83-14 kappa light chain

M D M R A P A Q I F G F L L L L F P G T R C D I Q M T Q S P 8

ATGGACATGAGGGCTCCTGCACAGATTTTTGGCTTCTTGTTGCTCTTGTTTCCAGGTACCAGATGTGACATCCAGATGACCCAGTCTCCA

S S L S A S L G E R V S L T C R A S Q D I G G N L Y W L Q Q 38

TCCTCCTTATCTGCCTCTCTGGGAGAAAGAGTCAGTCTCACTTGTCGGGCAAGTCAGGACATTGGTGGTAACTTATACTGGCTTCAGCAG

G P D G T I K R L I Y A T S S L D P G V P K R F S G S R S G 68

GGACCAGATGGAACTATTAAACGCCTGATCTACGCCACATCCAGTTTAGATCCTGGTGTCCCCAAAAGGTTCAGTGGCAGTAGGTCTGGG

S D Y S L T I S S L K S E D F V D Y Y C L Q Y S S S P W T F 98

TCAGATTATTCTCTCACCATCAGCAGCCTTAAGTCTGAAGATTTTGTAGACTATTACTGTCTACAGTATTCTAGTTCTCCGTGGACGTTC

G G G T K L E I K R A D A A P T V S I F P P S S E Q L T S G 128

GGTGGAGGCACCAAGCTGGAAATCAAACGG

G A S V V C F L N N F Y P K D I N V K W K I D G S E R Q N G 158

V L N S W T D Q D S K D S T Y S M S S T L T L T K D E Y E R 188

H N S Y T C E A T H K T S T S P I V K S F N R G E C 214

83-14 IgG2a heavy chain

M G W R W I F L F L L S G T A G V H C Q V Q L Q Q S G P E L 11

ATGGGATGGAGATGGATCTTTCTTTTCCTCCTGTCAGGAACTGCAGGTGTCCATTGCCAGGTTCAGCTGCAGCAGTCTGGACCTGAGCTG

V K P G A L V K I S C K A S G Y T F T N Y D I H W V K Q R P 41

GTGAAGCCTGGGGCTTTAGTGAAGATATCCTGCAAGGCTTCTGGTTACACCTTCACAAACTACGATATACACTGGGTGAAGCAGAGGCCT

G Q G L E W I G W I Y P G D G S T K Y N E K F K G K A T L T 71

GGACAGGGACTTGAGTGGATTGGATGGATTTATCCTGGAGATGGTAGTACTAAGTACAATGAGAAATTCAAGGGCAAGGCCACACTGACT

A D K S S S T A Y M H L S S L T S E K S A V Y F C A R E W A 101

GCAGACAAATCCTCCAGCACAGCCTACATGCACCTCAGCAGCCTGACTTCTGAGAAATCTGCAGTCTATTTCTGTGCAAGAGAGTGGGCT

Y W G Q G T L V T V S A A K T T A P S V Y P L A P G C G D T 131

TACTGGGGCCAAGGGACTCTGGTCACTGTCTCTGCA

T G S S V T L G C L V K G Y F P E P V T L T W N S G S L S S 161

G V H T F P A V L Q S D L Y T L S S S V T V T S S T W P S Q 191

S I T C N V A H P A S S T K V D K K I E P R G P T I K P C 220

McKern et al. Figure S6. Nucleotide and derived amino acid sequences of the light and heavy chain variable regions of the Fab from the anti-insulin receptor monoclonal antibody 83-14. The translated amino acid sequence for the variable regions of the heavy and light chains of 83-14 was deduced from cDNA clones amplified from mRNA isolated from 83-14 hybridoma cells. For the light chain, the amplified region spanned the signal peptide (underlined), the variable region (residues 1-108) and proximal amino acids from the CH1 domain (109-117). For the heavy chain, the amplified region included the signal peptide (underlined), variable region (1-113) and proximal CH1 residues (113-127). The nucleotide sequence for the variable domains are shown below the derived amino acid sequence. The three CDRs in each chain are shaded green, red and cyan. The additional CH1 amino acid sequences for both chains, together with hinge region residues down to the first cysteine, were obtained from published records.