GENBANK ACCESSION NUMBER

CLONE

BOX

GLYCEROL STOCK NUMBER

-SENSEDIGEST

DROP-OUT DIGEST

PLASMID

INSERT SIZE

RESISTANCE

STRAIN

REFERENCE

CVU55761

pEGFP-1

GS4, DS2, TS1

258A, 258B, 258C

N/A

N/A

pEGFP-1 (4.2 kb)

N/A

kanamycin

DH5-α

Clontech, discontinued

INFO

Expression vector with MCS upstream of GFP.Does not contain a promoter.

GLYCEROL STOCK GENERATED BY: Rebecca Bernardos

GENBANK ACCESSION NUMBER
PLASMID NAME / gfap Intron1 (zebrafish)
STOCK # / 265A, B, C
LOCATION (BOXES) / GS4, DS3, TS2
-SENSE DIGEST & TRANSCRIPTION / N/A
DROP-OUT DIGEST / EcoRI
VECTOR NAME & SIZE / pCR-BluntII-TOPO (3519 bp)
INSERT SIZE / 4391 bp
ANTIBIOTIC RESISTANCE / Kanamycin
HOST CELL STRAIN / DH5-T1

OTHER INFO/REFERENCES:

Made by RLB:

Genomic DNA cloned from BAC ZK15N1 (#244).

PCR primers (used High Fidelity Taq kit):

Fwd 5’ gaa tctc gaggtaagg act gag gtgatg 3’

extra XhoI

Rev 5’ ggc cgaattcagatggataaggaaacag 3’

extra EcoRI

See the following for vector details:

Bernardos, R.L. & Raymond, P.A. (2006).GFAP transgenic zebrafish. Gene Expression Patterns 6:1007-1013.

GENBANK ACCESSION NUMBER
PLASMID NAME / gfap Intron1:EGFP1
STOCK # / 266A, B, C
LOCATION (BOXES) / GS4, DS3, TS2
-SENSE DIGEST & TRANSCRIPTION / N/A
DROP-OUT DIGEST / XhoIEcoRI
VECTOR NAME & SIZE / pEGFP-1 (4.2 kb)
INSERT SIZE / 4391 bp
ANTIBIOTIC RESISTANCE / Kanamycin
HOST CELL STRAIN / DH5-

OTHER INFO/REFERENCES:

Made by RLB:

gfap Intron1 dropped out of pCR-BluntII-TOPO vector (#265) by XhoIEcoRI cuts. Ligated into XhoI and EcoRI sites in pEGFP-1 (#258).

See the following for vector details:

Bernardos, R.L. & Raymond, P.A. (2006).GFAP transgenic zebrafish. Gene Expression Patterns 6:1007-1013.

GENBANK ACCESSION NUMBER / From BAC ZK15N1
PLASMID NAME / gfap 5’/Exon1 (zebrafish)
STOCK # / 264A, B, C
LOCATION (BOXES) / GS4, DS3, TS1
-SENSE DIGEST & TRANSCRIPTION / N/A
DROP-OUT DIGEST / EcoRI
VECTOR NAME & SIZE / pCR-BluntII-TOPO (3519 bp)
INSERT SIZE / 3032 bp
ANTIBIOTIC RESISTANCE / Kanamycin
HOST CELL STRAIN / DH5-T1

OTHER INFO/REFERENCES:

Made by RLB:

Genomic DNA cloned from BAC ZK15N1 (#244).

PCR primers (used High Fidelity Taq kit):

Fwd 5’ gcggcgaattccacctttgg gat gtagtc 3’

extra EcoRI

Rev 5’ gta agtcgac gag gaacgctgggactcc a 3’

extra SalI

See the following for vector details:

Bernardos, R.L. & Raymond, P.A. (2006).GFAP transgenic zebrafish. Gene Expression Patterns 6:1007-1013.

GENBANK ACCESSION NUMBER / N/A
PLASMID NAME / gfap Intron1-5’/Ex1: EGFP1 (gfap:GFP)
STOCK # / 267A, B, C
LOCATION (BOXES) / GS4, DS3, TS2
-SENSE DIGEST & TRANSCRIPTION / N/A
DROP-OUT DIGEST / EcoRISalI
VECTOR NAME & SIZE / gfap Intron1:EGFP1 (8520 bp)
INSERT SIZE / 3032 bp (gfap 5’/Ex1)
ANTIBIOTIC RESISTANCE / Kanamycin
HOST CELL STRAIN / DH5-

OTHER INFO/REFERENCES:

Made by RLB:

gfap 5’/Ex1 dropped out from pCR-BluntII-TOPO (#264) by EcoRISalI cut, ligated into the SalIEcoRI sites of gfap Intron1:EGFP1 (#266).

***This vector was used to make the Tg(gfap:GFP)mi2001, mi2002, mi2003 lines.

Linearize the vector with XhoI & clean before injecting (30ng/L).

See the following for vector details:

Bernardos, R.L. & Raymond, P.A. (2006).GFAP transgenic zebrafish. Gene Expression Patterns 6:1007-1013.