleven after derivatization with anisaldehyde; whereas, in E. fluctuans, it varied from one to six. Highest eleven bands were shown by conventional extract using mixture solvent (ethanol: : acetone : water : acetic acid, 40:40:19.9:0.1) in D. strictus; whereas in E. fluctuans, highest six bands were shown by both MAE and CE using mixture solvent.

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SERUM BIOCHEMICAL STATUS OF CROSSBRED JERSEY BULLS WITH DECREASED REPRODUCTIVE PERFORMANCE

A. P. K. MAHAPATRA, A. MAITY, A. PRASAD AND A. K. KUNDU

Department of Physiology

College of Veterinary Science and Animal Husbandry

O.U.A.T., Bhubaneswar - 751 033

The present study was aimed at finding the serum biochemical status in bulls with diminished reproductive performance. Serum glucose, total protein, cholesterol, calcium, phosphorus and magnesium were estimated in two groups of bulls; those producing better quality semen (group I) and poor quality semen (group II) (each group comprising 10 bulls). The study revealed that the bulls producing poor quality semen were hyperglycemic, hypoproteinemic and hypocholesterolemic. So far minerals are concerned; the group II animals were hypocalcemic and hyperphosphatemic.

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A COMPARATIVE STUDY ON EFFECT OF GARLIC AND CORIANDER ON PROXIMATE COMPOSITION AND PHYSICO-CHEMICAL PARAMETERS OF CHICKEN SAUSAGE

A. Bali, A. Khan, D. Patra, S. K. Das and D. Bhattacharya

Department of Livestock Products Technology

West Bengal University of Animal and Fishery Sciences

Kolkata-700 037

Chicken sausages were prepared to evaluate the effects of garlic and coriander on proximate composition and physico-chemical parameters during refrigeration storage (4 ± 1°C) on 0th, 3rd, 7th, 14th and 21st day. Based on Mean scores of sensory evaluation of chicken sausages treated with different concentrations (2%, 3% and 5% w/w) of coriander and garlic, 3% w/w garlic and 3% w/w coriander treatment was selected the best concentration for further study. pH, emulsion stability, cooking loss and proximate composition of control and treated samples were evaluated. Garlic and coriander showed highly significant (P<0.01) impact on the proximate composition of chicken sausages but there was no significant effect of storage period on the moisture, protein, fat and total ash content during refrigeration storage. pH showed an increasing trend (P<0.01) at the early part of storage up to 7 days, thereafter decreased gradually in all the treatment groups and control. The emulsion stability and cooking yield were found to be highest for garlic treated chicken sausage followed by coriander and control.

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Influence of Different Stocking Densities on the Performance of Commercial Broilers

Sujoy Kumar Sikder, Smritish Chandra Majumder AND Gobinda Halder

Department of Animal Production and Management

West Bengal University of Animal and Fishery Sciences

Kolkata- 700 037

An experiment was conducted on the performance of commercial broiler chicken under different cage densities. Ninety six 21 days old broiler chickens (uniform weight) were randomly divided into four experimental groups, comprising of three replicates. Each replicate was consisted of 8 birds. The birds were kept under four different cage densities viz. Control group (C) having cage floor area about 11 birds/m2; T1 having cage floor area about 14.3 birds/m2; T2 having cage floor area about 18 birds/m2 and T3 having cage floor area about 21.5 birds/m2. Common Basal diets and same feeding space/bird and watering space/bird had been provided for each group. Superior performance in terms of final body weight was observed in T1 followed by C, T2 and T3 respectively, whereas the highest overall body weight gain was found in T2 group followed by T3, C and T1 and better feed efficiency was found in T2 group followed by T1, T3 and C respectively. Therefore it can be concluded that T2 group having cage floor area about18 bird/m2 showed better performance in terms of body weight gain and feed efficiency.

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GROSS AND HISTOMORPHOLOGICAL STUDIES ON THE PINEAL GLAND OF GOAT

A.P. MINJ, R.K.GHOSH, P. DAS AND B.PAL

Department of Veterinary Anatomy and Histology

West Bengal University of Animal and Fishery Sciences

Kolkata -700 037

The pineal gland in goat was found to be a small-oval elongated structure situated in the mid-line in a depression in between the superior colliculi. It was attached to the roof of the third ventricle with a stem. The gland was surrounded by a capsule which sent trabeculae into the parenchyma. The lobulations were ill-defined. The gland consisted chiefly of two types of cells viz.pinealocytes and astrocytes. The connective tissue consisted chiefly of reticular fibres , collagen and elastic fibres along with blood vessels. The collagen and elastic fibres were sparse.

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A COMPARATIVE STUDY ON DIFFERENT CATHETERS AND SEALSOLUTIONS IN RAT JUGULAR VEIN CANNULATION

S. MONDAL, A. KALIA, A. BARANWAL, S. K. MHUKHOPADHYAYA,

S. S. KARNAMAND R. SHARMA

Department of Veterinary Pathology

West Bengal University of Animal and Fishery Sciences

Kolkata-700 037

A study was designed to investigate the best seal solution for the catheter, implanted in the rat jugular vein for regular sampling. For this total 110 wistar rats were taken for surgery and catheter implantation. Among the seal solutions it was observed that heparinized glycerol solution was better than any other seal solutions used in this study. We found the silicone tube catheter (0.5X1.1) was better than polyurethane catheter (0.3X0.6) for regular and long time sampling or administration of drug/NCE.

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EFFECT OF RESTRICTED FEEDING ON THE PERFORMANCE OF BROILERS

Sujoy Kumar Sikder, Smritish Chandra Majumder AND Souvik Mondal

Department of Animal Production and Management

West Bengal University of Animal and Fishery Sciences,

Kolkata- 700 037

An experiment was conducted on the performance of commercial broiler chicken under different hourly feed restriction. Ninety six 21 days old broiler chickens (uniform weight) were randomly divided into four experimental groups, comprising of three replicates. Each replicate was consisted of 8 birds. The birds were kept under four different hourly feed restriction treatments, viz. Control group (C) having ad libitum feeding for 24 hrs, T1 having 4hrs feed restriction, T2 having 6hrs feed restriction and T3 having 8hrs feed restriction in a day. Common basal diets and same feeding space/bird and watering space/bird had been provided for each group. Best performance in terms of final body weight was observed in Control group followed by T2, T1 and T3 respectively, whereas the weekly gain in body weight during last week i.e. 6th week was highest in T3 followed by T2, T1 and C respectively and better feed efficiency was found in T2 followed by C, T3 and T1 group. Therefore, it can be concluded that the T2 group i.e. having 6 hrs feed restriction showed better performance in terms of body weight gain and feed efficiency.

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EFECTS OF DIFFERENT CALCIUM SOURCES ON THE PERFORMANCE OF JAPANESE QUAIL

Souvik Mondal, V. Lalzarzova, Gautam Samanta

Department of Animal Nutrition

West Bengal University of Animal and Fishery Sciences

37, Kshudiram Bose Sarani, Kolkata-700037

Considering the importance of calcium in the animal body system and growing interest in commercial quail farming worldwide, in the present experiment, different calcium sources like bone meal, oyster shell, dicalcium phosphate, limestone, calcium carbonate, tricalcium phosphate and calcium hydrogen phosphate were taken into consideration to study the performance of Japanese Quail and it was shown that calcium hydrogen phosphate supplementation resulted highest feed consumption and body weight gain among different calcium sources studied in Japanese quail.

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FEEDING OF DIFFERENT CALCIUM SOURCES ON THE CARCASS CHARACTERISTICS, PLASMA AND BONE MAJOR MINERAL STATUS OF JAPANESE QUAIL

SOUVIK MONDAL, V. LALZARZOVA, GAUTAM SAMANTA

Department of Animal Nutrition

West Bengal University of Animal and Fishery Sciences

37, Kshudiram Bose Sarani, Kolkata-700037

A study was conducted on the effect of feeding of different calcium sources on the carcass characteristics, plasma and bone major mineral status of Japanese quail. One hundred eighty two day-old Japanese quail chicks were used for this study and randomly distributed into seven groups of twenty-six in each group. The average percentage of neck, back, wing, thigh, drumstick and giblet in relation to the dressed weight showed no significant difference among the groups. Only the average percentage of breast showed significant difference (P<0.05) among the groups. Percent of breast weight was highest in T6Group having Diet Containing Tricalcium phosphate followed by T2 Group having Diet Containing Oyster shell, T3 Group having Diet Containing Dicalcium phosphate,T1 Group having Diet Containing Bone meal, T7 Group having Diet Containing Calcium Hydrogen phosphate,T5Group having Diet Containing Calcium carbonate and T4Group having Diet Containing Limestone. The plasma calcium and phosphorus level observed in T7 group followed by T3, T1,T4, T5,T6 and T2 groups. The plasma calcium and phosphorus level of T7 and T3 (control) groups at 3 weeks of age was significantly higher (P<0.05) than other treatment groups. No significant differences observed within T1, T4, T5 and T6Group groups. However, T2 group showed lower plasma calcium than other groups. The bone ash and bone calcium percent at 3rd and 6th week of experimental period clearly indicated that the bio-availability of calcium to Japanese quail for bone formation was highest with T7 followed by T3, T2, T5, T4, T1 and T6Group respectively. The bone ash percent and bone calcium percent at 3 weeks and 6 weeks of age in each group followed the same trend and true for bone phosphorus percent as well. So, it may be conclude that the bone ash percent is good indicator for calcium availability for growing Japanese quail.Further it may be conferred that all the calcium sources used were comparable to dicalcium phosphate in regard to carcass yield in Japanese quail , supplementation of different calcium sources in Japanese quail’s diet did not significantly affect the plasma calcium and phosphorus ratio and bioavailability of calcium for bone formation was observed highest from calcium hydrogen phosphate followed by dicalcium phosphate, oyster shell, CaCO3, limestone, bone meal and tricalcium phosphate groups.