Table S2. Primers used in this study
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Name Sequence (5’-3’)* Restriction sites; gene specificity; purpose
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TASOE1 GTGCATAACCACTACTATGGCAC hp3021 sequence; used for SOE construction of DtatA mutant
TASOE2 atccacttttcaatctatatcTACTTTACTCCTTAATC cat and hp0321-tatA sequence; SOE construction of DtatA mutant
TASOE3 cccagtttgtcgcactgataaCAAGAAAGTTAAGCATG cat and tatA-hp0319 sequence; SOE construction of DtatA mutant
TASOE4 CTAGCATGCCCTATGTGTAAAGG hp0319 sequence; used for SOE construction of DtatA mutant
TB1 atcggatccCCACTTTAAACCCC BamHI; ruvB sequence; construction of tatB mutant
TB2 tcaggatccGAGCGTGCCTTTATAGG BamHI; tatC sequence; construction of tatB mutant
TC1 aataggatccCTGTCGTGGATGTGGTGAAG BamHI; tatB sequence; construction of DtatC mutant
TC2 ggcgcgaattcATCTTCAAACATTCTC EcoRI; tatB-tatC sequence; construction of DtatC mutant
TC3 ctgcaagcttGATGGTTTCTGTAGGAACG HindIII; tatC sequence; construction of tatC mutant
TC4 cgccaagcttCGCTCTTTGTATTCTCTTTG HindIII; tatC sequence; construction of tatC mutant
TC5 acatagtcgacGCGAGTCGTAGTTGAAAG SalI; tatC-queA sequence; construction of DtatC mutant
TC6 gattactcgagAAAGCGATCAAGCGCTGG XhoI; queA sequence; construction of DtatC mutant
TC7 cgaattcatATGTTTGAAGATTTAAAACCG NdeI; tatC sequence; inducible tatC copy
TC8 ggtaccggatccCAAATTCTTTCAAC BamHI; tatC-queA sequence; inducible tatC copy
TC9 GCGTTTGATGGTTTCTGTAGG tatC sequence; qRT-PCR analysis of tatC in tatB mutants
TC10 TTTAACCGCTACCATGACCC tatC sequence; qRT-PCR analysis of tatC in tatB mutants
QA1 gcgcgaattcGATTTAGAAAGCTATG EcoRI; queA sequence; construction of queA mutant
QA2 acagaattcGCCTATCATCGTGCTTAC EcoRI; queA sequence; construction of queA mutant
FbcF1 AGATTGGATTTATACCGCCG trcF (hp1541) sequence; SOE construction of fbcF mutant
FbcF2 atccacttttcaatctatatcCTCCTTAATGATAATTAC cat and trcF-fbcF intergenic sequence; SOE construction of fbcF mutant
FbcF3 cccagtttgtcgcactgataaTGATGGCTAAAGCGTAAG cat and fbcF (hp1540) sequence; SOE construction of fbcF mutant
FbcF4 ATTCGTGATAACCGCTGCGG fbcH (hp1539) sequence; SOE construction of fbcF mutant
Hyd1 agctttccatgccctacgctaaag hp0630 sequence; SOE construction of DhydABCDE mutant
Hyd2 atccacttttcaatctatatcCATCGTAGAACATGACCACT cat and hydA (hp0631) sequence; SOE construction of DhydABCDE
Hyd3 cccagtttgtcgcactgataagcttaaaagcggatgtgcat cat and hp0635 sequence; SOE construction of DhydABCDE mutant
Hyd4 CTTGTATCGTTTGTGGCTTATAG hp0636 sequence; SOE construction of DhydABCDE mutant
BisC1 GATTTCTATGGATATGAACGGG hp0406 sequence; SOE construction of DbisC mutant
BisC2 atccacttttcaatctatatcGATTCTCCTTCAAGCTTGC cat and bisC (hp0407) sequence; SOE construction of DbisC mutant
BisC3 cccagtttgtcgcactgataaGAGTAAACATACTAGCATG cat and bisC (hp0407) sequence; SOE construction of DbisC mutant
BisC4 GCCGCTCATCAGAGTCTCAGG hp0408 sequence; SOE construction of DbisC mutant
HP405 GTAACGGGAATTCTTA hp0405 sequence; verification of tatC complemented mutant
Cat2 aatgggttatctcggcggtcactc cat; verification of tatC complemented mutant
Bla1 agttctgctatgtggcgcgg amplification of bla (ampr) gene; verification of single cross-over
Bla2 accagccagccggaagggcc amplification of bla (ampr) gene; verification of single cross-over
GyrA1 GCTAGGATCGTGGGTGATGT gyrA sequence; standard for qRT-PCR analysis of tatC
GyrA2 TGGCTTCAGTGTAACGCATC gyrA sequence; standard for qRT-PCR analysis of tatC
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