“Genetic Susceptibility at Low Dose Exposure”
Abstracts
31st Annual Meeting
of the European Environmental
Mutagen Society
September 1 – 5, 2001
Ghent, Belgium
Contents
Index4
Symposium 1: Genetic susceptibility14
Symposium 2: DNA repair 18
Workshop 1: Mitosis versus meiosis23
Symposium 3: Ecogenotoxicology28
Symposium 4: Molecular epidemiology & biomonitoring34
Symposium 5: Low doses & thresholds39
Workshop 2: Electromagnetic fields44
Workshop 3: Microarray systems49
Symposium 6: Regulations update & genotoxicity screening54
Symposium 7: Polymorphism in risk assessment and therapy58
Symposium 8: Genotoxicity of metals63
Symposium 9: Chromosomal sensitivity towards genotoxic agents68
Poster session 174
Poster session 2109
Poster session 3146
Author index182
Organising Associations
European Environmental Mutagen Society
Belgian Environmental Mutagen Society
Local Organising Committee
E. Brits (Mol: VITO)
M. De Boeck (Brussels: VUB)
J. de Gerlache (Brussels: Solvay)
L. De Ridder (Ghent: RUG)
M. Kirsch-Volders (Brussels: VUB)
C. Laurent (Liege: ULg)
D. Lison (Louvain-la-Neuve: UCL)
H. Thierens (Ghent: RUG)
F. Van Goethem (Beerse: Janssen Research Foundation)
J. Van Gompel (Beerse: Janssen Research Foundation)
P. Van Hummelen(Leuven : VIB MicroArray Facility)
P. Vanparys (Beerse: Janssen Research Foundation)
L. Verschaeve (Mol: VITO)
H. Veulemans (Leuven: KUL)
A. Vral (Ghent: RUG)
Scientific Committee
H. Autrup (Denmark: University of Aarhus)
J. de Gerlache (Belgium: Solvay)
P. Farmer (United Kingdom: University of Leicester)
M. Kirsch-Volders (Belgium: VUB)
H. Thierens (Belgium: RUG)
P. Vanparys (Belgium: Janssen Research Foundation)
Conference Chairperson
Prof. H. Thierens
Faculty of Medicine - Ghent University
Proeftuinstraat 86, B-9000 Ghent, Belgium
Phone: +32-9-264-66-43, Fax: +32-9-264-66-96
E-mail:
Index
Symposium 1: Genetic susceptibility
GENETIC SUSCEPTIBILITY: AN INTRODUCTION
Angelo AbbondandoloS1/1 Page 15
CYTOGENETIC BIOMARKERS AND GENETIC POLYMORPHISMS
H. Norppa; J. Tuimala; G. Szekely; S. Gundy; A. Hirvonen S1/2 Page 16
DNA REPAIR POLYMORPHISMS: DISCOVERY, PHENOTYPE AND RISK.
Douglas A. Bell, S1/3Page 17
Symposium 2: DNA repair
NUCLEOTIDE EXCISION REPAIR: FROM HUMAN SYNDROMES TO MOUSE MODELS AND DYNAMICS IN LIVING CELLS
J.H.J. Hoeijmakers, D. Hoogstraten, V. van den Boom, E. Citterio, A. B. Houtsmuller,
W. Vermeulen, J. de Boer, and G.T.J. van der Horst. S2/1 Page 19
TRANSCRIPTION-COUPLED REPAIR OF 8-OXOGUANINE IN VARIOUS HUMAN CELLS.
A. Sarasin; J. Cabral-Neto; M. Pastoriza ; F. Le Page. S2/2 Page 20
THE ROLE OF DNA LIGASE IV IN NON-HOMOLOGOUS END JOINING AND DEFECTS IN PATIENTS.
O’Driscoll M, Cerosaletti K, Girard P, Priestly A, Sperling K, Gennery A, Concannon P
Jeggo P. S2/3 Page 21
ROLE OF THE BREAST CANCER SUSCEPTIBILITY GENE Brca2 AND THE Rad51C GENE IN PROVIDING GENOME STABILITY AND PROTECTION AGAINST DNA DAMAGE
M.Z. Zdzienicka S2/4 Page 22
Workshop 1: Mitosis versus Meiosis
MITOSIS : A UNIFYING MODEL FOR THE METAPHASE/ANAPHASE TRANSITION
M. Kirsch-Volders and I. Decordier W1/1 Page 24
CELL CYCLE INSIGHTS OF MOUSE PRIMARY SPERMATOCYTES
P. de Boer and O. van den Broek W1/2 Page 25
DIFFERENCES IN THE PROCESS OF MITOTIC AND MEIOTIC DIVISIONS AND THEIR CONSEQUENCES FOR CHEMICAL EFFECTS.
Ilse-Dore Adler W1/4 Page 27
Symposium 3: Ecogenotoxicology
TOXICITY AND GENOTOXICITY OF HYDROPHOBIC CHEMICALS SAMPLED WITH SEMIPERMEABLE MEMBRANE DEVICES (SPMDs) IN THE AQUATIC ENVIRONMENT
D. Sabaliunas; J. Lazutka; I. Sabaliuniene S3/1 Page 29
CYTOGENETIC STUDIES ON THE EFFECT OF IONISING RADIATION BY SOLID-STAIN AND FISH TECHNIQUES.
J.F. Barquinero; S. Cigarrán; A. Duran; M.R. Caballín, M. Ribas; L. Barrios S3/2 Page 30
ASSESSMENT OF AIR QUALITY IN SILESIA, POLAND BASED ON CHEMICAL ANALYSES AND SALMONELLA ASSAY – NOW AND 5 YEARS AGO
D. Mielyska; E. Siwińska; A. Bubak; L. Kapka S3/3 Page 31
ASSESSMENT OF TOXICITY AND GENOTOXICITY OF INDUSTRIAL EFFLUENTS IN SOUTHERN ITALY
Dell’Aquila; A. Diodati; M. Isidori; M. Lavorgna; A. Mancini S3/4 Page 32
EMOTIONAL STRESS MODIFY GENOME SENSITIVITY TO ENVIRONMENTAL MUTAGENS. PARALLELS IN RESULTS ON MICE AND HUMAN INVESTIGATIONS
F. Ingel S3/5 Page 33
Symposium 4: Molecular epidemiology and biomonitoring
DNA ADDUCTS IN PAH EXPOSED PERSONS MODULATED BY GENETIC POLYMORPHISMS IN CARCINOGEN METABOLIZING ENZYMES
F.J. Van Schooten S4/1 Page 35
TOBACCO SMOKE-INDUCED GENOTOXICITY IN LARYNGEAL CANCER SUBJECTS
K.Szyfter, P.Jałoszyński, J.Banaszewski, M.Pabiszczak, L.Möller, W.Szyfter S4/2 Page36
MICRONUTRIENTS, GENE-DIET INTERACTION AND GENOMIC STABILITY
Michael Fenech S4/3 Page 37
EFFECT OF METABOLIC GENOTYPES ON THE FORMATION OF BUTADIENE-HEMOGLOBIN ADDUCTS
M. Warholm; P. Begemann; A. Colombi; S. Fustinoni; H.G. Neumann; A. Rannug; L. Soleo; J. Swenberg; L. Vimercati S4/4 Page 38
Symposium 5: Low doses and thresholds
THE RISK ASSESSMENT OF GENOTOXIC CHEMICALS
James M. Parry S5/1 Page 40
LOW DOSES AND THRESHOLDS IN HEPATOCARCINOGENESIS
G. Williams, M.D. S5/2 Page 41
SPONTANEOUS MUTATION RATE • THE APPARENT THRESHOLD
R.C. von Borstel S5/3 Page 42
FISH-DETECTED ASYNCHRONOUS REPLICATION IN HUMAN CELLS EXPOSED TO GENOTOXIC AGENTS
C.Z. Cotrim;A. Brás; I.Vasconcelos ;M. Sá da Costa;J.Rueff S5/4 Page 43
Workshop 2: Electomagnetic fields
ARE EXTREME LOW AND RADIOFREQUENCY FIELDS HAZARDOUS TO HUMANS? AN INTRODUCTION.
L. Verschaeve W2/1 Page 45
ELECTROMAGNETIC FIELDS AND CARCINOGENESIS: INTERACTION OF GENOTOXIC AND NON-GENOTOXIC FACTORS
J. Juutilainen W2/2 Page 46
GENOTOXIC EFFECTS OF BOTH ELF AND RADIOFREQUENCY ALONE AND IN COMBINATION WITH EXPOSURE TO CHEMICALS
MR. Scarfì W2/3 Page 47
STATIC AND EXTREMELY LOW FREQUENCY ELECTROMAGNETIC FIELDS: EVALUATION OF CANCER HAZARDS
R. A. Baan W2/4 Page 48
Workshop 3: Microarray systems
TOXICOGENETICS AND TOXICOGENOMICS: PROMISES AND REALIZATIONS.
J.H.M. van Delft W3/1 Page 50
IDENTIFICATION OF GENE EXPRESSION PROFILES IN DIFFERENT MOUSE TISSUES USING CDNA MICROARRAY.
P. Van Hummelen; J. Mathys; K. Marchal; P. Glenisson; Y. Moreau. W3/2 Page 51
NORMALIZATION AND ERROR ANALYSIS OF DNA MICROARRAY DATA: AFFECT ON INTERPRETATION OF BIOLOGICAL RESULTS
Roger E. Bumgarner W3/3 Page 52
POTENTIAL APPLICATION OF DNA-CHIP TECHNOLOGY (MICROARRAY) IN TOXICOLOGY
Silvio Albertini and Laura Suter-Dick W3/4 Page 53
Symposium 6: Regulations update and genotoxicity screening.
GENOTOXICITY ASSESSMENT IN DRUG DISCOVERY AND EARLY DEVELOPMENT
L. Müller S6/1 Page 55
UK COM GUIDANCE ON A STRATEGY FOR TESTING CHEMICALS FOR MUTAGENICITY
David J Tweats S6/2 Page 56
PROGRESS TOWARDS HARMONISATION IN GENOTOXICITY TESTING THROUGH THE INTERNATIONAL WORKSHOPS (IWGT)
D. Kirkland S6/3 Page 57
Symposium 7: Polymorphisms in risk assessment and therapy.
POLYMORPHISMS OF DRUG METABOLISING ENZYMES AND XENOBIOTIC TOXICITY
Magnus Ingelman-Sundberg S7/1 Page 59
GENETIC POLYMORPHISM IN GLUTATHIONE S-TRANSFERASE AND RESPONSE TO XENOBIOTICA
H.Autrup S7/2 Page 60
INTERINDIVIDUAL VARIABILITY IN RESPONSE TO RADIATION EXPOSURE: ANALYSIS OF DNA REPAIR IN CANCER PATIENTS.
C. Alapetite S7/3 Page 61
IMPACT OF POLYMORPHISMS IN PHASE I OR PHASE II ENZYMES ON LYMPHOCYTE DNA ADDUCTS IN POPULATIONS SUFFERING MEDIUM TO LOW EXPOSURE TO PAH.
P. Georgiadis ; J. Topinka, M. Stoikidou; S. Kaila; M. Gioka, K. Katsouyianni, R. Sram; H. Autrup; A. Kyrtopoulos S7/4 Page 62
Symposium 8: Genotoxicology of metals
METAL- AND REDOX- REGULATION OF P53 PROTEIN FUNCTIONS.
P.Hainaut S8/1 Page 64
DETERMINANTS OF THE GENOTOXICITY OF METALLIC COMPOUNDS.
D. Lison S8/2 Page 65
CARCINOGENIC METAL COMPOUNDS: INTERFERENCE WITH DNA REPAIR PROCESSES AND CELL CYCLE CONTROL
A.Hartwig, M.Asmuss, A. Buerkle, I. Ehleben, D. Kostelac, A. Pelzer, T. Schwerdtle S8/3 Page 66
ELUCIDATION OF THE BIOCHEMICAL PATHWAYS INVOLVED IN THE MUTAGENICITY, ASSOCIATED WITH ISOLATED PARTICULATE DEBRIS FROM THE PERI-PROSTHETIC TISSUE OF FAILED PROSTHESIS.
S.Clerkin, C.P.Case. S8/4 Page 67
Symposium 9: Chromosomal sensitivity towards genotoxic agents.
CHROMOSOMAL ABNORMALITIES IN MITOMYCIN C-SENSITIVE CHINESE HAMSTER CELLS DEFECTIVE IN THE Brca2 AND Rad51C GENES
P.P.W. van Buul; A. van Duijn-Goedhart; M. Kraakman-van der Zwet; B.C. Godthelp; M.Z. Zdzienicka S9/2 Page 70
HOW RELIABLE ARE CHROMOSOMAL ABERRATION ASSAYS AS BIOMARKERS OF INDIVIDUAL SENSITIVITY TOWARDS IONISING RADIATION?
A. Vral, H. Thierens, A. Baeyens and L. De Ridder S9/3 Page 71
DISAPPEARENCE OF CHROMOSOMAL ABERRATIONS FROM THE BLOOD Circulation DEPENDS ON THE LOCATION OF IRRADIATED LYMPH NODES
S. Gundy, G. Székely, Zs. Kelecsényi, O. Ésik S9/4 Page 72
ARA A ENHANCEMENT OF CHROMATID BREAKS IN IRRADIATED CHO CELLS: LACK OF CORRELATION WITH DSB REJOINING
P. E. Bryant and C. Finnegan, S9/5 Page 73
Poster Session 1: Genetic susceptibility, DNA repair, ecogenotoxicology.
DIFFERENCES IN MECHANISMS OF APOPTOSIS IN THE HL60 CELL LINE AND SYRIAN HAMSTER EMBRYO (SHE) CELLS.
Alexandre S., Rast C. and Vasseur P. P1/1 Page 75
DEVELOPMENTAL ABNORMALITIES INDUCED BY X-IRRADIATION IN P53 DEFICIENT OR HETEROZYGOUS MICE.
S. Baatout; P. Jacquet; A. Michaux; J. Buset; W. Schoonjans; J. Yan; A. Benotmane; L. de Saint-Georges; C. Desaintes; M. Mergeay P1/2 Page 76
THE P53 CODON 72 SNP AND LUNG CANCER
E. Biros; I. Biros; A. Kohut; I. Kalina; E. Bogyiova; J. Stubna P1/4 Page 78
ASSESSMENT OF CHEMOTHERAPY-INDUCED DNA DAMAGE IN PERIPHERAL BLOOD LEUKOCYTES OF CANCER PATIENTS USING THE ALKALINE COMET ASSAY
N. Kopjar; V. Garaj-Vrhovac; I. Milas P1/5 Page 79
GENETIC SUSCEPTIBILITY TO BLADDER CANCER IN SLOVAK-CAUCASIANS: ROLE OF NAT2 POLYMORPHISM
V. Habalová; L. Klimčáková; J. Šalagovič; I. Kalina; M. Hrivňák; H. Schneider P1/6 Page 80
POLYMORPHISM OF THE GSTM1 GENE ASSOCIATED WITH SUSCEPTIBILITY TO LUNG CANCER IN RELATION TO the duration of smoking IN SLOVAK POPULATION
J. Šalagovič ; J. Štubňa* ; I. Kalina; L. Klimčáková; V. Habalová P1/7 Page 81
A YEAST GENOTOXICITY AND CYTOTOXICITY ASSAY FOR HIGH-THROUGHPUT SCREENING
N. Billinton ; P. Cahill ; A. W. Knight ; R. M. Walmsley P1/9 Page 83
POLYMORPHISMS IN DNA REPAIR GENES, XPB, XPC AND hHR23B, IN POLISH POPULATION – A PRELIMINARY STUDY.
D. Butkiewicz; M. Rusin; M. Pawlas; M. Chorazy P1/10 Page 84
INFLUENCE OF VHL EXPRESSION ON DNA REPAIR
C. Flohr ; E. Weidt ; J. Decker ; B. Epe P1/11 Page 85
DNA REPAIR CAPACITY AND EXPRESSION PROFILES OF DNA REPAIR GENES IN RESTING AND PHA-STIMULATED HUMAN PERIPHERAL BLOOD LYMPHOCYTES
C. Mayer, O. Zelezny, M.C. von Brevern, A. Bach, H. Bartsch and P. Schmezer P1/12 Page 96
PARP INHIBITOR 3-AMINOBENZAMIDE DOES NOT INCREASE THE YIELDS OF CHROMOSOMAL ABERRANT CELLS INDUCED BY BORON NEUTRON CAPTURE REACTION IN V79 CHINESE HAMSTER CELLS
N.G. Oliveira; M. Castro; A.S. Rodrigues; I.C. Gonçalves; R. Cassapo; A.P. Fernandes; T. Chaveca; J.M. Toscano-Rico and J. Rueff P1/14 Page 88
INFLUENCE OF NITRIC OXIDE ON DNA REPAIR
N. Phoa , B. Epe P1/15 Page 89
DNA DAMAGE AND REPAIR EFFICIENCY IN SCHIZOPHRENIC PATIENTS
D. Psimadas , N. Messini-Nikolaki , A. Fortos , S. Tsilimigaki
and S. Piperakis P1/16 Page 90
SEARCHING FOR REPAIR COMPETENCE OF CELLS OF LARYNX CANCER PATIENTS – GENETIC INSTABILITY AND ALLELIC LOSSES IN GENES CONTROLLING CELL CYCLE AND DNA REPAIR.
Stembalska – Kozowska A. , R. Smigiel , T. Krcicki , M. Blin , F. Mirghomizadeh , K. Bartusiak, M. Sasiadek . P1/17 Page 91
COMPARISON OF DIETHYL SULFATE MUTAGENICITY ON FEMALE AND MALE GERM CELLS OF DROSOPHILA MELANOGASTER UNDER DIFFERENT REPAIR CONDITIONS.
J. Hernando; M. A. Comendador; L. M. Sierra. P1/18 Page 92
EXCISION OF PYRIMIDINE RING-RUPTURED 1,N6-ETHENOADENINE BY THYMINE GLYCOL-DNA GLYCOSYLASE
M. Bajek, J.M. Ciela, B. Tudek P1/19 Page 93
UNSCHEDULED DNA SYNTHESIS: MEASUREMENT OF DNA REPAIR IN A HUMAN HEPATOMA CELL LINE (HEPG2 CELLS)
I. Valentin; Y. Lossouarn; V. Thybaud; J-C. Lhuguenot and M-C. Chagnon P1/20 Page 94
genotoxicity detected with comet assay and micronucleus test in Cyprinus carpio specimens exposed in situ to Trasimeno lake waters treated with disinfectants for potabilization.
Buschini A., Martino A., Gustavino B., Monfrinotti M., Poli P., Rossi C., Santoro M. & Rizzoni M. P1/23 Page 97
EFFECTS OF BRUSSELS SPROUTS EXTRACTS AND THE ACTIVE CONSTITUENTS ON OXIDATIVE DNA DAMAGE AND THE ACTIVITY OF NAD(P)H: QUINONE REDUCTASE IN HEPA 1c1c7 CELLS
C.Y. Zhu; S. Loft P1/24 Page 98
THE USE OF IN VITRO ASSAYS TO TEST SOUTH AFRICAN MEDICINAL PLANT EXTRACTS FOR MUTAGENIC ACTIVITY
E.E. Elgorashi; J.L.S. Taylor; L. Regniers; L. Verschaeve; A. Maes; N. De Kimpe; J. van Staden; A. Fossey P1/25 Page 99
genotoxicity evalutation of triterpenes from Sambucus nigra
T. Cangiano, M. Della Greca, A. Fiorentino, A. Gentili, M. Isidori P1/26 Page 100
INDUCTION OF ANEUPLOIDY IN HUMAN CELL LINES BY HORMONES
Mahmood A. Kayani, James, M. Parry P1/28 Page 102
Induction of micronuclei and chromosome non-disjunction after short-term exposure to CARBENDAZIM IN cultured human lymphocytes
Mahmood. R and Parry. J. M P1/29 Page 103
IN SITU MONITORING WITH TRADESCANTIA- MICRONUCLEUS ASSAY ON THE GENOTOXICITY OF URBAN AIR IN SOUTHERN ITALY
F. Cundari ; M. Isidori ; A. Nardelli ; A. Parrella ; O. Pepe P1/30 Page 104
THE MICRONUCLEUS ASSAY IN HAEMOCYTES OF Dreissena polymorpha FOR THE DETECTION OF GENOTOXICITY IN FRESHWATER ENVIRONMENTS
M. Pavlica; G.I.V. Klobuar; R. Erben; D. Papeš P1/31 Page 105
EVALUATION OF GENOTOXIC ACTIVITY OF OXIDIZING TREATMENTS TO REMOVE SIMAZINE FROM WATER
Sueiro R.A.; Suárez S.; Rubio A., Araujo M., Garrido M. J. P1/32 Page 106
Utilization of the Vitotox GENOTOXICITY test and ACUTE AND CHRONIC microbiotests to assess the environmental risks of solid industrial wastes.
A. Van Cauwenberge, P. Bouviez and E. Noël P1/33 Page 107
COMPARATIVE ASSESSMENT OF WEAK GENOTOXIC PESTICIDE EFFECTS IN PLANTS AND HUMAN CELL CULTURES
M. Wilder; B. Volkmer; E. A. Sanders; R. Greinert; E.W. Breitbart; D. Pollet P1/34 Page 108
Poster Session 2: Molecular epidemiology and biomonitoring, low doses and thresholds, genotoxicity of metals.
Potential Genotoxic Risk for humans by the Environmental Contaminant 3-nitrobenzanthrone
V.M. Arlt; C.A. Bieler; M. Wiessler; D.H. Phillips ; H.H. Schmeiser P2/1 Page 110
Chinese herbs nephropathy and urothelial carcinoma: an out-break in Belgium
V.M. Arlt; J.L. Nortier; J.-L. Vanherweghem; H.H. Schmeiser P2/2 Page 111
THE CHANGES OF SPONTANEOUS FREQUENCY OF CHROMOSOMAL ABERRATIONS IN THE 20 YEARS PERIOD IN THE CZECH REPUBLIC POPULATION GROUPS
H. Bavorova; D. Ocadlikova; P. Rössner; R. J. Sram P2/3 Page 112
MICRONUCLEI IN UNCULTURED T-LYMPHOCYTES OF RAILROAD WORKERS EXPOSED TO TRANSIT CHEMICALS
G. Falck; H. Järventaus; T. Kallas ; J. Catalán; L. Pitkämäk; and H. Norppa P2/4 Page 113
Differences in smoking-related DNA adduct levels in tumorous and non-tumorous tissues from lung cancer patients
E. Gyrffy ; Z. Gyri; I. Soltész; S. Kostič; A. Cseke; J. Minárovits,; B.Schoket P2/5 Page 114
BIOLOGICAL SAMPLE COLLECTION AND PROCESSING IN AN ON SITE LABORATORY IN ESTONIAN SHALE OIL MINE - BIOMODEM STUDY
L.E. Knudsen; A. Jensen; J. Kusova; J. Kubackova; V. Muzyka; R. Anzion; P. Scheepers P2/6 Page 115
URINARY MUCONIC ACID AND PHENYL MERCAPTURIC ACID EXCRETION IN ESTONIAN SHALE OIL MINE WORKERS DEPEND ON GST - GENOTYPES
L.E. Knudsen; A. Jensen; S.Loft; H.Autrup; J.Poole P2/7 Page 116
LEUKOCYTE DNA DAMAGE IN FIBERGLASS-REINFORCED PLASTIC WORKERS MEASURED BY THE COMET ASSAY
B. Laffon; E. Pásaro; J. Méndez P2/8 Page 117
ANALYSING MUTATION SPECTRA
P. D. Lewis and J. M. Parry P2/9 Page 118
MICRONUCLEI ANALYSIS IN PERIPHERAL LYMPHOCYTES OF HOSPITAL WORKERS OCCUPATIONALLY EXPOSED TO IONIZING RADIATIONS.
F. Maffei, S. Angelini, G. Cantelli Forti, V. Lodi, F.S. Violante, S. Mattioli, P. Hrelia P2/10 Page 119
INFLUENCE OF PHASE II ENZYMES ON BIOMARKERS OF EXPOSURE AND EFFECT IN SILESIAN CHILDREN EXPOSED TO POLYCYCLIC AROMATIC HYDROCARBONS
D. Mielyska, K. Szyfter, E. Siwińska, L. Kapka, R. Jaskua –Sztul P2/11 Page 120
BIOMONITORING STUDY OF A GROUP OF WORKERS OCCUPATIONALLY EXPOSED TO PAINTS AND SOLVENTS.
Migliore L., R. Bibbiani, Z. Ricevuto, M. Vicentini, N. Serretti P2/12 Page 121
CYTOGENETIC STUDIES IN SOMATIC AND GERM CELLS OF MALE INDIVIDUALS EXPOSED TO STYRENE IN THE WORKPLACE.
Naccarati A.; A. Zanello; R. Scarpato; L. Lastrucci; L. Migliore P2/13 Page 122
A BIOMARKER APPROACH TO DETECT EARLY DNA DAMAGE AND GENOTOXIC RISK OF COMMON ENVIRONMENTAL POLLUTANTS IN ADOLESCENTS
T Nawrot; E Den Hond;HA Roels; L Verschaeve; G Koppen, JA Staessen P2/14 Page 123
Influence of CYP1A2 and NAT2 phenotypes on urinary mutagenicity in cigarette smokers.
S.Pavanello,P. Simioli, S. Lupi, P. Gregorio, and E. Clonfero P2/15 Page 124
A STUDY ON THE EFFECTS OF SEASONAL SOLAR RADIATION ON EXPOSED POPULATIONS
S. Tsilimigaki , N. Messini-Nikolaki , M. Kanariou , and S. Piperakis P2/16 Page 125
DNA DAMAGE-REPAIR IN A POPULATION WITH CHRONIC PSYCHOGENIC STRESS
E. Dimitroglou , M. Zafiropoulou , N. Messini-Nikolaki , S. Ntountounakis , S. Tsilimigaki and S.Piperakis. P2/17 Page 126
BIOLOGICAL DOSIMETRY IN A GROUP OF NUCLEAR POWER PLANT WORKERS IN BELGIUM BY MEANS OF CHROMOSOME PAINTING.
Roncancio C.L.; Laurent C.; Thierens H. & Lambert V P2/18 Page 127
THE INFLUENCE OF OCCUPATIONAL EXPOSURE TO PAHs ON THE EXPRESSION OF p53 AND p21/WAF1 PROTEINS
P. Rössner Jr.; B. Binková; R. J. Šrám P2/19 Page 128
SPERM CHROMATIN STRUCTURE IN WORKERS EXPOSED TO LOW LEVELS OF INORGANIC LEAD
M. Spano, F. Caruso, G. Leter, E. Cordelli, M. Joffe, P. Apostoli, S. Porru, P. Kiss, M. Vanhoorne, F. Comhaire, A. Giwercman, L. Bisanti, W. Zschiesche, J.P. Bonde P2/20 Page 129
BIOMONITORING OF HUMAN POPULATION EXPOSED TO SIMAZINE IN TAP WATER
Suárez S., Rubio A., Sueiro R.A., Garrido M. J P2/21 Page 130
Sister chromatid exchange and proliferativ rate index in A CROATIAN POPULATION occupationalY exposED to pesticides
D. Željei and V. Garaj-Vrhovac P2/22 Page 131
COMPARISON OF Saccharomyces cerevisiae TEST AND COMET ASSAY ON HUMAN LEUKOCYTES IN THE LOWEST EFFECTIVE DOSE OF CHLORINE DISINFECTANTS
Annamaria Buschini, Paola Poli, Luca Pasini, Chiara Alessandrini, Carlo Rossi P2/23 Page 132
Development of an in situ method for micronucleus assay with Hep G2 and CHO cells.
Fessard V.; Valentin I.; Mourot A.; Chagnon M.C.; Poul J.M.; Lhuguenot J.C. P2/24 Page 133
GRISEOFULVIN: DOSE-RESPONSE STUDIES IN HEPATOCARCINOGENESIS MEDIUM-TERM ASSAY AND IN IN VITRO ANEUPLOIDY INDUCTION IN SPLEEN LYMPHOCYTES IN THE RAT
K. Labay ; M. Ould Elhkim ; M. Poul ; G. Jarry ; S. Marteau ; J.M. Poul ; P. Sanders P2/25 Page 134
LOW DOSES OF GAMMA RAYS: MOLECULAR ALTERATION INDUCED IN HUMAN TUMOR CELLS
M. Osmak; A. Brozovi P2/26 Page 135
AN INVESTIGATION OF THE MUTAGENIC DAMAGE THAT IS CAUSED IN HUMAN CELLS BY DEBRIS FROM WORN ORTHOPAEDIC JOINT REPLACEMENTS
W. Niedzwiedz, C.P. Case P2/28 Page 137
APPLICATION OF THE MICRONUCLEUS ASSAY in patients treated withradiouclide therapies: results and limitations.
M. Monsieurs, A. Vral, B. Brans, L. De Ridder, RA Dierckx and H. Thierens P2/30 Page 139
IN VITRO APOPTOSIS TESTING COMPARED TO THE CLINICAL CHARACTERISTICS
J. Philippé, A. Janssens, F. Offner, H. Thierens P2/31 Page 140
MODULATION OF MUTAGENIC ACTIVITY IN MEAT SAMPLES DEEP-FRIED UNDER DIFFERENT CONDITIONS
C. Perez; A. Lopez de Cerain ; J. Bello P2/32 Page 141
INDUCTION OF MICRONUCLEI BY COMBINED TREATMENTS OF HETEROCYCLIC AMINES (HAs) IN V79 CELLS
C. Perez; A. Lopez de Cerain ; L. Alvarez; J. Bello P2/33 Page 142
DEVELOPMENT OF AN IN VITRO ASSAY TO DETECT MUTATION INDUCTION IN THE LACZ TRANSGENE OF MUTATMMOUSE CULTURED SPLENOCYTES
M. Ballantyne; R. Marshall; A. Wolfreys; G. Ellis P2/34 Page 143
IN VITRO DNA ADDUCT FORMATION BY HETEROCYCLIC AMINES, ACTIVATED VIA DIFFERENT METABOLIC PATHWAYS
H.J.J. Moonen ; T.M.C.M. de Kok ; J.C.S. Kleinjans P 2/35 Page 144
History of the Cytogenetic Analysis in the Czech Republic
Z. Smerhovsky; P. Rossner; R.J. Sram, and K. Landa, G. Loprieno P2/36 Page 145
Poster Session 3: Chromosomal sensitivity towards genotoxic agents, mitosis versus meiosis, electromagnetic fields.
A COMPARISON OF THE CYTOGENETIC RESPONSE TO IRRADIATION OF RESTING PERIPHERAL BLOOD LYMPHOCYTES AND EPSTEIN-BARR VIRUS TRANSFORMED LYMPHOBLASTOID CELLS.
A.Baeyens, A. Vral, H. Thierens and L. De Ridder P3/1 Page 147
GENOTOXIC EFFCTS OF TWO PESTICIDES AND THEIR MIXTURES:
IN-VIVO CHROMOSOMAL ABERRATIONS AND MICRONUCLEUS ASSAY
E.N. El-Khatib; and H.A. Rokaya P3/3 Page 149
THE PROTECTIVE EFFECT OF VITAMIN C AGAINST CYFLUTHRIN INDUCED CLASTOGENICITY IN RAT BONE MARROW CELLS
H. N. EL-KHATI0B P3/4 Page 150
CYTOGENETIC BIOMONITORING OF EGYPTIAN WORKERS EXPOSED TO PESTICIDES: MICRONUCLEI ANALYSIS IN PERIPHERAL BLOOD LYMPHOCYTES
H. N. EL-KHATIB and F. M. Hammam P3/5 Page 151
Phenolic compounds FROM red wine are protective against the DNA damaging effect of ionising radiation ex vivo
W. Greenrod; C. Stockley; M. Abbey; M. Fenech P3/6 Page 152
INDIVIDUAL VARIABILITY IN THE YIELD OF CHROMOSOMAL ABERRATIONS AFTER LOW DOSE GAMMA-RAY IRRADIATION
A.Kiuru ; C. Lindholm ; A. Koivistoinen ; R. Mustonen P3/7 Page 153
INFLUENCE OF AGE ON VINBLASTINE-INDUCED CHROMOSOME MALSEGREGATION IN PERIPHERAL LYMPHOCYTES OF FEMALE DONORS.
P. Leopardi, R. Crebelli, F. Marcon, A. Zijno, G. Dobrowolny P3/8 Page 154
MUTAGENICITY OF AMOSITE FIBRES IN THE LUNG OF lacI TRANSGENIC RATS (A REPORT FROM THE FIBRETOX PROJECT)
P. Loli; J. Topinka; M.Hurbankova; P. Georgiadis; T. Wolff; S. A. Kyrtopoulos P3/9 Page 155
Lymphocytes from iodine-131 treated thyroid cancer patients undergo a transient adaptATion towards mitomycin c genotoxicity
O. Monteiro Gil; N.G. Oliveira; A.S. Rodrigues; A. Laires; T.C. Ferreira; E. Limbert; J. Rueff
P3/10 Page 156
CYTOGENETIC BIOMONITORING OF EXTERNAL WORKERS IN THE NUCLEAR INDUSTRY: STUDY OF EXPOSURE EFFECTS AND SUSCEPTIBILITY
H. Thierens ; A. Vral ; M. Barbé; A. Baeyens; L. De Ridder P3/11 Page 157
INTERFERING WITH HISTONE DEACETYLATION WILL CAUSE ANEUPLOIDY IN MAMMALIAN CELLS
D. Cimini, D. Fioravanti, F. Degrassi P3/12 Page 158
APPLICATION OF THE ALKALINE SINGLE CELL GEL ELECTROPHORESIS (SCGE) ASSAY IN ASSESSMENT OF DNA DAMAGE IN PERIPHERAL BLOOD LEUKOCYTES OF RADAR-FACILITY WORKERS
V. Garaj-Vrhovac, N. Kopjar, D. Želježić P3/13 Page 159
Cytogenetic effects of high frequency electromagnetic fields on human lymphocytes in vitro
I.-L. Hansteen ; E. H Kure; P3/14 Page 160
EFFECTS OF LOW-FREQUENCY ELECTROMAGNETIC FIELDS IN HUMAN LYMPHOCYTES
J.Delimaris , S. Tsilimigaki , N. Messini-Nikolaki , G. Ziros and S.M. Piperakis P3/15 Page 161
ABSENCE OF COOPERATIVE EFFECTS IN L929 CELLS FOLLOWING COMBINED EXPOSURE TO MX AND A 50 Hz SINUSOIDAL MAGNETIC FIELD
O. Zeni, A. Perrotta, P. Pisani, M.R. Scarfì P3/16. Page 162
GENOTOXIC EFFECTS OF 50Hz MAGNETIC FIELDS ON HUMAN BLOOD CELLS
A. Testa, L. Stronati, D.Conti, P. Villani, , A. M. Fresegna, F. Russo, G. Lovisolo, C. Marino and E. Cordelli P3/17 Page 163
No interaction of ELF magnetic fields with a chemical aneugen on micronucleus induction in human lymphocytes.
G.R. Verheyen; G. Pauwels; L. Verschaeve; G. Schoeters P3/18 Page 164