Annex I
ICAR Genetic Analysis Working Group (GAWG)
Questionnaire for "Microsatellite based parentage testing"in cattle
- ADDRESS DETAILS (fill out)
Country: ......
Laboratory name: ......
Contact person: ......
Address: ......
Telephone: ......
E-mail: ......
- EDUCATION, TRAINING AND EXPERIENCE OF SUPERVISOR /OPERATORS
- Level of education of the head of the laboratory (tick the box and describe)
Ph.D. in ......
Masters of Science in ......
Bachelors of Science in......
Other ......
None
- Experience of senior operator (tick)
More than 5 years
More than 2 years
Less than 2 years
- CERTIFICATION, LABORATORY PROCEDURES AND EQUIPMENT
- Certification (tick, describe and send copy of the certification to ICAR secretariat)
International certification
......
National certification
......
No certification (please notice: starting 2010 certification will be a minimum requirement)
- If you have no certification describe briefly:
Procedure for handling of samples from arrival to disposal
......
......
......
......
Procedure for storing and retrieving information
......
......
......
......
Procedure for control of cross-contamination
......
......
......
......
Procedure for error and repeatability checking
......
......
......
......
- Equipment (describe)
Type of sequencer(s)
......
......
......
......
......
......
......
Date(s) of purchase
......
Date(s) of last revision
......
- PARTICIPATION AND PERFORMANCE IN RING TESTS
- Participation and performance in international ring-tests (tick)
≥ 2 international ring tests
< 2 international ring tests
last international ring test (tick and describe)
ISAG
Other (describe number of markers, samples, participating countries)
......
......
......
Year of the last international ring test ......
Performance in the last international ring test (Please notice: 1. If you participated to ISAG ring tests, describe only the results obtained with the 12 compulsory ISAG recommended microsatellites; 2. to specify the meaning of “number of genotypes” we specify that a ring test on 20 individuals analysed with 12 microsatellites produces 240 genotypes)
_____ Number of correct genotypes
_____ Number of missing genotypes
_____ Number of incorrect genotypes
Second to last international ring test (tick and describe)
ISAG
Other (describe number of markers, samples, participating countries)
......
......
......
Year of the second to last international ring test ......
Performance in the last international ring test
_____ Number of correct genotypes
_____ Number of missing genotypes
_____ Number of incorrect genotypes
National ring tests
≥ 2 national ring tests
< 2 national ring tests
Last national ring test description
Country of the ring test......
_____ Number of samples
_____ Number of microsatellite markers
_____ Number of participants
_____ Year of the last national ring test
Performance in the last national ring test
_____ Number of correct genotypes
_____ Number of missing genotypes
_____ Number of incorrect genotypes
Second to last national ring test description
Country of the ring test......
_____ Number of samples
_____ Number of microsatellite markers
_____ Number of participants
_____ Year of the second to last national ring test
Performance in the second to last national ring test
_____ Number of correct genotypes
_____ Number of missing genotypes
_____ Number of incorrect genotypes
No ring test participation
- MARKER SET AND NOMENCLATURE
- Use of ISAG or other marker sets (please tick box and eventually describe)
ISAG microsatellite marker set
Additional microsatellite markers
Other microsatellite marker set (please specify) ......
......
......
Other marker panel (e.g., SNP, please specify) ......
......
......
......
- Microsatellite markers typed on all animals
......
......
......
......
......
......
......
......
......
......
......
......
......
......
......
......
Number of animals typed with these markers
in 2005: ......
in 2006: ......
in 2007: ......
in 2008: ......
- Markers typed when the set of markers previously listed does not resolve the parentage test:
......
......
......
......
......
......
......
......
......
......
......
......
Number of animals typed with these markers
in 2005: ......
in 2006: ......
in 2007: ......
in 2008: ......
- Power of exclusion (1 parent) for each marker used:
Number of animals and breed used for the calculation of PE (1 parent)
......
Method (formula and reference) used to calculate PE (1 parent)
......
Marker PE
……….. ………..
………..………..
………..………..
………..………..
……….………..
………..………..
………..………..
………..………..
………..………..
………..………..
………..………..
………..………..
……….………..
………..………..
………..………..
………..………..
………..………..
………..………..
………..………..
………..………..
………..………..
TOTAL………..
- Power of exclusion (2 parent) for each marker used:
Number of animals and breed used for the calculation of PE (2 parent)
......
Method (formula and reference) used to calculate PE (1 parent)
......
Marker PE
……….. ………..
………..………..
………..………..
………..………..
……….………..
………..………..
………..………..
………..………..
………..………..
………..………..
………..………..
………..………..
……….………..
………..………..
………..………..
………..………..
………..………..
………..………..
………..………..
………..………..
………..………..
TOTAL………..
- Nomenclature (please tick box and eventually describe)
ISAG
Other (please specify) ......
......
......
......
1