Functional Genomics: Discovery of Mammalian Genes That Participate

Additional File 1.Genes associated with resistance to lytic reovirus infection identified by tagged sequence mutagenesis

Mutagenized libraries of RIE-1 cells were infected with reovirus type 1 and selected for resistance to lytic infection. The genes that comprise this list are previously named genes with the exception of several genes that have not been named, such as Chr10orf3, as it was recovered independently from 3 separately prepared libraries screened for resistance to reovirus. In other instances, the vector may disrupt two genes, one of which is located off the negative strand in relation to the vector (-). In the first column, Genes, the yellow background indicates recovery of two or more clones independently obtained with the vector inserted into the same gene. In the column, Definitions, a short description of the gene is provided. The assignment of chromosomal location on the rat (R Chr) and human (H Chr) genomes, along with the Rat Genbank Accession # mRNA and the Genbank Accession # Insert are provided. LocusLink or Entrez Gene numbers (Entrez Gene for Rat Gene) provide links to the National Library of Medicine [1] as well as direct hyperlinks to the National Library of Medicine from the web-based publication. Similarly, the accession numbers for the genomic insertion sites of the vector are provided with the Rat Genbank Accession # mRNA[2]. In the column, Virus interaction includes viruses known to physically interact with or require the gene products of a candidate gene (ADV: adenovirus, CSFV: classical swine fever virus, Friend leukemia virus, FLV, IFAV: influenza A virus, hCMV: human cytomegalovirus, HCV: hepatitis C virus, human herpes virus-8: HHV8, HEV: hepatitis E virus, HSV: herpes simplex virus, HcoV: Human coronavirus, reo: polio: poliovirus, reovirus, SV40: simian virus 40, VSV: vesicular stomatitis virus, VV, vaccinia virus).

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2.Entrez Nucleotide [

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