Functional Determinants in Translation Initiation Factors 1, 1A and 2 Discriminate Against

-hairpin loop of eIF1 mediates 40S ribosome binding to regulate initiator tRNAMet recruitment and accuracy of AUG selection in vivo

Pilar Martin-Marcos, Jagpreet Nanda, Rafael E. Luna, Gerhard Wagner, Jon R. Lorsch, and Alan G. Hinnebusch

SUPPLEMENTAL DATA

Table S1. Plasmids used in this study

Plasmid / Description / Source or Reference
YCplac111 / sc LEU2 cloning vector / (1)
YEplac181 / hc LEU2 cloning vector / (1)
pRS315 / lc LEU2 cloning vector / (2)
YCplac22 / sc TRP1 cloning vector / (1)
p1200 / sc URA3 SUI1 in YCp50 / (3)
YEp24 / hc URA3 cloning vector / (4)
pCFB03 / sc LEU2 His-SUI1 in YCplac111 / (5)
p4389 / hc LEU2 His-SUI1 in YEplac181 / (5)
pJCB101 / sc LEU2 SUI1 in YCplac111 / (6)
pCFB04 / hc LEU2 SUI1 in YEplac181 / (5)
pPMB01 / sc LEU2 sui1-K56E in YCplac111 / (6)
pPMB36 / sc LEU2 sui1-K56D in YCplac111 / This study
pPMB02 / sc LEU2 sui1-K60E in YCplac111 / (6)
pPMB37 / sc LEU2 sui1-K60D in YCplac111 / This study
pPMB88 / sc LEU2 sui1-K60A in YCplac111 / This study
p367 / sc URA3 HIS4(ATG)-lacZ / (7)
p391 / sc URA3 HIS4(TTG)-lacZ / (7)
p180 / sc URA3 GCN4-lacZ in YCp50 / (8)
p1780-IMT / hc URA3 SUI2, SUI3, GCD11, IMT4 in YEp24 / (9)
pPMB47 / sc LEU2 His-sui1-K59A in YCplac111 / This study
pPMB48 / sc LEU2 His-sui1-K59E in YCplac111 / This study
pPMB49 / sc LEU2 His-sui1-K59D in YCplac111 / This study
pPMB50 / sc LEU2 His-sui1-K56E,K60E in YCplac111 / This study
pPMB35A / sc LEU2 sui1-I3N in YCplac111 / This study
pPMB35 / lc LEU2 sui1-I3N in pRS315 / This study
pPMB42 / hc LEU2 sui1-I3N in YEplac181 / This study
pPMB38 / hc LEU2 sui1-K56E in YEplac181 / This study
pPMB39 / hc LEU2 sui1-K60E in YEplac181 / This study
pPMB52 / hc LEU2 His-sui1-K59A in YCplac181 / This study
pPMB53 / hc LEU2 His-sui1-K59E in YCplac181 / This study
pPMB54 / hc LEU2 His-sui1-K59D in YCplac181 / This study
pPMB55 / hc LEU2 His-sui1-K56E,K60E in YCplac181 / This study
pDSO9 / sc LEU2 TIF11 in YCplac111 / (10)
p4552 / sc LEU2 TIF11-NDSDG17-21AAAAA in YCplac111 / (11)
pPMB76 / sc TRP1 TIF11 inYCplac22 / This study
pPMB77 / sc TRP1 tif11-NDSDG17-21AAAAA in YCplac22 / This study
pPMB56 / sc LEU2 sui1-N34A in YCplac111 / This study
pPMB57 / sc LEU2 sui1-N34E in YCplac111 / This study
pPMB58 / sc LEU2 sui1-G35E in YCplac111 / This study
pPMB59 / sc LEU2 sui1-G35R in YCplac111 / This study
pPMB60 / sc LEU2 sui1-R36A in YCplac111 / This study
pPMB61 / sc LEU2 sui1-R36G in YCplac111 / This study
pPMB62 / sc LEU2 sui1-R36E in YCplac111 / This study
pPMB63 / sc LEU2 sui1-K37A in YCplac111 / This study
pPMB64 / sc LEU2 sui1-R33A in YCplac111 / This study
pPMB65 / sc LEU2 His-sui1-K37E in YCplac111 / This study
pPMB68 / sc LEU2 His-sui1-R33E in YCplac111 / This study
pPMB71 / hc LEU2 sui1-R36E in YEplac181 / This study
pPMB72 / hc LEU2 sui1-K37A in YEplac181 / This study
pPMB73 / hc LEU2 sui1-R33A in YEplac181 / This study
pPMB74 / hc LEU2 His-sui1-K37E in YEplac181 / This study
pET-SUI1-His / SUI1-His in pET23a / (12)
pPMB111 / sui1-I3N-His in pET23a / This study
pPMB112 / sui1-K60E-His in pET23a / This study
pPMB114 / sui1-R33A-His in pET23a / This study
pPMB115 / sui1-K37E-His in pET23a / This study
pTYB2-eIF1 / SUI1 in PTYB2 / (13)
pPMB96 / sui1-I3N in pTYB2 / This study
pPMB97 / sui1-K60E in pTYB2 / This study
pPMB99 / sui1-R33A in pTYB2 / This study
pPMB100 / sui1-K37E in pTYB2 / This study

Table S2. Yeast strains used in this study

Strain / Genotype / Source
JCY03 / MATa ura3-52 leu2-3 leu2-112 trp1-63 his4-301(ACG) sui1::hisG p1200 (sc URA3 SUI1) / (5)
PMY30 / MATa ura3-52 leu2-3 leu2-112 trp1-63 his4-301(ACG) sui1::hisG pJCB101 (sc LEU2 SUI1) / (6)
PMY31 / MATa ura3-52 leu2-3 leu2-112 trp1-63 his4-301(ACG) sui1::hisG pPMB01 (sc LEU2 sui1-K56E) / (6)
PMY272 / MATa ura3-52 leu2-3 leu2-112 trp1-63 his4-301(ACG) sui1::hisG pPMB36 (sc LEU2 sui1-K56D) / This study
PMY32 / MATa ura3-52 leu2-3 leu2-112 trp1-63 his4-301(ACG) sui1::hisG pPMB02 (sc LEU2 sui1-K60E) / (6)
PMY273 / MATa ura3-52 leu2-3 leu2-112 trp1-63 his4-301(ACG) sui1::hisG pPMB37 (sc LEU2 sui1-K60D) / This study
pMY280 / MATa ura3-52 leu2-3 leu2-112 trp1-63 his4-301(ACG) sui1::hisG pPMB88 (sc LEU2 sui1-K60A) / This study
PMY194 / MATa ura3-52 leu2-3 leu2-112 trp1-63 his4-301(ACG) sui1::hisG p1200 (sc URA3 SUI1) pCFB03 (sc LEU2 His-SUI1) / This study
PMY175 / MATa ura3-52 leu2-3 leu2-112 trp1-63 his4-301(ACG) sui1::hisG p1200 (sc URA3 SUI1) pPMB47 (sc LEU2 His-sui1-K59A) / This study
PMY176 / MATa ura3-52 leu2-3 leu2-112 trp1-63 his4-301(ACG) sui1::hisG p1200 (sc URA3 SUI1) pPMB48 (sc LEU2 His-sui1-K59E) / This study
pMY177 / MATa ura3-52 leu2-3 leu2-112 trp1-63 his4-301(ACG) sui1::hisG p1200 (sc URA3 SUI1) pPMB49 (sc LEU2 His-sui1-K59D) / This study
PMY178 / MATa ura3-52 leu2-3 leu2-112 trp1-63 his4-301(ACG) sui1::hisG p1200 (sc URA3 SUI1) pPMB50 (sc LEU2 His-sui1-K56E-K60E) / This study
PMY274 / MATa ura3-52 leu2-3 leu2-112 trp1-63 his4-301(ACG) sui1::hisG pPMB35 (lc LEU2 sui1-I3N) / This study
PMY40 / MATa ura3-52 leu2-3 leu2-112 trp1-63 his4-301(ACG) sui1::hisG pCFB04 (hc LEU2 SUI1) / (6)
PMY127 / MATa ura3-52 leu2-3 leu2-112 trp1-63 his4-301(ACG) sui1::hisG pPMB42 (hc LEU2 sui1-I3N) / This study
PMY100 / MATa ura3-52 leu2-3 leu2-112 trp1-63 his4-301(ACG) sui1::hisG pPMB38 (hc LEU2 sui1-K56E) / This study
PMY99 / MATa ura3-52 leu2-3 leu2-112 trp1-63 his4-301(ACG) sui1::hisG pPMB39 (hc LEU2 sui1-K60E) / This study
PMY195 / MATa ura3-52 leu2-3 leu2-112 trp1-63 his4-301(ACG) sui1::hisG p1200 (sc URA3 SUI1) p4389 (hc LEU2 His-SUI1) / This study
PMY179 / MATa ura3-52 leu2-3 leu2-112 trp1-63 his4-301(ACG) sui1::hisG p1200 (sc URA3 SUI1) pPMB52 (hc LEU2 His-sui1-K59A) / This study
PMY180 / MATa ura3-52 leu2-3 leu2-112 trp1-63 his4-301(ACG) sui1::hisG p1200 (sc URA3 SUI1) pPMB53 (hc LEU2 His-sui1-K59E) / This study
PMY181 / MATa ura3-52 leu2-3 leu2-112 trp1-63 his4-301(ACG) sui1::hisG p1200 (sc URA3 SUI1) pPMB54 (hc LEU2 His-sui1-K59D) / This study
PMY182 / MATa ura3-52 leu2-3 leu2-112 trp1-63 his4-301(ACG) sui1::hisG p1200 (sc URA3 SUI1) pPMB55 (hc LEU2 His- sui1-K56E-K60E) / This study
PMY207 / MATa ura3-52 leu2-3 leu2-112 trp1-63 his4-301(ACG) sui1::hisG p4389 (hc LEU2 His- SUI1) / This study
PMY202 / MATa ura3-52 leu2-3 leu2-112 trp1-63 his4-301(ACG) sui1::hisG pPMB52 (hc LEU2 His-sui1-K59A) / This study
PMY203 / MATa ura3-52 leu2-3 leu2-112 trp1-63 his4-301(ACG) sui1::hisG pPMB55 (hc LEU2 His- sui1-K56E-K60E) / This study
PMY03 / MATa ura3-52 leu2-3 leu2-112 trp1-63 his4-301(ACG) sui1::hisG kanMX6:PGAL1-TIF11 p1200 (sc URA3 SUI1) / (6)
PMY212 / MATa ura3-52 leu2-3 leu2-112 trp1-63 his4-301(ACG) sui1::hisG kanMX6:PGAL1-TIF11 p1200 (sc URA3 SUI1) pPMB76 (sc TRP1-TIF11) / This study
PMY214 / MATa ura3-52 leu2-3 leu2-112 trp1-63 his4-301(ACG) sui1::hisG kanMX6:PGAL1-TIF11 p1200 (sc URA3 SUI1) pPMB77 (sc TRP1-tif11-17-21) / This study
PMY236 / MATa ura3-52 leu2-3 leu2-112 trp1-63 his4-301(ACG) sui1::hisG kanMX6:PGAL1-TIF11 pPMB76 (sc TRP1-TIF11) pJCB101 (sc LEU2 SUI1) / This study
PMY237 / MATa ura3-52 leu2-3 leu2-112 trp1-63 his4-301(ACG) sui1::hisG kanMX6:PGAL1-TIF11 pPMB76 (sc TRP1-TIF11) pPMB35 (lc LEU2 sui1-I3N) / This study
PMY238 / MATa ura3-52 leu2-3 leu2-112 trp1-63 his4-301(ACG) sui1::hisG kanMX6:PGAL1-TIF11 pPMB76 (sc TRP1-TIF11) pPMB01 (sc LEU2 sui1-K56E) / This study
PMY239 / MATa ura3-52 leu2-3 leu2-112 trp1-63 his4-301(ACG) sui1::hisG kanMX6:PGAL1-TIF11 pPMB76 (sc TRP1-TIF11) pPMB02 (sc LEU2 sui1-K60E) / This study
PMY246 / MATa ura3-52 leu2-3 leu2-112 trp1-63 his4-301(ACG) sui1::hisG kanMX6:PGAL1-TIF11 pPMB77 (sc TRP1-tif11-17-21) pJCB101 (sc LEU2 SUI1) / This study
PMY247 / MATa ura3-52 leu2-3 leu2-112 trp1-63 his4-301(ACG) sui1::hisG kanMX6:PGAL1-TIF11 pPMB77 (sc TRP1-tif11-17-21) pPMB35 (lc LEU2 sui1-I3N) / This study
PMY248 / MATa ura3-52 leu2-3 leu2-112 trp1-63 his4-301(ACG) sui1::hisG kanMX6:PGAL1-TIF11 pPMB77 (sc TRP1-tif11-17-21) pPMB01 (sc LEU2 sui1-K56E) / This study
PMY249 / MATa ura3-52 leu2-3 leu2-112 trp1-63 his4-301(ACG) sui1::hisG kanMX6:PGAL1-TIF11 pPMB77 (sc TRP1-tif11-17-21) pPMB02 (sc LEU2 sui1-K60E) / This study
PMY263 / MATa ura3-52 leu2-3 leu2-112 trp1-63 his4-301(ACG) sui1::hisG kanMX6:PGAL1-TIF11 p1200 (sc URA3 SUI1) pPMB77 (sc TRP1-tif11-17-21) pCFB03 (sc LEU2 His-SUI1) / This study
PMY264 / MATa ura3-52 leu2-3 leu2-112 trp1-63 his4-301(ACG) sui1::hisG kanMX6:PGAL1-TIF11 p1200 (sc URA3 SUI1) pPMB77 (sc TRP1-tif11-17-21) pPMB47 (sc LEU2 His-sui1-K59A) / This study
PMY265 / MATa ura3-52 leu2-3 leu2-112 trp1-63 his4-301(ACG) sui1::hisG kanMX6:PGAL1-TIF11 p1200 (sc URA3 SUI1) pPMB77 (sc TRP1-tif11-17-21) pPMB48 (sc LEU2 His-sui1-K59E) / This study
PMY266 / MATa ura3-52 leu2-3 leu2-112 trp1-63 his4-301(ACG) sui1::hisG kanMX6:PGAL1-TIF11 p1200 (sc URA3 SUI1) pPMB77 (sc TRP1-tif11-17-21) pPMB49 (sc LEU2 His-sui1-K59D) / This study
PMY267 / MATa ura3-52 leu2-3 leu2-112 trp1-63 his4-301(ACG) sui1::hisG kanMX6:PGAL1-TIF11 p1200 (sc URA3 SUI1) pPMB77 (sc TRP1-tif11-17-21) pPMB50 (sc LEU2 His-sui1-K56E-K60E) / This study
CHY01 / MATa ura3-52 leu2-3 leu2-112 trp163 gcn2sui1::hisG
(TRP1 GCN4-lacZ ) p1200 (sc URA3 SUI1) / (5)
PMY275 / MATa ura3-52 leu2-3 leu2-112 trp163 gcn2sui1::hisG
(TRP1 GCN4-lacZ ) pJCB101 (sc LEU2 SUI1) / This study
PMY276 / MATa ura3-52 leu2-3 leu2-112 trp163 gcn2sui1::hisG
(TRP1 GCN4-lacZ ) pPMB35 (lc LEU2 sui1-I3N) / This study
PMY277 / MATa ura3-52 leu2-3 leu2-112 trp163 gcn2sui1::hisG
(TRP1 GCN4-lacZ ) pPMB01 (sc LEU2 sui1-K56E) / This study
PMY278 / MATa ura3-52 leu2-3 leu2-112 trp163 gcn2sui1::hisG
(TRP1 GCN4-lacZ ) pPMB02 (sc LEU2 sui1-K60E) / This study
PMY164 / MATa ura3-52 leu2-3 leu2-112 trp1-63 his4-301(ACG) sui1::hisG pPMB56 (sc LEU2 sui1-N34A) / This study
PMY165 / MATa ura3-52 leu2-3 leu2-112 trp1-63 his4-301(ACG) sui1::hisG pPMB57 (sc LEU2 sui1-N34E) / This study
PMY166 / MATa ura3-52 leu2-3 leu2-112 trp1-63 his4-301(ACG) sui1::hisG pPMB58 (sc LEU2 sui1-G35E) / This study
PMY167 / MATa ura3-52 leu2-3 leu2-112 trp1-63 his4-301(ACG) sui1::hisG pPMB59 (sc LEU2 sui1-G35R) / This study
PMY168 / MATa ura3-52 leu2-3 leu2-112 trp1-63 his4-301(ACG) sui1::hisG pPMB60 (sc LEU2 sui1-R36A) / This study
PMY169 / MATa ura3-52 leu2-3 leu2-112 trp1-63 his4-301(ACG) sui1::hisG pPMB61 (sc LEU2 sui1-R36G) / This study
PMY170 / MATa ura3-52 leu2-3 leu2-112 trp1-63 his4-301(ACG) sui1::hisG pPMB62 (sc LEU2 sui1-R36E) / This study
PMY171 / MATa ura3-52 leu2-3 leu2-112 trp1-63 his4-301(ACG) sui1::hisG pPMB63 (sc LEU2 sui1-K37A) / This study
PMY208 / MATa ura3-52 leu2-3 leu2-112 trp1-63 his4-301(ACG) sui1::hisG pPMB64 (sc LEU2 sui1-R33A) / This study
PMY173 / MATa ura3-52 leu2-3 leu2-112 trp1-63 his4-301(ACG) sui1::hisG p1200 (sc URA3 SUI1) pPMB65 (sc LEU2 His-sui1-K37E) / This study
PMY210 / MATa ura3-52 leu2-3 leu2-112 trp1-63 his4-301(ACG) sui1::hisG p1200 (sc URA3 SUI1) pPMB68 (sc LEU2 His-sui1-R33E) / This study
PMY204 / MATa ura3-52 leu2-3 leu2-112 trp1-63 his4-301(ACG) sui1::hisG pPMB74 (hc LEU2 His-sui1-K37E) / This study
PMY200 / MATa ura3-52 leu2-3 leu2-112 trp1-63 his4-301(ACG) sui1::hisG pPMB71 (hc LEU2 sui1-R36E) / This study
PMY201 / MATa ura3-52 leu2-3 leu2-112 trp1-63 his4-301(ACG) sui1::hisG pPMB72 (hc LEU2 sui1-K37A) / This study
PMY209 / MATa ura3-52 leu2-3 leu2-112 trp1-63 his4-301(ACG) sui1::hisG pPMB73 (hc LEU2 sui1-R33A) / This study
PMY243 / MATa ura3-52 leu2-3 leu2-112 trp1-63 his4-301(ACG) sui1::hisG kanMX6:PGAL1-TIF11 pPMB76 (sc TRP1-TIF11) pPMB62 (sc LEU2 sui1-R36E) / This study
PMY244 / MATa ura3-52 leu2-3 leu2-112 trp1-63 his4-301(ACG) sui1::hisG kanMX6:PGAL1-TIF11 pPMB76 (sc TRP1-TIF11) pPMB63 (sc LEU2 sui1-K37A) / This study
PMY245 / MATa ura3-52 leu2-3 leu2-112 trp1-63 his4-301(ACG) sui1::hisG kanMX6:PGAL1-TIF11 pPMB76 (sc TRP1-TIF11) pPMB64 (sc LEU2 sui1-R33A) / This study
PMY253 / MATa ura3-52 leu2-3 leu2-112 trp1-63 his4-301(ACG) sui1::hisG kanMX6:PGAL1-TIF11 pPMB77 (sc TRP1-tif11-17-21) pPMB62 (sc LEU2 sui1-R36E) / This study
PMY254 / MATa ura3-52 leu2-3 leu2-112 trp1-63 his4-301(ACG) sui1::hisG kanMX6:PGAL1-TIF11 pPMB77 (sc TRP1-tif11-17-21) pPMB63 (sc LEU2 sui1-K37A) / This study
PMY255 / MATa ura3-52 leu2-3 leu2-112 trp1-63 his4-301(ACG) sui1::hisG kanMX6:PGAL1-TIF11 pPMB77 (sc TRP1-tif11-17-21) pPMB64 (sc LEU2 sui1-R33A) / This study

Table S3. Primers used in this study

Substitution / Sequence 5’ -3’ / Plasmid
I3N / GATATACATATGTCCAATGAGAATCTGAAATC / pPMB111, 96
I3N / CATTTCAGATTCTCATTGGACATATGTATATC / pPMB111, 96
K60E / CTTAAGGTCCTAAAGGAGGACTTTGCATG / pPMB112, 97
K60E / CATGCAAAGTCCTCCTTTAGGACCTTAAG / pPMB112, 97
K56D / GATTTAAAGAGAATTCTTGACGTCCTAAAGAAGGACTTTG / pPMB36
K56D / CAAAGTCCTTCTTTAGGACGTCAAGAATTCTCTTTAAATC / pPMB36
K60D / CTTAAGGTCCTAAAGGACGACTTTGCATG / pPMB37
K60D / CATGCAAAGTCGTCCTTTAGGACCTTAAG / pPMB37
K60A / CTTAAGGTCCTAAAGGCGGACTTTGCATG / pPMB88
K60A / CATGCAAAGTCCGCCTTTAGGACCTTAAG / pPMB88
K59A / CTTAAGGTCCTAGCGAAGGACTTTGC / pPMB47
K59A / GCAAAGTCCTTCGCTAGGACCTTAAG / pPMB47
K59E / CTTAAGGTCCTAGAGAAGGACTTTGC / pPMB48
K59E / GCAAAGTCCTTCTCTAGGACCTTAAG / pPMB48
K59D / CTTAAGGTCCTAGACAAGGACTTTGC / pPMB49
K59D / GCAAAGTCCTTGTCTAGGACCTTAAG / pPMB49
K56E, K60E / AAGAGAATTCTTGAGGTCCTAAAGGAGGACTTTGCATG / pPMB50
K56E, K60E / CATGCAAAGTCCTCCTTTAGGACCTCAAGAATTCTCTT / pPMB50
N34A / GTATCCAACAGAGAGCTGGTAGAAAAACTTTAAC / pPMB56
N34A / GTTAAAGTTTTTCTACCAGCTCTCTGTTGGATAC / pPMB56
N34E / GTATCCAACAGAGAGAAGGTAGAAAAACTTTAAC / pPMB57
N34E / GTTAAAGTTTTTCTACCTTCTCTCTGTTGGATAC / pPMB57
G35E / CCAACAGAGAAATGAGAGAAAAACTTTAAC / pPMB58
G35E / GTTAAAGTTTTTCTCTCATTTCTCTGTTGG / pPMB58
G35R / CCAACAGAGAAATCGTAGAAAAACTTTAAC / pPMB59
G35R / GTTAAAGTTTTTCTACGATTTCTCTGTTGG / pPMB59
R36A / CAACAGAGAAATGGTGCTAAAACTTTAACTAC / pPMB60
R36A / GTAGTTAAAGTTTTAGCACCATTTCTCTGTTG / pPMB60
R36G / CAACAGAGAAATGGTGGAAAAACTTTAACTAC / pPMB61
R36G / GTAGTTAAAGTTTTTCCACCATTTCTCTGTTG / pPMB61
R36E / CAACAGAGAAATGGTGAGAAAACTTTAACTAC / pPMB62
R36E / GTAGTTAAAGTTTTCTCACCATTTCTCTGTTG / pPMB62
K37A / GAGAAATGGTAGAGCAACTTTAACTACGG / pPMB63
K37A / CCGTAGTTAAAGTTGCTCTACCATTTCTC / pPMB63
R33A / CGTATCCAACAGGCAAATGGTAGAAAAAC / pPMB64, 114, 99
R33A / GTTTTTCTACCATTTGCCTGTTGGATACG / pPMB64, 114, 99
K37E / GAGAAATGGTAGAGAAACTTTAACTACGG / pPMB65, 115, 100
K37E / CCGTAGTTAAAGTTTCTCTACCATTTCTC / pPMB65, 115, 100
R33E / CGTATCCAACAGGAAAATGGTAGAAAAAC / pPMB68
R33E / GTTTTTCTACCATTTTCCTGTTGGATACG / pPMB68

REFERENCES FOR SUPPLEMENTAL DATA

1.Gietz, R. D., and Sugino, A. (1988) New yeast-Escherichia coli shuttle vectors constructed with in vitro mutagenized yeast genes lacking six-base pair restriction sites. Gene 74, 527-534

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3.Yoon, H. J., and Donahue, T. F. (1992) The sui1 suppressor locus in Saccharomyces cerevisiae encodes a translation factor that functions during tRNAiMet recognition of the start codon. Mol. Cell. Biol. 12, 248-260

4.Parent, S. A., Fenimore, C. M., and Bostian, K. A. (1985) Vector systems for the expression, analysis and cloning of DNA sequences in S. cerevisiae. Yeast 1, 83-138

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6.Martin-Marcos, P., Cheung, Y. N., and Hinnebusch, A. G. (2011) Functional elements in initiation factors 1, 1A and 2{beta} discriminate against poor AUG context and non-AUG start codons. Mol Cell Biol

7.Donahue, T. F., and Cigan, A. M. (1988) Genetic selection for mutations that reduce or abolish ribosomal recognition of the HIS4 translational initiator region. Mol Cell Biol 8, 2955-2963

8.Hinnebusch, A. G. (1985) A hierarchy of trans-acting factors modulate translation of an activator of amino acid biosynthetic genes in Saccharomyces cerevisiae. Mol Cell Biol 5, 2349-2360

9.Asano, K., Krishnamoorthy, T., Phan, L., Pavitt, G. D., and Hinnebusch, A. G. (1999) Conserved bipartite motifs in yeast eIF5 and eIF2B, GTPase-activating and GDP-GTP exchange factors in translation initiation, mediate binding to their common substrate eIF2. EMBO J 18, 1673-1688

10.Choi, S. K., Olsen, D. S., Roll-Mecak, A., Martung, A., Remo, K. L., Burley, S. K., Hinnebusch, A. G., and Dever, T. E. (2000) Physical and functional interaction between the eukaryotic orthologs of prokaryotic translation initiation factors IF1 and IF2. Mol Cell Biol 20, 7183-7191

11.Fekete, C. A., Mitchell, S. F., Cherkasova, V. A., Applefield, D., Algire, M. A., Maag, D., Saini, A., Lorsch, J. R., and Hinnebusch, A. G. (2007) N-and C-terminal residues of eIF1A have opposing effects on the fidelity of start codon selection. EMBO 26, 1602-1614

12.Reibarkh, M., Yamamoto, Y., Singh, C. R., del Rio, F., Fahmy, A., Lee, B., Luna, R. E., Ii, M., Wagner, G., and Asano, K. (2008) Eukaryotic initiation factor (eIF) 1 carries two distinct eIF5-binding faces important for multifactor assembly and AUG selection. J Biol Chem 283, 1094-1103

13.Acker, M. G., Kolitz, S. E., Mitchell, S. F., Nanda, J. S., and Lorsch, J. R. (2007) Reconstitution of yeast translation initiation. Methods Enzymol 430, 111-145

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